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Citations for Becton, Dickinson and Company :
4051 - 4100 of 8448 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Inhibition of CELA1 Improves Septation in the Mouse Hyperoxia Model of Impaired Alveolar DevelopmentbioRxiv - Developmental Biology 2024Quote: One million cells from each lung were incubated (4°C, 30 minutes) with anti–mouse CD16/CD32 (BD Biosciences, 553142) to block Fc receptors ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer’s instructions to exclude dead cells and then labelled in FACS buffer with the following antibodies: V500 Rat anti-Mouse CD4 (RM4-5, BD), eF450 CD5 Monoclonal Antibody (53-7.3 ...
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bioRxiv - Microbiology 2024Quote: ... The suspension was washed twice with PBS and blocked with Mouse BD Fc Block (2.4G2, catalog no. 553141, BD Biosciences) prior to staining ...
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bioRxiv - Immunology 2024Quote: ... or adenosine diphosphate [ADP] 10 μmol/L) by using rat anti-Mouse CD16/CD32 Fc block (BD biosciences, Heidelberg, Germany) and the following purified or conjugated antibodies (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The resulting cell pellet was resuspended in FACS buffer with 0.5 mg/mL rat anti-mouse CD16/CD32 Fc block (Cat. No. 553142, BD Biosciences) and incubated for 15 min on ice to block Fc receptors ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... levels were measured using the Cytometric Bead Array Mouse Inflammation Kit (catalog number 558266, BD Biosciences, Le Pont de Claix), and analyzed with FCAP Array software (3.0 ...
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bioRxiv - Immunology 2024Quote: ... The resulting cell pellet was resuspended in FACS buffer with 0.5 mg/mL rat anti-mouse CD16/CD32 Fc block (Cat. No. 553142, BD Biosciences) and incubated for 15 min on ice to block Fc receptors ...
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bioRxiv - Immunology 2024Quote: ... 2.5×106 lung cells or all recovered BAL cells were incubated with a purified rat IgG2b anti–mouse CD16/CD32 receptor antibody (BD) for 201min at 4°C in FACS buffer (PBS supplemented with 1% BSA and 0.051mM EDTA) ...
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bioRxiv - Immunology 2024Quote: ... 20min at 4°C and then blocked with 100µl of PBS/2mM EDTA + FBS 5% + 2,5 ng/ml purified rat anti-mouse CD16/CD32 (BD Biosciences #553141) 20min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Immunostainings were performed using the following antibodies: rat anti-mouse Mac-3 monoclonal antibodies (550292; BD Pharmingen, San Diego, CA)and Cleaved Caspase-3 rabbit monoclonal antibodies (9664 ...
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bioRxiv - Cancer Biology 2024Quote: ... and leukaemia burden quantified by flow cytometry using GFP and hCD45 (PE-Cy7 mouse anti-human CD45 (BD Pharmingen #557748)) expression ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were incubated with Fc receptor blocking antibody (CD16/CD32 Rat anti-Mouse, Unlabeled, Clone: 2.4G2, BD, fisher scientific) in washing solution (WS;1xPBS+1%FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were incubated with Fc receptor blocking antibody (CD16/CD32 Rat anti-Mouse, Unlabeled, Clone: 2.4G2, BD, fisher scientific) in washing solution (WS;1xPBS+1%FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by BCA assay and 250 µg protein lysates were incubated with anti-Myc (2.5 µg, BD Pharmingen 551102) or anti-IgG (2.5 µg ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. Surface expression level of S proteins (Extended Data Fig. 6a) was measured using FACS Canto II (BD Biosciences) and the data were analysed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Molecular Biology 2020Quote: ... High sensitivity C-reactive protein (CRP) was measured in serum: blood was collected into a silica and gel containing tube (BD Vacutainer).
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then left unstimulated or stimulated with 50 ng/mL PMA and 1uM Ionomycin for 7 hours in the presence of a protein transport inhibitor (BD GolgiPlugTM, BD Biosciences). To stain for intracellular cytokines cells were washed with PBS supplemented with 3% FBS followed by a fixation and permeabilization step with FIX & PERM Kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Green fluorescent protein (GFP) positive cells in HRasL61 with GFP transfected cells were sorted using flow-cytometry (FACS Aria II; BD Bioscience).
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bioRxiv - Physiology 2022Quote: The geometric mean fluorescence intensities (MFI) corresponding to the probes/target protein levels were determined by flow cytometry acquired on Aria II (BD Biosciences) or CytoFLEX (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: PBS-washed cells were PFA-fixed and immunostained for individual surface protein expression using the following antibodies: Anti-CD3-FITC (#561807; BD Biosciences), anti-CD4-APC (#555349 ...
