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Citations for Becton, Dickinson and Company :
3751 - 3800 of 3828 citations for Podocalyxin PODXL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The following primary antibodies were used: AF-488 anti-rat CD3 (Biolegend. Clone 1F4, PE-Cy5 Mouse anti-rat CD4 (BD bioscience. Clone Ox-35), PE Mouse anti-rat CD8α (BD bioscience ...
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bioRxiv - Bioengineering 2021Quote: ... cells were stained with surface marker antibodies and analyzed with Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Propidium Iodide (5:1000 µL ...
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bioRxiv - Cancer Biology 2019Quote: Glioma TMA sections (4 μm) were stained using a rat monoclonal primary antibody to the integrin β4 subunit (CD104; clone 439-9B; BD Pharmingen, San Jose, CA) at a concentration of 5μg/ml according to a previously described protocol (11) ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... cells were centrifuged and resulting pellets were resuspended in 1:100 mixture of panel of antibodies: FITC-conjugated CD31 (BD Biosciences, Mississauga, ON, Canada), AF647-conjugated CD45 (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... A 3% BSA-0.2% Triton®X-100 solution was used for permeabilization and blocking followed by a 1 h RT incubation with PBS–1% BSA containing mouse monoclonal DRP1 antibody (611113, BD Transduction Laboratories, 1:50) followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121 ...
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bioRxiv - Cancer Biology 2021Quote: ... Negative cells were sorted for the absence of PD-L1 by staining with a PE-labeled anti-mouse PD-L1 antibody (clone MIH5, BD Biosciences, Franklin Lakes, NJ) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were FACS sorted four times for expression of CD40L using an anti-CD40L antibody (clone TRAP1, BD Bioscience, San Jose, California). This resulted in a stable CD40L-expressing cell line that was cultivated in G418 containing selection media to maintain expression ...
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bioRxiv - Immunology 2020Quote: ... LN staining: macrophages were detected using a primary antibody against CD11b (1/1000, rat anti mouse CD11b conjugated with PE; BD Biosciences, San Jose, California), B-cell follicles were identified using rat anti mouse B220 Alexa F647 (1/1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were blocked in 5% BSA and probed overnight at 4°C with the following primary antibodies and dilutions: p16 (1:200, #554079 BD Biosciences, San Jose, CA), p53 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in 100 μl of staining solution of FACS buffer containing biotinylated antibodies against CD31 (BD Biosciences, 558737, 1:100), CD45 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were washed 5x times with TBS-T and stained overnight at 4°C with gentle agitation with primary antibodies: mouse anti-rat CD63 (1:200 dilution, BD Biosciences, Mississauga, ON, Canada), rabbit anti-rat CD9 (1:500 dilution ...
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bioRxiv - Genomics 2022Quote: The collected cells were first stained with live/dead aqua to exclude non-viable cells from the analysis and stained with fluorophore-conjugated antibodies against surface markers anti-CD45-BV786 (BD Bioscience Clone: OX-1), anti-CD11b-V450(BD Bioscience Clone ...
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bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with primary antibodies for at least 1 hr at room temperature and secondary antibodies (for nonconjugated primary antibodies) for 30 min at room temperature with washes between the steps before analysis by flow cytometry (BD Fortessa or MACSQuant 16). To exclude dead cells ...
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bioRxiv - Cell Biology 2024Quote: ... upper membrane parts were first probed with rat monoclonal anti-pSMAD1/9 antibody (pS463/pS465, pS465/pS467, 1:1,000, #562508, BD Biosciences, East Rutherford, NJ, USA), or rabbit monoclonal anti-pSMAD2 (S465/467 ...
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bioRxiv - Developmental Biology 2024Quote: ... Animal caps were blocked for 1 hour using sheep serum and bovine serum albumin and then incubated in 1:250 anti-β-catenin antibody (BD Bioscience cat# 610154) in blocking solution overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The samples included for T cell phenotypic characterization were stained with a separate antibody cocktail containing T cell lineage markers (CD3-BV786 (BD, cat. #563799, clone SK7), CD4-BV650 (BD ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed with PBS+FBS and incubated with the monoclonal antibody anti-mouse CD8 T conjugated to Fluorescein Isothiocyanate (FITC) (BD-Pharmingen, San Diego, CA), which was suitable for determining the phenotype of CD8+ T cells ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... 1x106 cells in 100 μL were stained for 30 minutes at room temperature using the following antibodies: CD90-BUV395 (BD, #740260, 0.1 mg/μL), ECAD-AF405 (Novus Bio ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluoresceinated antibodies against (TCRý, CD4, CD8, CD3, CD3χ, CXCR5, PD1, and intracellularly for ZAP70 and IL-10) (BD Biosciences, San Jose, CA) and data acquired on a FORTESSA flow cytometer and analyzed using FlowJo Software (Ashland ...
