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Citations for Becton, Dickinson and Company :
1951 - 2000 of 2278 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
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bioRxiv - Biophysics 2020Quote: ... After incubation cell free supernatant was collected and subjected to sandwich ELISA using IL-2 specific antibody (BD biosciences, CA, USA) as per manufacturer instruction.
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bioRxiv - Cancer Biology 2021Quote: ... The spheroids were then embedded in a collagen l/Matrigel gel mix at a concentration of approximately 4 mg/ml collagen I and 2 mg/ml Matrigel (BD Bioscience) in 24-well glass-bottomed cell culture plates (MatTek ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions (1.5 x 104 cells per ml) were plated in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 100μl of this mix were added per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were co-stained with 4′,6-diamidino-2-phenylindole (DAPI) to measure DNA content and analyzed with a BD LSRFortessa flow cytometer (BD Biosciences) and FlowJo software (TreeStar).
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Pacific Blue; BD Biosciences), anti-CD4 (L200 ...
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bioRxiv - Bioengineering 2021Quote: ... and cells collected from the wells were washed and stained with propidium iodide (2 µg/ml) to distinguish live from dead and run on a flow cytometer (BD FACSCelesta).
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bioRxiv - Immunology 2021Quote: ... were cultured with 5E5 of YAC-1 target cells (2:1 ratio) in the presence of anti-mouse CD107a-BV421 antibody (564347, BD Biosciences) (1:100 ...
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bioRxiv - Immunology 2021Quote: ... 1×105 Tconv cells were co-cultured with Treg cells at different ratios in the presence of 2×105 irradiated APCs and 1 µg/ml soluble anti-CD3 antibody (554829, BD Biosciences). The cells were cultured in T-cell complete media which consists of RPMI 1640 medium supplemented with 50 U/ml penicillin ...
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bioRxiv - Cell Biology 2020Quote: ... Clonal HEK293 cells stably expressing pCRE-DD-zsGreen1 and dCas9-BFP-KRAB were generated by lentiviral transduction and fluorescence activated cell sorting for GFP and BFP on a BD FACS Aria 2 (BD Biosciences). Individual clones were expanded and tested for 1 ...
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bioRxiv - Immunology 2021Quote: ... For intracellular staining cells were fixed and permeabilized with 2% paraformaldehyde and 70% methanol or with BD Cytofix/ Cytoperm buffer (BD Biosciences) and permeabilization buffer (eBioscience ...
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bioRxiv - Cell Biology 2020Quote: Protein synthesis of Syndecan-1 was shown by immunocytochemistry using a polyclonal antibody against murine Syndecan-1 (CD138) clone 281-2 (1:50, rat anti-mouse, BD Pharmingen) in co-cultured cells after fixation with ice-cold 99% methanol and heparitinase III (2.4mIU/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 partly overlapping holes were punched out by using a 16-gauge Jamshidi bone marrow biopsy needle (BD Biosciences., NJ, USA). The edges of the holes were trimmed with a surgical scalpel and the bottom of the defect was curetted to expose the bleeding subchondral bone ...
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bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
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bioRxiv - Molecular Biology 2020Quote: The SARS-CoV-2 S gene (Genbank accession no. NC_045512.2) was synthesized and cloned into a baculovirus shuttle vector pAcgp67B (BD Biosciences, CA, USA) using Gibson assembly ...
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bioRxiv - Immunology 2022Quote: ... The stained cells were washed and resuspended in PBS containing 2% FBS before being strained through a 70-μm cell mesh filter (BD Biosciences). SARS-CoV-2 spike-specific single B cells were gated as CD19+CD27+CD3-CD14-CD56-IgM-IgD-IgG+Spike+ and sorted into 96-well PCR plates containing 10 μL of RNAase-inhibiting RT-PCR catch buffer (1M Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACSAria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Immunology 2021Quote: Cultured T cells were stimulated with the cognate SARS-CoV-2 OPPs (ORF1ab-C, ORF3a, N, S, nsp12) and incubated for 4 hours in the presence of Golgiplug (BD Biosciences). Following stimulation ...
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bioRxiv - Immunology 2021Quote: ... and stained for 20 minutes at 4°C with 50 μl of antibody cocktail containing subset-specific antibodies (Supplementary Table 2) diluted in Brilliant Stain Buffer (BD Biosciences). Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Immunology 2020Quote: ... the PBMCs were stimulated with pooled or individual peptides at a final concentration of 10 μg/mL per individual peptide for 1 h in the presence of 2 μg/mL monoclonal antibodies against human CD28 (BD Pharmingen) and CD49d (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 hr at 4°C with extracellular domain anti-GluN1 antibody (BD biosciences; 2 μg ml−1) followed by two washes with ice cold phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cancer cells were washed with ice-cold PBS with 2% FBS and stained with Near-IR Live/Dead (BD Biosciences) on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... xenograft models were generated by injecting ES2-shARID1A cells [2 × 106 cells mixed 1:1 (v/v) with Matrigel (BD Biosciences)] subcutaneously ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were grown to logarithmic phase in SC 2% glucose medium consisting of 0.17% yeast nitrogen base (BD Difco™, 233520), 5% (NH4)2SO4 supplemented with 30 mg/L of all amino acids (except 80 mg/L histidine ...
