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Citations for Becton, Dickinson and Company :
2001 - 2050 of 7602 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Control dCas9 or DAAP1 targeting dCas9+sgRNA392-411 trophozoites were chilled for 20 minutes on ice and then loaded into microfluidic devices placed in a GasPak EZ anaerobic pouch (BD Scientific) at 37 °C for 1 h to allow re-attachment ...
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bioRxiv - Cell Biology 2021Quote: ... The percentage of GFP-positive cells among the mCherry-positive cells was determined 48 h after I-SceI transfection using a LSRFortessa X-20 (BD Bioscience) using BD FACSDiva Software v8.0.1 ...
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bioRxiv - Systems Biology 2020Quote: ... Both dried sides of PDMS and glass substrates underwent with plasma cleaning by hand-held corona treater (Electro-Technic, BD- 20) for 2 minutes ...
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bioRxiv - Cell Biology 2021Quote: Trophozoites were chilled for 20 minutes on ice and then loaded into microfluidic devices placed in a GasPak EZ anaerobic pouch (BD Scientific) at 37 °C for 1 hour to allow re-attachment ...
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bioRxiv - Cell Biology 2021Quote: ... freshly isolated primary HCs or cultured HCs collected after ploidy transition were fixed at 2×106/ml in 75% ethanol overnight at −20°C.Cells were then incubated with 500 μl of PI/Rnase Staining Buffer (550825, BD Pharmingen, USA) at room temperature for 15 min and analyzed by flow cytometry.
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bioRxiv - Cancer Biology 2021Quote: ... aliquots were mixed gently while adding annexin-binding buffer on ice and analyzed by flow cytometry (LSRFortessa X-20; BD Biosciences). Cell cycle was assessed using the same cells and conditions described above with Click-iT EdU Alexa Flour 647 Flow Cytometry Assay (ThermoFisher ...
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bioRxiv - Genetics 2022Quote: Calculation of the proportion of apoptotic cells after treatment with 20-HE with Annexin-PI staining was conducted using FlowJo V10 software (BD, Lifesciences). All the results for fluorescence-based estimation of caspase-3 activity and the fold change relative expression of HzCaspase-3 and HzCaspase-8 were graphed and analyzed by unpaired t- test for individual comparisons between the treated and untreated cells ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were transferred to a V bottom 96-well plate to run flow cytometry analysis on LSRFortessa X-20 (BD Bioscience). mScarlet / Clover ratio was calculated as Q2/(Q2+Q4 ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry was performed as previously described (63) using either a BD LSR Fortessa or a BD X-20 BD (BD Biosciences). Representative flow plots in the Figures illustrate the distribution of the target population ...
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were later suspended in FACS buffer (98% PBS, 2% FBS) and analyzed by flow cytometry (BD LSR-Fortessa X-20). After the sample data was acquired ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were then consecutively stained with FCRLs monoclonal rat antibody (Butovsky et al., 2014) (30 min), goat anti-rat APC antibody (Biogegend, # 405407) (20 min) and Cd11b PeCy7 antibody (BD, #553142) (20 min ...
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bioRxiv - Neuroscience 2021Quote: ... for 20 min at 37 ÅãC and obtained single-cell suspensions with narrow-bore pipettes and a 40 μm strainer (BD-Falcon), plating the cells in 24 well low attachment plates (Corning) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Three washes were performed using PBE and then cells were resuspended in PBE and run on LSRFortessa X-20 Flow Cytometer (BD Biosciences). Analysis was performed using FlowJo software (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... aliquots were mixed gently while adding annexin-binding buffer on ice and analyzed by flow cytometry (LSRFortessa X-20; BD Biosciences). Cell cycle was assessed using the same cells and conditions described above with Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse xenograft tumors were generated by injecting 106 4T1 cells resuspended in 20% collagen I (BD Biosciences, Franklin Lakes, NJ, USA) in PBS into the lower-left mammary gland of 8-week-old BALB/c female mice ...
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bioRxiv - Cell Biology 2022Quote: ... Non-adherent cells transduced with PMG based vectors were typically sorted for top 5-20% ZS-green expressing cells once on a FACSAria III (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: Cell were incubated 20 min in culture media completed with a staining solution composed of MitoTracker Green FM (100 nM) (BD Horizon), TMRE (ultra pure ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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bioRxiv - Immunology 2020Quote: ... Samples from patients 01–09 were measured on a LSRFortessa flow cytometer and samples from patients 10–20 on a FACSymphony (both BD Biosciences).
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bioRxiv - Cancer Biology 2021Quote: ... and NLF-shNT or -shPRELP (0.5×106 cells) were suspended in 20 µl of cell culture medium and growth-factor-reduced Matrigel (catalog no. 356230; BD Biosciences) 1:1 and co-injected into the liver of six-week old athymic nude mice (n=5 per condition).
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bioRxiv - Immunology 2020Quote: ... cells were harvested and either resorted based on viability for Fluidigm gene expression analysis or were immediately fixed for 20 min at room temperature (100μL, BD cytofix/cytoperm) for Ki67/DAPI analysis or puromycin incorporation assays ...
