Labshake search
Citations for Becton, Dickinson and Company :
2551 - 2600 of 2675 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... The 1 mM Rhobo6 solution was then injected retro-orbitally using a 0.5 mL tuberculin syringe with 27G needle (BD, 305620). Mice were allowed to recover in their cage over 30 mins then euthanized by cervical dislocation ...
-
bioRxiv - Cancer Biology 2024Quote: ... Wells were then incubated with an HRP-coupled CD63-specific antibody (1µg/ml) and developed with OptEIA TMB substrate solution (BD Biosciences), stopped with 1 M H2SO4 and read immediately on a plate reader at 450 nm.
-
bioRxiv - Immunology 2024Quote: ... cells were resuspended in 200 μL of FCM buffer including 20 μL 7-amino-actinomycin D (7-AAD) viability staining solution (BD) and incubated for 10-30 minutes before acquisition ...
-
bioRxiv - Immunology 2024Quote: ... samples were washed once with FACS buffer and fixed and permeabilized by adding 500 μL fixation/permeabilization solution (BD Biosciences). After 20 minutes of incubation ...
-
bioRxiv - Microbiology 2024Quote: ... or bile-dilutions in Cullen–Haiser Gut (CHG) media (brain heart infusion (BHI, Bacto) supplemented with 1% BBL vitamin K1-hemin solution (BD), 1% trace minerals solution (ATCC) ...
-
bioRxiv - Immunology 2024Quote: ... Samples were stained with mAbs (30 min at 4°C in the dark) and erythrocytes were lysed (when applicable) using a FACS lysing solution (BD). Isotype-matched nonreactive fluorochrome-conjugated mAbs were used as controls ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The expansion conditions of GC organoids were conducted as follows: organoids were suspended with cell recovery solution (354253, BD Falcon), incubated for 30 min on ice in a constant shaking incubator (100 rpm) ...
-
bioRxiv - Immunology 2024Quote: ... Blood isolates were then pelleted (500xg for 5 min) and subjected to two rounds of red blood cell lysis (1X Pharm-Lyse solution; BD) prior to antibody staining for flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... followed by 30 min in blocking solution (PBS supplemented with FBS 2%, saponin 0.2%, NaAz 0.1%, 1:1000 FC blocker (BD Bioscience; 553142)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sections were then incubated overnight at 4°C in 100-200 μL of blocking solution containing Rat anti-CD8α-AF647 (1:50, clone 53-6.7, 557682, BD Bioscience) and Rat anti-CD4-PE (1:100 ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were fixed and permeabilized using BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (554714, BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight in blocking solution with the following primary antibodies (1:100): rat anti-mouse SiglecF (E50-2440, 552126 BD Biosciences), Armenian hamster anti-mouse CD80 (16-10A1 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice and incubated overnight at 4°C with the fluorochrome-conjugated antibodies and unconjugated rat and mouse IgG in Perm/Wash solution (BD Biosciences), which was followed by additional 5 min incubation in Perm/Wash solution without antibodies ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μl of platelet suspension was mixed with 40 μl of staining solution (HEPES-Tyrode buffer supplemented with 2.5 mM CaCl2, 1:50 PE rat anti-mouse CD41 antibody (BD Biosciences 561850), and 3:50 FITC Annexin V (BD Biosciences 556419)) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton-X (proliferating abl pre-B cells) or 0.5% Triton-X (MCF10A cells) in PBS and fixed with BD Cytofix/Cytoperm solution (BD Biosciences, #554722) containing 4.2% formaldehyde ...
-
bioRxiv - Bioengineering 2021Quote: ... whole blood was drawn from healthy adult volunteers into acid citrate dextrose anticoagulant tubes (ACD, Solution A; BD Franklin Lakes, NJ). Consent from the volunteers was obtained prior to the blood collection ...
-
bioRxiv - Immunology 2020Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2x105 stopping gate events (live CD3+ T-cells ...
-
bioRxiv - Plant Biology 2021Quote: ... The protoplasts were resuspended in the same solution without enzyme and sorted with the cytometry (ARIA III Cell sorter, BD bioscience) with the GFP signal and single-cell pattern to isolate the high purity (~ 100% ...
-
bioRxiv - Cancer Biology 2021Quote: ... A primary antibody dilution was prepared in the blocking solution with the antibodies diluted 1:200 for HIF1α (BD Bioscience, 610958), 1:50 for phosphor-S6 (CST ...
-
bioRxiv - Bioengineering 2022Quote: The samples were diluted in proportion of 0.5% with PBS and 10 μL of the diluted solution were sampled in every measurement using a flow cytometer (LSRFortessa4, BD, USA). GFP were excited at 488 nm ...
-
bioRxiv - Microbiology 2020Quote: ... these cells were stimulated with a 10 μg/ml solution of CD28 and CD49d co-stimulatory antibodies (both from BD Biosciences) and 1ug/ml of 15-mer overlapping peptide pools spanning either the SARS-CoV-2 spike (S) ...
