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Citations for Becton, Dickinson and Company :
2501 - 2550 of 2675 citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cytokine production of T cells was evaluated by surface and intracellular staining using Fixation/Permeabilization Solution Kit (BD Biosciences). Background cytokine staining was subtracted ...
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bioRxiv - Microbiology 2023Quote: ... and underlain beneath a fresh BG-110 solid medium plate containing 1.5 % agar solution (Becton, Dickinson and company, USA) with a bottom dish glass ...
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bioRxiv - Biochemistry 2023Quote: ... blocked cells were pelleted and resuspended in 100 µL of primary antibody solution (1:100 mouse-α-BrdU [BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... The pellet was resuspended with 10 ul Fc block solution (1:10 Fc block) (BD Biosciences, New Jersey, USA) for 10 min ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... These solutions were filtered through a 0.22 μm sterile filter (Argos Technologies) before loading into 1 ml syringes (BD). The aqueous solutions did not mix until droplet segmentation on the microfluidic device ...
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bioRxiv - Developmental Biology 2023Quote: ... dissociated cell clusters were fixed for 20 min at 4°C in cold BD fixation/permeabilizationTM solution (BD Bioscience). Cells were washed twice in BD Perm/WashTM Buffer (BD Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequentially incubated in blocking solution (PBS, 0,5% Tween 20,1% BSA) containing anti-BrdU primary antibody (1/100, BD Biosciences) and anti-mouse secondary antibody coupled to FITC (1/300 ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Detection was accomplished by incubating with anti-ATXN2 primary antibodies (diluted 1:500 in blocking solution, BD Biosciences 611378) overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: Peripheral blood from WT and Mrp14-/- mice was harvested and erythrocytes were lysed with lysing solution (BD FACS™). Samples were stained for CD18-FITC (5μg mL-1 ...
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bioRxiv - Microbiology 2024Quote: ... PM-WCPV-S_RBD-1) was from JPT innovative peptide solution (Berlin, Germany). GolgiStop (Catalog no. 554724) was from BD bioscience (New Jersey ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining required additional steps after surface staining using the BD Cytofix/CytopermTM Fixation/Permeabilization Solution Kit (BD Biosciences) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 0.6 g of MgSO4·7H2O, 0.1 g of CaCl2 and 1.8 mL of 5 x M9 minimal salts solution [BD Biosciences]) and an aliquot was taken for cell counting by flow cytometry (see below) ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were fixed and stained for internal markers with permeabilization solution according to manufacturer’s instructions (BD Cytofix/CytopermTM). Flow cytometry was performed using an Attune NxT Flow Cytometer (ThermoFisher) ...
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bioRxiv - Genomics 2024Quote: ... the following solutions were prepared: 1) Cell labeling buffer made of BD Stain Buffer (FBS) (BD, Cat No. 554656) with 0.1 g/l sheared herring sperm DNA (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were fixed and stained for internal markers with permeabilization solution according to manufacturer’s instructions (BD Cytofix/CytopermTM). Flow cytometry was performed using an Attune NxT Flow Cytometer (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Phenol Red-free basement membrane extract (BME)/Matrigel was obtained as an 8.9-10 mg/ml solution from BD Biosciences (San Jose ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... DNA denaturation was achieved with 0.5% BSA and 2M HCl after which cells were incubated in 1:2000 solution of anti-BrdU (BD Pharmingen) for 1h at RT ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were resuspended with a 0.5% BSA/PBS solution in a FACS tube and analyzed with a FACSAria IIIu Cell Sorter (BD Biosciences), recording 20,000 events for each sample ...
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bioRxiv - Biochemistry 2020Quote: ... The resins were pre-treated with yeast extract solution (0.1 g/100 ml H2O for 1 hour; BD Biosciences, NJ) to avoid non-specific binding of EVs to resins ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were further incubated for 1 hr in solution containing goat anti-rabbit antibody conjugated with FITC (BD Biosciences, USA). All steps were carried out at room temperature ...
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bioRxiv - Genetics 2022Quote: ... the majority of each sample was then resuspended and fixed in Cytofix/Cytoperm solution (100 μL/sample, BD Bioscience, 554722) following manufacturer’s instructions and washed in Perm/Wash buffer (2 mL/sample ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed by activating cells for 4-5h at 37°C with the Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) and subsequent fixing and staining per manufacturer’s instructions using the BD Fixation/Permeabilization Solution Kit (BD Biosciences). For intranuclear transcription factor staining ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained with hypotonic fluorochromic solution (HFS) and parameters were acquired by flow cytometry (FACSCan®, BD Biosciences, USA) and analyzed in the software FlowJoX.0.7® (Tree Star ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Immunology 2020Quote: ... antibodies were used for intra-cellular staining by using the Fixation/Permeabilization Solution Kit (BD Cytofix/Cytoperm™, BD Biosciences). For in vitro intracellular cytokine staining ...
