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Citations for Becton, Dickinson and Company :
201 - 250 of 269 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Immunology 2021Quote: ... lamina propria cells were stimulated for 5 h with PMA (50 ng/ml) and ionomycin (500 ng/ml) in the presence of BD Golgi plug (BD Biosciences) followed by fixation and permeabilized using the BD Cytofix/Cytoperm kit ...
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bioRxiv - Microbiology 2020Quote: ... Footpads were digested with 4 mg ml−1 collagenase D for 2 h at 37°C before dissociation through 70 μm cell strainers (BD Biosciences). Homogenates were resuspended in HOMEM supplemented with 20% FCS and serial dilutions (2-fold ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated ex vivo with PMA (50 ng/ml) and ionomycin (750 ng/ml) for 4 h in the presence of GolgiPlug (BD Bioscience), and then fixed and permeabilised following intracellular staining with anti-mouse IL-17A (clone ReBio17B7 ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and analyzed for fluorescence expression using a flow cytometer (BD FAC AriaIII sorter; BD biosciences, New Jersey, USA). A viral titration curve was constructed using viral dilutions on the X-axis and percentage EGFP+ve cells on the Y-axis ...
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bioRxiv - Neuroscience 2022Quote: ... that was blocked in 5% non-fat milk for 1 h at room temperature and incubated overnight with primary antibodies against the following proteins: α-synuclein (1:1000, BD), NCK2 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Novus) and F(ab’)2-donkey anti-Rabbit IgG (H+L) secondary antibody PE (1:100, 12-4739-81, BD bioscience) at 4℃ for 1h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The activated Ramos cells were incubated overnight (18 h) in 5% CO2 at 37°C followed by staining for flow cytometry (BD LSR2) and analysis (FlowJo and Graphpad Prism).
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bioRxiv - Cell Biology 2024Quote: Cells were seeded 24 h before treatment and were allowed to grow in the presence of 3 μg/mL BrdU (BD Pharmingen) for 48 h ...
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bioRxiv - Bioengineering 2024Quote: ... and the hemoglobin contents were quantified after 8 h by the colorimetric assay kit (absorbance: 550 nm; with BD Pharm Lyse). Each value was subtracted from the internal control without the anti-BSA antibody.
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bioRxiv - Neuroscience 2023Quote: ... single viable cells were gated and CD45-O1+ and CD45lowSiglec- H+ cells were collected using a FACSAria III and corresponding software (FACSDiva, v.6; BD Biosciences). Calculation of the number of CD45lowSiglecH+ microglia per brain was performed by extrapolating their frequency to the counted total number of isolated cells ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated with 1 µg/ml SIINFEKL peptides in complete RPMI medium for 1 h at 37°C followed by brefeldin A (Golgiplug; BD Pharmingen) addition and further 5 h incubation ...
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bioRxiv - Cell Biology 2023Quote: ... 10-μM bromodeoxyuridine (BrdU) solution was added 4 h before the end of the experiment [fluorescein isothiocyanate (FITC) BrdU Flow Kit (BD #559619)] according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were cultured for 24 h prior to FACS sorting of transduced (GFP+/YFP+) cells on a BD FACSAriaTM III (BD Bioscience). The efficiency of shRNA-mediated gene expression knockdown was assessed with qPCR and cells were used for transplantation and assessment of their competitiveness in vivo.
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bioRxiv - Molecular Biology 2023Quote: ... while the OR10a/49b/71a cell lines were sorted 48 h after doxycycline addition for GFP and mCherry fluorescence with a FACSAria III cell sorter (BD Biosciences) into 96-well cell culture microplates ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stained for 30 min at 4 °C and washed with cell staining buffer and acquired within 1 h on a custom SORP five laser FACSAria Fusion (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... prior to stimulation for 5 h with anti-CD3 and anti-CD28 without IL-2 in the presence of brefeldin A and monensin (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... Cells expressing GFP or Halo (labeled by JF549-HaloTag ligand) were subjected to single-cell sorting into 96-well plates 24-36 h after transfection (FACSAria II, BD Biosciences). Monoclonal cell populations with mutations in both alleles were identified through sequencing ...
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bioRxiv - Microbiology 2024Quote: ... in BHI medium and grown at 37°C either with aeration for 8 h or overnight anaerobically (GasPak™ EZ Systems, BD). The cells were then removed from the supernatant by centrifugation (9,000 x g ...
