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Citations for Becton, Dickinson and Company :
4201 - 4247 of 4247 citations for FAS Associated Death Domain Protein FADD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... 1x106 cells in 100 μL were stained for 30 minutes at room temperature using the following antibodies: CD90-BUV395 (BD, #740260, 0.1 mg/μL), ECAD-AF405 (Novus Bio ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in TBS/Tw and then incubated overnight with primary antibody Syn-1 (BD Biosciences #610,787, 1:1000) or MJFR-14-6-4-2 (Abcam #209,538 ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Microbiology 2023Quote: Surface markers of splenocytes were identified using monoclonal antibodies in conjunction with two immunofluorescence analysis with a flow cytometer (BD Accuri C6 Plus: BD, Mountain View, CA, USA). Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1 ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes were cultured in RPMI supplemented with 10% FBS and 50 μM beta-mercaptoethanol (RPMI media) Lymphoblasts were generated by activation with plate bound murine anti-CD3ε antibody (BD Biosciences, Clone: 145-2C11) in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163 ...
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bioRxiv - Immunology 2024Quote: ... was prepared by coating the well with 50 μl of 10 μg/ml of anti-mouse IFNγ monoclonal capture antibody (BD Biosciences; cat. no. 551309) in PBS and allowed to incubate at 4°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBST and 50 μl of 1 μg/ml of the anti-mouse IFNγ monoclonal detection antibody conjugated to biotin (BD Biosciences; cat. no. 551506) in PBS was added and allowed to incubate at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 30 minutes at room temperature with a rat monoclonal antibody anti-Mouse CD8 (BD Pharmingen, BD Bioscience, San Jose, CA, USA) diluted 1:50 in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... and the cell suspensions purity was higher than 90% as assessed by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were washed three times with PBS and CD34+ cells were isolated as described 35 using rhesus macaque-specific antibodies to CD34 (clone 563; BD Pharmingen, San Jose, CA, USA) and the anti-PE MACS magnetic bead system (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... The fluorescent signal from GLI1 OE and control cell samples stained for cTnT-positivity was normalized to fluorescent signal from cells stained with a BV421-conjugated isotype control IgG antibody (BD Biosciences 562438, Lot 8242926, X40). Fluorescence was quantified ...
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bioRxiv - Immunology 2020Quote: ... Single cells were stained with the different fluorescent antibodies (as indicated in the figure legends) and DsRed on the BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA). Analyses were completed using FlowJo analysis software (FlowJo ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained for 30 min at 4°C under darkened conditions with the following fluorochrome labeled monoclonal antibodies: anti-CD4-AmCyan (Clone SK3, BD Biosciences, Franklin Lakes, Nj, USA), anti-CD25-APCCy7 (Clone M-A251 ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were rinsed with PBS and incubated for 20 hours at 4°C in primary antibody solution 1:3000 mouse anti-Ki67 (BD Biosciences, San Jose, CA, USA), 2% normal horse serum ...
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bioRxiv - Cancer Biology 2019Quote: ... and cells in the S phase using incubation with bromodeoxyuridine (BrdU) for 1 h and labeling with an anti-BrdU antibody (APC BrdU flow kit, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were probed with different combinations of conjugated primary antibodies (Table S1) and analyzed by flow cytometry (Canto II AIG, BD Biosciences, Franklin Lakes, NJ, USA). Finally ...
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bioRxiv - Immunology 2020Quote: ... The purified CD4+ T cells were seeded at 1 × 105 cells/well in the anti-CD3 monoclonal antibody (mAb) (10 μg/mL, BD Pharmingen, Franklin Lakes, NJ, USA) precoated 96-well tissue culture-treated plate ...
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bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Jurkat cells were maintained in culture for 1 week using enhanced RPMI and screened for TCR expression (Jing et al., 2016) using an anti-mouse TCR β chain (mTCRBC) APC antibody (BD Biosciences, San Jose, CA) and tetramer staining as described above.
