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Citations for Becton, Dickinson and Company :
151 - 200 of 269 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for 4 h at 37°C in the presence of both Brefeldin A (GolgiPlug) and Monensin (GolgiStop, both BD Biosciences). As GolgiPlug and GolgiStop were used together ...
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bioRxiv - Immunology 2020Quote: ... CLTX-CAR T cells were incubated with GBM cells (E:T=1:1) for 5 h in the presence of CD107a antibody and GolgistopTM protein transport inhibitor (BD Biosciences). To test for CAR T cell killing potency ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Tconv cells were stimulated for 12 h with plate-bound 0.25 μg/mL anti-human CD3 mAb (clone HIT3α, BD Biosciences) and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2 ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Tconv cells were stimulated for 24 h with plate-bound 0.25 μg/mL anti-human CD3 mAb (clone HIT3α, BD Biosciences) and either plate-bound 3 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2 ...
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bioRxiv - Cell Biology 2020Quote: ... T cell activation was monitored 18 h later as CD69 expression by flow cytometry (FACSCalibur, BD Biosciences, San Diego, CA) and IL-2 secretion in coculture supernatants by ELISA (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Then cells were fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). For cell sorting ...
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bioRxiv - Immunology 2020Quote: ... 1–2 × 106 cells were incubated with 1 μM of SARS-CoV-2 minimal epitope or pool of up to ten epitopes (1 μM/peptide) for 9 h at 37°C in the presence of protein transport inhibitor (GolgiPlug; BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... containing fluorochrome-conjugated murine H and L isotype-specific antibodies (antimouse IgG1-APC # 550874 and anti-mouse IgG3-bio # 020620 from BD, Franklin Lakes ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was replaced with medium 12 h post-transduction and fluorescent cells were purified 24 h later by fluorescence-activated cell sorting (FACS; >95% purity on re-analysis) using a FACS Influx (Becton Dickinson, BD). K562 cells (~5×105/mL ...
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bioRxiv - Immunology 2020Quote: ... Samples were fixed with 1% freshly prepared paraformaldehyde for at least 1 h and then analyzed in a FACSCanto II flow cytometer (BD). Data were analyzed using FACSDiVa (BD ...
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bioRxiv - Immunology 2021Quote: ... bacteria were washed and fixed in 0.15 ml/sample 1% paraformaldehyde in RPMI-H and analyzed using flow cytometry (BD FACSVerse). Data were analyzed by FlowJo software and presented as Fluorescence Intensity (FI ...
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bioRxiv - Immunology 2021Quote: For intracellular staining of IgG H/L PerC and splenocytes were surface stained with anti-CD43-BV510 (S7, BD Bioscience), anti-CD19-PE (6D5 ...
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bioRxiv - Immunology 2021Quote: ... After 12 h, 1x PMA/ionomycin (eBioscience, 00-4970-93) was added to positive control wells and GolgiPlug (BD, 555029) was added at 1:1000 to all wells ...
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bioRxiv - Microbiology 2022Quote: ... The FITC-H GFP fluorescence intensity (median of the population) was measured using a FACSCantoTM II flow cytometer (BD Biosciences). The fluorescence background of a WT unlabelled strain was subtracted from the fluorescence intensity of each dctA::sfgfp carrying strain ...
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bioRxiv - Neuroscience 2023Quote: Around 15,000 CD45lowSiglec-H+ and CD45-O1+ single cells each were sorted per sample using a FACSAria III (BD Biosciences) before being encapsulated into droplets with the Chromium Controller (10x Genomics ...
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bioRxiv - Bioengineering 2023Quote: ... Mice sera were collected retro-orbitally at 4 h and 7 days post administration and subjected to cytokine analysis (Cytometric Bead Array Mouse Inflammation Kit, BD) using flow cytometry following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... T cells or NK cells were mixed with tumor cells for 6 h in the presence of GolgiPlug (BD, BDB555029). As a positive control ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with Blocking Buffer (Odyssey) for 1 h at room temperature before incubation with mouse anti-AP2A1 (BD, clone 8 ...
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bioRxiv - Plant Biology 2023Quote: ... The suspension was then incubated in propidium iodide and RNase for 1 h and detected with flow cytometry (BD FACScalibur). Tomato and barley were used as internal references.
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in 3% BSA/PBST (0.1% Triton X-100 in PBS) and incubated with the samples at 37°C for 1 h: mouse anti-BrdU (BD Biosciences 347580 ...
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bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... The presence of fibroblasts in tumoroids was determined after 24 and 48 h by flow cytometry (LSRFortessa analyzer, BD Biosciences). To measure absolute cell numbers ...
