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Citations for Becton, Dickinson and Company :
3951 - 4000 of 5061 citations for Hum ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Post-culture cells were pelleted in V-bottom 96-well plates and resuspended in 30 μl of antibodies at pre-optimized concentrations and diluted in Brilliant Stain Buffer (BD Biosciences), then incubated for 15’ at RT ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a panel of antibodies (Supplementary Table 1) in flow cytometry staining buffer with BD Horizon Brilliant Stain Buffer Plus (BD Biosciences) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... one section out of ten was incubated with BrdU antibodies (BrdU, 1/1,000, CldU, 1/500, Accurate Chemical; IdU, 1/500, BD Bioscience). Bound antibodies were visualized with Cy3-goat anti-rat antibodies (1/1,000 ...
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bioRxiv - Microbiology 2020Quote: ... for 5 min and incubated for 30 min with optimal concentrations of fluorochrome-conjugated antibodies (Table S2) diluted in Brilliant Stain Buffer (BD Biosciences). After three washes with FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hr at 4°C with extracellular domain anti-GluN1 antibody (BD biosciences; 2 μg ml−1) followed by two washes with ice cold phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed twice 5 min in Tris buffer saline containing 0.01% Triton X-100 (TBST) and incubated with the primary antibody over night at 4°C (human and mouse a-Syn: 610786 BD Biosciences). After incubation with the first antibody ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with fluorochrome-conjugated (BV421, BV711, FITC, PE, PE-Cy7, allophycocyanin [APC], APC-Cy7) antibodies against mouse CD45 (30-F11; BD Biosciences), Ly6C (AL-21 ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were then incubated with primary antibodies (ACE2, ab15438, Abcam, UK; HCG beta, ab9582, Abcam, UK; HLA-G, 557577, BD Pharmingen) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Discs were then washed three times for 10 minutes each in 0.1% PBST and incubated overnight at 4°C in mouse anti-BrdU primary antibody (B44) (BD, 1:50). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interest were visualized after incubation with primary antibodies (α-synuclein 1:1000 BD Biosciences (San Jose, CA, USA) #610787 ...
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bioRxiv - Bioengineering 2021Quote: ... at 1:1 E:T ratio at 37°C with 5% CO2 in the presence of a CD107a antibody for 1 hr before treatment with brefeldin A (10 µg/ml; BD Biosciences) and monensin (0.7µg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... An antibody panel for macrophage and T cell polarization was also stained for consisting of: F4/80 BUV395 (T45-2342, BD Biosciences), CD86 BV786 (GL-1 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 72 h at 4°C in mouse anti-PKCδ primary antibody (1:1000, BD Biosciences, 610397) in blocking solution containing 1.5% NGS ...
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... 8.0 × 105 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107) ...
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bioRxiv - Immunology 2021Quote: ... and stained for 30 min on ice with a cocktail of antibodies diluted in a 1:1 mixture of FACS buffer and the Brilliant Stain Buffer (BD Biosciences). The antibody cocktail consisted of APC/Cy7-CD3 (SK7 ...
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bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells from the remaining five brains were labeled with APC-Cy7 rat anti-CD11b antibody (BD Pharmingen, Cat. No. 557657) for 30min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were re-stimulated for another 6 h with the anti-CD3 antibody in the presence of both GolgiStop and GolgiPlug (BD Biosciences) and stained with antibodies against IFN-γ and TNF-α ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with antibodies according to the manufacturer’s specifications and analyzed with a BD FACSCelesta™ Flow Cytometer (BD Biosciences). Engraftment and lineage analysis was determined by staining peripheral blood with CD45.1 (BioLegend Cat#110708 ...
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bioRxiv - Cancer Biology 2021Quote: ... Flow cytometry for negative checkpoint regulator was performed as previously described(14) using antibodies: BV510 mouse anti-human CD279 (PD1) (1:100; 563076, BD Horizon), PerCP-eFluor 710 mouse anti-human CD223 (LAG-3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked with 10% goat serum in PBS with sodium azide at RT for 15 mins before incubating with anti PD-L1 antibody (1:100; clone 6C11-3A11) or isotype rat IgG2a control (1:50; BD Biosciences) at RT for 30 mins21 ...
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bioRxiv - Cancer Biology 2021Quote: ... The levels of DNA-loaded MCM were detected by incubating cells in anti-MCM2 BM28 antibody (1:200, BD biosciences, 610700) for 1 hr at 37°C in the dark followed by incubation in anti-mouse-488 secondary antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were activated in plates coated with anti-mouse CD3ε and anti-mouse CD28 antibodies (1 µg/ml, BD Biosciences) for up to 72h.
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bioRxiv - Cell Biology 2022Quote: ... 100 ug of clarified lysates were diluted to a final concentration of 2 ug/ul and incubated with 60 ul of Dynabeads prebound to 7.5 ug of either anti-Cdk1 Antibody (BD Biosciences - 610037) or Mouse Ig-G ...
