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Citations for Becton, Dickinson and Company :
2551 - 2600 of 2670 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibody solutions for 5 days at 4°C: mouse monoclonal antiNMDAR1 (GluN1) (1:200; 556308, PharMingen, Becton, Dickinson and Company, Frankline Lakes, USA) or rabbit polyclonal antiNMDAa1 (GluN2A ...
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bioRxiv - Cell Biology 2024Quote: ... All tubes were centrifuged for 4 minutes at 2,000 RPM and the cells were resuspended in 200 μL of Antibody buffer (BD Perm/Wash buffer + 10 μL goat serum) with or without BRN3A antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... MM1.S cells incubated overnight in the presence of Cy5-labeled DNA ODN were fixed with 4% paraformaldehyde and then labeled with anti-LAMP1 antibody (BD, cat no. 555798, 1:100 dilution) followed by staining with 1 µg/mL DAPI.
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bioRxiv - Cancer Biology 2021Quote: ... The bottom layer of soft agar was prepared by mixing 2 × growth medium and 1.5% noble agar (BD Biosciences, San Jose, CA) at a 1:1 ratio and the mixture was poured into 6-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used for immunofluorescence of HeLa cells and/or primary neurons were: mouse anti-Ataxin-2 (BD Biosciences 611378, 1:250), mouse anti-beta-III-tubulin (Covance MMS-435P ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
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bioRxiv - Bioengineering 2020Quote: ... we also preincubated 200 µL of 2 µmol recombinant B56 protein with 1 µmol mouse anti-B56 antibody (Clone 23/B56α BD biosciences, 610615) and 1 µmol goat anti-mouse IgG Alexa Fluor 647 (Thermo Fisher A-21235 ...
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bioRxiv - Bioengineering 2021Quote: ... albicans fungal cells (CMM16) were inoculated from a streaked plate in 2 ml yeast extract peptone dextrose (YPD) [1% yeast extract (BD Biosciences, 212730), 2% peptone (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Strains were precultured during 48h in 300 μL of Synthetic Defined (SD) medium (Warringer et al., 2011) with some modifications (2% glucose, 0.14% Yeast Nitrogen Base (BD Difco™, U.S.A.), 0.5% ammonium sulfate ...
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bioRxiv - Microbiology 2021Quote: ... the ELISA was performed by coating 96 well plates with 2 µg/mL LRRC15-His (AcroBio, #LR5-H52H3) in coating buffer (BD, #51-2713KC) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Microbiology 2020Quote: Streptococcus pneumoniae D39 (serotype 2 clinical isolate) and isogenic mutant strains were cultured in Todd-Hewitt broth (Becton, Dickinson and Company; BD) supplemented with 0.2% yeast extract (BD ...
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bioRxiv - Biophysics 2020Quote: Spectrin in neurons (Figure 2d and Supplementary Figures 2 and 5) was labelled with a mouse monoclonal primary antibody anti-β-Spectrin II (Clone 42/B-Spectrin II, BD Biosciences) for 1 h at room temperature using a 1:400 dilution in 5% BSA in PBS ...
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bioRxiv - Microbiology 2020Quote: ... 50 μL of EDTA-anticoagulated whole blood were stained with the monoclonal antibodies CD3 BV605 (SP34-2, BD Biosciences, San Jose, CA), CD4 PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... The hemocytes were collected from thirty to forty shrimps at 2 hours post injection for each sorting which was performed on a FACSMelody cell sorter (BD Biosciences, USA). The fluorescence boundary was set based on detection of the shrimp hemocyte self-fluorescence without VP injection.
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bioRxiv - Immunology 2020Quote: ... IL-2 and IFN-γ concentration secreted in cell supernatant were measured using sandwich ELISA according to manufacturer’s protocol (BD Biosciences, USA). Plates were read on a Varioskan Lux multimode microplate reader (ThermoFisher ...