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Immunology 2022Quote: ... and 10% heat-inactivated FBS) and incubated at 37°C for 6 hours with protein transport inhibitors GolgiStop and GolgiPlug (BD Biosciences) and S glycoprotein peptide pools 1 μg/mL (JPT product PM WCPV-S-1 ...
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bioRxiv - Immunology 2020Quote: PBMCs or purified T cells were stained for 30 minutes on ice with the antibodies specific for extracellular proteins in Brilliant Stain Buffer (BD, 563794). Following incubation ...
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bioRxiv - Genetics 2020Quote: ... cells were stained with recombinant biotinylated spike protein RBD as described above and analyzed with multiparametric flow cytometry on a FACS Aria III (BD Biosciences) and FlowJo.v10 software (BD Biosciences).
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bioRxiv - Genetics 2020Quote: ... and the amounts of recombinant ACE2-GFP and dACE2-GFP proteins in lysates were quantified by densitometry analysis (Imagelab, BD Biosciences) of Western blots ...
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bioRxiv - Cell Biology 2021Quote: ... and monoclonal cell lines were generated by isolating cells expressing both TauRD fusion proteins in a 96 well-plate by cell sorting with a BD FACS Aria III (BD Biosciences) following amplification ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Transduced cells were selected with 5μg/ml blasticidin and bulk cells expressing the TetR protein were clonally isolated in 96-well plates using FACS (BD-Aria). BRAFV600E and BRAFWT were expressed in cells using Gateway-compatible lentiviral constructs (pLenti CMV/TO puro DEST ...
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bioRxiv - Microbiology 2021Quote: ... The lung infiltrated cells were cultured with purified RBD protein at 10 μg/ml for 12 h at 37 °C followed by a 6 h treatment with GolgiPlug (BD 555028). After blocking with FcγIII and FcγII receptors antibody (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Immunology 2022Quote: ... Spike and RBD tetramers were prepared fresh before use and formed by combining biotinylated proteins with PE-conjugated Streptavidin (BD Biosciences) at a molar ratio of 4:1.
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bioRxiv - Immunology 2022Quote: ... Protein transport inhibitor containing Monensin and protein transport inhibitor containing Brefeldin A were added to cells (both 10 μg/ml, BD Biosciences) and cells cultured for an additional 4 hours ...
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bioRxiv - Immunology 2024Quote: ... NK cells were either settled on substrates functionalized with NKp46 antibody or incubated with K562 cells in presence of BV421 anti-CD107a antibody and protein transport inhibitor Golgi stop (BD Biosciences) at 37°C with 5% CO2 for 4 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nuclei were resuspended in 500 μl of wash buffer and labelled with an anti-nuclear pore complex proteins antibody (clone Mab414) and sorted on a FACSAria instrument (BD Biosciences), with a 100 nozzle on a cold block ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfected cells were selected with 5 mg/mL puromycin and sorted based on red fluorescent protein (RFP) by fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... we first labeled cell surface proteins in FACS buffer for 30 minutes before washing with 1x PBS and fixation with BD Cytofix/Cytoperm (BD Biosciences) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... was used as a secondary antibody. The surface expression level of S proteins (Fig. 3d) was measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
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bioRxiv - Microbiology 2023Quote: ... was used as a secondary antibody. The surface expression levels of S proteins (Fig. 4A) were measured using a FACS Canto II (BD Biosciences) and the data were analyzed using FlowJo software v10.7.1 (FlowJo LLC) ...
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bioRxiv - Neuroscience 2023Quote: Expression of the different fluorescent proteins (ORFs) of the stable GEFRI2 line were measured by High Throughput LSR Fortessa (BD Biosciences) fluorescence-activated cell sorter (FACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
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bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Transduced pools of cells were sorted by Blue Fluorescent Protein (BFP) expression in BD FACSAria II SORP cell sorter (BD Biosciences). Gene edition was induced in mESC lines by 5-day treatment with 2 μg/ml Doxycycline (Sigma ...
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bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and co-cultured with the T cells for six-hours in the presence of protein transport inhibitor 0.2uL BD GolgiPlug (BD Biosciences 555029), 0.2uL GolgiStop(BD Biosciences 554724) ...
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bioRxiv - Cancer Biology 2024Quote: ... 4:1 CTL:cancer cell admixtures were incubated for 4 h at 37°C in the presence of BD GolgiPlug protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody ...