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Citations for Becton, Dickinson and Company :
2251 - 2300 of 7602 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... N2a-ssGT cells were imaged for GFP fluorescence using CytationTM 3 Cell Imaging Multi-Mode Reader (BioTekTM) and flow cytometry analysis by Accuri® C6 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and the data was analyzed using FlowJo software (BD Biosciences, RRID:SCR_008520). All listed antibodies for flow cytometry are from Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was weighed then homogenized for 3 minutes in a bullet blender and serum was added to a serum separator tube (BD Microtainer: 365967) before being spun down and diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and VC3ai cleavage by caspase-3/7 was assessed based on fluorescence emission at 530 nm using a BD LSR Fortessa™ Cell Analyzer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Cell Biology 2019Quote: ... The inner well of assembled stretch chambers was ozone-activated for 30 s via corona arc-discharge (BD-20, Electro-Technic Products Inc.) and coated with 50 µg/ml bovine plasma fibronectin (F1141 ...
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bioRxiv - Cell Biology 2020Quote: ... flow cytometry was performed using a BD LSRFortessa X-20 or BD LSR-II analytical flow cytometer (BD Biosciences, San Jose, CA, USA) and FCS files were analysed using FlowJo version 10 (Tree Star Ashland ...
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bioRxiv - Biochemistry 2020Quote: ... DsRed positive cells and the arithmetic mean fluorescence intensity (‘MFI’) of all cells in the PE-channel were also quantified using flow cytometry (BD Fortessa X-20). Microscopy images were acquired using a Zeiss AxioObserver instrument (10X/0.25 NA or 20X/0.4 NA objective).
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bioRxiv - Cell Biology 2020Quote: ... the DRG neurons were mechanically separated by 10 to 20 cycles of pipetting using a small diameter Pasteur pipette and filtered through a 40 μm cell strainer (BD Falcon, United States). The isolated neurons were placed on 12-mm diameter coverslips (Matsunami ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Single cell suspensions of enzyme dissociated pancreatic islets were stained for 30 min on ice and subsequently analyzed on a Fortessa X-20 flow cytometer (BD Biosciences, CA, USA).
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bioRxiv - Immunology 2020Quote: ... bone marrow-derived cells were filtered through sterile polystyrene syringes (70–100 μm) and divided into 10 Petri dishes (100 × 20 mm; BD, Franklin Lakes, USA) supplemented with murine M-CSF (20 ηg/mL ...
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bioRxiv - Genomics 2020Quote: ... [9] containing 2 μg/mL DAPI and 40 μg/mL Ribonuclease A and filtered through a CellTrics® 20-μm nylon mesh filter (Partec) just before flow sorting on an InFlux™ flow cytometer (BD Biosciences) equipped with UV (355 nm ...
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bioRxiv - Immunology 2020Quote: Mice were injected with 15-20 µg of anti-CD8α-AF488 (eBioscience, clone 53-6.7) or anti-CD8α-AF647 (BD Biosciences, clone 53-6.7) with/without EpCAM-AF647 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The labeled cells were cultured in complete RPMI-1640 medium plus IL-2 (20 units/ml) or IL-2 and anti-CD3 antibody (1μg/ml) (BD Pharmingen (San Diego, CA), or alternatively plus IL-4 (4ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells from this population were then singled-out in 96-well plates in conditioned medium containing 20% FBS (using a BD FACSAriaTM cell sorter) and individual clones were allowed to expand before genomic DNA was again extracted ...
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bioRxiv - Plant Biology 2024Quote: ... The strain was revived from a 20 % glycerol conidial suspension stored at -80°C and then maintained on potato dextrose agar media (PDA; BD Difco Laboratories, France) at 20°C in dark conditions ...
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bioRxiv - Cell Biology 2024Quote: ... Dissociated cells were then labelled with live and dead dye before live cell sorting using a BD LSR FORTESSA™ X-20 Cell Analyzer (BD Biosciences, USA). Single cells were captured and barcoded using 10x Chromium platform and the sequencing libraries were prepared following manufacturer’s instructions using dual index Chromium Next GEM G Single Cell 3’ kit v3.1 (10xGemonics ...
