Labshake search
Citations for Becton, Dickinson and Company :
2501 - 2550 of 7962 citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a LSR II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona ...
-
bioRxiv - Cancer Biology 2020Quote: ... The membrane was blocked with 5% non-fat dry milk (Marvel) and subsequently incubated (O/N, 4°C) with primary antibodies HIF-1α (BD Biosciences) Lamin A (Sigma ...
-
bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
-
bioRxiv - Cancer Biology 2020Quote: ... and the fraction of sgRNA-expressing cells was measured 3 days and 5 days after treatment as BFP-positive cells by flow cytometry on an LSR-II (BD Biosciences). Specifically ...
-
bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells (5 × 104 cells per well) were plated in 24-well culture plate and the top chamber (24-well insert; BD Bioscience) were seeded with MCF7 (2 × 104 cells ...
-
bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
-
bioRxiv - Bioengineering 2021Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 2000 (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences). Data was analysed using FlowJo (v 10.4).
-
bioRxiv - Cell Biology 2022Quote: ... Non-adherent cells transduced with PMG based vectors were typically sorted for top 5-20% ZS-green expressing cells once on a FACSAria III (BD Biosciences).
-
bioRxiv - Cell Biology 2019Quote: HeLa cells were trypsinized and resuspended in serum-free media supplemented with 5 mM EDTA prior to measurement on an Accuri C6 plus cytometer equipped with 488 and 640 nm lasers (BD Biosciences). Untransfected cells were gated out during analysis.
-
bioRxiv - Immunology 2019Quote: ... 7H9 containing 0.1% SDS in the required plate and incubating for 5 min and plating on 7H11 agar plates supplemented with OADC (BD Difco). The plates were incubated at 37°C to allow bacterial growth ...
-
bioRxiv - Immunology 2021Quote: ... lamina propria cells were stimulated for 5 h with PMA (50 ng/ml) and ionomycin (500 ng/ml) in the presence of BD Golgi plug (BD Biosciences) followed by fixation and permeabilized using the BD Cytofix/Cytoperm kit ...
-
bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
-
bioRxiv - Immunology 2019Quote: ... for 5 min at room temperature before acquisition and sorting of the cells using a BD FACSARIA III (BD BioSciences, USA). Single antibody staining was prepared in parallel to assess fluorescence spillover ...
-
bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 5 μg/mL puromycin and RFP+ cells were collected using fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were plated at a density of 1.5×106 cells/well on 18mm coverslips (Neuvitro 18-GG-PDL) precoated with high concentration growth factor-reduced Matrigel (BDBiosciences, 354263) and cultured at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... A minimum of 5 000 – 10 000 cells was analyzed per sample for YFP expression on a LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing Software (Version 2.5).
-
bioRxiv - Immunology 2022Quote: ... and tissue-resident alveolar macrophages (TRAMs) were flow-sorted using a BD FACSaria III 5-laser cell sorter and 100 μm nozzle (BD Biosciences). Of note ...
-
bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting bacterial suspension was serially diluted and plated on BD Group A Selective Strep Agar with 5% Sheep Blood (BD Diagnostics) or THY agar plates with appropriate antibiotic selection ...
-
bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
-
bioRxiv - Immunology 2021Quote: ... CD8+EGFP-Tconv and CD8+EGFP+ Treg cells in blood and spleen as well as the expression levels of Stat-5 phosphorylation (clone pY694, BD Biosciences) and CD25 (clone OX39 ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
-
bioRxiv - Immunology 2021Quote: ... All samples were acquired with a FACS Canto II or 5 laser Fortessa with BD FACS Diva software and analysed with FlowJo software (versions 9 and 10; BD Biosciences).
-
bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
-
bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
-
bioRxiv - Genomics 2022Quote: ... and then dounce homogenized (10 loose strokes and 5 tight strokes) and filtered through 35-40μm cell strainers (BD Biosciences, 352235). Nuclei were pelleted (600g ...
-
bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
-
bioRxiv - Bioengineering 2022Quote: ... The specificity of CD8+ T cells towards the peptide was previously assessed by co-culture of 5·104 T cells with pulsed tumor cell lines in the presence of Golgi Plug (BD Biosciences) in 96-well plate at 1:1 E:T cell ratio.
-
bioRxiv - Cell Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The activated Ramos cells were incubated overnight (18 h) in 5% CO2 at 37°C followed by staining for flow cytometry (BD LSR2) and analysis (FlowJo and Graphpad Prism).
-
bioRxiv - Microbiology 2024Quote: ... The antibodies employed for ELISA were biotin-conjugated rat anti-mouse IgG2a (clone R19-5, BD Biosciences, Denmark cat no. 550332) and anti-mouse IgG conjugated to horseradish peroxidase (HRP ...
-
bioRxiv - Neuroscience 2023Quote: ... cell suspension was incubated with Fc block antibodies for 5 minutes at 4 °C and then labeled with anti-mouse Thy1.2 magnetic particles (BD IMag™) for 30 minutes at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA in Dulbecco’s PBS) analyzed using the Guava® easyCyte 5 HPL benchtop flow cytometer (MerckMillipore) or BD LSRFortessaTM cell analyzer (BD Biosciences). Data analysis was performed using FlowJo version 10 (FlowJo ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
-
bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were stained with Alexa Fluor 647-conjugated rat anti-CD49f antibody (5% v/v in a 100 µL reaction; BD Biosciences) for 25 minutes at 37°C on an orbital shaker at 200 rcf and resuspended in DPBS containing 0.5% bovine serum albumin and 50 units/mL DNase I (Sigma Aldrich) ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-CD11b-FITC (clone M1/70, catalog no. 553310; BD Biosciences), anti-Ly6G-APC (clone 1A8 ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies (0.5 μg/mL) and dyes (concentrations provided below) were added: Anti-MPO-FITC (clone M1/70, catalog no. 553310; BD Biosciences), Fc Block ...
-
bioRxiv - Cancer Biology 2023Quote: ... or ASC52telo cells (ATCC SCRC-4000) were suspended in 5%FBS/PBS solution and the following antibodies were added: APC-CD14 (BD 561708), APC-CD19 (BD 561742) ...
-
bioRxiv - Cancer Biology 2024Quote: ... at 9:00 am 5 mL of fasted blood from the antecubital vein was collected into an EDTA Vacutainer tube (BD, 366643), then centrifuged at 800 x g for 10 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: Cells from BALF were centrifuged at 300 x g for 5 minutes and incubated in 500μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 minutes at room temperature to lyse red blood cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
-
bioRxiv - Neuroscience 2023Quote: ... and non-neuronal (NeuN-) nuclei were sorted into tubes pre-coated with 5%BSA using a BD-FACSAria flow cytometer (BD Biosciences) equipped with a 70μm nozzle (Figure S6) ...
-
bioRxiv - Immunology 2023Quote: ... P14 T cells (Figure 5) were FACS-sorted from the spleen or LNs of chronically infected mice using a FACSAria III cell sorter (BD Biosciences). To multiplex different samples for scRNA sequencing ...
-
bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
-
bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...