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Citations for Becton, Dickinson and Company :
2401 - 2450 of 2558 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... They were finally washed with 4 mL FACS buffer and re-suspended in 0.5 mL to be analyzed in a LSR Fortessa X20 (BD Biosciences, Europe). For live staining ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: Stained cells were stored for up to 2 days at 4°C in the dark (TRAIL-R1) and flow cytometry was performed on a BD FACSCanto II (BD Biosciences). Raw data were exported to FlowJo v10.8.1 for analysis and plotting.
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence intensity of Mag-fluo-4-loaded cells was measured using a BD Accuri C6 flow cytometer (BD Biosciences, CA, USA) with an FL1 detector for green fluorescence and logarithmic amplification ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with a mouse pan α-synuclein primary antibody (Syn1, BD Biosciences, NJ, USA; #610786) diluted 1:5,000 in blocking solution ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Genomics 2024Quote: ... The cells were then washed twice with FACS buffer and then analyzed for DAPI intentsity in LSR-Fortessa 4-9 HTS bench top flow cytometer (BD Biosciences). DAPI intensity for at least 10,000 individual cells were recorded for each sample.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and fixed/permeabilized for 20 min at 4°C using the Cytofix/Cytoperm Fixation/Permeabilization Kit (BD Biosciences). Cells were washed with 1X PermBuffer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then harvested and fixed for 24 hours in 1ml fixative buffer (BD Cytofix/Cytoperm prepared 1:4 buffered to PBS; BD Biosciences) at 4°C on a slow shaker ...
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bioRxiv - Immunology 2024Quote: ... surface stained cells were fixed and permeabilized (20 min at 4°C) using Fixation/Permeabilization Solution before washes in Perm/Wash buffer (both BD Biosciences). Cells were then stained with intracellular Abs as above except in Perm/Wash buffer ...
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bioRxiv - Immunology 2024Quote: ... cell suspensions were incubated at 4°C or 37°C for 90 minutes and labelled with Annexin V-FITC or Annexin V-BV711 (BD Biosciences) in line with the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... #DY413 and #DY404) for IL-4 and IL-13 and BD™ Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD, #552364) for IL-2 ...
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bioRxiv - Bioengineering 2024Quote: ... washed and stored at 4 °C for analysis the next day on a on a flow cytometry instrument (LSR Fortessa, BD Biosciences). Flow cytometry buffer was used to prepare Fc block and antibody solutions ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue and cell slides were then blocked with 1% BSA and 10% normal goat serum in PBS at room temperature for 1 hour followed by overnight incubation at 4°C with primary antibodies (1:50 dilution) against human HIF1α (cat # 610958, BD Biosciences), rat HIF1α (cat # NB100-105 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with Fixable Viability Dye (20 min, 4°C, Fixable LiveDead, eBioscience) and then with a mix containing a brilliant stain buffer (BD Biosciences) and the surface markers for CD4+ T cells detection (CD3 and CD4 ...
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bioRxiv - Immunology 2024Quote: ... The samples were then stained with the fluorophore-conjugated antibodies or isotype controls in a flow cytometry buffer for 30 minutes at 4 °C in the dark and analyzed with the FACS symphony A1 (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... A human EC model of obesity and diabetes was established by growing HUVECs to confluency on gelatin-coated 24-well TC plates or glass chamber slides (4-well or 8-well; BD Falcon) in EGM-2 and thereafter changing to growth factor-depleted (GFD ...
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bioRxiv - Physiology 2024Quote: ... antibodies (or isotypic controls) for 30 min at 4°C before analysis was run using a FACSCanto II flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... plates were washed 4 times with PBST and filled each well with 100 µl 1000 times diluted HRP-conjugated streptavidin (BD, 554066) in 0.5% skim milk PBST buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated for 4 h and washed twice with PBS for analysis of fluorescein positive cells via flow cytometer (BD FACSCantoTM II, BD Biosciences). To further investigate the internalization mechanism of PoLixNano ...
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bioRxiv - Cell Biology 2024Quote: ... iECs were dissociated with TrypLE Express and fixed with 4% paraformaldehyde with 0.2% Triton X-100 and then the cells were filtered with 70 μm cell strainers (BD Falcon, 38030) in 1% BSA-PBS ...
