Labshake search
Citations for Becton, Dickinson and Company :
2701 - 2750 of 3199 citations for 4 nitro 5 ethyl 2 methylpyridine n oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Bacteria from cryo stock were resuspended in Brain Heart Infusion (BHI) medium and put onto blood agar plates (Columbia agar with 5 % sheep blood, BD, Cat: 4354005). Plates were incubated in a chamber with anaerobic conditions (83 % N2 ...
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bioRxiv - Immunology 2020Quote: ... Cellular depletion in spleens and intraepithelial lymphocyte compartments was monitored by flow cytometric detection with α-CD4 BV650 (clone RM4-5, BD Biosciences) or α-CD8β (clone H35-17.2 ...
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bioRxiv - Bioengineering 2020Quote: Seven days after electroporation a total of 5 × 106 T cells were collected and stained in 100 uL with fluorophore-conjugated anti-human antibodies for CD3 (BD Biosciences #564001), beta-2-microglobulin (BioLegend #316306 ...
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bioRxiv - Microbiology 2022Quote: ... jejuni on MH agars were resuspended in 5 ml of MH broth in a 14 ml round bottom tube (BD Falcon, USA) to an optical density at 600 nm (OD600 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies (Claudin-5, Abcam Ab131259, 1:1000; Collagen IV, Abcam Ab6586, 1:500; Hemoglobin, R&D Systems G-134-C, 1:300; P62, BD Biosciences 610833 ...
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bioRxiv - Cancer Biology 2019Quote: 5×105 human CD14+ cells were isolated from peripheral blood mononuclear cells (PBMC) using anti-CD14 beads (BD Biosciences, San Jose, CA) with a typical yield of ≥ 70% recovery and ≥ 90% purity ...
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bioRxiv - Bioengineering 2019Quote: ... double emulsions were diluted 1:5 in FACS diluent buffer in a 12 × 75 mm round bottom FACS tube (BD Bio-sciences). For a typical run ...
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bioRxiv - Cancer Biology 2020Quote: ... 5×104 were added to the top chamber of 24-well Boyden chambers (8 μm pore size membrane; BD Bioscience, Bedford, MA), and medium was added to the bottom chambers and incubated for 24 hours (DMEM 10% FBS) ...
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bioRxiv - Microbiology 2020Quote: ... cells were restimulated for 5 hours with the same peptide pool in the presence of brefeldin A (BD Biosciences, San Diego, CA). Cells were then tested for IFN-γ ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then blocked with 5% non-fat milk and probed with the following antibodies: β-catenin (1: 3,000, BD Biosciences, USA), Wnt6 (1 ...
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bioRxiv - Microbiology 2021Quote: ... for in vitro experiments and LB agar (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride, 1.2% agar; BD, San Jose, CA) supplemented with 750 μg/ml ampicillin (EMD Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody dilutions were prepared as follows in 5% NGS in 1X PBST: rat anti mouse CD11b clone M1-70 (BD Pharmingen; #553308) – 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The following antiboodies were used in combination for the depletion of lineage-positive cells: anti-CD4 (RM4-5, BD Biosciences, 1:100), -CD8 (53-6.72 ...
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bioRxiv - Neuroscience 2021Quote: ... and 5 mM EDTA in DPBS and kept cool before undergoing fluorescent activated cell sorting on a FACS Aria™ III (BD Biosciences). Dead cells were excluded from the sort and microglia were selected using CD11b/CD45 double staining and GFP as a marker of infection ...
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bioRxiv - Microbiology 2020Quote: RNA and virus injections of 5 and 10 μl were performed with insulin syringes (BD Micro Fine Plus, 1 ml, 30 G) into honey bee and bumble bee pupae respectively ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug™, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 monoclonal antibody (mAb ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and centrifuged at 1000rpm for 5-10min and stained with APC-Cy™7 Rat Anti-Mouse CD3 (clone: 17A2, Cat # 560590, BD Biosciences), FITC Rat Anti-Mouse CD4 (Clone ...
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bioRxiv - Physiology 2020Quote: ... Microtubules were allowed to grow from SVP in DMEM-F12+10% FBS for 5 days before staining with an anti-CD31 antibody (BD Biosciences, 557355). Images of microtubule outgrow from SVP were acquired with a Leica SP5-SMD confocal microscope ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellet was suspended with FACS medium and incubated with 5 μl of Alexa Fluor 488-conjugated anti-alkaline phosphatase (BD Pharmingen, 561495) and 5 μl of APC-conjugated anti-c-KIT (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Immunology 2022Quote: ... samples were measured by a MACSQuant 18 (Figure 5) or 16 (Supplementary Figure 6) flow cytometer and analysed by FlowJo® software (BD company). Three spleen samples from lmon_0149 vaccinated mice (Figure 5 ...
