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Citations for Becton, Dickinson and Company :
2501 - 2550 of 3199 citations for 4 nitro 5 ethyl 2 methylpyridine n oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells or MNC from CML patients or HD were incubated with the following antibodies: 5 µL CD45-PerCP (2D1, BD Biosciences), 2.5 µL CD34-FITC (AC136 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell clusters were washed 3 times with ice-cold HBSS containing 5% FBS and filtered through 100-μm cell strainer (BD Biosciences). The cell suspension containing acini was carefully layered on top of HBSS containing 30% FBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were implanted with 5 x 104 MDA-MB-231 breast cancer cells in 50% cell culture medium (FBS free) and 50% Matrigel (BD biosciences) by subcutaneous injection in the left dorsal flank ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were incubated for 25 mins at 37°C 5% CO2 and then fixed and permeabilized with CytoFix/CytoPerm (BD Biosciences) at room temperature for 20 mins ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 Spheroids resuspended in serum-free medium were seeded in an insert that has been coated with Matrigel (BD Biosciences). Lower compartment was added with medium containing 20% FBS ...
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bioRxiv - Microbiology 2021Quote: ... 20µL were transferred into 180µL of 10mM Tris-1mM EDTA (for DiOC2(3) staining) or PBS (for DiSC3(5) staining) and analyzed by flow cytometry using LSR Fortessa (BD Bioscience) operated by FACS Diva software (BD Bioscience) ...
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bioRxiv - Microbiology 2021Quote: ... Salivary glands were cleanly dissected from infected mosquitoes placed into 5 µl of L-15 on a MaTek well (MaTek #P35G-0-14C) followed by the addition of 5 µl of matrigel (BD #356231). The matrigel was allowed to polymerize at room temperature for ∼10 minutes and Z-stacks of salivary glands were acquired using a 3i spinning disk confocal microscope (Zeiss Axio Observer Z1 microscope with Yokogawa CSU222 spinning disk) ...
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Cell Biology 2022Quote: ... filtered with a 3 μm filter and sorted for YFP expression into a 96 well plate (5 and 10 events; 48 wells each) (FACSARIA III, BD Biosciences). Individual plaques were screened by PCR and all modified loci were sequenced to confirm correct modification ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were transferred to a 50 ml tube and 9 ml LB (10 g/l Peptone (OXOID, LP0037), 10 g/l NaCl (ACROS, 207790010) and 5 g/l Yeast Extract (BD, 211929)) were added with 50 μg/l kanamycin (ACROS ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Immunology 2022Quote: ... overlapping peptides (10µg/mL) or media alone for 60 hours at 37 °C and 5% CO2 with proliferation dye (Violet Proliferation Dye, BD Biosciences). Peptides were generated in 15mer format with 8 amino acid overlaps for the ID91 fusion antigen ...
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bioRxiv - Microbiology 2022Quote: ... were grown in in Pro99 media supplemented with 5 g L-1 peptone and 1 g L-1 yeast extract (BD Difco). All heterotrophs were grown at 24 °C ...
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bioRxiv - Genomics 2022Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a LSR II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in 3ml FACS buffer before being fixed in 1% paraformaldehyde and analyzed within 5 days using a Fortessa Flow Cytometer and BD FACSDiva Software (BD Biosciences). Flow cytometric data were analyzed using FlowJo™ (Treestar ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
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bioRxiv - Cancer Biology 2020Quote: ... and the fraction of sgRNA-expressing cells was measured 3 days and 5 days after treatment as BFP-positive cells by flow cytometry on an LSR-II (BD Biosciences). Specifically ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells (5 × 104 cells per well) were plated in 24-well culture plate and the top chamber (24-well insert; BD Bioscience) were seeded with MCF7 (2 × 104 cells ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Bioengineering 2021Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Blood samples were diluted 1:5 with PBS and 10 µl aliquots were added to 1 ml of BD Retic-Count reagent (BD Biosciences). After a 60 min incubation in the dark at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences). Data was analysed using FlowJo (v 10.4).