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bioRxiv - Cancer Biology 2022Quote: ... were blocked for overnight in 5% goat serum 0.3% Triton in PBS and incubated with 10 µL/mL Alexa 647-conjugated anti-BrdU antibody (BD biosciences, 560209) or anti-cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... The newly replicated CldU and IdU tracts were incubated with anti-BrdU antibodies recognizing CldU and IdU respectively (ab6326, Abcam; 347580, BD Biosciences) for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Cell Biology 2022Quote: Bone marrow cells from mice were labeled with a cocktail of biotin-conjugated lineage marker antibodies in combination with Streptavidin Particles Plus (BD, 557812) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: PBS-washed cells were PFA-fixed and immunostained for individual surface protein expression using the following antibodies: Anti-CD3-FITC (#561807; BD Biosciences), anti-CD4-APC (#555349 ...
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bioRxiv - Immunology 2022Quote: ... ex vivo-stimulated cells were stained with cell surface markers followed by staining with anti-cleaved caspase-3 antibody (C92-605; BD Biosciences) for 45 minutes at 4°C after processing with Fixation/Permeabilization kit (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents: PE-conjugated rat anti-mouse Siglec-F (E50-2440; BD Biosciences), PE-TxR-conjugated rat anti-mouse CD11b (M1/70.15 ...
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bioRxiv - Cancer Biology 2022Quote: About 2×105 cells per sample were blocked using Purified NA/LE Rat Anti-mouse CD16/CD32 Clone 2.4G2 antibody (BD Biosciences, #553140) followed by incubation with 1 μl of specific antibodies for 30 min on ice in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then cultured on anti-hamster IgG coated plates in the presence of hamster anti-CD3 epsilon and anti-CD28 antibodies (BD Biosciences), neutralizing anti-IL-4 antibody (eBioscience) ...
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bioRxiv - Cancer Biology 2022Quote: ... barcoded and permeabilized as previously described.31 The samples were stained with the indicated antibodies and analyzed with a BD LSR Fortessa cytometer (BD Biosciences) equipped with 488 nm ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used herein at 1:200 dilution unless otherwise indicated: Fixed viability stain-700 (FVS700, BD, 1:1000), anti-IL-17A (TC11-18H10 ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... for 20 minutes and then with a blood antibody cocktail for 30 minutes in the dark at 4 degrees: anti-CD45 BB515 (BD Horizon), anti-CD14 PerCP-Cyanine5.5 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... mR and mL were determined by comparing mean fluorescence intensity (MFI) of targeted proteins stained by saturating amount of fluorescently-tagged antibody to MFI of PE quantitation beads (BD Biosciences). Ac and tc are controlled parameters during experiments.
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bioRxiv - Cancer Biology 2022Quote: ... or Jurkat (CD19-) cells were stained with a phycoerythrin (PE)-labelled anti-CD47 (B6H12 clone) or isotype control antibody (BD Biosciences). The cells were pre-treated for 1 h with TG-1801 or an anti-human CD47 (B6H12 clone ...
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bioRxiv - Neuroscience 2021Quote: ... and their Fc receptors were blocked for 10 min at 4 °C with anti-CD16/CD32 antibodies (10 μg/mL: BD Biosciences). After blocking ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with fluorochrome-conjugated antibodies for 30min at 4°C in the dark in brilliant stain buffer (BD Biosciences). Prior to fixation and permeabilization for intracellular staining with Cytofix/Cytoperm (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... The cells were incubated for one hour at 4°C with a cocktail of fluorochrome-labelled antibodies consisting of anti-CD3-BV510 (cat. no: 563024, BD Biosciences), anti-B220-APC-Cy7 (cat ...
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bioRxiv - Cell Biology 2019Quote: ... blocked for 1 hr in 1% BSA in TBS and then incubated for 1 hr with mouse anti-NF-KB p65 antibody (BD biosciences) at 1:1000 dilution in 1% BSA ...
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bioRxiv - Immunology 2019Quote: ... Cells were initially stained with fluorochrome-conjugated antibodies against surface receptors (see Table S1) in BD Brilliant stain buffer (BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... dissected tissues were then incubated overnight with rabbit anti-Afg3l2 (1:250, Antibody plus CSB-PA04005A0Rb) and mouse anti-cox4 (1:1000, BD Biosciences) antisera ...
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bioRxiv - Microbiology 2021Quote: Nectin-1 was detected on the surface of cells by staining them with anti-nectin-1 antibody CK41 conjugated to PE (BD Biosciences). HVEM was detected on the surface of cells using the anti-HVEM polyclonal antibody ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated in PGB for 25 min with antibodies against Lewis-X (1:20, LeX/CD15 FITC, MMA; BD Bioscience), CD133-APC (1:50,13A4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...