-
bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A and MCF-10A MYC-ER cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). Cells were lysed in Laemmli buffer (50 mM Tris-HCl pH 6.2 ...
-
bioRxiv - Cancer Biology 2021Quote: 3D-grown MCF-10A cells were washed with PBS (1X) and the Matrigel was dissolved by incubating slides at 4°C in Cell Recovery Solution (BD Biosciences). 2D-grown HEK293T ...
-
bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after treatment with Fixation/Permeabilization solution and in the presence of Perm/Wash buffer (Cytofix/ Cytoperm kit, BD Biosciences) using anti-mouse IFN-γ FITC ...
-
bioRxiv - Microbiology 2021Quote: Mouse spleens were collected in ice-cold storage solution (PBS 2% FBS) and single-cell suspensions prepared using cell strainers (BD-Falcon). Cells were washed upon red blood cells lysis (150 mM NH4Cl ...
-
bioRxiv - Bioengineering 2021Quote: ... We incubated the ECs in a 1:500 primary antibody solution containing Mouse anti-CD144 (VE-Cad, BD Pharmingen Cat. #55561) in ADB overnight at 4 oC ...
-
bioRxiv - Immunology 2020Quote: ... mouse anti human ICAM-1 (CD54) and mouse anti human VCAM-1 (CD106) antibodies and fixed with FACS lysing solution (BD Bioscience). Surface levels were determined by flow cytometry (Gallios ...
-
bioRxiv - Genetics 2021Quote: ... at least 2x106 iPSC-PPC cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit with BD GolgiStopTM (BD Biosciences) following manufacturer recommendations ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
-
bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
-
bioRxiv - Immunology 2021Quote: ... The cells were then washed with FACS buffer and fixed and permeabilized using Cytofix/Cytoperm Solution (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then fixed with 1X BD FACS lysing solution before acquiring data on a FACSSymphony A5 flow cytometer (BD Biosciences).
-
bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice in wash buffer and residual red blood cells were lysed using BD FACS Lysing Solution (BD Biosciences) for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... samples were incubated for a further 15 minutes at room temperature with 500 μl of 1X BD Lysis solution (BD Biosciences) to lyse red blood cells ...
-
bioRxiv - Immunology 2022Quote: ... and cells were fixed and permeabilized at room temperature for 15 min with Fixation/Permeabilization Solution Kit (BD Biosciences; cat. #554714). For each sample a minimum of 1.2×105 stopping gate events (live CD3+ T-cells ...
-
bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
-
bioRxiv - Plant Biology 2023Quote: ... The flowers and the fixing solution were removed and replaced with 1 µg/mL DAPI (4′,6-diamidino-2-phenylindole, BD Biosciences) diluted in pollen isolation buffer before visualizing under a fluorescence microscope using a 420 nm filter (Zeiss LSM880 Inverted Confocal Microscope) ...
-
bioRxiv - Immunology 2024Quote: ... PMA [1μg/ml] and ionomycin [0,5μg/ml] with Golgi Plug [1:1000] and Golgi Stop [1:1000] solutions (Cytofix/Cytoperm TM Plus, BD Bioscience, ref: 555028) for 4-5h at 37°C to block Golgi’s exocytosis ...
-
bioRxiv - Biophysics 2023Quote: ... we let bead-PBS solution flow through a set of strainers with mesh sizes of 70 μm (Cell strainer 70 μm Nylon, BD Falcon) and 40 μm (Cell strainer 40 μm Nylon ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then washed with staining buffer, fixed, and permeabilized (20 min, 4°C, dark) using Fixation and Permeabilization Solution (BD Biosciences). Cells were then stained with S100A9 (Clone ...
-
bioRxiv - Cancer Biology 2023Quote: ... admixed with an equal number of HDFs expressing either S301D mutant or wildtype lamin A/C were re-suspended in 70 µl of Matrigel solution (BD Bioscience) and injected intradermally into the left and right side of the mouse back ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
-
bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
-
bioRxiv - Microbiology 2023Quote: ... PBMCs were then stained with live/dead and cell surface markers in MACS solution (Miltenyi) supplemented with 2% BSA (Miltenyi) prior to permeabilization with Cytofix/Cytoperm (BD Biosciences) and staining with intracellular markers in Perm/Wash (BD Biosciences) ...
-
bioRxiv - Bioengineering 2023Quote: ... iPSC were detached and singularised using EDTA solution (0.5 M EDTA/PBS) and replated on Matrigel (1:120, BD Bioscience, #354230) at a density of 8,000-12,000 cells/cm2 ...
-
bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... and total DNA (7-AAD 1:50 solution dilution) and analyzed by flow cytometer in a BD LSRFortessa cell analyzer (BD Biosciences) using 488 nm and 640 nm lasers ...