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bioRxiv - Cell Biology 2022Quote: ... Non-adherent cells transduced with PMG based vectors were typically sorted for top 5-20% ZS-green expressing cells once on a FACSAria III (BD Biosciences).
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bioRxiv - Pharmacology and Toxicology 2019Quote: A week after tumor inoculation (5×103 344SQ-GFP/fLuc cells in 50 μL of 1:1 mix of HBSS and BD Matrigel), the animals (n=13 ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were resuspended with blocking buffer (5% BSA in DPBS) and stained with PE-Cy7 rat anti-CD31 (1:400, 561410, BD biosciences), APC mouse anti-EpCAM (1:400 ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were trypsinized and resuspended in serum-free media supplemented with 5 mM EDTA prior to measurement on an Accuri C6 plus cytometer equipped with 488 and 640 nm lasers (BD Biosciences). Untransfected cells were gated out during analysis.
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bioRxiv - Immunology 2019Quote: ... 7H9 containing 0.1% SDS in the required plate and incubating for 5 min and plating on 7H11 agar plates supplemented with OADC (BD Difco). The plates were incubated at 37°C to allow bacterial growth ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5 million 105K cells or ELT3 cells expressing luciferase31 were subcutaneously injected in a 1:1 mixture with matrigel (BD #356237) into the flank of 6-7 week old NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG ...
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bioRxiv - Microbiology 2020Quote: ... pigrum strains were cultivated from frozen stocks on BBL Columbia Colistin-Nalidixic Acid (CNA) agar with 5% sheep blood (BD Diagnostics) for 2 days ...
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bioRxiv - Immunology 2021Quote: ... lamina propria cells were stimulated for 5 h with PMA (50 ng/ml) and ionomycin (500 ng/ml) in the presence of BD Golgi plug (BD Biosciences) followed by fixation and permeabilized using the BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Immunology 2019Quote: ... for 5 min at room temperature before acquisition and sorting of the cells using a BD FACSARIA III (BD BioSciences, USA). Single antibody staining was prepared in parallel to assess fluorescence spillover ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 5 μg/mL puromycin and RFP+ cells were collected using fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were plated at a density of 1.5×106 cells/well on 18mm coverslips (Neuvitro 18-GG-PDL) precoated with high concentration growth factor-reduced Matrigel (BDBiosciences, 354263) and cultured at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked for 5 minutes at room temperature using anti-mouse CD16/32 Fc block (1/50) (BD Biosciences). Cells were then incubated with the staining (Ly6g-APCCy7 (1A8) ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 μL of the cell suspension (more than 1×105 cells) was transferred to a 5 mL FACS tube (BD Biosciences) and stained in duplicate with 10 μL of FITC Annexin V conjugate and 10 μL of propidium iodide (PI ...
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bioRxiv - Microbiology 2020Quote: ... A minimum of 5 000 – 10 000 cells was analyzed per sample for YFP expression on a LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing Software (Version 2.5).
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bioRxiv - Immunology 2022Quote: ... and tissue-resident alveolar macrophages (TRAMs) were flow-sorted using a BD FACSaria III 5-laser cell sorter and 100 μm nozzle (BD Biosciences). Of note ...
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bioRxiv - Microbiology 2021Quote: ... 0.5–1 million cells were stained with one of the following primary antibodies: mouse anti-PSGL1 antibody (KPL-1) (BD Pharmingen), mouse anti-CD43 antibody (1G10 ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Microbiology 2020Quote: ... The resulting bacterial suspension was serially diluted and plated on BD Group A Selective Strep Agar with 5% Sheep Blood (BD Diagnostics) or THY agar plates with appropriate antibiotic selection ...
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... CD8+EGFP-Tconv and CD8+EGFP+ Treg cells in blood and spleen as well as the expression levels of Stat-5 phosphorylation (clone pY694, BD Biosciences) and CD25 (clone OX39 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
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bioRxiv - Immunology 2021Quote: ... All samples were acquired with a FACS Canto II or 5 laser Fortessa with BD FACS Diva software and analysed with FlowJo software (versions 9 and 10; BD Biosciences).