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Citations for Becton, Dickinson and Company :
1851 - 1900 of 1901 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... The bottom and top parts of the membrane were then incubated overnight at 4°C with primary p53 antibody (2 µg/mL, 1:250) (Clone DO-1, BD Biosciences, Cat. 554293) and primary vinculin antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... the internal space of the chambers in which biofilms were incubated measured 5,000 μm × 500 μm × 70 μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417–11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1 milliliter syringes (Brandzig ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences, 557735], and anti-CD107a [BD Biosciences, 560647]) by 20 minutes of incubation at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: Mouse ESCs were pre-differentiated to an epiblast-like state as previously described [2] by seeding fibronectin-coated dishes (BD Biosciences cat. no. 356008) at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat ...
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bioRxiv - Neuroscience 2020Quote: ... and plated as dissociated cells (25 × 104 cells/well) on day −2 in a 6-well Matrigel-coated plate (BD Biosciences, San Jose, CA). On day −1 ...
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bioRxiv - Plant Biology 2019Quote: ... Chromosomal DNA was then stained by 4’,6-diamidine-2’-phenylindole (DAPI) at final concentration 2 μg/ml and the chromosome suspensions were analysed by FACSAria SORP II flow sorter (BD Biosciences, San Jose, USA) at rates of 1000-2000 particles/sec ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS 2% FBS and analyzed by flow cytometry using a FACSCanto and Novocyte flow cytometer (BD Biosciences and Acea Biosciences). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2020Quote: 2 × 106 cells were stimulated for 4 h at 23°C with 40 ng ml−1 PMA and 1 μg ml−1 ionomycin in the presence of 2 μM Monensin (Golgi-Stop, BD Biosciences, Cat: 51-2092KZ). Cells were washed twice with FACS buffer and stained for extracellular markers ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Immunology 2022Quote: ... the cells were fixed with 2% PFA and stored at 4°C until the samples were acquired on a LSRII cytometer (BD Biosciences, Mississauga, ON, Canada). Data analysis was performed using FlowJo v10.5.3 (Tree Star ...
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bioRxiv - Immunology 2022Quote: Total white blood cells from 1-2 mL freshly collected blood in EDTA tubes were enriched through ammonium chloride lysis (BD Bioscience, catalog no. 555899), followed by centrifugation (300x g for 10min) ...
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Immunology 2019Quote: ... The cells were then washed twice in media and resuspended in 2% paraformaldehyde until they were run on a BD LSRII Flow Cytometer (BD BioSciences, San Jose, CA). Flow data were analyzed using Flowjo version 10.5.3.
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... Cells were collected from wells by pipetting with PBS (containing 2%FBS and 2mM EDTA) and data acquisition was performed on a BD Accuri C6 (Becton, Dickinson and Company, USA). All flow cytometry data were analyzed with FlowJo v10.1 (BD Biosciences).
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bioRxiv - Microbiology 2022Quote: ... After 19h incubation at 37°C, the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville, MD) and fluorescence was evaluated on a LSRII (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2022Quote: ... approximately 1-2 mm apart along the same bundle of muscle fibers with the help of a 20G syringe tip (BD Microlance, Thermo Fisher Scientific). After the dental cement hardened ...
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bioRxiv - Bioengineering 2023Quote: ... Fixed and permeabilized cells were washed twice with PBSA buffer and incubated for 2 hr at room temperature with Alexa Fluor 647-conjugated anti-STAT5 pY694 antibody (BD Biosciences, clone 47/Stat5) diluted 1:50 in PBSA buffer ...
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bioRxiv - Immunology 2019Quote: ... Serum was collected by tail-tipping before LPS injection and 2 and 4 hours following injection for IL6 determination by ELISA (BD; OptEIATM Set Mouse IL-6), as per manufactures instructions.
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended in PBS/2% FBS for analysis on a five-laser flow cytometer (FACSymphony A3 Cell Analyser, BD Bioscience, San Jose, CA, USA). Data were analyzed with FlowJo software (TreeStar ...
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bioRxiv - Neuroscience 2020Quote: Neurons from 18-day-old rat embryos were dissected and dissociated using trypsin and plated at a density of 70,000 cells cm−2 in minimal essential medium (MEM) supplemented with 10% NU serum (BD Biosciences, Le Pont de Claix, France), 0.45% glucose ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at room temperature followed by overnight incubation at 37°C in antibodies to the following: (1) C-terminal binding protein 2 (mouse anti-CtBP2; BD Biosciences, used at 1:200). (2 ...
