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Citations for Becton, Dickinson and Company :
4851 - 4900 of 5708 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... catheter access was achieved by placing conscious animals in a rodent restrainer and using 1 mL tuberculin slip-tip syringes (cat. no. 309626, BD) with attached blunt 22 ga dispensing needles (cat ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... mononuclear cell suspensions were incubated with BD Horizon™ Fixable Viability Stain 780 (FVS780, BDBiosciences, Cat# 565388, Dilution: 1:4000) in PBS (Ca+ and Mg+ free ...
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bioRxiv - Evolutionary Biology 2022Quote: ... BV421-conjugated anti-rat CD45RA (clone OX-33, Cat#740043), and BV605-conjugated anti-rat CD45 (clone OX-1, Cat#740515) from BD Biosciences (Franklin Lakes ...
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bioRxiv - Developmental Biology 2022Quote: ... A density of 100,000 cells per well was used for culture in 8-well #1 coverglass chambers (Labtek) precoated with growth factor reduced Matrigel (80µl, BD Biosciences) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated at 106 cells/ml in 6-well plates plate-coated with anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) (1 μg/ml each, BD Biosciences) by pre-coating the wells with 100 μg/ml goat anti-hamster IgG (Cappel).
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bioRxiv - Immunology 2021Quote: ... fixed in PBS with 1% paraformaldehyde and analyzed by flow cytometry (BD Accuri™ C6 Plus Flow Cytometer, BD Biosciences), measuring cell-surface CD4 ...
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bioRxiv - Immunology 2019Quote: Indirect immunofluorescence was performed on frozen fixed (acetone:methanol) liver tissue using monoclonal antibody CD45-FITC (1:100) (30-F11, BD Pharmingen). Co-localisation was assessed using CD147 (1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... SSEA3 conjugated to Phycoerythrin and TRA-1-60 conjugated to Alexa Fluor™ 647 (all of them from BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... the monocytes were incubated for 10 min at room temperature with human BD Fc-block (2.5 µg per 1×106 cells in 100 µL, Cat. no. 564220, BD biosciences). The cells were further incubated with the following antibody cocktail for 30 min at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... or saline were infused directly into the tumor at a flow rate of 10?μl min-1 via a Genie Touch syringe pump through PE10 catheter tubing and a 30-gauge needle (BD). Injection at the focal zone was confirmed via B-mode imaging by locating the needle tip ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked with BSA and incubated with primary antibodies diluted 1/1000 against Cdh5/VE-cadherin (BD Biosciences 555289), Phospho-Akt (Cell Signalling ...
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bioRxiv - Cancer Biology 2019Quote: Effect of Grem-1 in the invasion of cells were assayed using the BD BioCoat Matrigel Invasion Chambers (BD Bioscience). Each well of a 24-well plate contained an insert with an 8-mm pore size PET (polyethylene terephthalate ...
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bioRxiv - Immunology 2020Quote: NK cells were co-cultured for 5 hours with K562 or GSCs target cells at an optimized effector:target ratio of 5:1 together with CD107a PE-CF594 (BD Biosciences), monensin (BD GolgiStopTM ...
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bioRxiv - Immunology 2020Quote: ... or WNV MPs (1 µg/mL) or DMSO (0.1%) as negative control together with Brefeldin A (BD GolgiPlug, BD Biosciences) for 6 hours ...
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bioRxiv - Immunology 2020Quote: ... or WNV MPs (1 µg/mL) or DMSO (0.1%) as negative control together with Brefeldin A (BD GolgiPlug, BD Biosciences) for 6 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei enriched in Nuclei Suspension Buffer were stained with DAPI (1:1000) for nuclei isolation using the Influx cell sorter (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 30 min at 4°C with antibodies specific for the following markers: CD45.2-PE-Cy7 (1:200 dilution; BD Biosciences); EpCAM-APC (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: Flow cytometry of anti-p-ERK-stained CRC cells resuspended in PBS/1%BSA cells was done using an Accuri cytometer (BD). Cells were gated for populations displaying different tdTomato fluorescence values (negative ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... FCRL6+/FCRL6- pro B (AA4.1+Lin-CD43+B220+CD19-IgM-) cells from the FL and BM were sorted using a FACSAria II sorter (BD).
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bioRxiv - Immunology 2019Quote: ... Single suspensions of FL cells were prepared by crushing the tissue with a 1 ml syringe plunger and passage through a 40 μm strainer (BD). Single cell suspensions were treated with ACK lysing buffer and re-suspended with PBS containing 2% FCS ...
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bioRxiv - Immunology 2019Quote: ... Cell surface markers were immunolabeled with anti-Gr-1 (PE Rat Anti-Mouse Ly6G and Ly6C, BD Pharmingen, Franklin, NJ), F4/80 (Anti-F4/80 PE ...