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bioRxiv - Genetics 2023Quote: ... USA)/anti-rabbit IgG Alexa 488 (Jackson Immuno Research lab, West Grove, PA, USA) and 10µg/mL biotinylated anti-CD45 antibodies (BD Bioscience, Franklin Lakes, NJ, USA)/anti Rat IgG Alexa Plus 647 (Thermo Fisher Scientific ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: pCAF sorting based on TNFRSF12A protein level: 106 cells were incubated with BD Horizon BV421 anti-TNFRSF12A antibody (1/140, 565712 BD Biosciences, Franklin Lakes, NJ) and human TruStain FcXTM (1/200 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4°C with primary antibodies: ALIX (BD Biosciences, 611620, 1:1000), calnexin (Santa Cruz ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in TBS/Tw and then incubated overnight with primary antibody Syn-1 (BD Biosciences #610,787, 1:1000) or MJFR-14-6-4-2 (Abcam #209,538 ...
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bioRxiv - Microbiology 2023Quote: Surface markers of splenocytes were identified using monoclonal antibodies in conjunction with two immunofluorescence analysis with a flow cytometer (BD Accuri C6 Plus: BD, Mountain View, CA, USA). Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1 ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes were cultured in RPMI supplemented with 10% FBS and 50 μM beta-mercaptoethanol (RPMI media) Lymphoblasts were generated by activation with plate bound murine anti-CD3ε antibody (BD Biosciences, Clone: 145-2C11) in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed with phosphate buffer three times for 10 minutes each and incubated with primary antibody (rat anti-mouse CD31, BD Biosciences, 550274, 1:100) overnight at 4°C ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin in PBS for 45 min at 37°C was followed by incubation with a primary antibody mix of anti-BrdU detecting IdU (mouse, #347580, BD, Franklin Lakes, NJ, USA) and anti-BrdU detecting CldU (rat ...
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bioRxiv - Immunology 2024Quote: ... according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163 ...
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bioRxiv - Immunology 2024Quote: ... was prepared by coating the well with 50 μl of 10 μg/ml of anti-mouse IFNγ monoclonal capture antibody (BD Biosciences; cat. no. 551309) in PBS and allowed to incubate at 4°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... The ELISPOT plate was washed five times with PBST and 50 μl of 1 μg/ml of the anti-mouse IFNγ monoclonal detection antibody conjugated to biotin (BD Biosciences; cat. no. 551506) in PBS was added and allowed to incubate at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature followed by overnight incubation at 37°C in antibodies to the following: (1) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences, used at 1:200). (2 ...
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bioRxiv - Microbiology 2021Quote: ... sections were incubated for 30 minutes at room temperature with a rat monoclonal antibody anti-Mouse CD8 (BD Pharmingen, BD Bioscience, San Jose, CA, USA) diluted 1:50 in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... and the cell suspensions purity was higher than 90% as assessed by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were washed three times with PBS and CD34+ cells were isolated as described 35 using rhesus macaque-specific antibodies to CD34 (clone 563; BD Pharmingen, San Jose, CA, USA) and the anti-PE MACS magnetic bead system (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... The fluorescent signal from GLI1 OE and control cell samples stained for cTnT-positivity was normalized to fluorescent signal from cells stained with a BV421-conjugated isotype control IgG antibody (BD Biosciences 562438, Lot 8242926, X40). Fluorescence was quantified ...
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bioRxiv - Immunology 2020Quote: ... Single cells were stained with the different fluorescent antibodies (as indicated in the figure legends) and DsRed on the BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA). Analyses were completed using FlowJo analysis software (FlowJo ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained for 30 min at 4°C under darkened conditions with the following fluorochrome labeled monoclonal antibodies: anti-CD4-AmCyan (Clone SK3, BD Biosciences, Franklin Lakes, Nj, USA), anti-CD25-APCCy7 (Clone M-A251 ...