Labshake search
Citations for Becton, Dickinson and Company :
3001 - 3050 of 3199 citations for 4 nitro 5 ethyl 2 methylpyridine n oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Samples for flow cytometry were fixed in 2% paraformaldehyde solution in PBS and analyzed using a LSRII flow cytometer (BD Biosciences) and FlowJo software (Tree Star ...
-
bioRxiv - Immunology 2023Quote: ... followed by staining with a cocktail of mAbs and four fluorochrome-conjugated spike proteins in staining buffer (2% FBS/PBS) supplemented with Brilliant Stain Buffer Plus (BD Biosciences). The stained cells were fixed (Lysing Solution ...
-
bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
-
bioRxiv - Bioengineering 2023Quote: ... TCR expressing cells were mixed with K562-A2 cells at a ratio of 1:2 (Effector:Target) in the RPMI media and supplemented with 1 µg/ml of anti-CD28/CD49d antibodies (BD Biosciences, 347690) and 1 µg/ml of cognate peptides ...
-
bioRxiv - Biophysics 2022Quote: ... Saccharomyces cerevisiae strain W303-GAL4 was grown overnight in YPDA medium (yeast extract 1 %, adenine sulfate 540 μM, peptone 2 % (BD Biosciences), glucose 2 % ...
-
bioRxiv - Cancer Biology 2023Quote: C4-2 cells were cultured and lifted from plates by trypsinization then re-suspended in 100 mL of 50% Matrigel (BD Biosciences) and saline mixture followed by subcutaneous injection in two separate groups of castrated and non-castrated athymic nude mice (age at least 6 weeks old) ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
-
bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
-
bioRxiv - Immunology 2022Quote: ... cells were fixed with 2% paraformaldehyde for 20 min and resuspended in 350 ul 1% BSA/PBS for phenotyping by FACSDivacytometer (BD Biosciences). To set up the compensation control ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... and the second model investigated ii) four exposures from the first eating event (AK-20, BD, KB, LT & NH; Model 2). In each model ...
-
bioRxiv - Cell Biology 2024Quote: ... IL-6 and IL-1β cytokines were measured in the cell culture supernatant after 2 h of co-exposure using CBA human Inflammatory cytokine kit (BD Biosciences), while chemokines CXCL8/IL-8 ...
-
bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
-
bioRxiv - Immunology 2023Quote: ... The proteases were inactivated by adding 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience). Single cells were then washed with 1% Bovine serum albumin (BSA ...
-
bioRxiv - Cancer Biology 2023Quote: ... or FAK-KD cell lines were generated by injecting a total of 2×106 MDA-MB-231/Dendra2 cells resuspended in 20% collagen I (BD Biosciences) in PBS into the lower left mammary gland of SCID mice ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were suspended in 350 µL of FACS buffer (1XDPBS, 2% FBS, 25mM HEPES, 2mM EDTA) and was analysed by flow cytometry BD LSRFortessa (BD Biosciences). 10,000 cells were analysed for each condition ...
-
bioRxiv - Cell Biology 2023Quote: ... with a purity >95% into sterile filtered PBS with 2% FBS or acquired on a FACS Canto II or LSR fortes Analyzer (BD Biosciences). Single cells were index sorted using single cell depositor ...
-
bioRxiv - Genomics 2023Quote: ... at least 2 × 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
-
bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
-
bioRxiv - Immunology 2023Quote: ... Antibody cocktails for surface proteins were prepared in PBS + 2% FBS and Brilliant Stain Buffer (50 µl/100 µl of cocktail, BD Biosciences) and added to the cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... Anaerobic bacteria colonies were counted after incubating these agar plates for 2 days using the GasPak anaerobic gas generating pouch system (BD Biosciences).
-
bioRxiv - Bioengineering 2023Quote: ... cerevisiae strains were derived from CEN.PK113-7D and grown at 30°C in Yeast Extract Phytone Dextrose media (YEPhD, 2% Difco™ phytone peptone (Becton-Dickinson (BD), Franklin Lakes ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Isolates from the CNRMA in France were subcultured on 2% malt extract agar (Oxoid) and potato dextrose agar (BD Diagnostic Systems) for 5 days at 30 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... was added to a final concentration of 2 μg/mL and cells examined by flow cytometry using a FACSLyric flow cytometer (BD Biosciences). For controls (negative ...
-
bioRxiv - Immunology 2024Quote: T cells were restimulated with 3 μg/ml plate-bound anti-CD3 and 2 μg/ml soluble anti-CD28 or PMA/Ionomycin in the presence of GolgiPlug protein transport inhibitor (BD Biosciences) for 6 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... for viability assessment and fixed with 2% paraformaldehyde (30 min incubation) until analysis by flow cytometry using an LSR Fortessa (BD Biosciences). For assessment of NP-associated fluorescence in a fluorescence plate reader ...
-
bioRxiv - Immunology 2024Quote: ... Cell culture supernatants were assayed for IL-2 using capture and biotin-labeled detection antibody pairs (BD Biosciences, Franklin Lakes, NJ). Detection was performed with HRP-conjugated streptavidin (BD Biosciences ...
-
bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated with phycoerythrin (PE)-conjugated Enpep antibody at 4°C for 60 min and then sorted by FACScan flow cytometry (Becton, Dickinson and company, Franklin Lakes, NJ).
