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Citations for Becton, Dickinson and Company :
2151 - 2200 of 2558 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Single GFP positive cells were sorted at 4°C using a BD FACSAria III (BD Biosciences, Franklin Lakes, NJ) and deposited in 1% gelatin-coated 96 well plates ...
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bioRxiv - Bioengineering 2023Quote: ... fixed with 4% PFA for 20 minutes at RT and permeabilized using Cytofix/Cytoperm (BD Biosciences, catalog no. 554714) for 20 min on ice followed by twice washes with 1x Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and cardiac perfusion was performed with 100 ml of 4 C PBS (0.1 M, pH 7.2) placing a 25G 0.50 mm X 16 mm syringe (BD Vacutainer, 367294) through the left ventricle of the heart to remove blood from the brain and organs ...
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bioRxiv - Immunology 2023Quote: ... Cells were labeled for 20 min at 4 °C with the following specific antibodies: BUV496 anti-CD19 (SJ25C1;BD), BUV563 anti-CD56 (MY31;BD) ...
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bioRxiv - Immunology 2023Quote: ... and IELs were stained in 2.4G2 supernatant for 30 minutes at 4°C with the following antibodies: (BD Biosciences) CD4 (RM4-4) ...
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bioRxiv - Developmental Biology 2023Quote: ... dissociated cell clusters were fixed for 20 min at 4°C in cold BD fixation/permeabilizationTM solution (BD Bioscience). Cells were washed twice in BD Perm/WashTM Buffer (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... LPLs were then stained with the following transcription factor antibodies for 30 min at 4°C: RORψt (Q31-378, AF647, BD) and Foxp3 (FJK-16s ...
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bioRxiv - Molecular Biology 2023Quote: ... then at 4 °C for at least 30 min before collection with a flow cytometer (BD Biosciences C6 Acuri). 20-30,000 events were collected in specific PE-A and PE-H gates for further deconvolution or data analysis using FlowJo vX 10.0.7r2 (Tree Star ...
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bioRxiv - Immunology 2023Quote: ... with blockade of cytokine secretion being achieved by simultaneous treatment with 4 µl of GolgiStop (# 51-2092KZ, BD Biosciences) for every 6 ml of cell culture for the last 3 hours ...
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bioRxiv - Immunology 2024Quote: ... 4°C for 10 min and binding was analyzed by flow cytometer FACS LSRFortessaTM (BD Bioscience, Franklin Lakes, NJ) using Yellow/Green Ex 561 nm Em 586/15 laser for the detection of antibodies ...
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bioRxiv - Genetics 2024Quote: ... Following blocking (5% milk/BSA) membranes were incubated with primary antibody overnight at 4°C (BIP [1:40,000 BD Transduction 610979] ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 4 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (400 μg; Becton, Dickinson and Company, Sparks, MD, USA) as described previously [21-23] ...
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bioRxiv - Neuroscience 2024Quote: ... 0.78 mM EDTA) and incubated for 30 min at 4 °C with FACS buffer containing Fc block (BD Biosciences) and primary antibody mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Single cells were stained with the appropriate antibodies to surface markers at 4°C for 30 minutes in the dark.The following fluorescent dye-labeled antibodies purchased from BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... HUVECs grown for indicated time periods on 0.1% gelatin-coated glass chamber slides (4- or 8-well; BD Falcon) in FA-supplemented or control media were washed once in PBS and fixed for 10 min on ice with 4% PFA ...
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bioRxiv - Neuroscience 2024Quote: ... in flotation for 1 h at room temperature and then overnight at 4°C with the mouse antibody that recognizes protein GM130 (Cat# A-610822, RRID:AB_398141, BD Biosciences), followed by incubation for 2 h at room temperature with Alexa Fluor 546-conjugated goat anti-mouse secondary antibodies (Cat#A-11030 ...
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bioRxiv - Neuroscience 2024Quote: The full 750-800µL suspension was loaded at 4°C into a FACSAria™ Fusion flow cytometer (BD Biosciences) with a 70 µm nostril running regular 1X PBS at the Flow Cytometry Core Facility of the Molecular Biology Department at Princeton ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour and then exposed overnight at 4°C to the primary antibody (ZO-1, 610966, BD transduction). Following three washes in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen tissue sections were fixed in 4% formaldehyde and immunostained with the following antibodies: CD31 (BD Pharmingen, 1:50), ER-TR7 (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2020Quote: ... and 3-Iodo-L-tyrosine-treated B cells were labeled by using the Annexin V Fitc Apoptosis detection kit (BD, Heidelberg, Germany) according to manufacturer`s instructions ...
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... N2a-ssGT cells were imaged for GFP fluorescence using CytationTM 3 Cell Imaging Multi-Mode Reader (BioTekTM) and flow cytometry analysis by Accuri® C6 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and the data was analyzed using FlowJo software (BD Biosciences, RRID:SCR_008520). All listed antibodies for flow cytometry are from Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was weighed then homogenized for 3 minutes in a bullet blender and serum was added to a serum separator tube (BD Microtainer: 365967) before being spun down and diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and VC3ai cleavage by caspase-3/7 was assessed based on fluorescence emission at 530 nm using a BD LSR Fortessa™ Cell Analyzer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... Tracheal intubation was performed after sevoflurane exposure (5%, 3 minutes) using a 20-gauge intravenous catheter (REF 382434; BD Angiocath Plus, Singapore). To ensure proper positioning of the catheter so as to avoid one- lung ventilation and gas leakage during ventilation ...
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bioRxiv - Bioengineering 2024Quote: The expression of the exhaustion markers PD-1 and TIM-3 was evaluated using anti-PD1 (cat. 560795, BD Biosciences, BD PharMingen) and anti-TIM3 (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... eBioscience] for 45 min at 4°C together with different additional antibodies for immunophenotyping (including 1:10 anti-CD103-FITC [clone Ber-ACT8, BD Biosciences] ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained at 4°C for 15 minutes with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen), a BV650-anti-Human-CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... then cells were collected and 60 μg of sonicated chromatin was incubated overnight at 4°C with 20 μl of the anti-BrdU antibody (347580 Pure BD). Then ...
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bioRxiv - Neuroscience 2022Quote: ... and the following antibody mixture in PBS at 4°C for 30 minutes: BV421 Rat Anti-CD11b (BD Biosciences 562605), BV510 Hamster Anti-Mouse TCR β Chain (BD Biosciences 563221) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibody in incubation buffer overnight at 4°C with mouse anti-CtBP2 antibody (1:50, BD Transduction ...
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bioRxiv - Immunology 2021Quote: ... for 4 h at 37°C in the presence of both Brefeldin A (GolgiPlug) and Monensin (GolgiStop, both BD Biosciences). As GolgiPlug and GolgiStop were used together ...