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bioRxiv - Microbiology 2021Quote: The following mouse and human monoclonal antibodies were utilized for virion capture assays: anti-PSGL-1 and anti-mouse IgG (BD Biosciences, Cat# 556053 and 557273) and anti-gp120s (clones 2G12 and PG9 acquired from the NIH ARP ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (BD Pharmingen 553673 2 μg/ml) at 4 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with multiple flow cytometry validated antibody cocktails (see below) and analyzed using either BD Fortessa or LSRII cytometers (BD Horizon, BD, Franklin Lakes, NJ). Data were analyzed and quantified using FlowJo v10.6 software (RRID:SCR_008520) ...
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Cell Biology 2023Quote: ... Other antibodies and chemicals were obtained from the following manufacturers: anti-AIM-1 (AURKB) mouse monoclonal (6/AIM-1) antibodies (#611082) (BD Biosciences, Franklin Lakes, NJ, USA); anti-GFP polyclonal antibodies (#598 ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 × 106 cell/mL cells were stained with the appropriate antibodies and analyzed by flow cytometry (BD FACS Fortessa, BD Biosciences, San Jose, CA, USA). Fluorescence minus one (FMOs ...
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bioRxiv - Microbiology 2024Quote: ... The amount of coating gp120 was optimized through competition by binding of the Leu3A (anti-CD4) antibody (clone SK3; Catalog no. 340133; Final dilution 1:5; BD Bioscience, San Jose, CA, USA). Cryopreserved peripheral blood mononuclear cells ...
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bioRxiv - Cell Biology 2019Quote: ... at room temperature for 10 min and then incubated with Alexa Fluor® 488 Mouse anti-H2AX (pS139) antibody (clone N1-431, BD Pharmingen™, Franklin Lakes, NJ), diluted 1:1000 with 1% bovine serum albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... USA) and the lower half with a primary 42-kDa anti-β-actin antibody (chicken, ab-5, anti-mouse IgG, BD Biosciences, San Jose, CA, USA). Both antibodies were diluted 1 ...
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bioRxiv - Microbiology 2020Quote: ... enzyme-linked immunosorbent spot (ELISpot) assays were performed using the Mouse IFN-γ ELISPOT Pair kit including capture and detection antibody (BD Bioscience, Franklin Lakes, NJ, USA) and HRP Streptavidin (BD Bioscience ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated with phycoerythrin (PE)-conjugated Enpep antibody at 4°C for 60 min and then sorted by FACScan flow cytometry (Becton, Dickinson and company, Franklin Lakes, NJ).
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bioRxiv - Developmental Biology 2020Quote: ... The percentage of cTnT+ cells was calculated by dissociating D8 or D10 CMs and staining with a BV421-conjugated anti-cTnT antibody (BD Biosciences 565618, Lot 8136651, 13-11). The fluorescent signal from GLI1 OE and control cell samples stained for cTnT-positivity was normalized to fluorescent signal from cells stained with a BV421-conjugated isotype control IgG antibody (BD Biosciences 562438 ...
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bioRxiv - Microbiology 2021Quote: ... Signals for 5 fluorescent markers were created by staining with antibodies against the common leukocyte antigen (Rat-anti-mouse CD45, Cat# 550539, clone: 30-F11, BD Pharmingen, San Jose, CA 95131, USA) indirectly labeled with a goat-anti-rat secondary antibody labeled with Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...
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bioRxiv - Microbiology 2023Quote: ... Grids were blocked with 1% BSA / 1% gelatin / 0.01% Tween20 in PBS for 10 min then labeled with primary antibody (anti-fibrinogen Millipore--Sigma, Marlborough, MA, USA #341552 or anti-TER-119, BD Biosciences, San Jose, CA, USA #550565) diluted 1:25 in blocking buffer for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169; Phosphoserine/threonine clone 22A, BD Transduction Laboratories # 612548) were added to the blocking solution at dilutions ranging from 1/500 to 1,5000 according to the manufacturer’s datasheets ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by staining for 30 min at 4°C with the appropriate antibodies: PE-Cy7 rat anti-mouse CD45 (cat # 561868, BD Biosciences, clone 30-F11, 2 ng/μl), BV421 rat anti-mouse CD11b (cat # 562605 ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD, Franklin Lakes, NJ, USA, cat.: 562440), anti-human CD20 (BioLegend ...