Labshake search
Citations for Becton, Dickinson and Company :
2501 - 2550 of 3834 citations for Recombinant Human CD33 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... and 3×106 cells per tube were stained as follows: cells were incubated in 0.5 μg Fc block (BD Biosciences) for 15 min at 4°C in staining buffer (Ca2+ - and Mg2+ - free PBS containing 3% heat-inactivated fetal bovine serum ...
-
bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using the anti-mouse CD16/CD32 (FcBlock clone 2.4G2) monoclonal antibodies (BD Biosciences, #553141, 1/400) and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... Single-cell suspensions were stained with 5 μM Cell-ID Cisplatin (Fluidigm Corp.) and incubated with Fc block (BD Biosciences), followed by surface antibody cocktail ...
-
bioRxiv - Neuroscience 2022Quote: ... Unspecific binding of antibodies was blocked by previous incubation for 10 min with anti CD16/CD32 (Fc block, clone 2.4G2; #55314, BD Pharmingen) in Fluorescence Activated Cell Sorting (FACS ...
-
bioRxiv - Immunology 2020Quote: ... Cells were washed and incubated (15-20 min., on ice) with Fc Block (1:100 in 1% FBS/PBS, Clone 2.4G2, BD Biosciences), followed by anti-CD45-PE-Cy5.5 (1:100 ...
-
bioRxiv - Cell Biology 2020Quote: ... 3×106 cells were incubated with purified rat IgG2b anti-mouse CD16/32 receptor antibody as per supplier’s instructions (Mouse BD Fc-block, clone 2.4G2, BD Biosciences, 553141) for 20 minutes at 4°C ...
-
Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... The liquid was poured off from the cells (leaving approximately 100 µl in each tube) and 5 µl of mouse Fc Blocking Reagent (rat anti-Mouse CD16/CD32 BD) was added to samples and incubated for 5 minutes at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
-
bioRxiv - Immunology 2021Quote: Surface staining was done in the presence of anti-CD16/32 antibodies to block FcγRII/III receptors using Mouse Fc blockTM (BD Biosciences). Cells were incubated with the Mouse Fc blockTM for 10 min followed by incubation with fluorochrome-conjugated pre-titrated antibodies for 20 min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... Pellets were suspended in 100 μl of flow buffer and blocked with 0.25 μg Fc block (BD Pharmingen, CA, USA) for 10 min followed by staining with antibodies ...
-
bioRxiv - Immunology 2020Quote: ... Samples were then washed with 2% and stained with 2% FCS in PBS and subsequently washed stained with goat anti-rabbit IgG –FITC secondary (BD) for 30 minutes at RT ...
-
bioRxiv - Neuroscience 2019Quote: Cell pellets were eluted in FACs buffer (0.5% BSA in PBS with 2mM EDTA) and blocked for ten min with Fc preblock (CD16/CD32, BD 553141), followed by 20 minute staining with anti-CD31-APC (1:100 ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were resuspended in PBS with 1% FCS and analyzed for GFP and AF647 fluorescence on a FACS Verse (BD). Nontransfected and non-AF647-stained cells were used for gating the cells positive for GFP and AF647 ...
-
bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked with 3% (w/v) bovine serum albumin (BSA, ChemCruz) in PBS with 0.5 g/mL Fc Block (BD Biosciences) for 30 minutes ...
-
bioRxiv - Immunology 2021Quote: ... All samples were then incubated with Fc block (5 μg mL-1 CD16/CD32 (BD Biosciences, San Diego, CA, USA) and 0.1 % mouse serum in FACs buffer (PBS containing 0.5 % BSA and 2 mM EDTA (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then treated with dasatinib for 1 hr at 37°C prior to blocking with Fc-block (BD Bioscience), 5 % normal mouse serum ...
-
bioRxiv - Immunology 2021Quote: ... Surface antibody cocktail was prepared in cell staining buffer (Fluidigm) and added to all samples equally after Fc block (BD). Cells were washed then fixed and permeabilized using eBioscience Transcription Factor Fix/Perm Buffer followed by barcoding (Fluidigm) ...
-
bioRxiv - Genomics 2021Quote: ... Cell counting was performed by counting of the events with FCS ≥ 1.104 and SSC ≥ 5.105 by flow cytometry (BD-Accuri-C6). The transposition activity was represented as the ratio of the hygromycine-resistant colonies to the total cells ...
-
bioRxiv - Immunology 2022Quote: Single cell suspensions were first incubated for 10 minutes on ice with anti-mouse CD16/32 Fc Block (BD Biosciences), and subsequently stained with fluorophore-conjugated antibodies against surface markers at 4°C for 20 mins ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cells were then resuspended incubated with anti-mouse CD16/CD32 at 1:400 (BD Fc BlockTM, BD Biosciences #553142) for 10 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cells were then resuspended incubated with anti-mouse CD16/CD32 at 1:400 (BD Fc BlockTM, BD Biosciences #553142) for 10 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were counted and resuspended to 10×106 cells/100μl in 1:50 Rat Anti-mouse CD16/CD32 Moue Fc Block (BD Biosciences) in FACS buffer (3% FBS ...