-
bioRxiv - Immunology 2020Quote: ... Cells were then surface stained for 30 min in the dark at 4°C with viability reagent (BD Horizon™ Fixable Viability Stain, BD Biosciences, San Diego, CA, USA) and a 29-color cocktail of monoclonal antibodies (mAbs ...
-
bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
-
bioRxiv - Neuroscience 2019Quote: ... Intracellular cytokine staining was performed according to the manufacturer’s protocol (Cytofix/Cytoperm buffer set from BD with PE-Cy7-conjugated anti-CD4 (RM4-5), eFluor 450-conjugated anti-TCR-β (H57-597) ...
-
bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
-
bioRxiv - Immunology 2021Quote: ... Single leucocyte suspensions were stimulated with either phorbol 12-myristate 13-acetate (PMA)-ionomycin or 5 µg/ml of MCMV peptide pool (Table S3) for 6 hours with brefeldin A (BD Biosciences, San Jose, CA). After stimulation ...
-
bioRxiv - Neuroscience 2020Quote: ... infected mouse brain for flow cytometry were obtained as described.(Posel et al., 2016) Isolated cells were incubated overnight (5 hours for microglia analysis) in GolgiPlug and GolgiStop (BD Bioscience #555029 and #554724) in RPMI media containing HEPES and 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were resuspended in PBS + 5% FBS and analyzed via Fluorescence-Assisted Cytometry (FACs) using a BD LSR Fortessa (BD Biosciences, San Jose, CA). Fluorescence minus one (FMO ...
-
bioRxiv - Microbiology 2021Quote: Lorikeet intestinal contents collected at necropsy were plated on reduced Trypticase Soy Agar (TSA II) with 5% sheep blood (BD BBL, Franklin Lakes, NJ) and incubated anaerobically (5% CO2 ...
-
bioRxiv - Immunology 2020Quote: Single cell preparations were cultured in complete media (106 cells /ml) for 72h in anti-CD3/anti-CD28 (5 μg/mL; BD Biosciences, San Jose, USA) pre-coated 96-flat-well plates (BD Biosciences) ...
-
bioRxiv - Cell Biology 2019Quote: ... The following day they were probed with antibodies to FLAP (Novus, IMG 3160, 1:100) and to 5-LO (BD Biosciences, 610695, RRID: AB_398018). Secondary antibodies were applied at 3 µg/mL for 1 h ...
-
bioRxiv - Genomics 2020Quote: ... at a concentration of 100μg/ml starting 5 days after transfection for the following 7-9 days before single cell sorted using BD FACSAria II (BD Bioscience, San Jose, CA) for clonal cell line generation ...
-
bioRxiv - Immunology 2019Quote: RAMOS cells were resuspended in cR10 media at 5 × 105 cell/mL at a final volume of 10 mL in T25 tissue culture flasks (BD Biosciences, San Diego, CA) and either left untreated or cyclosporine A (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
-
bioRxiv - Microbiology 2021Quote: ... This was done by treating both the PDMS and glass surface with 5-10 sweeps of a portable laboratory corona device (model BD-20ACV, Electro-Technic Products). After treatment the chip was placed carefully onto the glass slide and gently tapped to facilitate full contact between the PDMS and glass surface ...
-
bioRxiv - Immunology 2021Quote: ... The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A) (BD Biosciences, San Jose, CA) at a final concentration of 1:1000 ...
-
bioRxiv - Immunology 2022Quote: ... the frozen bacterial stock was thawed in 37°C water bath and cultured at 37°C in 5% CO2 in Todd Hewitt broth (BD Biosciences, San Jose, CA) to mid-logarithmic phase ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were washed with ice-cold DPBS plus 5 mM EDTA and stained with annexin V and PI (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at RT ...
-
bioRxiv - Immunology 2022Quote: ... 1.5 µg/mL) [31] in the presence of anti-CD28 and anti-CD49d antibodies (both at 1 μg/ml, BD, Franklin Lakes, New Jersey) and brefeldin-A (10 μg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... namely anti-CD3-APC (1:5 dilution, clone SK7) and anti-CD45-PerCP (1:10 dilution, clone 2D1) (both from BD Biosciences, San Jose, CA). After incubation for 30 min at 4°C in the dark ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then stained with monoclonal antibodies conjugated to fluorescent molecules as listed in Supplemental Table 5 after treatment with Fc Block (BD Biosciences, Franklin Lakes, NJ). Cells were analyzed on either a BD LSR-II or BD LSRFortessa X-20 flow cytometer running BD FACSDiva software or a Cytek Aurora spectral cytometer running SpectroFlo software and analyzed using FlowJo software (TreeStar).
-
bioRxiv - Bioengineering 2023Quote: Splenocytes from 125Tg B6 and WT B6 control mice were stained with CellTrace Violet and cultured for 3 days35 in complete RPMI alone or stimulated with 5 ug/mL anti-mouse anti-CD40 (BD Pharmingen clone HM40-3) in the presence or absence of conjugates described above at 150 nM concentrations ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells filtered into 5 ml polystyrene tubes and analyzed by flow cytometry using a FACS Caliber flow cytometer and FlowJo software (BD Biosciences, Franklin Lakes, NJ).