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Citations for Becton, Dickinson and Company :
4101 - 4150 of 8504 citations for 6 Amino 5 2 chloro 4 nitrophenyl azo naphthalene 1 sulfonamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and immunostained with biotinylated antibodies as follows: anti-mouse IgG1 (1:100, BD Pharmingen 553441; A85-1), anti-mouse IgG2b (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... dead cells were excluded with the help a DAPI viability dye (1 μg ml-1, BD Pharmingen). Single cells were gated for based on forward and side scatter parameters ...
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bioRxiv - Genetics 2023Quote: ... 10 g.L-1 of D-glucose (EMD Chemicals) and 15 g.L-1 of Bacto agar (BD Diagnostics)) directly from 15% glycerol (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34-eFluor610 (1:50, cone 4H11; eBioscience) and CD144-V450 (1:50, clone 55-7H1; BD Biosciences). 7AAD (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... After 1 hr incubation on ice bacteria outgrowth was done for 1 hr in Luria-Bertani (BD) prior to selection on LB agar containing desired antibiotic (Sup ...
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bioRxiv - Neuroscience 2019Quote: ... Intracellular cytokine staining was performed according to the manufacturer’s protocol (Cytofix/Cytoperm buffer set from BD with PE-Cy7-conjugated anti-CD4 (RM4-5), eFluor 450-conjugated anti-TCR-β (H57-597) ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
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bioRxiv - Neuroscience 2020Quote: ... infected mouse brain for flow cytometry were obtained as described.(Posel et al., 2016) Isolated cells were incubated overnight (5 hours for microglia analysis) in GolgiPlug and GolgiStop (BD Bioscience #555029 and #554724) in RPMI media containing HEPES and 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
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bioRxiv - Microbiology 2021Quote: Lorikeet intestinal contents collected at necropsy were plated on reduced Trypticase Soy Agar (TSA II) with 5% sheep blood (BD BBL, Franklin Lakes, NJ) and incubated anaerobically (5% CO2 ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were cultured in complete media (106 cells /ml) for 72h in anti-CD3/anti-CD28 (5 μg/mL; BD Biosciences, San Jose, USA) pre-coated 96-flat-well plates (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
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bioRxiv - Immunology 2019Quote: RAMOS cells were resuspended in cR10 media at 5 × 105 cell/mL at a final volume of 10 mL in T25 tissue culture flasks (BD Biosciences, San Diego, CA) and either left untreated or cyclosporine A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Microbiology 2021Quote: ... This was done by treating both the PDMS and glass surface with 5-10 sweeps of a portable laboratory corona device (model BD-20ACV, Electro-Technic Products). After treatment the chip was placed carefully onto the glass slide and gently tapped to facilitate full contact between the PDMS and glass surface ...
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bioRxiv - Immunology 2022Quote: ... the frozen bacterial stock was thawed in 37°C water bath and cultured at 37°C in 5% CO2 in Todd Hewitt broth (BD Biosciences, San Jose, CA) to mid-logarithmic phase ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with ice-cold DPBS plus 5 mM EDTA and stained with annexin V and PI (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Developmental Biology 2023Quote: ... cells filtered into 5 ml polystyrene tubes and analyzed by flow cytometry using a FACS Caliber flow cytometer and FlowJo software (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: Splenocytes from 125Tg B6 and WT B6 control mice were stained with CellTrace Violet and cultured for 3 days35 in complete RPMI alone or stimulated with 5 ug/mL anti-mouse anti-CD40 (BD Pharmingen clone HM40-3) in the presence or absence of conjugates described above at 150 nM concentrations ...
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bioRxiv - Genetics 2024Quote: ... the pigs were fasted overnight and 5∼10 mL blood was sampled from the jugular vein using a vacutainer (Cat no. 367986, BD Vacutainer SST™, USA) and transported to the laboratory within 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... Passages were performed by resuspending Matrigel-embedded organoids in cold DPBS and transferring them to a Falcon tube using a 2 mL syringe with a 27 G ½ needle (BD Microlance #300635) to break them up ...
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bioRxiv - Microbiology 2020Quote: ... Strains were precultured during 48h in 300 μL of Synthetic Defined (SD) medium (Warringer et al., 2011) with some modifications (2% glucose, 0.14% Yeast Nitrogen Base (BD Difco™, U.S.A.), 0.5% ammonium sulfate ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of EDTA-anticoagulated whole blood were stained with the monoclonal antibodies CD3 BV605 (SP34-2, BD Biosciences, San Jose, CA), CD4 PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... The hemocytes were collected from thirty to forty shrimps at 2 hours post injection for each sorting which was performed on a FACSMelody cell sorter (BD Biosciences, USA). The fluorescence boundary was set based on detection of the shrimp hemocyte self-fluorescence without VP injection.
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Pathology 2022Quote: ... the samples were washed and resuspended in PBS supplemented with 2% FBS for analysis on a five-laser flow cytometer (BD Biosciences, Fortessa). The data were analysed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Pathology 2020Quote: ... Tomato-positive and -negative cells differed by ∼2 orders of magnitude in tomato fluorescence with excitation of 554nm and emission of 581nm by Influx™ cell sorter (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: ... for 1 h or 2 h and then subsequently incubated for 30 min on ice with an APC-conjugated rat anti-mouse IgG (BD Biosciences, CA). Stained cells were analyzed using a BD AccuriTM C6 Plus with a Sampler flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Samples were resuspended to a volume 2.5 mL with PBS with 2% FBS and 0.4% EDTA and placed on an Imag (BD Biosciences, San Diego, CA) for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS+2% goat serum and cell cycle analysis was conducted using a BD FACS Calibur (BD Biosciences, UK).
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bioRxiv - Cancer Biology 2020Quote: ... two groups of NOD/SCID female mice were orthotopically injected in the mammary fat pad #4 with 2×106 cells of either ALDH1A3 overexpressing or vector control MDA-MB-231 cells (admixed with high concentration Matrigel, BD, Cat # 354262). Mice were euthanized 49 days post cancer cell implantation and the lungs were harvested ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Cell Biology 2019Quote: ... A second round of purification was then performed with anti-rat Dynabeads coated with rat anti-mouse ICAM-2 (rat clone 3C4; BD Biosciences #553325). Cells were cultured in Endothelial Cell Growth Medium MV2 (PromoCell ...
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences, Inc) and data were analyzed using FlowJo software ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were fixed for 10 min at room temperature with 2% Paraformaldehyde and analysed using LSRFortessa™ X-20 Cell Analyzer (BD Biosciences), LSR II Flow Cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were incubated in 200 μl of a mixture of BD FACS™ Permeabilizing Solution 2 and BD FACS™ Lysing Solution prepared according to manufacturer’s instructions (BD Biosciences) for 8 min at RT ...
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bioRxiv - Immunology 2022Quote: ... then fixed with 2% paraformalin and kept on ice until acquisition on a BD Symphony A3 (Becton Dickinson BD; Franklin Lakes, NJ). Data was analyzed using FlowJo 10.8.1 (RRID:SCR_008520 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with 2% FACS buffer with 50nM dasatinib and fixed using fixation/permeabilization solution (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the internal space of the chambers in which biofilms were cultivated measured 5000μm x 500μm x 70μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417-11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1mL syringes (Brandzig ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...