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Citations for Becton, Dickinson and Company :
2251 - 2300 of 3199 citations for 4 nitro 5 ethyl 2 methylpyridine n oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Then the syndecan-4 expression on HUVECs was measured by flow cytometry with a BD Biosciences LSR II (BD Biosciences, CA, USA).
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bioRxiv - Immunology 2022Quote: ... at 4°C for 5 min before surface staining with a cocktail of the following antibodies for 20 min at 4°C: CD3 V450 (Clone 17A2) (BD Cat. 561389), CD4 BV750 (Clone H129.19 ...
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bioRxiv - Microbiology 2019Quote: ... The resulting solutions were diluted (10-1 - 10-4) and 10 µl were seeded in plates containing potato dextrose agar (PDA) (BD-Difco®) and Sabouraud agar (SBA ...
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bioRxiv - Cancer Biology 2020Quote: Plasma serum was collected from 4-6 drops of peripheral blood obtained from NSGS mice via submandibular bleeding using Microtainer Collection Tubes (BD Microtainer, # 365967). Concentrations of 29 cytokines/chemokines were measured in 2-4 biological replicates using MILLIPLEX Map Human Cytokine/Chemokine Magnetic Bead Panel (HCYTMAG-60K-PX29 ...
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bioRxiv - Neuroscience 2021Quote: ... A 1ml astrocyte cell suspension of 4×104 cells/ml was plated in three chambers of four-chamber glass slides (BD Falcon, USA) coated with poly-D-lysine ...
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bioRxiv - Immunology 2020Quote: ... and cells were incubated for 4 h at 37 °C in the presence of BD GolgiPlug™ protein transport inhibitor (BD Biosciences). Cells were then labeled with anti-CD8a antibody and a dead cell marker (Live/Dead Fixable Aqua Dead Cell Stain Kit) ...
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bioRxiv - Immunology 2020Quote: ... in house production), 10 μg/mL α-IL-4 (cl. 11B11, in house production) and 10 ng/mL IL-12 (BD Pharmingen) and cultured on goat α-hamster IgG (MP Biochemicals ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended in PBS/5%FBS then fixed in an equal volume of 1% paraformaldehyde and stored in the dark at 4°C prior to analysis on a BD LSR FortessaTM or LSR2 flow cytometer (BD Biosciences, UK). Data were analysed using FlowJo software (FlowJo LLC ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed at 4 °C for 20 min and washed in permeabilization buffer from the Fix/Perm kit (BD Biosciences, 554714). Antibodies against intracellular targets were diluted in permeabilization buffer and cells were incubated at 4 °C for 30 min followed by another wash prior to assessment with BD LSR Fortessa SORP cytometer ...
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bioRxiv - Immunology 2022Quote: ... The Fc cell receptors were then blocked for 10 min at 4°C with an anti-CD16/CD32 antibody (10 μg/mL: BD Biosciences 553142) and the cells were resuspended in 25 μL of staining buffer ...
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bioRxiv - Physiology 2022Quote: ... Venous peripheral blood (4-6 ml for adults, 1- 3 mL for children and infants) was collected on-site on EDTA (BD vacutainer©) and immediately stored at 4-8°C until use ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Immunology 2023Quote: ... PE-cy7-conjugated anti-TNF-α (Clone MP6-XT22, 1:800), and APC-conjugated anti-IL-4 (Clone 11B11, 1:100) (BD Biosciences). All stained samples were acquired using an LSR-Fortessa flow cytometer or Symphony A3 (Becton Dickinson ...
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bioRxiv - Genetics 2023Quote: ... Cells were collected every day up to day 4 and were stained with FITC-conjugated IgG1 (1:200, A85-1, BD Pharmigen, 553443) and PE-Cyanine5-conjugated B220 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... all cells were harvested from the culture plates and the cell Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). Then ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 10 ng/mL anti-CD3 mAb at 4°C overnight (Purified NA/LE mouse anti-human CD3, BD Cat# 557052). Three-fold serial dilutions of either IL-7 or a test compound were added to the cells and incubated for 4 days at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cells were washed once in FACS buffer before fixation for 20 min at 4°C using the BD Cytofix/Cytoperm solution and permeabilization using BD Perm/Wash™ buffer (BD Biosciences). Permeabilized cells were incubated for 30 min with antibodies (diluted 1:200 in BD Perm/Wash ...
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bioRxiv - Genomics 2023Quote: Whole blood aliquots at a volume of four (4) milliliters were collected from participants using sterile Vacutainer blood collection kits (BD Vacutainer®) and subsequently biobanked at −80°C for DNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... treatment was performed at 100μg/ml concentration in dark at 4°C for an hour and analyzed by FACS Calibur flow cytometer and processed using Cell Quest software (BD Biosciences, USA).
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL medium containing 1 × 106 luciferase-expressing MCF10AER/vSrc cells (control or 4-OHT transformed, with or without NLS-catalase expression) were mixed with 75 μL Matrigel matrix (BD Bioscience, #356237) containing 10 μM 4-OHT and injected into the inguinal mammary fat pad of the female mice at 6 to 8 weeks of age ...
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bioRxiv - Immunology 2023Quote: Splenocytes-or BM-derived cells were resuspended in sPBS and the Fc receptors were blocked for 10 min at 4°C with 10 μg/mL anti-CD16/CD32 antibodies (BD Biosciences 553142). After blocking ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Immunology 2023Quote: ... Blood was collected via submandibular bleeds with goldenrod 4 mm lancets collected into sample tubes coated with K2 EDTA (BD Ref# 365974) and 60 μL of blood was lysed with 500 uL of ACK lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was carried out for 30 minutes at 4°C with the following fluorochrome-labeled anti-mouse Abs: APC/Cy7-CD45 (BD Bioscience, #557659), PE/Cy5-B220 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... followed by two washes with the permeabilization buffer before incubation with 4 μg/ml Alexa Fluor® 647 Mouse anti-Phospho-NF-κB p65 (pS529) (BD Biosciences) for 45 min.
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bioRxiv - Bioengineering 2024Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cels were fixe with 4% PFA and stained with Rb anti-HA for NDST1 (CST 3724S) and Ms anti-GM130 (BD biosciences 610823) for Golgi followed by secondary antibodies staining with anti-Rb-A550 and anti-Ms-A647 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were blocked with Superblock blocking buffer in PBS/ 0.1% Triton-X for 1 h and then incubated with the following primary antibodies overnight at 4 °C: α-CD11b (1:100; BD Biosciences) and α-Iba1 (1:200 ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Biophysics 2021Quote: ... HUVECs were plated on coverslips coated with ∼50 μg/ml (5 mg/cm2) collagen from rat tail (BD Biosciences, 354236). Y-27632 (Y100500 ...
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bioRxiv - Cancer Biology 2021Quote: ... γδ T cells were washed twice in cold FACS buffer (PBS, 5 mM EDTA, 1% bovine serum antigen) and stained with 1:20 anti-CD3-PerCP-Cy5.5 (BD Biosciences), 1:20 anti-TCRγδ-PE (BD Bioscience) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 200 μL 1x binding buffer with or without 5 μL Annexin V (BD Biosciences, Billerica, MA). After 15 min incubation ...
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bioRxiv - Microbiology 2020Quote: ... Experimental starters were prepared from frozen master stocks struck on 5% Columbia blood agar plates (Biomerieux Ref# 43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50 mM HEPES (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 100 μl PBS/5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: All strains were grown while shaking at 200 rpm in 5 mL of Luria-Bertani (LB) broth (BD Diagnostic Systems) for 16 h at 37 ℃ unless otherwise stated (Table S1).
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bioRxiv - Microbiology 2022Quote: ... were revived from freezer stocks on Columbia agar plates supplemented with 5% (v/v) sheep blood by incubating them anaerobically for 48 h at 37 ºC (BD GasPak EZ Anaerobe Gas Generating Pouch System ...
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bioRxiv - Neuroscience 2021Quote: ... the cell pellet was suspended in alpha minimum essential medium (MEM) with 5% fetal bovine serum (FBS) and 0.6% glucose and filtered with a 40-μm cell strainer (BD Falcon). After centrifugation (200 × g ...
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bioRxiv - Immunology 2021Quote: ... 5 µL of each aspirate was lysed with 45 µL of 1x red blood cell (RBC) lysis buffer (BD Bioscience) and the optical density (OD ...
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bioRxiv - Immunology 2020Quote: NK cells were co-cultured for 5 hours with K562 or GSCs target cells at an optimized effector:target ratio of 5:1 together with CD107a PE-CF594 (BD Biosciences), monensin (BD GolgiStopTM ...
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bioRxiv - Physiology 2019Quote: All flies were raised in vials on sterile standard cornmeal medium containing 5% inactivated yeast (BD Bacto™ yeast extract), 8.6% cornmeal (Mühle Schlingemann ...
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bioRxiv - Microbiology 2019Quote: ... Selected isolates were revived from −80 °C on Columbia agar plates with 5% sheep blood and incubated under anaerobic conditions (BD GasPak™ EZ Anaerobe Gas Generating Pouch System ...
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bioRxiv - Immunology 2020Quote: ... CLTX-CAR T cells were incubated with GBM cells (E:T=1:1) for 5 h in the presence of CD107a antibody and GolgistopTM protein transport inhibitor (BD Biosciences). To test for CAR T cell killing potency ...
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bioRxiv - Cell Biology 2019Quote: ... cells were grown for 5 days before being sorted based on green fluorescence using a fluorescence-activated cell sorter (FACS; FACSAria IIu, BD). AICSDP-1:PXN-EGFP was a gift from The Allen Institute for Cell Science (Addgene plasmid 87420).
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 561018 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies (CD45-APC BD Biosciences 559864 ...
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bioRxiv - Cancer Biology 2021Quote: 1.5×106 pCAF2s expressing shCtl or shPYCR1 and 5×105 MCF10DCIS.com in 200 µl 50% growth factor reduced phenol red free Matrigel (BD Biosciences) in PBS were injected subcutaneously into the flank of 8 week old female BALB/c nude mice (Charles River) ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 x 106 cells/ml) and immediately sorted using a FACS Aria cell sorter (BD Biosciences). Subsequently ...