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Citations for Becton, Dickinson and Company :
4101 - 4150 of 4247 citations for FAS Associated Death Domain Protein FADD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were isolated and labelled using biotin-conjugated lineage antibody cocktail in combination with PerCP- conjugated streptavidin (BD, 554064; 1:200), and antibodies against SCA-1 ...
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bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation for 2 hours at room temperature with primary antibody (SOX2 [1:200, EMD Millipore AB5603], NESTIN [1:200, BD Pharmingen #556309]). After washing five times with PBS/0.05% Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... WBM cells were blocked with Fc Blocker and stained with biotinylated-Lineage antibody cocktail according to the Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit by BD (Catalog No. 560492). The stained cells were labelled with streptavidin magnetic particles and placed into BD IMagnet (BD ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Immunology 2020Quote: ... and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Genetics 2020Quote: ... in 100 μl of a 1:200 cocktail of fluorescence-conjugated antibodies to 15 cell surface markers encompassing the major immune lineages B220 (BD, clone RA3-6B2), CD19 (BD ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed with 1%FBS PBS and stained for 20 minutes at room temperature with the following panel of fluorescently conjugated antibodies diluted in 1%FBS PBS: CD56-PECy7 (BD Biosciences, clone NCAM16.2), CD16-PacBlue (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Antibody concentrations were as follows: alveolar macrophage membranes were stained using CD206 PE-CF594 (Mouse Anti-Human CD206, BD Biosciences; catalogue number: 564063) at a concentration of 4µL in a 50μL final staining volume per section ...
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently washed with FACS buffer (1% BSA and 0.1% sodium azide in phosphate-buffered saline) and stained with anti-HLA-A2 specific monoclonal antibody (clone BB7.2) (BD-Pharmingen, San Diego, CA) at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies were used at the indicated dilutions (in 1% BSA): anti-paxillin (clone 249, BD Transduction Labs, USA, 1:400), anti-vinculin (hVin1 ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was removed and cells were resuspended in 50 µL of APC-conjugated anti-CXCR4 antibody (clone 12G5, BD Biosciences, catalogue number 560936) diluted 1:50 in FACS buffer (PBS + 0.5% BSA) ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD Biosciences cat#347580) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:25 ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Physiology 2021Quote: ... Following resuspension in PBS and passage through a 70μm strainer, cells were incubated in Fc block solution (Bio Rad, Cat# BUF041A) and PerCP-conjugated anti-CD11b antibody (BD Pharminogen, Cat# 550993). After PBS washes ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 50 µL Perm/Wash buffer containing a cocktail of anti-human antibodies including IFNγ BV480 (BD, 566100, clone B27), TNFα BV605 (Biolegend ...
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bioRxiv - Genomics 2019Quote: ... fluorescein isothiocyanate (FITC)- mouse anti-human Annexin-V (biolegend; Cat# 640945) and APC-anti-human anti-CD42b (GPIb) monoclonal antibody (BD Pharmingen; Cat# 551061). The calcein AM blue was used to identify live platelet sized events ...
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bioRxiv - Cancer Biology 2022Quote: T-cell degranulation was evaluated by adding an anti-CD107a antibody at the beginning of coculture (CD107a-FITC clone H4A3, BD, Catalog N° 555800) and Golgi Stop (BD ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were resuspended and stained using cell surface antibody markers preconjugated to a fluorophore: KIT (CD117, BD Pharmingen 25-1171-82, RRID: AB_469644), CD45.2 (BD Biosciences 553772 ...
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in FACS Buffer (1:200, PBS, 2% FBS, 2 mM EDTA, 0,05% NaN3) with antibodies recognizing: CD14 PECy7 (555399, BD Biosciences, Franklin Lakes, USA), CD16 BV510 (563830 ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Microbiology 2022Quote: ... was used as primary antibody for samples and mouse anti-clathrin heavy chain clone 23 (1:2,000; BD Biosciences, Franklin Lakes, NJ, USA) for the detection of clathrin heavy chain as a loading control ...
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bioRxiv - Neuroscience 2022Quote: ... Following overnight incubation the plate was washed 3 times with DPBS and then incubated with 20 µl anti-CD45 antibody (BD Pharmingen, catalog #550539) in 12 ml of 0.2% BSA for a minimum of 2 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dispersed into a single cell suspension using 0.05 % trypsin and subsequently incubated with an anti CD144 antibody (anti-VE-Cadherin; BD Biosciences, #562242, 1/50) for 30 min on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Samples are ultracentrifuged and pellets were resuspended and incubated onto 96 well ELISA plates containing 5ug/mL anti-syn42 monoclonal antibody (BD transduction #610-787). ELISAs were carried out on test samples run alongside serial diluted standard synthetic α-Syn ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... pre-coated for 1 hour at 37°C with anti-CD3 antibodies (5ug/mL, clone: 145-2C11, BD Biosciences, San Diego, CA, USA) and supplemented with soluble anti-CD28 antibodies (2ug/mL clone ...
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bioRxiv - Immunology 2024Quote: ... The labeled cells were cultured in complete RPMI-1640 medium plus IL-2 (20 units/ml) or IL-2 and anti-CD3 antibody (1μg/ml) (BD Pharmingen (San Diego, CA), or alternatively plus IL-4 (4ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with multiple flow cytometry validated antibody cocktails (see below) and analyzed using either BD Fortessa or LSRII cytometers (BD Horizon, BD, Franklin Lakes, NJ). Data were analyzed and quantified using FlowJo v10.6 software (RRID:SCR_008520) ...
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bioRxiv - Immunology 2023Quote: ... Surface antibodies were prepared at optimal dilutions (see Supplementary Table S1) in FACS supplemented with Brilliant Stain Plus buffer (BD; Franklin Lakes, NJ), then were added to samples in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... and then blocked in 1% bovine serum albumin in PBST before addition of primary antibodies against E-cadherin (1:200, BD Transduction Laboratories, 610181), laminin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5-μm sections were stained with the following antibodies following citrate antigen retrieval and blocking with 10% normal donkey serum: mouse anti-glutamine synthetase (BD Bioscience, 1:2000), mouse anti-Ki67 (BD ...
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bioRxiv - Developmental Biology 2023Quote: ... pre-incubated with anti-mouse CD16/CD32 (FcRII block) and then stained with the following anti-mouse monoclonal antibodies: GR1 FITC (clone RB6-8C5, BD Biosciences, RRID: AB_394643), F4/80 FITC (clone BM8 ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 4°C for 30 min with a B cell surface marker antibody cocktail (Table S11) with 10 µl Brilliant Stain Buffer Plus (BD, cat. No. 566385) diluted in PBS containing 1% BSA up to a volume of 100 µl ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with flow cytometry antibodies (Supplemental Table 9) in flow cytometry staining buffer (PBS, 1% BSA) and brilliant staining buffer (563794, BD, Franklin Lakes, NJ). Cells were analyzed using a 5-laser (16UV-16V-14B-10YG-8R ...
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bioRxiv - Microbiology 2023Quote: ... we stained cells expressing wild-type and mutant SFV glycoproteins with a mouse anti-SFV immune ascitic fluid (ATCC Cat#: VR-1247AF) or a control mouse IgG2b antibody (BD Biosciences Cat#: 557351). Staining of wild-type and mutant EEEV or SFV E3–E2–(6K/TF)–E1 was detected with a PE-conjugated donkey anti-mouse F(ab’)2 fragment (Jackson ImmunoResearch Cat# ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...