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Citations for Becton, Dickinson and Company :
4001 - 4050 of 8504 citations for 6 Amino 5 2 chloro 4 nitrophenyl azo naphthalene 1 sulfonamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
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bioRxiv - Microbiology 2021Quote: ... The stained cells were washed and resuspended in PBS containing 2% FBS before being strained through a 70-μm cell mesh filter (BD Biosciences). RBD-specific single B cells were gated as CD19+CD27+CD3−CD14− CD56−IgM−IgD−IgG+RBD+ and sorted into 96-well PCR plates containing 10 μL of RNAase-inhibiting RT-PCR catch buffer (1M Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Cells were fixed in 2% paraformaldehyde and GFP fluorescence was measured using the BD Accuri™ C6 flow cytometer (BD Biosciences). Flow sorting was performed on unfixed cells the FACS Aria III (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... APC-R700 rat anti-mouse IL-2 (Cat No.-565186) and PE-CF594-rat anti-mouse IFN-γ were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACS Aria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... samples of 1×106 per mL pooled CAR T cells with 2×106 per mL Nalm 6 were centrifuged at 300 × g for 2 min in 96-well flat-bottom plates and incubated in HTCM with 1x Brefeldin-A/GolgiPlug (BDBiosciences #555029), 1x Monensin/GolgiStop (BD Biosciences #554724) ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ T cells or MT-2 cells were activated with media containing 50 ng/mL anti-CD3 (eBioscience) and 100 ng/mL anti-CD28 (BD Biosciences) antibodies.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 0.5 mL PBS to achieve a final density of 2×105 cells prior to acquisition and were then analyzed by a FACSCalibur flow cytometer (BD Biosciences). The surface marker expression of human MSCs thus enriched was assessed by using flow cytometry following 2 passages ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were later suspended in FACS buffer (98% PBS, 2% FBS) and analyzed by flow cytometry (BD LSR-Fortessa X-20). After the sample data was acquired ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Written informed consent was provided by study participants and/or their legal guardians and 2-5mL of venous blood were collected into CPD vacutainer tubes (BD Vacutainer). Alternatively ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were washed 2 times in FCM Blocking Buffer then analyzed on a BD Accuri C6 Flow Cytometer (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with PBS in the presence of 2 % BSA and incubated with anti-IFN-γ-PE-CY7 (BD Biosciences), anti-IL-2-APC (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed twice with FACS buffer and strained through a 100 μm filter before sorting CD11b+ microglia and ACSA-2+ astrocytes on a FACS Aria III (BD Biosciences) with a 100 μm nozzle ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed once in a large volume of PBS and post-fixated in 2% PFA in PBS before analysis on an LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing software (version 2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of RBD to hACE-2 on the cell surface was assessed via the mean fluorescent intensity measured by flow cytometry using BD LSRFortessa (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with drugs or DMSO 5 days after infection and the fraction of TUBB-expressing cells was measured 3 days after treatment and then every 2 days as the fraction of mCherry-positive cells by flow cytometry on an LSR-II flow cytometer (BD Biosciences). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed 3 times with PBS with Ca2+ and Mg2+ containing 2% FBS and mean fluorescence intensity per cell was measured by LSRII (BD Biosciences) flow cytometer the same day of the assay.
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bioRxiv - Developmental Biology 2022Quote: ... 2% FBS in Ca2+/Mg2+-free PBS) and DAPI negative cells were selected by cell sorting using FACSAria Fusion (BD Biosciences). Cell viability was above 70% for each sample ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with FACS buffer and fixed with 2% paraformaldehyde for 1hr prior to analysis on an LSRII flow cytometer (BD Biosciences). When stain sets contained tetramers ...
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bioRxiv - Developmental Biology 2020Quote: ... AAV9-mediated Snhg1 or NC were delivered by intracardiac injection into the left ventricle in 7-day-old mice at a dose of 2 × 1010 viral genome particles per animal using an insulin syringe with a 30-gauge needle (BD Biosciences). And the adult mice received the injection at a dose of 1 × 1011 viral genome particles per animal ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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bioRxiv - Microbiology 2021Quote: Mouse spleens were collected in ice-cold storage solution (PBS 2% FBS) and single-cell suspensions prepared using cell strainers (BD-Falcon). Cells were washed upon red blood cells lysis (150 mM NH4Cl ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Immunology 2019Quote: ... Activation assays were performed by culturing cells with recombinant human IL-2 (10ng/ml, RND Systems) in the presence of coated anti-CD3 and anti-CD28 (2mg/ml, BD Biosciences) in the culture medium ...
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bioRxiv - Immunology 2019Quote: ... were resuspended in D-PBS supplemented with 2% (v/v) FBS containing the antibody cocktail in 96-well microtiter plates (BD Biosciences) for 20 min on ice in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were passed through a 40 μm filter and stained with 2 μg/mL DAPI before sorting on a BD FACSARIA III (BD Biosciences). Aliquots of 40,000 nuclei were collected in 10X binding buffer (200 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected tissue was mechanically triturated by a fire-polished glass pipette and plated onto 24-well plates coated with 2% laminin (BD Biosciences) and 1% poly-D-lysine (Sigma Aldrich) ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Biophysics 2020Quote: ... and a strain with a known binding site and repressor copy number (R) - were inoculated into individual tubes with 2 mL of Lysogeny Broth (LB Miller Powder, BD Medical) with 20 µg/mL of chloramphenicol and 30 µg/mL of kanamycin ...
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bioRxiv - Immunology 2020Quote: ... the cells were diluted in 2% exo-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed twice with PBS containing 2% FBS and were blocked with CD16/32 Fc block (Cat. 553142, BD Bioscience) before the analysis ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The 24-color extracellular staining master mix (Extended Data Table 2) was prepared 2X in BD Horizon™ Brilliant Stain Buffer to prevent staining artifacts from BD Horizon Brilliant dye interactions and added 1:1 to cells ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of 1 μg/mL brefeldin A and monensin (00-4505-51, eBioscience) for 2 hr and were then fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid peroxidation was detected by measuring the fluorescence intensity of BODIPY 581/591 C11 using BD FACS Canto 2 (BD Biosciences). In each case ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing both DOR and dCas9 were isolated using fluorescence activated cell sorting (FACS) on a BD FACS Aria 2 (BD Biosciences), serially diluted ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Cell Biology 2024Quote: The human iPSC line MUSli001-A was generated from skin fibroblasts from a caesarean section [34] and cultured on a polystyrene plate coated with 2 µg/mL Matrigel (Growth Factor Reduced; Corning, BD Bioscience) at 37°C with 5% CO2 ...