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bioRxiv - Microbiology 2022Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, NJ, USA). Unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... for 10 min at 37 °C and 5 % CO2 before they were stained with Fixable Viability Stain 510 (1:100, #564406, BD Horizon, USA) for 15 min at room temperature (RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and Parabacteroides distasonis were grown anaerobically at 37°C in brain heart infusion (BHI) media (Oxoid, UK) supplemented with hemin (5 mg/L) and vitamin K (0.5 mg/L) (BD, Franklin Lakes, NJ). Lactobacillus johnsonii and Ligilactobacillus murinus were grown anaerobically at 37°C in de Man Rogosa Sharpe (MRS ...
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bioRxiv - Microbiology 2023Quote: BALF samples were centrifugated at 300 x g for 5 min and the pellets were resuspended in 500 μl Pharm Lyse Buffer (BD Biosciences, 555899) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: BALF samples were centrifugated at 300 x g for 5 min and incubated in 500 μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Bioengineering 2023Quote: ... at 10 × 106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences, US) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Immunology 2023Quote: The proliferation of PBT was induced via CD3 antibody (20 ng/ml, clone: OKT3) and CD28 antibody (5 µg/ml, BD Biosciences 555725) bound to the surface of a 96-well Nunc MaxiSorpTM microtiter plate ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was done by standard techniques following 5-hour stimulation with Gp61-80 peptide and Brefeldin A (GolgiPlug™, BD Biosciences). No peptide controls were treated under the same conditions supplemented with Brefeldin A but without Gp61-80 peptide ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Genomics 2023Quote: ... were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter, BD Biosciences). 10 µL of RLT+ lysis buffer (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... we prepared a cranial window assembly by activating the surface of a circular glass coverslip (5 mm diameter, 0.13-0.15 mm thickness; Assistent Karl Hecht) with a laboratory corona treater (BD-20ACV Electro-Technic Products). We then fused the activated glass coverslip to a cylindrical silicone plug (1.5 mm diameter ...
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bioRxiv - Immunology 2023Quote: ... and stained for the following surface markers on ice for 30 min: CD4 (APC-Cy7, 1:100, clone RM4-5, BD Biosciences, 565650), CD8b (Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2023Quote: ... of 6 weeks old were inoculated subcutaneously (s.c.) with 5 × 105 cells mixed 1:1 with BD Matrigel Matrix High Concentration (BD Biosciences, Erembodegem, Belgium) previously diluted 1:1 in culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked with 5% skimmed milk in TBS-Tween and incubated with anti-αSyn clone 42 (BD Biosciences, 1:1,000 dilution). In a slot blot ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with PBS+5% FBS and stained with 1:100-dilutions of AF647-conjugated anti-PAX-6 (BD Biosciences; 562249) and PerCP-Cy5.5-conjugated anti-SOX-2 (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... the suspensions of Tol 5 and its mutants were placed into a 96-well polystyrene (PS) microplate (353072; Becton, Dickinson and Company) and a 96-well glass plate (FB-96 ...
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bioRxiv - Plant Biology 2020Quote: 10-fold serial dilutions (OD600=1, 10-1, 10-2, 10-3) of yeast sample harbouring a DNA-binding domain (BD)-AtCIPK16 and either an activation domain (AD)-AtCBLs or an empty vector (AD ...
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bioRxiv - Bioengineering 2022Quote: ... Brooks Lifesciences) were coated with 200 μL of 2 mg/mL type 1 rat tail collagen (354249, Corning/BD Biosciences) diluted in DMEM equilibrated in 1/10 v/v of 7.5% w/v sodium bicarbonate solution (#25080-060 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were fixed in 2% PFA and subsequently acquired using a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA). Flow data were analyzed using FlowJo v10 software (Tree Star ...
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bioRxiv - Immunology 2021Quote: ... cells were either resuspended in 400μl of PBS 2%FCS 2mM EDTA and analyzed on Fortessa-X20/Symphony cytometers (BD Biosciences) or in RPMI media and used for cell sorting on a FACSAria II (BD ...
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bioRxiv - Immunology 2021Quote: ... Vα7.2+ cells were pre-incubated for 1 h and 30 min with LukED at 0.312 μg/mL before adding to the bacteria-pulsed THP-1 and co-culture at 2:1 ratio for 24 h in the presence of 1.25 μg/mL anti-CD28 mAb (L293, BD Biosciences). Monensin and brefeldin A (both from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... whole blood was stained within 2 hours of blood draw for the surface markers CD45 (PerCP; DO58-1283; BD Biosciences), CD3 (FITC ...
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bioRxiv - Microbiology 2019Quote: ... cells were fixed in 2% paraformaldehyde and were examined in a Canto II flow cytometer (BD Bioscience, San Jose, CA), following a previously reported protocol [49] ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mL of cells at 100,000 cells/mL in PBS-BSA was loaded in a 3 mL syringe (BD, #309657). A 125 μm Drop-seq microfluidic device was used for droplet generation (31) ...