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Citations for Becton, Dickinson and Company :
1901 - 1950 of 2278 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Leukemic cell infiltration was evaluated using immuno-labeling with anti-huCD45 and anti-huCD7 antibodies (see Supplementary Table 2) and a FACS-Canto II flow cytometer (Becton Dickinson, BD). Cells from Femur ...
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bioRxiv - Microbiology 2023Quote: ... yeast isolates were grown overnight for 16 h from a single colony in 2 mL PD broth (BD, Sparks MA) with shaking at 225 rpm ...
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bioRxiv - Microbiology 2024Quote: ... were incubated on ice for 30 min in the dark in 50 ul of PBS containing 2 ul of APC-labeled mouse anti-human CD4 or anti-human CD184 (CXCR4) antibody (BD biosciences 561840 and 560936 ...
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bioRxiv - Immunology 2024Quote: ... We resuspended cells in FACSWash (1x PBS, 2% FBS, 0.2% sodium azide) and acquired events on a LSRFortessa (BD Biosciences), which we analyzed using FlowJo v10 (BD Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... cells were suspended in PBS containing 0.5% FBS and 2 mM EDTA and were sorted with a 100-μm nozzle using BD FACSAria III (BD Biosciences). Sorted cells were pelleted and lysed in RNA lysis buffer (Zymo Research).
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bioRxiv - Immunology 2024Quote: ... in the presence of 100 U/ml of human IL-2 and soluble anti-CD28 antibody (BD Biosciences, Clone: 37.51). T lymphoblasts were cultured at 37 °C in 5% CO2 for 3 days in exponential growth phase ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclei were then stained with 4’,6-diamidino-2-phenylindole (DAPI) and flowed through a fluorescence activated cell sorter (FACS) (BD FACSCanto ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 1-2 mL of staining buffer (depending upon the starting material and yield) and filtered into a FACS (BD) tube ...
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bioRxiv - Plant Biology 2024Quote: ... Protoplasts were stained with 14 µM 4’,6-diamidino-2-phenylindole (DAPI) in PBS and the DAPI negative cells were sorted by BD FACSAria II ...
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bioRxiv - Immunology 2024Quote: ... and memory B cells (CD19+CD27+) were sorted from PBMCs of SLE patients used in scRNAseq experiment 2 using a FACS Aria (BD). 0.4-35k cells per population were lysed in RLT buffer (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: ... Electroporated MEFs were sorted for GFP+BFP+ cells by fluorescence-activated cell sorting (FACS) 2 days later (BD FACS Aria3u), expanded ...
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bioRxiv - Immunology 2024Quote: ... was obtained after 150 minutes of MV with 0.5 mL of cold phosphate-buffered saline (PBS) with 2 mM of EDTA by inserting a standard disposable intravenous catheter (BD Insyte Autoguard ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were lifted by pipetting in FACS buffer (D-PBS, 2% FBS, 0.1% sodium azide) and analyzed on a FACSCelesta (BD Biosciences). At least 20,000 events were collected per sample on the cytometer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 ng/mL ciliary neurotrophic factor (all from R&D Systems, #212-GD, #248-BD, and #257-CF, respectively) supplemented with 1.5 μM retinoic acid ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were surface stained for 30 min on ice in flow cytometry buffer (PBS, 2% FBS, 1mM EDTA, 0.05% sodium azide) supplemented with 10% brilliant stain buffer (BD bioscience) if more than two brilliant violet dyes were used and with anti-CD16/32 (Trustain FcX ...
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bioRxiv - Immunology 2024Quote: ... followed by 30 min in blocking solution (PBS supplemented with FBS 2%, saponin 0.2%, NaAz 0.1%, 1:1000 FC blocker (BD Bioscience; 553142)) ...
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bioRxiv - Immunology 2024Quote: ... To generate fluorescently tagged Env probes 2 μg of biotinylated Env protein was incubated with either 0.5 μg streptavidin-conjugated PE (BD Biosciences) or 0.5 μg streptavidin-conjugated BV786 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... the cells were cultured for 2-3 days and stained with corresponding antibodies to sort knocked-out cells using a FACS Aria flow cytometer (BD).
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bioRxiv - Cell Biology 2024Quote: ... The samples were then washed twice with DPBS containing 2% BSA and 0.01% saponin before flow cytometry analysis using a BD FACSymphony A5 Cell Analyzer (BD Biosciences) at a fixed speed ...
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bioRxiv - Plant Biology 2020Quote: ... and 1 ml of cell suspension was incubated with 2 μl of Nile red solution (0.25 mg ml−1) for 5 minutes before the analysis with a bench flow cytometer (BD Accuri C6, BD Biosciences). To determine Nile red fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... then were seeded into a 96-well plate at a density of 5×103cells/well coated with 10mg/ml Matrigel matrix without growth factor (Cat No: 3432-010-01, BD Biosciences, CA, USA) for 6 hour incubation at 37□ ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Cancer Biology 2020Quote: ... 2000 cells were plated in 8-well chamber slides onto a base layer of growth factor-reduced Matrigel (5 mg/ml, BD Biosciences, Lot A6532) and collagen type I (1.5-2.1 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then blocked in PBS + 5% BSA for 1 hour before addition of primary antibodies (anti-LC3A/B, CST #4108, 1:100; anti-LAMP1, BD #555798, 1:100) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Colonies were grown from frozen stocks by streaking on TSA-II agar plates supplemented with 5% sheep blood (BD BBL, New Jersey, USA). Unless otherwise stated ...