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bioRxiv - Cell Biology 2020Quote: ... IdU and CldU were then detected with the following primary antibodies in blocking solution for 1 hour at room temperature: mouse anti-BrdU (B44) (1:25) (BD) for IdU and rat anti-BrdU [Bu1/75 (ICR1)] (1:100 ...
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bioRxiv - Plant Biology 2020Quote: In vitro mycelium diameter measurements were performed by depositing a 5 μl droplet of a 1.5 × 105 spores ml−1 solution onto 1.5 % granulated agar (BD Difco™) plates supplemented with homogenates of WL- or WL+FR-treated leaves (1 gFW ml−1) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-F4/80-PE clone REA126 and human anti-CD14-FITC from Miltenyi Biotech and 7-AAD staining solution from BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... 20µL of the resulting solution was subsequently spread onto a TSB agar plate (tryptic soy broth [TSB] plus 1.5% Bacto agar; BD Biosciences) and incubated for 16 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Spleen and lung cells that were stimulated overnight with peptide pools (as described under ex-vivo stimulation and intracellular staining) were fixed and permeabilized with Cytofix/Perm solution (BD) and then stained for intracellular cytokines ...
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bioRxiv - Immunology 2022Quote: ... Cells were allowed to migrate for 1hr at 37°C and subsequently fixed with 150 µl of fixation solution (CellFIX, BD). Number of migrated neutrophils was measured at a BD FACSCanto II for 30 sec and separated from eosinophils based on their different auto-fluorescence in the V450 channel.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were fixed with Crystal violet solution (3gm in 5% Methanol: 5% Isopropanol) (BD BBL Gram Crystal Violet, Cat # 212525) and counted manually using ImageJ ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells in the whole blood samples were lysed using a commercial BD FACSTM Lysing Solution (349202, BD Biosciences). The samples were run/acquired on a FACScanto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were decorated with the following primary antibodies diluted in a 3% BSA solution for 1h30min at room temperature: mouse monoclonal anti-TIM23 (BD Transduction Laboratories ...
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bioRxiv - Systems Biology 2023Quote: ... 4×106 cells per sample were incubated in blocking solution containing 5% fetal bovine serum and 2% FcBlock (BD Biosciences) in PBS ...
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bioRxiv - Immunology 2023Quote: ... The resulting single cell suspensions were filtered through a 70µm nylon filter and treated with BD Pharm Lyse lysing solution (BD Biosciences) to eliminate erythrocytes ...
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bioRxiv - Cancer Biology 2023Quote: ... single-cell suspensions of BILs (0.5–1×106 cells) were stained with Fixable Viability Stain 780 solution (BD Biosciences, 565388) to discriminate live and dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was Fc blocked before staining using 50 µL of 2X FC Block Solution (FACS Buffer, 1:125 dilution of anti-CD16/CD32 (BD) and incubated for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Subsequently cells were fixed and permeabilized using the BD Cytofix/Cytoperm solution as recommended by the manufacturer (BD Bioscience, USA). Afterwards cells were washed and incubated with the antibody staining cocktails for 1hour at RT in the dark.
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bioRxiv - Immunology 2023Quote: ... The protein binding capacity of the well surface was saturated with 300 μL blocking solution (PBS supplemented with 2% skim milk powder (BD) and 0.1% Tween 20 ...
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bioRxiv - Biophysics 2023Quote: The FRET A/D values were mapped onto the thermodynamic model using one fluorescence coefficient (C1) to describe monomeric and low-FRET forms of BRAF in solution (B, BD), and one fluorescence coefficient (C2 ...
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bioRxiv - Cell Biology 2023Quote: Cells were analyzed for cell cycle phase using a ClickIT EdU Alexa Fluor 647 kit (Fisher) and FxCycle PI/RNase Staining Solution (Fisher) followed by flow cytometry on an LSR-II (BD) and analysis using FlowJo.
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated for 30 minutes at room temperature or overnight at 4°C with the indicated primary antibodies diluted 1:100-1:200 in blocking solution (mouse IgG1 anti-MIC60 [110329; Abcam], mouse IgG2a anti-TIMM23 [611222; BD Biosciences] ...
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bioRxiv - Microbiology 2024Quote: ... and a small portion of the frozen glycerol solution was taken from the tubes and plated on Vibrio-selective MTCBS plates (BD Difco TCBS Agar 265020 ...