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bioRxiv - Pathology 2022Quote: ... the samples were washed and resuspended in PBS supplemented with 2% FBS for analysis on a five-laser flow cytometer (BD Biosciences, Fortessa). The data were analysed with FlowJo software (TreeStar).
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Pathology 2020Quote: ... Tomato-positive and -negative cells differed by ∼2 orders of magnitude in tomato fluorescence with excitation of 554nm and emission of 581nm by Influx™ cell sorter (BD Biosciences).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS+2% goat serum and cell cycle analysis was conducted using a BD FACS Calibur (BD Biosciences, UK).
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bioRxiv - Cancer Biology 2020Quote: ... two groups of NOD/SCID female mice were orthotopically injected in the mammary fat pad #4 with 2×106 cells of either ALDH1A3 overexpressing or vector control MDA-MB-231 cells (admixed with high concentration Matrigel, BD, Cat # 354262). Mice were euthanized 49 days post cancer cell implantation and the lungs were harvested ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 2 times for 5 minutes at room temperature in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... cells were fixed for 10 min at room temperature with 2% Paraformaldehyde and analysed using LSRFortessa™ X-20 Cell Analyzer (BD Biosciences), LSR II Flow Cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were incubated in 200 μl of a mixture of BD FACS™ Permeabilizing Solution 2 and BD FACS™ Lysing Solution prepared according to manufacturer’s instructions (BD Biosciences) for 8 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... around 2×105 HEK001 cells expressed GFP (GFP+ HEK001) isolated by FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA) and cultured in one well of a 24-well plate ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Systems Biology 2023Quote: ... all strains are grown in YPD (2% w/v Bacto Peptone, BD Biosciences #211677; 2% w/v D-glucose, BD Biosciences #215520; 1% w/v Yeast Extract, BD Biosciences #212750) at 30°C.
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bioRxiv - Synthetic Biology 2023Quote: ... 2% dextrose) when containing no plasmids and in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids [BD Difco], 2% dextrose) supplemented with the appropriate yeast synthetic drop-out medium supplement when plasmid maintenance was required (SDΔW-without tryptophan ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... then fixed with 2% paraformalin and kept on ice until acquisition on a BD Symphony A3 (Becton Dickinson BD; Franklin Lakes, NJ). Data was analyzed using FlowJo 10.8.1 (RRID:SCR_008520 ...
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bioRxiv - Cell Biology 2022Quote: ... Rings were fixed after 7 days with 2% PFA and stained with rat PECAM (dilution 1:100, Rat monoclonal, clone MEC 13.3; BD Biosciences, Cat# 557355). Rings were imaged in 3D using the ZEISS Lightsheet Z.1 microscope ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with 2% FACS buffer with 50nM dasatinib and fixed using fixation/permeabilization solution (Cytofix/Cytoperm™ fixation and permeabilization kit, BD Biosciences) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the internal space of the chambers in which biofilms were cultivated measured 5000μm x 500μm x 70μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417-11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1mL syringes (Brandzig ...
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bioRxiv - Microbiology 2023Quote: ... Blood was collected via cardiac puncture using a 1mL 28G1/2 insulin syringe and added to a serum collection tube (BD Microtainer 365967). Tubes were incubated for 45 minutes at room temperature to facilitate blood coagulation and then spun at 4 °C for 10 minutes (1500 x g) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells with the highest 2-5% GFP signal were selected two to three days post transfection using a flow cytometer (BD FACSAria II). Single cells were cultured in RPMI with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were trypsinized and resuspended in PBS supplemented with 1% FBS and 2 mM EDTA and immediately analyzed by flow cytometry (BD LSR Fortessa). Data analysis was performed using FlowJo v10 (FlowJo LLC).
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: Cells isolated from crawl-out epidermal cultures were washed with PBS and rested in serum-free media with 2 x 105 cells per well in 96-well assay plate (BD falcon, #353910) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with PBS (2%FBS) and resuspended in 100ul ice-cold BD Phosflow™ Perm Buffer III (BD biosciences, USA) and incubated on ice for 30 min ...