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Citations for Becton, Dickinson and Company :
2701 - 2750 of 3834 citations for Recombinant Human CD33 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Neuroscience 2023Quote: ... all samples were filtered through a 70-μm nylon mesh and blocked with anti-Fc CD16/32 (1:40; BD Biosciences). For FOXP3 staining ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 parasites were collected and incubated with VSG2WT antisera (1:4000) or VSG11WT (1:1000) together with Fc block (1:200, BD Pharmingen) in cold HMI-9 without FBS for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... CD45—CD38—CD235a—CD71— non-hematopoietic cells were sorted in DMEM supplemented with 10% FCS using a FACSAria III (BD Biosciences) and BD FACSDiva version 5.0 (BD Biosciences).
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for 15 min at 4 °C with a purified mouse anti-rat CD32 FcγII (clone D34-485, Rat BD Fc Block) to block unspecific binding ...
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bioRxiv - Cell Biology 2023Quote: Unfractionated BM cells were counted on the XP-300-Hematology Analyzer (Sysmex Corporation) and then Fc-blocked either with purified CD16/32 (BD Biosciences), or stained with CD16/32 antibody conjugated to a fluorophore ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained on ice for 30min in FACS buffer with 1:100 dilutions of biotin-conjugated-HA and purified rat anti-mouse CD16/CD32 (Mouse BD Fc Block™, Clone 2.4G2, BD Biosciences), then stained on ice for 30min in FACS buffer with 1:1000 dilution of allophycocyanin (APC)-conjugated streptavidin ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Immunology 2023Quote: ... at 1:600 antibody to cell suspension volume ratio in 30×106 cell/ml HBH with Fc block for 15-30 min on ice and then sorted with a BD FACS Aria III (BD Biosciences) with assistance from the University of Washington Pathology Flow Cytometry Core Facility ...
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bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were incubated with Fc block (anti-mouse CD16/CD32, 1 μg for 1x106 cells, BD Biosciences, Cat# 553142) at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... Cells were acquired in BD LSR-II flow cytometer using FACS diva software and FCS files were analyzed by the Flowjo v10.5.0 software (BD Biosciences, CA, USA). For absolute counting ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Developmental Biology 2019Quote: ... Fluorescein labeled DBA-lectin (1:500, FL-1031-2, Vectorlabs) and CD31 (1:300, BD 553370, BD Biosciences). For IF on paraffin sections (6 μm) ...
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bioRxiv - Developmental Biology 2019Quote: ... Fluorescein labeled DBA-lectin (1:500, FL-1031-2, Vectorlabs) and CD31 (1:300, BD 553370, BD Biosciences). For IF on paraffin sections (6 μm) ...
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bioRxiv - Cancer Biology 2020Quote: ... CFSE labeled CD8+ CTLs were subsequently sorted on a BD FACS Aria III (BD Biosciences, San Jose, CA) equipped with a 70μm nozzle.
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bioRxiv - Bioengineering 2022Quote: Cells were incubated with fluorochrome-labeled antibodies for 30 minutes at 4°C in binding buffer (BD Biosciences), prior to analysis using a BD Accuri™-B6 flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... MDA-MB-231 and MCF7 cells were stained with PE-labeled anti-MICA/B (clone 6D4, BD Pharmingen) and PE-Cy5-labeled anti-HLA-ABC (G46-2.6 ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained with fluorescently labeled antibodies (1:200) (Table 4) and sorted on FACSAria Ilu (BD Biosciences), FACSAria Fusion (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: The cells were collected and labeled using fluorochrome-conjugated monoclonal antibodies recognizing antigens (CD11b-PE, BD Biosciences #553311) at 4 °C for 15 min ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To detect cells labeled with FAP we used BV421 anti-rabbit secondary antibody (BD Biosciences #565014; 1:50). Live cells were selected based on Propidium Iodide staining (Biolegend #421301) ...
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bioRxiv - Immunology 2020Quote: ... Bespoke overlapping Treg panels were constructed using the fluorochrome-labeled antibodies listed in the Supplementary Methods (BD, Biolgend). Intracellular staining was performed using the FoxP3 staining kit (eBiosciences ...
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bioRxiv - Cell Biology 2022Quote: ... A single colony containing the fluorescently-labeled pCherry3 vector was inoculated into 10 ml 7H9/ADC (BD Biosciences) / 0.05 % Tween 80 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Neutrophils containing ingested labeled conidia were sorted using the BD FACSAria™ III (BD Biosciences, San Jose, CA). BAL Lactate dehydrogenase (LDH ...