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bioRxiv - Immunology 2020Quote: Cell-free supernatants were stored at –20°C until their analysis for chemokine/cytokine content with a multiplexed bead-based immunoassay (BD(tm) Cytometric Bead Array) ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibodies for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer and once with staining buffer before analysis using BD Symphony Flow Cytometer ...
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bioRxiv - Immunology 2020Quote: ... plasma fraction was harvested after 20 minutes centrifugation at 1700 x g of blood collected in BD Vacutainer CPT tubes (BD, #362753). Plasma samples were treated by solvent/detergent treatment with a final concentration of 1% Triton X-100 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The permeabilized cells were stained with ICS antibody cocktail (Suppl. Table 2) for 20 min at room temperature in 1X-perm/wash buffer (BD Biosciences). Cells were then washed twice with perm/wash buffer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 5e6 spleen cells were restimulated in vitro with 5e6 irradiated ERAAP KO spleen cells and 20 U/ml of recombinant human IL-2 (BD Biosciences). After 6 days ...
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bioRxiv - Microbiology 2022Quote: ... which is equipped with a solid-state blue laser (488 nm, 20-mW) and a He/Ne ion red laser (633-nm, 17-mW) with BD FACSDiva software (BD Biosciences). The same excitation and emission parameters as used during flow cytometry were also used for cell sorting.
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bioRxiv - Microbiology 2022Quote: ... equipped with a solid-state blue laser (488 nm, 20-mW) and a He/Ne ion red laser (633-nm, 17-mW) with BD FACSDiva software (BD Biosciences). The blue laser was used to excite Sybr Green I ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 300 μL FACS Binding Buffer and analysed using a BD LSRFortessa X-20 Flow Cytometer (BD Biosciences). Data were evaluated using FlowJo version 10.
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bioRxiv - Developmental Biology 2022Quote: ... PAX8 and BrdU incorporated cells (BrdU+) data were obtained and processed using an LSR-Fortessa X-20 flow cytometer and FACSDiva software (BD Biosciences). Unstained cells and isotype controls were included in all experiments ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The electroporated transgenic larvae were introduced in the chip by sucking them up into a PFT tube plugged to a metallic needle (Mircolance #20, 302200, BD, USA) connected to a plastic syringe (Luer Plastipak ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... data were acquired on BD LSR Fortessa X-20 Flow cytometer using BD FACSDiva software (BD Biosciences, San Jose, CA, USA) and analyzed on FlowJo software by BD Biosciences (https://www.flowjo.com/).
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bioRxiv - Molecular Biology 2024Quote: ... in PBS at 37°C for 30 min and analyzed with a FACSCalibur or a LSRFortessa X-20 machines (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... Stained cells were washed twice and data were acquired on a BD LSR Fortessa X-20 (BD Biosciences, San Jose, CA), using FACS Diva Software v8 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with anti-CD16/32 APC-Fire750 and anti-CD64 PECy7 for 15 min on ice before the addition of the rest of the fluorophore-conjugated antibodies were added on ice for 20 min in Brilliant Stain Buffer (BD Biosciences). For GATA6 staining ...
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Neuroscience 2022Quote: ... Syn1 [a mouse monoclonal antibody that recognises residues 91-99 from the NAC region of human α-synuclein (20)] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... Anti-Mouse) and propidium iodide as per the standard flow cytometry protocol and analyzed thereafter (LSRFortessa™ X-20 cell analyzer, BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with perm buffer and resuspended in PBS 1X and acquired on a BD LSRFortessa X-20 (BD Science). Flow data were analysed using FlowJo software (version 10.0.8r1 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for a minimum of 20 minutes with 2% paraformaldehyde and acquired immediately using a FACS Symphony A3 (BD Biosciences). Where intracellular staining was performed ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by two PBS-wash steps and secondary antibody staining and analysis on an LSRFortessa X-20 flow cytometer (BD Biosciences), which was also used for all downstream flow cytometry.
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bioRxiv - Physiology 2023Quote: ... Images were acquired using a confocal microscope (Zeiss Axiovert 200, objectives ×20, ×40, and ×100) equipped with a CARV II confocal imager (BD Biosciences) (48) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed and fixed in formalin/PBS for analysis with BD LSRFortessaTM X-20 and BD FACs Diva Software (BD Biosciences) or Cytek® Aurora and SpectroFlo® software (Cytek Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then dissociated and washed two times with D-PBS before fixing and permeabilisation for 20 minutes (554714 Cytofix/Cytopearm, BD Biosciences). Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized with BD Fix/Perm for 20 min at room temperature (RT) and stained intracellularly with antibodies in 1x Perm buffer (BD Biosciences): anti-IFN-γ (1:100 dilution ...
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bioRxiv - Immunology 2023Quote: ... Flow cytometry was performed using mock infected LOs as a negative control and gates were set to exclude the dead cells (Zombie UV stained) using the LSR Fortessa X-20 (BD Biosciences). All experiments were done a minimum of three separate times in three technical replicates ...