Labshake search
Citations for Becton, Dickinson and Company :
2551 - 2600 of 7962 citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
-
bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
-
bioRxiv - Bioengineering 2023Quote: ... and then cells were re-suspended in 150 μL of PBS with 5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression.
-
bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were acquired on a Cytek Aurora spectral flow cytometer using Cytek SpectroFlo software (Cytek; the HD BM samples) or a LSR Fortessa X20 (special order research product with 5 lasers; BD Biosciences) and analyzed using FlowJo software (v10.8.1 ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
-
bioRxiv - Bioengineering 2023Quote: ... was grown overnight (37°C, 4% H2, 5% CO2, 91% N2, <10 ppm O2) in Chopped Meat Carbohydrate Broth (BD297307, BD, USA). Genomic DNA was extracted from B ...
-
bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
-
bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
-
bioRxiv - Neuroscience 2023Quote: ... while 100 μg was subjected to chromatin immunoprecipitation (ChIP) using 5 μg of mouse anti-ASCL1 (Mash1) antibody (BD Biosciences, 556604) or 5 μg rabbit ant-OLIG2 (Olig2 ...
-
bioRxiv - Microbiology 2023Quote: ... The resulting colonies were inoculated into 5 mL of marine broth medium (BD Difco Marine Broth 2216; Becton, Dickinson and Company), which was shaken at 300 rpm and 25°C until bacterial growth was confirmed ...
-
bioRxiv - Immunology 2023Quote: ... the cells were stained with the corresponding FACS panels (Extended Data Table 5) at 4 °C for 30 minutes and sorted using BD Aria III (BD Biosciences) or MoFlo Astrios EQ (Beckman Coulter ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
-
bioRxiv - Bioengineering 2023Quote: ... VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences) in a 37°C humidified 5% CO2 environment for 10-11 hours and diluted in 0.9% sodium chloride to the desired optical density ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a Canto II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona or on a BD Fortessa ...
-
bioRxiv - Cell Biology 2023Quote: ... Cas9-BFP+ cells were sorted for top 5% of the brightest GFP signal simultaneously on two BD FACSAria II Cell Sorters (BD Biosciences) equipped with blue 488 nm and violet 405 nm lasers ...
-
bioRxiv - Immunology 2024Quote: ... Cells were spun down at 300 g for 5 min and supernatant was collected for analysis by mouse inflammatory cytokine bead array (CBA, BD Biosciences). Cells were resuspended and stained for CD4 and analyzed for proliferation based on CFSE decrease (Fig SI 3).
-
bioRxiv - Immunology 2024Quote: ... 50 μl of PBS-BSA containing 2.5 μl of CD11c APC antibody (clone S-HCL-3) or 5 μl of CD11c BV421 antibody (clone B-ly6 RUO) (BD Biosciences) were added to each tube ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in 1X PBS with 1X protease/phosphatase inhibitor cocktail in and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). Cell sorting was performed on BD Biosciences FACS Aria Fusion ...
-
bioRxiv - Immunology 2024Quote: ... Data was acquired using a Cytek Aurora 5-laser flow cytometer or ImageStream cytometer (CytekBio) and subsequently analyzed FlowJo V10 (BD Biosciences).
-
bioRxiv - Immunology 2021Quote: ... 558121), CD11c (clone 3.9, 748288), CD3 (clone SP34-2, 557757) and ITGB7 (clone FIB504, 555945) were purchased from BD Biosciences ...
-
bioRxiv - Bioengineering 2022Quote: Cells were fixed in 2% PFA for 15 minutes and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight ...
-
bioRxiv - Immunology 2020Quote: ... cells were fixed/permeabilized using BD FACS lysing solution and permeabilizing solution 2 according to manufacturer’s instructions (BD Biosciences) followed by intracellular staining with Ki-67-PE and t-bet-BV711 (Biolegend) ...
-
bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
-
bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
-
bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CWR22Rv1 xenografts were established in the flanks of mice by injecting ~2 million cells in 50% matrigel (BD Biosciences). When tumors reached approximately 200mm3 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in blocking buffer (PBS with 2% FBS) containing an anti-BrdU monoclonal antibody (BD Biosciences) (1:100 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were sorted at a concentration of 2 million cells per mL using FACS- Aria-III (BD Biosciences). Forward and side scatter parameters were adjusted so as to eliminate cell clumps and debris ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 2% fetal-bovine serum in PBS and analyzed using the LSRFortessa flow cytometer (BD Biosciences). A total of 20,000 events were collected for each sample ...
-
bioRxiv - Immunology 2022Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes for Spike ...
-
bioRxiv - Developmental Biology 2022Quote: PI-annexin V apoptosis assay was performed at differentiation week 2 and week 4 in normal and HGPS iPSCs-keratinocytes induction according to the manufacturer’s instruction (BD). In brief ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2×105 cells were washed with PBS1X and resuspended in 100 µL of Annexin-V binding buffer (BD Biosciences). 1 µL of Annexin-V-APC (Cat# 550474) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2% FBS) and analyzed for green and red fluorescence by flow cytometry using a BD FACSymphony instrument (BD-Biosciences).
-
bioRxiv - Cancer Biology 2021Quote: ... Spheroids were harvested the next day and transferred in a collagen type I suspension (2 mg/ml) (BD/corning) (354249 ...
-
bioRxiv - Immunology 2022Quote: ... SiglecF+) as described in Figure S7: anti-CD45-Brilliant Violet 605 (2 µg/ml, clone 30-F11; BD Bioscience), anti-CD11b APC-Cy7 (1 µg/ml ...
-
bioRxiv - Genomics 2020Quote: ... Stained cells were resuspended in FACS buffer (PBSS + 2% FBS) and analyzed by FACS using BD LSRFortessa (BD Bioscience).
-
bioRxiv - Immunology 2021Quote: ... Dishes were kept undisturbed for 2 min and the clear supernatant was pipetted out slowly into cell strainer (BD). The filtrate was collected in sterile tubes and the cells were pelleted by centrifugation at 4°C for 10min at 250×g in a centrifuge (Thermo Scientific) ...
-
bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™ ...
-
bioRxiv - Immunology 2021Quote: T-cell responses were characterized by measurement of the frequency of PBMC expressing IL-2 (PerCP5.5, MQ1-17H12, BD), IL-17a (Alexa700 ...
-
bioRxiv - Immunology 2021Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes for Spike ...
-
bioRxiv - Immunology 2021Quote: ... liver and kidneys were depleted of RBC by 2 minute-incubation in Pharm LyseTM (BD Biosciences, San Jose, CA), washed ...
-
bioRxiv - Immunology 2020Quote: ... Cells were seeded (30,000-50,000/wells) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
-
bioRxiv - Immunology 2022Quote: ... the yeast were washed twice with FACS buffer (0.5% BSA and 2 mM EDTA in 1x PBS) before analysis via flow cytometry (Accuri C6 flow cytometer, BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... CD3 (SP34-2, Cat# 557757), CD4 (SK3, Cat# 612749), PD1 (EH12.2, Cat# 564104), CTLA4 (BN13, 561717) were obtained from BD Biosciences ...
-
bioRxiv - Microbiology 2022Quote: ... RT) and resuspended in 2 ml of PBS and immediately analyzed by flow cytometry (BD FACS Canto II Instrument). For each sample ...
-
bioRxiv - Immunology 2022Quote: ... along with the pre-complexed Beta variant Spike tetramer (2 μg in 100μl) coupled to PE-streptavidin (BD Biosciences). All other aspects with cell sorting ...