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bioRxiv - Bioengineering 2024Quote: ... the frozen aliquot was thawed in a 37°C water bath for 4 min before being diluted in 35 mL stain buffer (BD, 554656) and pelleted at 300g for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody staining was performed at 4 °C for 30 min and the stained cells were analyzed on an LSRFortessa cell analyzer (BD Biosciences). FACS sorting routinely yielded cell purity levels of over 90 % ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour at room temperature and incubated overnight at 4°C with primary antibodies: N-cadherin (1:400, 610921, BD Bioscience), α5 integrin (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4:1 CTL:cancer cell admixtures were incubated for 4 h at 37°C in the presence of BD GolgiPlug protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were stained with the following antibody mixes for 1 hour at 4°C in the dark: Mix 1: AlexaFluor 700 anti-CD45 (1:150, BD Biosciences), AlexaFluor 647 anti-CD44 (1:100 ...
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bioRxiv - Physiology 2021Quote: ... Sections were then washed with PBS 3 times for 15 min and incubated with PBS containing Hoechst (1:5000, #561908, BD Pharmingen, Lincoln Park, NJ) for 5 min before a final series of washes with PBS.
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bioRxiv - Bioengineering 2023Quote: ... so that the microfluidic tubing (Scientific Commodities,Inc., BB31695-PE/3) can connect the device to the sample sources in a syringe (BD 1 mL, NJ, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The phosphorylation-SMAD2/3 ability of CAR T cells was detected using PE Mouse anti-Smad2/Smad3 antibodies (clone O72-670, BD Biosciences, Franklin Lakes, NJ) following the staining protocols ...
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bioRxiv - Bioengineering 2023Quote: ... pneumoniae serotype 3 (Sp3) (ATCC; Manassas, VA) was cultured on plates of BBL Trypticase Soy Agar with 5% sheep blood (BD Trypticase Soy Agar II) (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and F15 NOD Nfκb1 heterozygote were suspended in biotin-free RPMI containing 0.1% azide and 3% FCS and surface stained in 96-well plates with the 10–3.6 PE (anti-I-Ag7) (BD PharMingen, Franklin Lakes, NJ, USA), which is class II major histocompatibility complex (MHC ...
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Angiogenesis in the mature mouse cortex is governed in a region specific and Notch1 dependent mannerbioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight in 0.1M PBS + 0.1% TX-100 solution containing primary antibody for rat anti-CD31 (label endothelial cells; 1:200 dilution, BD Clone MEC 13:3: #553370) or sheep anti-CD93 (label endothelial tip cells ...
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bioRxiv - Pathology 2024Quote: ... phosphorylated TDP-43 (clone 11-9, 1:2000, CosmoBio, Tokyo, Japan) and anti-p62 (3/P62 LCK LIGAND, 1:500 BD Company, NJ, United States) were employed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... analysis of sub-G0/G1 DNA content as previously described (23) and by measurement of active caspase-3 by flow cytometry using a commercial kit (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Immunology 2021Quote: ... Slides were treated for 10 minutes in H2O2 and after blocking in 10% goat serum in 0.1% Tween20 for 30 minutes and incubated overnight at 4°C with specific antibody diluted in PBS 0,1%Tween20: anti-mouse Ly6G (1A8, BD Pharmingen, cat 551459), or anti-mouse Ki67 (D3B5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and incubated overnight at 4° C with primary antibodies against Syn1 (mouse, 1:1000, BD Biosciences, #610786), pSer129 (rabbit ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Bioengineering 2020Quote: ... was applied for 30 minutes at 4°C and stained cells were resuspended in FACS buffer before flow analysis on a BD FACSCanto™ II (BD Biosciences). Gating and flow data were processed on FlowJo (FlowJo LLC).
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bioRxiv - Microbiology 2021Quote: ... and several ten-fold dilutions (10−4 to 10−7) were plated onto de Man Rogosa and Sharpe (MRS) media (BD Difco, USA) supplemented with 50 mg/L of mupirocin (PanReac AppliChem ...
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Immunology 2021Quote: ... Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA).
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...