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Citations for Becton, Dickinson and Company :
3351 - 3400 of 3828 citations for Podocalyxin PODXL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Immunology 2021Quote: ... The stained cells were subsequently washed with FACS buffer to remove unbound antibodies and probe and then filtered through 70-μm cell mesh (BD Biosciences) before processed in the FACS sorter ...
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bioRxiv - Immunology 2021Quote: ... cryo-preserved PBMCs were first thawed at 37°C in a water bath and washed with an RPMI medium containing 10% fetal bovine sera (FBS) following incubation with fluorescently-labeled antibodies (BD Biosciences) against cell surface markers for CD3 (PE-Cy7) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse femur single cells were obtained as described above in the flow cytometry section and stained with biotin-conjugated antibodies for Lin (Ter119, Gr-1, B220, CD3e) (BD, 559971) followed by negative selection using streptavidin-coated magnet beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... bone marrow cells were subjected to red blood cell lysis and stained with fluorochrome-conjugated anti-mouse antibodies to CD11b (Mac-1, BD Biosciences) and/or Ly6G (1A8 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary hMPCs were sorted using fluorescence activated cell sorting with fluorescently-conjugated antibodies to cell surface antigens specific to hMPCs [CD56 (NCAM, BD Pharmingen) and CD29 (β1-integrin ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Synapsin (1:500) and anti-CD133 (1:500) were used after Fc blocking with CD16/32 antibody (553141; BD Pharmingen). Respective Secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was stained with the corresponding primary antibody-dye conjugates diluted in 100 μl Pharmingen stain buffer (BD Biosciences, #554657). After 1 h staining at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated with detection biotinylated anti-IFNγ antibody for 2h and followed by incubation with streptavidin-horse radish peroxidase (BD Biosciences) for 1h ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Immunology 2020Quote: ... The cells (2-4 × 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibodies to either IFN-γ or IL-4 (BD), and were exposed to various stimuli (e.g ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences). Alkaline phosphatase substrate was prepared by dissolving SigmaFast (Sigma ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained for surface markers using combinations of the following directly conjugated monoclonal antibodies: anti-CCR7–BV650 (clone 3D12; BD Biosciences), anti-CCR7–PE-Cy7 (clone 3D12 ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Immunology 2021Quote: ... and cell surface markers were stained with the following anti-mouse antibodies at 1:200 dilution per 106 cells: Ly6C (AL-21; BD Biosciences), Ly6G (1A8 ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions from mesenteric lymph nodes IL-4 ELISPOT were performed using mouse IL-4 ELISPOT pair antibodies (BD Bioscience) 200,000 cells/well were cultured on multiScreen IP filter plate (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Sections were stained using the following antibodies: rat anti-mouse Ly-6C/Ly-6G (RB6-8C5, BD Bioscience, #550291, 1/200) followed by AlexaFluor647-conjugated goat anti-rat IgG (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were stained with fluorophore-conjugated antibodies and analyzed on a MACSQuant flow cytometer (Miltenyi Biotech) or sorted using the FACSAria Fusion cell sorter (BD Biosciences). Flow cytometry analysis was based on viable and single-cell gaiting strategies ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions were first stained for viability with LiveDead eFlour 780 stain (eBioscience) and stained with antibodies diluted in Brilliant Stain Buffer (BD Biosciences) or FACS buffer (1% BSA in PBS) ...
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bioRxiv - Immunology 2020Quote: ... for the last 4hrs before subjecting for fluorochrome-conjugated antibody staining for surface markers and intracellular cytokine (Perm/Fix buffer, BD Biosciences). Cells were analyzed by 24-color compatible full-spectrum Cytek Aurora flow analyzer (College of Veterinary Medicine ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... a combination of the following anti-mouse antibodies was used to stain for surface markers (all from BioLegend, eBioscience, or BD Biosciences): anti-CD3ε (145-2C11) ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block and subsequently stained with a combination of the following anti-mouse antibodies (all from BioLegend, eBioscience, or BD Biosciences): anti-CD8α (53-6.7) ...
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bioRxiv - Immunology 2020Quote: ... cells were first stained for viability with LiveDead eFlour 780 stain (eBioscience) for 30 minutes and then stained with antibodies and MHC I tetramers diluted in Brilliant Stain Buffer (BD Biosciences) for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... and BM as previously described (Ciucci et al., 2015) and were stained with different antibodies used for FACS (FACS canto, BD Biosciences) are indicated in the figures.
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescent labelled anti-mouse antibodies after blocking CD16/CD32 with Fc Block (BD Biosciences, San Jose, CA). Flow cytometry analysis was performed using following antibodies ...
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bioRxiv - Immunology 2020Quote: Single-cell suspensions from the intestine or bone marrow were stained with fluorescently labelled antibodies and sorted (purity > 98%) using a BD FACSAria III cell sorter (BD Biosciences). For ILC ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular cytokine staining of cells were stained with following antibodies with maintaining supplier’s instructions: V500 anti-mouse CD45 (BD Bioscience, USA), FITC anti-mouse CD4 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Purified MDSCs from spleen and bone marrow cells were isolated using a mojosort magnet followed by cell surface staining with CD11b and Gr1 monoclonal antibodies for cell sorting on BD FACS Aria III cell sorter (BD Biosciences). Similarly ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were obtained from the spleen of naive mice following antibody labeling and sorted on flow cytometer BD FACS Aria III cell sorter (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... IFNγ-producing cells were stained with IFNγ Detection Antibody in conjunction with cell surface markers (listed below) and analyzed on an LSRFortessa flow cytometer (BD Biosciences). Antibodies used included CD3e (145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibodies for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before analysis using BD Symphony Flow Cytometer ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Immunology 2021Quote: ... Parasites were then washed with FACS buffer and placed on ice for incubation with anti-isotype detection antibodies depending on application: anti-IgG3-BV421 (R40-82, BD Bioscience), anti-IgM-PE/Cy7 (RMM-1 ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated and immunolabeled with anti-CD4 and anti-Foxp3 antibodies and BrdU incorporation was analyzed by a BrdU Flow kit per the manufacturer’s protocol (BD Pharmingen, 552598) (15).
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-T-bet-BV785 (4B10, BD Biosciences), anti-EOMES-PerCP-ef710 (X4-83 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with a cocktail of antibodies (P1, P2, P3 and P4) in Cell Staining Buffer plus BD Horizon Brilliant Stain Buffer Plus (BD, 566385) (Key Resources Table ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed with fixation buffer and then permeabilized with Cytofix / Cytoperm (e-bioscience) followed by staining for 60 minutes with antibodies that were diluted in 1X perm buffer (BD, 554723). Flow cytometry data acquisition was performed either on LSR Fortessa X-20 (BD ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cell Biology 2022Quote: ... Phenotypic analysis was performed with the following antibodies: Rat anti-mouse active β1-integrin (active CD29 Clone 9EG7, 553715; BD Biosciences); Alexa647-Armenian hamster anti-mouse β1-integrin (total β1-integrin 102214 ...