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Citations for Becton, Dickinson and Company :
4051 - 4100 of 5061 citations for Hum ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... were resuspended in D-PBS supplemented with 2% (v/v) FBS containing the antibody cocktail in 96-well microtiter plates (BD Biosciences) for 20 min on ice in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... 20μg of total protein was loaded per lane and probed with the following primary antibodies dissolved in TBS-T: Topoisomerase IIβ (1:1000, overnight) (BD Biosciences), αTubulin (DMIA ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the cell surface of freshly-prepared epidermal cells was labeled with the following antihuman protein antibodies (clone, manufacturer): FITC–conjugated CD1a (HI149) and PE–conjugated HLA-DR (G46-6, both BD Pharmingen), PE–CF594–conjugated CD3 (UCHT1 ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of the cells was confirmed by staining cells with PE-CD71 and allophycocyanin (APC)-Ter119 antibodies (all from BD Biosciences) by flow cytometry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
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bioRxiv - Cancer Biology 2021Quote: ... 1x105 human EGFR-mutated NSCLC cells plated in 60-mm dishes were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Annexin V antibodies (BD Biosciences) together with propidium iodide (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Kidney sections were blocked (10% normal goat serum and 1% bovine serum albumin (BSA) in PBS) and incubated overnight at 4°C with primary antibody ((Ly6G, 1:10; BD/Pharmingen), FITC-C3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-Human CD44 and mouse anti-Human CD184 antibodies (BD Biosciences, San Jose, CA) at a concentration of 5μL/10 million cells ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with the medium and stained with subsequent antibodies/reagents using the Brilliant Stain Buffer Plus (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cell were washed three times with HF buffer and incubated with secondary antibodies APC-Cy7 goat anti-rabbit IgG (BD, 611203) used at 1:200 dilution and AF647 Donkey anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with 5ul human Fc Block with 95ul 2% FBS in PBS at 4°C for 15 mins before staining with SCMK and AbSeq antibodies (BD Biosciences) at 4°C for 60 min ...
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bioRxiv - Immunology 2021Quote: ... The stained cells were subsequently washed with FACS buffer to remove unbound antibodies and probe and then filtered through 70-μm cell mesh (BD Biosciences) before processed in the FACS sorter ...
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... bone marrow cells were subjected to red blood cell lysis and stained with fluorochrome-conjugated anti-mouse antibodies to CD11b (Mac-1, BD Biosciences) and/or Ly6G (1A8 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary hMPCs were sorted using fluorescence activated cell sorting with fluorescently-conjugated antibodies to cell surface antigens specific to hMPCs [CD56 (NCAM, BD Pharmingen) and CD29 (β1-integrin ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was stained with the corresponding primary antibody-dye conjugates diluted in 100 μl Pharmingen stain buffer (BD Biosciences, #554657). After 1 h staining at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated with detection biotinylated anti-IFNγ antibody for 2h and followed by incubation with streptavidin-horse radish peroxidase (BD Biosciences) for 1h ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences). Alkaline phosphatase substrate was prepared by dissolving SigmaFast (Sigma ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained for surface markers using combinations of the following directly conjugated monoclonal antibodies: anti-CCR7–BV650 (clone 3D12; BD Biosciences), anti-CCR7–PE-Cy7 (clone 3D12 ...
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bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions from mesenteric lymph nodes IL-4 ELISPOT were performed using mouse IL-4 ELISPOT pair antibodies (BD Bioscience) 200,000 cells/well were cultured on multiScreen IP filter plate (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were stained with fluorophore-conjugated antibodies and analyzed on a MACSQuant flow cytometer (Miltenyi Biotech) or sorted using the FACSAria Fusion cell sorter (BD Biosciences). Flow cytometry analysis was based on viable and single-cell gaiting strategies ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions were first stained for viability with LiveDead eFlour 780 stain (eBioscience) and stained with antibodies diluted in Brilliant Stain Buffer (BD Biosciences) or FACS buffer (1% BSA in PBS) ...
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bioRxiv - Immunology 2020Quote: ... for the last 4hrs before subjecting for fluorochrome-conjugated antibody staining for surface markers and intracellular cytokine (Perm/Fix buffer, BD Biosciences). Cells were analyzed by 24-color compatible full-spectrum Cytek Aurora flow analyzer (College of Veterinary Medicine ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... a combination of the following anti-mouse antibodies was used to stain for surface markers (all from BioLegend, eBioscience, or BD Biosciences): anti-CD3ε (145-2C11) ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block and subsequently stained with a combination of the following anti-mouse antibodies (all from BioLegend, eBioscience, or BD Biosciences): anti-CD8α (53-6.7) ...
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bioRxiv - Immunology 2020Quote: ... cells were first stained for viability with LiveDead eFlour 780 stain (eBioscience) for 30 minutes and then stained with antibodies and MHC I tetramers diluted in Brilliant Stain Buffer (BD Biosciences) for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... and BM as previously described (Ciucci et al., 2015) and were stained with different antibodies used for FACS (FACS canto, BD Biosciences) are indicated in the figures.
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescent labelled anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). Flow cytometry analysis was performed using following antibodies ...
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bioRxiv - Immunology 2020Quote: Single-cell suspensions from the intestine or bone marrow were stained with fluorescently labelled antibodies and sorted (purity > 98%) using a BD FACSAria III cell sorter (BD Biosciences). For ILC ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular cytokine staining of cells were stained with following antibodies with maintaining supplier’s instructions: V500 anti-mouse CD45 (BD Bioscience, USA), FITC anti-mouse CD4 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Purified MDSCs from spleen and bone marrow cells were isolated using a mojosort magnet followed by cell surface staining with CD11b and Gr1 monoclonal antibodies for cell sorting on BD FACS Aria III cell sorter (BD Biosciences). Similarly ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were obtained from the spleen of naive mice following antibody labeling and sorted on flow cytometer BD FACS Aria III cell sorter (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... IFNγ-producing cells were stained with IFNγ Detection Antibody in conjunction with cell surface markers (listed below) and analyzed on an LSRFortessa flow cytometer (BD Biosciences). Antibodies used included CD3e (145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibodies for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before analysis using BD Symphony Flow Cytometer ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...