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bioRxiv - Microbiology 2021Quote: ... or LB media (10 g/liter Bacto tryptone, 5 g/liter Bacto yeast extract, 10 g/liter sodium chloride; BD, San Jose, CA) at 30°C ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with PerCP/Cy5.5 or BV421-conjugated anti–mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Off-line samples were taken from the reactors each 5-10 h and the analysis of the GFP expression levels was performed with a BD Accuri C6 (BD Biosciences, CA, USA) based on FL1-A channel (excitation 488 nm ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... the BECs were seeded in 96-well plates coated with collagen type I (5 µg/mL, #354231, BD Bioscience, San Jose, CA, USA) at a density of 104 cells per well ...
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bioRxiv - Immunology 2021Quote: ... were resuspended in defRPMI-1640 with 3% newborn calf serum and Benzonase™ (FACS buffer) in 5 mL FACS tubes and pre-incubated with GolgiStop™ (BD Biosciences) for ∼60 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... both ends of femurs and tibiae were cut with surgical scissors and bone marrow was extracted by flushing 3-5 ml sterile and ice-cold buffer through the bones using a 26G needle (BD Microlance 3 300300). The isolated bone marrow was then filtered through a 40 µM nylon cell strainer (FALCON 352340 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were stimulated with the RBD peptide pool (5 μg.ml−1 final concentration) and brefeldin A (1 μg.ml−1 ; BD Pharmingen, San Diego, CA, USA) at 37 °C overnight ...
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bioRxiv - Immunology 2022Quote: Surface staining: Single cell suspensions were incubated with 5% normal rat serum and rat anti-mouse CD16/CD32 antibody (BD Biosciences close 2.4G2) then stained with fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...
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bioRxiv - Genomics 2024Quote: ... 2.5 x 105 cells were stained for 75 minutes at room temperature with PE Mouse anti-PDX1 Clone-658A5 (BD Biosciences; 1:10) and Alexa Fluor® 647 Mouse anti-NKX6.1 Clone R11-560 (BD Bioscience ...
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bioRxiv - Microbiology 2024Quote: The urine sample obtained from the patient was quantitatively inoculated onto a Columbia agar plate with 5% sheep blood (BD Diagnostics, Heidelberg, Germany) and a MacConkey II-Agar plate (BD Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed three times with PBS for 5 min each and then analyzed by flow cytometry (LSRFortessa, BD Biosciences, MD, USA). The mean fluorescence intensity (MFI ...
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bioRxiv - Biophysics 2024Quote: ... After 5 or 10min of incubation at room temperature (including the 1min centrifugation) cells were fixed with BD fixation buffer (BD Biosciences 51-2090KZ) for 30min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... PerCP-Cy5.5-conjugated anti-IFN-γ (Clone 4S. B3, 1:10), and PE-cy7-conjugated anti-TNF-α (Clone Mab11, 1:50) (BD Biosciences) for intracellular staining ...
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bioRxiv - Physiology 2023Quote: ... p = 0.2 (sum of shared targets between 1/0 and 5/0 R-βHB ex vivo groups and BH-BD targets, divided by n), and q = 0.8 ...
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bioRxiv - Immunology 2023Quote: ... was added to the cell suspension and Fluorescein isothiocyanate (FITC)-positive to FITC-negative cell percentage was measured after 5 min of incubation using the BD FACSCanto™ Flow Cytometer (BD Biosciences, USA). Results were analyzed using the Flowing Software (version 2.5.1 ...
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bioRxiv - Immunology 2023Quote: ... and the cells were stained with antibodies specific for the following proteins: CD4 (PE Rat Anti-Mouse CD4; Clone RM4-5; BD Biosciences; Cat # 553049), H-2Dd (Anti-mouse H-2Dd antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% skim-milk diluted in 1X TBST and incubated with primary antibodies overnight [RB (BD Pharmingen #554136, 1:500), p53 (Pierce #MA5-12453 ...
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bioRxiv - Cell Biology 2024Quote: ... A master mix (5 µL of each fluorochrome-conjugated antibody + 10 µL of Brilliant Staining Buffer (Figure 1 a & b) (BD Horizon™ #659611) or Super Bright Complete Staining Buffer ...
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bioRxiv - Genetics 2024Quote: ... Cells were stained with 5 ml PE Mouse Anti-Cardiac Troponin T antibody (564767, clone 13–11, BD Biosciences, San Jose, CA, USA) for 45 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... jejuni grown on MH agar were resuspended in 5 ml of MH broth in a 14-ml round-bottom tube (BD Falcon, MA, USA) to an optical density at 600 nm (OD600 ...