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bioRxiv - Immunology 2021Quote: ... and intracellular cytokines (PE anti-mouse IFN-γ, PE-Cy®7 anti-mouse TNF-α, APC anti-mouse IL-2, all from BD Biosciences, USA). The labeled cells were acquired with the BD FACSCanto™ II flow cytometer and analyzed with the program FlowJo v.10.6.2 (BD Biosciences).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an air-liquid interface (ALI) in a defined differentiation media [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), and 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we allowed organotypic cultures to differentiate at an ALI in a defined differentiation media supplemented with IL-13 [base media with 2% v/v NuSerum (BD BioSciences, San Jose, CA, USA), 50 nM retinoic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: The actinobacteria were routinely cultured from frozen spore preparations via inoculation into Difco International Streptomyces Project (ISP) medium 2 (ISP2) (Becton, Dickinson and Company, Spark, MD) and incubated at 28° C ...
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bioRxiv - Immunology 2020Quote: ... before being washed and re-suspended in PBS/2% FCS for analysis on a five-laser flow cytometer (LSR FortessaTM; BD Bioscience, San Jose, CA, USA). The data were analysed with FlowJo software (TreeStar ...
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bioRxiv - Immunology 2020Quote: Cells were stimulated for 12h with SARS-CoV-2 peptide pools (0.25 μg/ml) in the presence of αCD28/αCD49d co-stimulatory antibodies (BD FastImmune™, BD Biosciences, San Diego, CA, USA), 5 μg/ml brefeldin A (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (BD Pharmingen 553673 2 μg/ml) at 4 °C for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... PMVECs were purified after two rounds of immune selection using magnetic beads coated with anti-CD31 and anti-ICAM-2 antibodies (BD Bioscience, Franklin Lakes, NJ, USA). mPMVECs were cultured and maintained in a medium containing 5 % FBS and EC growth supplement (ECGS ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then washed 3x in flow cytometry stain buffer and fixed with 2% PFA for 20 minutes prior to acquisition on a LSRII Fortessa (BD Biosciences, San Jose, CA, USA). Samples were analyzed with FlowJo V10 software (Figure S4).
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bioRxiv - Cancer Biology 2023Quote: ... or E0771 hPD-L1 (2 × 105 cells in 25 μL of medium mixed with 25 μL of Matrigel Basement Membrane Matrix [BD Biosciences, San Jose, CA, USA]) were injected into the mammary fat pad or flank ...
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bioRxiv - Genetics 2022Quote: ... The proportion of HbF-and HbS-positive RBCs were measured with an antibody recognizing HbF (Thermo Fisher for HUDEP-2 cells or BD Biosciences for primary cells) or HbS (BioMedomics) ...
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bioRxiv - Neuroscience 2023Quote: ... Microdissected pieces were immunostained with antibodies to the following: (1) mouse anti-C-terminal binding protein 2 (pre-synaptic-marker, BD Biosciences, 1:200, Cat # 612044), (2 ...
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bioRxiv - Cancer Biology 2023Quote: ... BD 560046. After washing twice, cells were fixed in PFA 2% for 20 min, washed, and acquired on a Fortessa BD LSRII Cell Analyzer (Becton Dickinson).
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bioRxiv - Synthetic Biology 2019Quote: ... with 2% (w/w) PEG-PFPE amphiphilic block copolymer surfactant (Ran Biotechnologies, 008-FluoroSurfactant) were loaded into separate syringes (BD, 1 mL and 5 mL, respectively) and injected with 23 gauge needles through PTFE tubing (0.022” ID ...
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bioRxiv - Cancer Biology 2021Quote: ... Clones were picked after 2–3 months of culture and expanded in the same medium on dishes coated with Collagen I (BD Biosciences, Le Pont de Claix, France) in humidified atmosphere with 5% CO2 at 37°C.
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bioRxiv - Physiology 2021Quote: ... cells were washed twice with FACS buffer (PBS plus 2% heat inactivated fetal bovine serum) and analyzed on a SORP 8 Laser BD LSR flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ).
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Biophysics 2024Quote: ... transferred in a V-bottom 96-well plates and co-cultured with K562 cells (ATCC) at 1:1 effector-to-target ratio in the presence of mouse anti-human CD107a Alexa Fluor 647 (BD Bioscience, clone H4A3, 2 uL/well) for 6 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).