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bioRxiv - Genetics 2019Quote: ... a second set of antibodies was used (volume per 1×10^6 cells, all from BD Biosciences, unless stated otherwise): CD45 V500 (4μl ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed 3 times with PBS-T and 50μl of either biotinylated rat anti-mouse IgG1 (1:500, BD Bioscience), rat anti-mouse IgG2a (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor xenografts were established in 6-week-old NOD SCID mice via subcutaneous injection into the dorsal region of each mouse with 1 x 107 cells/0.15 ml Hank’s balanced salt solution containing Matrigel® (BD Biosciences). Experiments were performed at five mice in each group (vector control ...
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bioRxiv - Cell Biology 2020Quote: ... at 3:100 or phospho-AKT (pS473) at 1:20 dilutions for 30 mins at RT before analysis on FACSAria II (BD).
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bioRxiv - Cancer Biology 2019Quote: ... the apoptosis of PANC-1 cells was determined by using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences). The cells were harvested with trypsin-EDTA (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and microglia (CD45med CD11b+were sorted into collection tubes coated with FCS and filled with complete RPMI 1640 medium (1% penicillin, streptomycin, 0.1% 2-ME) with 20% FCS using a FACSAria device (BD Biosciences). Purity of sorted populations was routinely above 95% ...
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bioRxiv - Developmental Biology 2021Quote: ... Phenotyping BCAMhi and BCAMlo organoids: Single cells were incubated with anti-human BCAM BV421 (clone B64; 1:50; BD Biosciences), EGFR BV605 ...
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bioRxiv - Cell Biology 2021Quote: Human THP-1 cells (NCTC #88081201) were maintained or passaged in 25 or 75 cm2 tissue culture flasks (BD Biosciences) using RPMI-1640 (Lonza ...
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bioRxiv - Bioengineering 2020Quote: ... we preincubated 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were activated by seeding in 96-well plates pre-coated with anti-mouse CD3e (1 μg/ml working concentration, Clone: 145-2C11, BD) and anti-mouse CD28 (2 μg/ml working concentration ...
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bioRxiv - Bioengineering 2021Quote: ... at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml, Clone: 145-2C11, BD Pharmingen).
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bioRxiv - Bioengineering 2021Quote: ... Cells were plated at a concentration of 150,000 cells per 6-well in mTeSR-1 with 10 uM Y27632 (BD Biosciences). The next day ...
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bioRxiv - Biochemistry 2020Quote: ... The resins were pre-treated with yeast extract solution (0.1 g/100 ml H2O for 1 hour; BD Biosciences, NJ) to avoid non-specific binding of EVs to resins ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% TritonX100 in PBS for 20 min at RT and stained overnight with rat-anti-PECAM1/CD31 antibodies (1/100, clone Mec13.3, BD Pharmingen) in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were further incubated for 1 hr in solution containing goat anti-rabbit antibody conjugated with FITC (BD Biosciences, USA). All steps were carried out at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of the cell suspension was adjusted to 2×106 cells ml-1 and subsequently aspirated into a 3 ml luer-lock syringe (BD).
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bioRxiv - Systems Biology 2021Quote: ... Similarly detached hCMEC/D3 cells were incubated with APC-conjugated mouse monoclonal anti-BCRP (1:100, BD Biosciences, Oxford, UK), or PE-conjugated mouse monoclonal anti-MDR1A (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed with ice-cold 1% paraformaldehyde (PFA) in PBS and analyzed via flow cytometry using an LSRII SORP flow cytometer (BD) and DIVA software (BD).
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with primary antibodies (Guinea-pig anti-VGAT, Synaptic Systems 131004, 1μg/mL, 1:200 and mouse anti-mGluR1, BD Pharmingen,0.5mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Immunocytochemical staining using a rat monoclonal anti-mouse CD31 antibody (1:100; clone MEC 13.3; BD Pharmingen, San Jose, CA) was used to detect endothelial cells in the mouse heart ...
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bioRxiv - Immunology 2019Quote: ... Each obtained monomeric H-2Db/peptide complex (0.5 mg/ml) was tetramerized at a 4:1 ratio with streptavidin-PE (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... lung single cell suspensions were stained with tetramer prior to the surface and intracellular staining for 1 hour at 37°C.Intracellular cytokine staining was performed using the BD Cytofix/Cytoperm kit (BD Biosciences) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... FBS and stained on ice for 1 hr with the panel of fluorescent antibodies against IgM ([clone G20-127] BD), CD4 ([clone OKT-4] BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... buffer (PBS containing 0.1% sodium azide and 1% bovine serum albumin) and fixed/permeabilized using a Cytofix/Cytoperm Buffer Kit (BD Biosciences) or a FoxP3 Transcription Factor Buffer Kit (eBioscience) ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 300µL of PBS + 1% v/v FBS and 150µL were added to 2µL of Mouse BD Fc Block (BD Biosciences 553142). Homogenates in Fc block were incubated for 15 minutes at 4°C ...
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bioRxiv - Genomics 2020Quote: Primary human bronchial epithelial basal cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sections were then fixed in 4% PFA and incubated with primary antibodies: anti-CD31 (1:50, BD Pharmigen; RRID: AB_393571), anti-CD42b (1:75 ...