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bioRxiv - Microbiology 2019Quote: ... Culture of 10ul from dilutions 1:10-1 - 1:10-8 was performed on CHROMagar Orientation (BD) and ESBL Brilliance agar (Oxoid) ...
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bioRxiv - Cancer Biology 2024Quote: ... and CAPAN-1 cells diluted in 200 μl of a 1:1 mixture of Matrigel (BD Biosciences) and PBS (Corning) ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained at 4°C for 15 minutes with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen), a BV650-anti-Human-CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Neuroscience 2022Quote: ... and the following antibody mixture in PBS at 4°C for 30 minutes: BV421 Rat Anti-CD11b (BD Biosciences 562605), BV510 Hamster Anti-Mouse TCR β Chain (BD Biosciences 563221) ...
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bioRxiv - Immunology 2021Quote: ... for 4 h at 37°C in the presence of both Brefeldin A (GolgiPlug) and Monensin (GolgiStop, both BD Biosciences). As GolgiPlug and GolgiStop were used together ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... followed by 200 μL FACS buffer before resuspending the cells in FACS buffer for acquisition in a high-throughput sampler on a 4-laser LSR Fortessa (BD), at a flow rate of 1 μL/s.
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bioRxiv - Immunology 2021Quote: ... cells from tumors were cultured ex-vivo from tumors for 4 hours in the presence of Golgi Plug (BD Biosciences) prior to cell surface and intracellular staining.
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bioRxiv - Immunology 2020Quote: ... for 2 h and 4 h respectively at 37°C in 5% CO2 and exposed to brefeldin A (BD Biosciences) as described in previous studies ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed twice (PBS) and fixed with 500 μl of 4% PFA and analysed by flow cytometry (BD Bioscience). A total of 10,000 events were acquired and data were analysed using FlowJo software version 10.6.2.
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bioRxiv - Neuroscience 2019Quote: ... mice received a single treatment with an antibody against Ly6G (α-Ly6G; IP 4 mg/kg; BD Bioscience No.: 561005) or with an isotype control antibody (Iso-Ctr ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with the following antibodies for 30 min at 4°C: CD38 (Cat#342371; RRID: AB_400453, BD Biosciences), lineage cocktail (CD3/14/16/19/20/56 ...
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bioRxiv - Cancer Biology 2022Quote: Wells with the highest proportion of target barcodes were revived into a 6 well plate and grown for 4 days before being single-cell sorted by BD FACSAria II into 96 well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary or conjugated antibodies were incubated with the cells overnight at 4°C in Perm/Wash buffer (554714, BD Biosciences) containing 4% FBS and secondary antibodies for 45 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation for 25 min at 4° C with the following mouse specific antibodies to delineate subpopulations: CD24-PE (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... The cells (2-4 x 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878 ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... and stained with the following cocktail for 30 minutes at 4°C in the dark to enable delineation of mature mononuclear phagocytes: Anti-CD45 PE-Cy7 (BD), anti-HLA-DR eFluor 450 (eBioscience) ...
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bioRxiv - Biophysics 2020Quote: ... The samples were kept in the dark at 4°C until analyzed using a LSR II flow cytometer (BD Biosciences). Hoechst 33342 and Pyronin Y staining were measured with a UV (350 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... generated by hanging drops culturing of ESCs (1,000 cells per drop) were collected after 4 days and embedded in growth-factor-restricted Matrigel (MTG; BD Biosciences); 50µl MTG drops (containing around 20 EBs ...
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bioRxiv - Cell Biology 2020Quote: ... intranuclear staining was performed at 4 °C overnight in the dark using the FITC anti–human Ki-67 antibody (BD) diluted in Perm/Wash ...
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bioRxiv - Immunology 2019Quote: Cells were analysed using 4- to 6-color direct immunofluorescence staining on a BD LSR II flow cytometer (BD Biosciences). Antibodies used were ...