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bioRxiv - Systems Biology 2023Quote: ... and anti-CD25+ (PE) or received a single staining with anti-CD19+ (PE) labeled antibodies (BD Biosciences, Belgium). Percentages of labeled cells were determined by an LSR Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... the plate was incubated with biotin-labeled detection antibody rat anti-mouse IgG1 (clone A85-1; BD Pharmingen) at a concentration of 1 µg/ml diluted in blocking solution for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences) for 30 minutes at 4°C.
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then evaluated stained with fluorescently labeled antibodies recognizing T cell activation markers: CD69-BV711 (BD Biosciences) and CD107a-APC (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 30 minutes with fluorochrome-labeled mAbs directed to interferon gamma (clone B27, BD), IL-17 (clone eBio64DEC17) ...
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bioRxiv - Physiology 2024Quote: ... and signals from cells labeled with conjugated fluorophores were detected by using BD FACS CantoII Analyzer (BD Biosciences). The antibodies used for different flow cytometry analysis are listed in Supplementary Table S5 ...
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bioRxiv - Immunology 2024Quote: ... The sections were stained overnight at 4°C with the following antibodies: BV421-labeled SIRP⍺ (P84, BD Biosciences), Pacific Blue–labeled B220 (RA3-6B2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Bru-labeled RNA was then captured from 100 µg of total RNA using anti-BrdU antibodies (BD Biosciences). Stranded library preparation was carried out according to documents linked to each experiment on the ENCODE portal (encodeproject.com ...
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bioRxiv - Immunology 2020Quote: ... and then labeled cells were incubated in the presence of recombinant IL-15/15Rα (50ng/mL) in the presence of absence of anti-integrin α1 (10μg/mL, BD Biosciences) or Integrin α1β1 inhibitor Obtusatin (10nM ...
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bioRxiv - Microbiology 2022Quote: ... were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective LB agar plates (BD, 244520) and in LB broth (Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... α7 integrin-PE antibody (AbLab, Vancouver, Canada), CD9-APC antibody (eBioscience, San Diego, CA) and CD47-BV605 antibody (BD Biosciences, San Jose, CA) for 30 minutes on ice and washed ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was removed and cells were resuspended in 50 µL of APC-conjugated anti-CXCR4 antibody (clone 12G5, BD Biosciences, catalogue number 560936) diluted 1:50 in FACS buffer (PBS + 0.5% BSA) ...
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bioRxiv - Cell Biology 2020Quote: ... and rat α7 integrin-APC (1/1000; AbLab, Vancouver, Canada) and sorted using a FACS Aria II (BD Biosciences, San Jose, CA, USA). Satellite cells were isolated as CD31− ...
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bioRxiv - Immunology 2022Quote: ... cells were detached using TrypLE™ Express Enzyme (1X), no phenol red, and stained with anti-CD31 (APC, Anti-Mouse) (BD Pharmingen™) MHCI (A488 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained with either BD Pharmingen™ APC Rat Anti-Mouse CD8α antibody or BD Horizon™ BV605 Rat Anti-Mouse CD4 antibody (BD Biosciences), at a final concentration of 1:1000 or 1:200 respectively ...
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bioRxiv - Immunology 2023Quote: ... cells were washed in perm buffer (PBS, 0.5% BSA, 0.1% Saponin) and stained for IFN-γ (APC rat anti- mouse IFN-γ; 1:100; BD Biosciences, Cat. 554413) and TNF (PE rat anti-mouse TNF alpha ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... GolgiStop solution was added (containing Monensin protein transport inhibitor) to block intracellular protein transport (BD Bioscience). As a positive control for cytokine production ...
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bioRxiv - Microbiology 2020Quote: Human lymphocyte characterization was performed on an LSR II (BD Biosciences). Lungs from BLT mice were minced using scissors and incubated in medium containing Liberase (0.02 mg/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was subsequently incubated with mouse anti-human CD63 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... 2.5 μL mouse anti-human CD36-PE (BD® cat. 555455), 5 μL mouse anti-human CD45-V500 (BD® cat ...
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bioRxiv - Immunology 2021Quote: ... 5 μL mouse anti-human CD45-V500 (BD® cat. 560777), and 1.25 μL rat anti-human CD294-Alexa Fluor 647 (BD® cat ...
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bioRxiv - Immunology 2021Quote: ... after which plates were incubated with mouse anti-human biotin (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... 5 μL mouse anti-human CD19-V450 (BD® cat. 560353), 2.5 μL mouse anti-human CD36-PE (BD® cat ...
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bioRxiv - Systems Biology 2020Quote: ... BV786 Mouse Anti-Human CD38 antibodies (all antibodies from BD Biosciences) for 30 min at room temperature in the dark ...