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Citations for Becton, Dickinson and Company :
2451 - 2500 of 2670 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with drugs or DMSO 5 days after infection and the fraction of TUBB-expressing cells was measured 3 days after treatment and then every 2 days as the fraction of mCherry-positive cells by flow cytometry on an LSR-II flow cytometer (BD Biosciences). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed 3 times with PBS with Ca2+ and Mg2+ containing 2% FBS and mean fluorescence intensity per cell was measured by LSRII (BD Biosciences) flow cytometer the same day of the assay.
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bioRxiv - Developmental Biology 2022Quote: ... 2% FBS in Ca2+/Mg2+-free PBS) and DAPI negative cells were selected by cell sorting using FACSAria Fusion (BD Biosciences). Cell viability was above 70% for each sample ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Tissues were incubated at 4°C overnight with the following primary antibodies: monoclonal mouse anti-carboxyl-terminal binding protein 2 (CtBP2) IgG1 at 1:200 (612044; BD Biosciences) counterstained with goat anti-Mouse IgG1 conjugated with Alexa Fluor 568 (#A-21124) ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... AAV9-mediated Snhg1 or NC were delivered by intracardiac injection into the left ventricle in 7-day-old mice at a dose of 2 × 1010 viral genome particles per animal using an insulin syringe with a 30-gauge needle (BD Biosciences). And the adult mice received the injection at a dose of 1 × 1011 viral genome particles per animal ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were processed as previously described47 and 1-2 million cells were injected into cleared mammary fat pads in 10-20 microliters of commercial Matrigel (BD Biosciences). For ER+ tumors ...
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Microbiology 2021Quote: Mouse spleens were collected in ice-cold storage solution (PBS 2% FBS) and single-cell suspensions prepared using cell strainers (BD-Falcon). Cells were washed upon red blood cells lysis (150 mM NH4Cl ...
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bioRxiv - Immunology 2021Quote: ... single MR1-5-OP-RU-tetramer+TRAV1-2+ PBMCs from healthy donors and spleen tissues were sorted using a FACSAria (BD Biosciences) into 96-well plates ...
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bioRxiv - Immunology 2020Quote: ... PBMC (2 × 106 cells/well) were stimulated with anti-CD28 (1 μg/ml) and anti-CD49d (1 μg/ml) (FastImmune, BD Biosciences) and 1e5 M ...
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bioRxiv - Immunology 2020Quote: ... then washed with 2% FCS - PBS and stained with Mouse anti-IRF-7-PE (pS477/pS479, clone K47-671; BD Biosciences) or Mouse anti-NF-κB p65-Alexa Fluor 647 (pS529 ...
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bioRxiv - Physiology 2020Quote: ... 1 ng/mL FGF2 and 10 mg/mL gentamycin) and plated on dishes previously coated with 2% Matrigel™ (BD biosciences).
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bioRxiv - Cell Biology 2021Quote: ... macrophages were incubated with 5 μM 2’, 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min, followed by acquisition using FACS Canto I (BD Sciences) within 30 minutes of staining ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected tissue was mechanically triturated by a fire-polished glass pipette and plated onto 24-well plates coated with 2% laminin (BD Biosciences) and 1% poly-D-lysine (Sigma Aldrich) ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2020Quote: ... CD95, CD278) or intracellular proteins (BCL-2, BCL-6, CD152) and cells were acquired on an LSR II analyser (BD Pharmingen), followed by flow cytometric analysis using the FlowJo Software (FlowJo LLC ...
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bioRxiv - Biophysics 2020Quote: ... and a strain with a known binding site and repressor copy number (R) - were inoculated into individual tubes with 2 mL of Lysogeny Broth (LB Miller Powder, BD Medical) with 20 µg/mL of chloramphenicol and 30 µg/mL of kanamycin ...
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bioRxiv - Immunology 2020Quote: ... the cells were diluted in 2% exo-free FBS/PBS and analyzed on a BD-LSR II flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2021Quote: ... as a negative control in the presence of αCD28/αCD49d co-Stim antibodies (1 μg ml−1) GolgiStop (containing Monensin, 2 μmol/L), GolgiPlug (containing brefeldin A, 10 μg ml−1) (BD Biosciences) and anti-CD107α BV421 antibody (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed twice with PBS containing 2% FBS and were blocked with CD16/32 Fc block (Cat. 553142, BD Bioscience) before the analysis ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The 24-color extracellular staining master mix (Extended Data Table 2) was prepared 2X in BD Horizon™ Brilliant Stain Buffer to prevent staining artifacts from BD Horizon Brilliant dye interactions and added 1:1 to cells ...
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bioRxiv - Cancer Biology 2020Quote: Cells either from culture or from transwell assay were washed in PBS and incubated in 2% FBS in PBS with anti-CCR7-FITC (1:25, #561271, BD Biosciences) and anti-ROR1-APC (1:25 ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured in the presence of 1 μg/mL brefeldin A and monensin (00-4505-51, eBioscience) for 2 hr and were then fixed and permeabilized with Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid peroxidation was detected by measuring the fluorescence intensity of BODIPY 581/591 C11 using BD FACS Canto 2 (BD Biosciences). In each case ...
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bioRxiv - Cell Biology 2022Quote: ... Cells expressing both DOR and dCas9 were isolated using fluorescence activated cell sorting (FACS) on a BD FACS Aria 2 (BD Biosciences), serially diluted ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... thermoglucosidasius DSM2542T was routinely grown on 2-SPY medium106 at 60 °C with 150 RPM shaking or on Tryptic Soy Agar plates (BD Biosciences) at 60 °C ...
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bioRxiv - Immunology 2022Quote: ... Then live Tetr-gag+Pent-gag+CD3+CD8+ cells were sorted by flow cytometry into PBS buffer 1% BSA 2 mM EDTA using a FACSAria III (BD Biosciences) equipped with 488 ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-β2 mAb IB4 (10 μg/ml) followed by Alexa Fluor 488-conjugated secondary antibody and analyzed using a FACScan (BD Biosciences) and Attune NxT flow cytometer (ThermoFisher) ...
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bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... with a purity >95% into sterile filtered PBS with 2% FBS or acquired on a FACS Canto II or LSR fortes Analyzer (BD Biosciences). Single cells were index sorted using single cell depositor ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Immunology 2024Quote: ... Stimulated and unstimulated cells were harvested and washed with 2 mL of FACS buffer and then cells were fixed with BD transcription factor fixation/perm buffer (cat #562574, BD Bioscience) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 100 µL of biotinylated anti-IgG1 (1 µg/mL in PBS with 2% FBS, clone A85-1, BD Biosciences) for 2 hours at RT ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Microbiology 2024Quote: ... pyogenes M1T1 5448 strain (56) were grown aerobically at 37°C in Todd-Hewitt Broth supplemented with 2% yeast extract (THY, BD, France). Liquid and solid cultures of L ...
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bioRxiv - Immunology 2023Quote: ... 0.25X and 4X concentration of CD4 T cell-depleting antibody for 30 minutes at 37°C before surface staining with CD3 (clone SP34-2, BD Pharmingen), CD4 (Clone L200 ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Cell Biology 2024Quote: The human iPSC line MUSli001-A was generated from skin fibroblasts from a caesarean section [34] and cultured on a polystyrene plate coated with 2 µg/mL Matrigel (Growth Factor Reduced; Corning, BD Bioscience) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strains were derived from CEN.PK113-7D and grown at 30°C in Yeast Extract Phytone Dextrose media (YEPhD, 2% Difco™ phytone peptone (Becton-Dickinson (BD), Franklin Lakes ...