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bioRxiv - Microbiology 2024Quote: ... The plates were incubated for 2 h at 37 °C before the bacteria were resuspended in a final volume of 100 µl MH broth (BD Biosciences). Serial dilutions in MH broth were carried out and 5 µl of each dilution was spotted in technical duplicates on MH agar plates supplemented with 50 µg/ml ampicillin for calculation of total number of colony forming units (CFUs ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated for 4 h and washed twice with PBS for analysis of fluorescein positive cells via flow cytometer (BD FACSCantoTM II, BD Biosciences). To further investigate the internalization mechanism of PoLixNano ...
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bioRxiv - Cell Biology 2024Quote: ... was added for 1 h at 37 °C followed by Streptavidin-conjugated Horse Raddish Peroxydase (HRP; BD Pharmingen; dilution 1:3000) for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Stimulation was performed for 20 h at 37°C in the presence of 1 μL/mL GolgiPlug™ (BD Biosciences, 555029). For the unstimulated condition ...
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bioRxiv - Cancer Biology 2024Quote: ... 4:1 CTL:cancer cell admixtures were incubated for 4 h at 37°C in the presence of BD GolgiPlug protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were labeled with murine antibodies against CD3 (BV650) ...
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bioRxiv - Microbiology 2020Quote: ... The sorted GFP− cells were stimulated with 10 ng/mL TNF-α for 6 h and then GFP expression levels were analyzed using BD FACSVerse (BD Biosciences). The level of gag expression after 18-h stimulation with 10 ng/mL TNF-α was analyzed by reverse-transcription (RT)-digital droplet PCR (ddPCR) ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were ex vivo stimulated for 20 h with 40 μg/ml PPD (purified protein derivative, Statens Serum Institute, Denmark) in the presence of Golgi Stop™ (BD Biosciences) and Monensin (1000x ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... HAECs were allowed to recover from passaging for 24 h after plating and were subsequently incubated with AlexaFluor-647 anti-human VE-Cadherin antibody (1:100, BD Biosciences, #561567). After 15 minutes incubation ...
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bioRxiv - Pathology 2020Quote: ... Cells were harvested 24 h post-transfection and further subjected to luciferase and β-galactosidase activitydetection by using the D-Luciferin (BD Biosciences) and o-nitrophenyl-β-d-galactoside (ONPG) ...
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bioRxiv - Immunology 2021Quote: ... The plate was incubated 6 h at 37°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 4 h of incubation ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2022Quote: Secretion of CXCL10 (IP-10) into the medium was quantified 24 h after stimulation by ELISA (Human IP-10; BD OptEIA, 550926). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... GAS 2006 was cultured under static conditions at 37°C for 17 h in THY medium (Todd-Hewitt broth (Bacto BD, Cat# 249240) containing 1.5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% milk in 0.2% Tween-20 in TBS for 1 h at room temperature and probed with primary antibodies against SORLA (1:4000; 611680, BD Transduction Labs) and GAPDH (1:10,000 ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were gated on FSC and SSC followed by single cell gate on FSC-HH/FSC and SSC-H/SSC and a LIVE/DEAD near IR marker (1:1000, BD Biosciences, L34976). The following antibodies were used ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then incubated for 1 h with primary antibodies directed against the cis-Golgi marker GM130 (mouse anti-GM130, BD bioscience, 610822) and/or trans-Golgi marker TGN46 (sheep anti-TGN46 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked with Superblock blocking buffer in PBS/ 0.1% Triton-X for 1 h and then incubated with the following primary antibodies overnight at 4 °C: α-CD11b (1:100; BD Biosciences) and α-Iba1 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... The plate was incubated 6 h at 371°C in the presence of BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences) for the last 41h of incubation ...
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bioRxiv - Microbiology 2024Quote: Cytokine secretion by WT RAW 264.7 macrophages stimulated for 4 h with LPS was measured by ELISA using BD OptEIA™ Mouse IL-6 ELISA Kit (BD Biosciences), BD OptEIA™ Mouse TNF ELISA Kit (BD Biosciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... permeabilized with 0.1% TritonX in PBS containing 2% BSA for 15 h at 4 °C and incubated with 1:500 anti-p62 (BD Bioscience, 610833) and 1:500 periplasmic extract in blocking buffer for 2 h at 25 °C ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Cancer Biology 2020Quote: GFP-positive cells containing the PX458 vector were sorted into a 24-well plate 72 h after transfection by using a FACS Aria II cell sorter (BD Biosciences, Heidelberg, Germany). Cells transfected with the PX459 vector were selected with 1 ug/mL puromycin (Santa Cruz Biotechnology ...