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bioRxiv - Physiology 2024Quote: ... The sections were incubated for 1 h at room temperature in 0.3% BSA in PBS with a rat anti-mouse Ly6G (BD Bioscience), a rat anti-MOMA-2 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: Venous blood was sampled from an antecubital vein between 08:00–10:00 h and collected in EDTA tubes (BD 186 Vacutainer Systems ...
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bioRxiv - Microbiology 2022Quote: ... Plates were photographed after 24 h incubation at 37°C in 5% CO2 or under anaerobiosis (GasPak jar, BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... were incubated for 1 h at room temperature followed with another incubation of diluted HRP-conjugated streptavidin (554066, BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... 10 nM ImP or 100 nM ImP for 24 h before being washed and collected into FACS tubes (BD Biosciences). Cells were incubated with following antibodies ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with individual CHIKV peptide pools (2μg/ml) for 6 h in the presence of BD GolgiPlug (BD Bioscience). Cells were harvested and stained with antibodies for CD3 ...
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bioRxiv - Neuroscience 2024Quote: For evaluation of transcellular permeability in HUVECs grown for 24 h on 0.1% gelatin-coated glass chamber slides (BD Falcon) in FA-supplemented or control media ...
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bioRxiv - Neuroscience 2024Quote: To visualize mitochondria in HUVECs grown for 24 h on 0.1% gelatin-coated glass chamber slides (4- or 8-well; BD Falcon) in FA-supplemented or control media ...
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bioRxiv - Developmental Biology 2024Quote: ... using FACS gates (FSC-A vs FSC-H; DAPI-A) to exclude debris and clumped cells (BD FACS Aria IIIu).
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Intrinsically Disordered Regions Define Unique Protein Interaction Networks in CHD Family RemodelersbioRxiv - Biochemistry 2024Quote: ... Cells were then treated with tamoxifen (100 nM) for 28 h followed by labelling with Annexin-APC (BD Biosciences, #550475) and DAPI as per manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Genetics 2022Quote: ... Cells were incubated at 37°C with 5% CO2 for 24 h and then sorted for GFP+ expression using fluorescent activated cell sorting (FACS) on a FACSAria II (BD Bioscience). The GFP+ pool (bulk ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated for 4 h with eBioscience™ Cell Stimulation Cocktail and a protein transport inhibitor-containing Brefeldin (1.5uL/mL StopGolgi; BD Biosciences). Cells were then washed in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... the killing capacity of NK cells was tested when they coculture with senescent / young or modulated for ST8SIA1 expression MRC5.NK cells are added to the culture for 4 h in presence of monensin and brefeldin (BD Biosciences) at the effector/target ratio of 1:1 ...
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bioRxiv - Bioengineering 2021Quote: ... H-2Kb-negative GFP-positive cells were sorted into single cells on a 96-well plate using FACSAria Fusion (BD Biosciences) and cultured for 2-3 weeks in D10 ...
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected at 72 h post FeNPs administration and detected with the Annexin V-FITC Apoptosis Detection Kit (BD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of GFP-positive cells among the mCherry-positive cells was determined 48 h after I-SceI transfection using a LSRFortessa X-20 (BD Bioscience) using BD FACSDiva Software v8.0.1 ...
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bioRxiv - Microbiology 2020Quote: ... lymphocytes were cultured in 96-well dishes at 37°C for 5-6 h in the presence of 2 μM peptide pool and brefeldin A (BD Biosciences). Cells were then labeled for cell-surface markers ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Bioengineering 2022Quote: ... Genes encoding the proteins were cloned into pET29b between the restriction sites NdeI and XhoI and expressed for 4 h in Terrific Broth (BD Difco) using Lemo21 (NEB ...
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bioRxiv - Microbiology 2022Quote: ... aureus was also grown in cell free supernatants (CFS) of Fusobacterium nucleatum ATCC 25586 that had been cultured anaerobically for 48 h in BBL Brucella Broth (BD 2011088) supplemented with 250 μg/mL and 50 μg/mL of hemin and vitamin K (Hardy Diagnostics Z237) ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were allowed to activate for 24 and 48 h and then stained with Annexin V FITC and 7AAD (BD Pharmingen). Cell proliferation was analyzed 96 hours post polyclonal activation in CD4+ T cells previously stained with cell trace violet dye (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... the cells were treated with 1 µg/ml doxycycline for 24 h and single GFP+ cells were sorted into 96-well plates on a BD FACSAria UV instrument (BD Biosciences) and grown until colonies formed.
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained with fluorochrome-conjugated antibodies or influenza HA-specific (HA518–526) H-2Kd tetramer-IYSTVASSL (MBL) reagent and analyzed using a flow cytometer (BD Biosciences). BMDCs (105 cells ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed once with FACS buffer and fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). In some experiments ...