-
bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed using ammonium chloride and Fc receptors were blocked using anti-mouse CD16/CD32 (BD Bioscience) before staining.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL of a 1:50 dilution of Mouse BD FC block (BD Pharmigen, San Joe, USA). Cells were then stained using the following fluorescent antibodies ...
-
bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were washed with 1XPBS-4%FBS before incubation for 20 min on ice at 5•107 cells/ml with 500 ng/ml Fc block (2.4G2, BD Pharmingen). Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells (1.5 x 106 cells) first underwent Fc receptor blockade with rat anti-mouse FcψRIII/II receptor (CD16/32; BD Biosciences). After blocking for 5 minutes on ice ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
-
bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
-
bioRxiv - Immunology 2022Quote: ... Fc blocking was performed with a combination of antibodies diluted in FACS buffer with Brilliant Violet Stain Buffer (BD Biosciences) for 30 minutes at 4°C in the dark ...
-
bioRxiv - Immunology 2023Quote: ... the stained cells were stored in DPBS supplemented with 2.5% (v/v) paraformaldehyde (PFA) and 1% FCS before flow cytometry measurement on a BD LSRFortessa flow cytometer (BD Biosciences). pMHC-I multimer binding T cells were identified by a Boolean gating strategy in FlowJo (BD Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... The eluted macrophage fraction was resuspended in a final volume of 200μl MACS buffer and incubated 10 min with Fc block (1:50, BD Pharmingen). 200μl of staining solution containing flow cytometry antibodies (anti-CD45-PECy7 (1:100 ...
-
bioRxiv - Cancer Biology 2023Quote: Spleens and livers were harvested into ice cold FACS buffer (PBS supplemented with 2% FCS v/v) and then emulsified through a 70μM filter (BD Biosciences), centrifuged and washed in FACS buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in PBS + 3% FCS for cell sorting on the BD FACS Aria Fusion (BD Biosciences) at the NIHR Cambridge BRC Cell Phenotyping hub ...
-
bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
-
bioRxiv - Systems Biology 2023Quote: All flow cytometry raw data were saved as .fcs files and processed by the FlowJo software (version 10, BD Biosciences) for further analysis ...
-
bioRxiv - Immunology 2023Quote: ... Surface antigens were stained for 30 min at 4°C with indicated antibodies (Table.1) after Fc blocking for 4°C 30 min with anti-mouse CD16/32 (BD) without wash ...
-
bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
-
bioRxiv - Immunology 2023Quote: ... Single cell suspensions were incubated for 15 minutes at 4°C with anti CD16/CD32 Mouse BD Fc Block diluted 1/50 (BD) in 100 µL of FACS buffer in 96 wells round-bottom plates (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: Surface staining was performed using the following fluorescence-labeled antibodies against: CD4 (RM4-5; Biolegend, BD Biosciences), CD5 (53-7.3 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Sorting of PKH26-labeled LRC and nLRCs was performed with a FACSAria cell sorter (BD Bioscience, Germany).
-
bioRxiv - Cell Biology 2022Quote: ... Then cells were dissociated and labeled with antibodies against ganglioside GD2 (BD Pharmingen; Palo Alto, CA, USA) and low-affinity nerve growth factor receptor (NGFR ...
-
bioRxiv - Immunology 2020Quote: ... then labeled with extracellular antibodies including: CD3 (clone 145-2C11) and CD8a (clone 53-6.7) (from BD); CD11a (clone M17/4 ...
-
Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Cells were resuspended in media containing CD107a-FITC labeled antibody (Clone H4A3, BD Bioscience, San Jose, CA) and BD GolgiStopTM (protein transport inhibitor containing monensin ...
-
bioRxiv - Biochemistry 2020Quote: ... The Golgi apparatus was labeled with a rabbit anti-GM130 antibody (1:1000, BD Biosciences, Mississauga, ON). The secondary antibodies used were Alexa 488 goat anti-rabbit IgG ...
-
bioRxiv - Cell Biology 2021Quote: ... FUGW-d2GFP-ZEB1-GFP+ labeled and GFP+ pHAGE cells were isolated using FACSMelodyTM Cell Sorter (BD Biosciences). pTRIPZ-expressing cells were selected with 0.5 µg/mL puromycin.
-
bioRxiv - Biochemistry 2021Quote: ... and photoreceptor cells were labeled with PE rat anti-mouse CD73 antibody (TY/23, BD Pharmingen, USA.) for 30 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: The same bead and antibody labeled virus samples were acquired on an LSR Fortessa (BD Biosciences, USA) and CytoFLEX S (Beckman Coulter ...
-
bioRxiv - Immunology 2023Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
-
bioRxiv - Immunology 2022Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).