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bioRxiv - Genomics 2021Quote: ... hPBMCs were resuspended in 1x PBS + 2% FBS and stained with a FITC-conjugated Mouse Anti-Human CD45 Antibody (Clone HI30, BD Biosciences) for 25 min on ice ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
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bioRxiv - Microbiology 2021Quote: ... The stained cells were washed and resuspended in PBS containing 2% FBS before being strained through a 70-μm cell mesh filter (BD Biosciences). RBD-specific single B cells were gated as CD19+CD27+CD3−CD14− CD56−IgM−IgD−IgG+RBD+ and sorted into 96-well PCR plates containing 10 μL of RNAase-inhibiting RT-PCR catch buffer (1M Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: Candida strains (Supplementary Table S1) were routinely grown at 30°C in yeast peptone dextrose (YPD) media (2% peptone (BD Bacto), 2% D-glucose (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Cells were fixed in 2% paraformaldehyde and GFP fluorescence was measured using the BD Accuri™ C6 flow cytometer (BD Biosciences). Flow sorting was performed on unfixed cells the FACS Aria III (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... For both ACHN and CAKI-1 cell lines 2×106 cells were diluted in 50 µl of PBS and 50 µl of Matrigel (BD Biosciences) and were injected subcutaneously into the right and left flank of each mouse ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked for 10 min in ice-cold PBS+2% heat-inactivated FBS (HI-FBS) containing anti-CD16/CD32 (BD Biosciences) and rat-γ-globulin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... APC-R700 rat anti-mouse IL-2 (Cat No.-565186) and PE-CF594-rat anti-mouse IFN-γ were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... into FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and analyzed with a FACS Aria flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACS Aria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... samples of 1×106 per mL pooled CAR T cells with 2×106 per mL Nalm 6 were centrifuged at 300 × g for 2 min in 96-well flat-bottom plates and incubated in HTCM with 1x Brefeldin-A/GolgiPlug (BDBiosciences #555029), 1x Monensin/GolgiStop (BD Biosciences #554724) ...
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bioRxiv - Molecular Biology 2022Quote: ... CD4+ T cells or MT-2 cells were activated with media containing 50 ng/mL anti-CD3 (eBioscience) and 100 ng/mL anti-CD28 (BD Biosciences) antibodies.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 0.5 mL PBS to achieve a final density of 2×105 cells prior to acquisition and were then analyzed by a FACSCalibur flow cytometer (BD Biosciences). The surface marker expression of human MSCs thus enriched was assessed by using flow cytometry following 2 passages ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were later suspended in FACS buffer (98% PBS, 2% FBS) and analyzed by flow cytometry (BD LSR-Fortessa X-20). After the sample data was acquired ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Written informed consent was provided by study participants and/or their legal guardians and 2-5mL of venous blood were collected into CPD vacutainer tubes (BD Vacutainer). Alternatively ...
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bioRxiv - Cancer Biology 2022Quote: ... were subcutaneously inoculated with 1 × 106 LoVo or 2 × 106 LS180 cells in 1:1 mixture of PBS and matrigel (BD Biosciences) into lower right flank ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were washed 2 times in FCM Blocking Buffer then analyzed on a BD Accuri C6 Flow Cytometer (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... 2 µg/ml lysostaphin, 2 mM CaCl2, 10 µl/ml annexin V-APC [BD Biosciences] and 10 µl/ml 7AAD [BD Biosciences]). After 10 minutes incubation in the dark cells were immediately analyzed by flow cytometry using a FACS Aria III (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with PBS in the presence of 2 % BSA and incubated with anti-IFN-γ-PE-CY7 (BD Biosciences), anti-IL-2-APC (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed twice with FACS buffer and strained through a 100 μm filter before sorting CD11b+ microglia and ACSA-2+ astrocytes on a FACS Aria III (BD Biosciences) with a 100 μm nozzle ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed once in a large volume of PBS and post-fixated in 2% PFA in PBS before analysis on an LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing software (version 2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of RBD to hACE-2 on the cell surface was assessed via the mean fluorescent intensity measured by flow cytometry using BD LSRFortessa (BD Biosciences).
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bioRxiv - Neuroscience 2021Quote: ... P0-P1 mouse pups were anesthetized in ice and then eyes were pierced at the edge of corneal by 30G × 1/2 needle (BD, 305106) under stereomicroscope ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with drugs or DMSO 5 days after infection and the fraction of TUBB-expressing cells was measured 3 days after treatment and then every 2 days as the fraction of mCherry-positive cells by flow cytometry on an LSR-II flow cytometer (BD Biosciences). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed 3 times with PBS with Ca2+ and Mg2+ containing 2% FBS and mean fluorescence intensity per cell was measured by LSRII (BD Biosciences) flow cytometer the same day of the assay.