Labshake search
Citations for Becton, Dickinson and Company :
1801 - 1850 of 1901 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Samples were stored at 10-15°C in 2 mL K2-EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 15 to 45 min later.
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bioRxiv - Physiology 2022Quote: ... The sample was stored at 10-12 °C in 2 mL EDTA (ethylenediaminetetraacetic acid) tubes (BD Vacutainer®, Franklin Lakes, NJ, USA) without agitation until analysed 0.5-2.5 h later ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were blocked with 2% BSA in 0.1% tween TBS (TBST) for 1h and immunostained with mouse anti-paxillin IgG1 antibody (BD Transduction Laboratories 610052) (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Cell Biology 2023Quote: ... around 2×105 HEK001 cells expressed GFP (GFP+ HEK001) isolated by FACS Aria II cell sorter (BD Biosciences, San Jose, CA, USA) and cultured in one well of a 24-well plate ...
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bioRxiv - Microbiology 2023Quote: ... Blood was collected via cardiac puncture using a 1mL 28G1/2 insulin syringe and added to a serum collection tube (BD Microtainer 365967). Tubes were incubated for 45 minutes at room temperature to facilitate blood coagulation and then spun at 4 °C for 10 minutes (1500 x g) ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ produced upon re-activation of peptide-specific T cells and captured on anti-IFNγ capture antibody was visualized via sequential addition of 2 μg/mL biotinylated anti-IFNy detection antibody (BD #51-1818KA), Streptavidin-Horseradish Peroxidase (HRP ...
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bioRxiv - Immunology 2023Quote: ... and plated in round-bottom 96-well plates at 1-2 x 106 cells per well in complete RPMI (cRPMI) medium containing CD28 costimulatory antibody (BD Biosciences #553294) and brefeldin A ...
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bioRxiv - Biochemistry 2023Quote: ... Cells with the highest 2-5% GFP signal were selected two to three days post transfection using a flow cytometer (BD FACSAria II). Single cells were cultured in RPMI with 10% FBS ...
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bioRxiv - Systems Biology 2023Quote: ... all strains are grown in YPD (2% w/v Bacto Peptone, BD Biosciences #211677; 2% w/v D-glucose, BD Biosciences #215520; 1% w/v Yeast Extract, BD Biosciences #212750) at 30°C.
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bioRxiv - Synthetic Biology 2023Quote: ... 2% dextrose) when containing no plasmids and in synthetic defined (SD) media (0.67% yeast nitrogen base without amino acids [BD Difco], 2% dextrose) supplemented with the appropriate yeast synthetic drop-out medium supplement when plasmid maintenance was required (SDΔW-without tryptophan ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... They were stained for 2 hours at room temp with the following primary antibodies: mouse anti-GM130 (1:250, BD Bioscience, BDB610822) and rabbit anti-TGN46 (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... for 20min at 4°C and stained with intracellular antibodies including anti-mouse IFN-γ-Pe-Cy7 and IL-2-BV605 (BD Bioscience) for 30min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained for 30min on ice with Alexa-Fluor-488-conjugated mouse monoclonal antibody (mAb) directed against the SARS-CoV-2 spike protein (R&D systems; clone #1035206, diluted 1:50 in Perm/Wash buffer (BD)) ...
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bioRxiv - Immunology 2024Quote: ... MASP-1/-3/MAP-1 mab 8B3 or F(ab’)2 fragments generated from an irrelevant mouse IgG1 mAb clone MOPC-31C (BD Biosciences, 557273) were coated on maxisorp plates at 4 °C ON and afterwards washed and blocked for 1 hr in TBS/T ...
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bioRxiv - Immunology 2024Quote: ... and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD, Cat#566385) for 30 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Positively transduced cells were selected with 2 µg/ml puromycin followed by a sterile sort for high GFP and mCherry expression (BD FACSAria II). Expression was maintained with 1 µg/ml puromycin in all experiments except where immune cells were needed.
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bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
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bioRxiv - Cancer Biology 2024Quote: CAR T-cells were incubated with target cells at an effector-to-target (E:T) ratio of 2:1 in complete RPMI 1640 medium containing GolgiStop™ Protein Transport Inhibitor Reagent (BD Bioscience, USA) and CD107a APC antibody (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... cell suspensions were resuspended in PBS containing 2% FBS and then separated on a FACSAria Fusion flow cytometer (BD Biosciences, San Jose, CA). For single cell sorting ...
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bioRxiv - Cancer Biology 2019Quote: ... of both preparations was determined by titration onto the target cell line and quantification of the percentage of BFP positive cells 2-3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Cell Biology 2019Quote: ... for 2 hours at room temperature followed by overnight incubation at 4°C with anti-PECAM-1 (MEC13.3, BD Bioscience #550274, 1:250) diluted in blocking buffer ...
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bioRxiv - Genomics 2021Quote: ... anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences, Custom Bulk 624060, and Pacific Blue; BD Biosciences, Custom Bulk 624034), anti-CD4 (L200 ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 2 - 4×105 events were collected per sample within 3 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Microbiology 2019Quote: Spiral plating was conducted using an Autoplate 400 Eddy Jet 2 spiral plater (IUL, Barcelona, Spain) onto Lactobacilli deMan Rogosa and Sharpe (MRS) agar (Becton, Dickinson and Company) supplemented with 1% cycloheximide (Remel ...
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bioRxiv - Systems Biology 2022Quote: ... coli strain JM109 (1 × 1010 colony-forming units (CFU)/ml) were cultured for various times in 2 ml of 2xYT liquid medium (BD Difco, Cat# 244020) containing various concentrations of pyridoxal 5’ -phosphate (pH adjusted to 7.0) ...
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bioRxiv - Microbiology 2020Quote: ... Cultures with vesicles were incubated for 2 hours at the normal growth temperatures and examined on an Influx flow cytometer (Cytopeia/BD, Franklin Lakes, NJ). Cells were excited using a blue 488 nm laser and monitored for chlorophyll (692/40 nm emission ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation for 2 hours at room temperature with primary antibody (SOX2 [1:200, EMD Millipore AB5603], NESTIN [1:200, BD Pharmingen #556309]). After washing five times with PBS/0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: The HLA-A2 status was confirmed by PBMCs staining with 2 μL of anti-HLA-A2 mAb (clone BB7.2) (BD Pharmingen, Franklin Lakes, NJ), at 4°C for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Between 2 - 4×105 events were collected per sample within 24 hours after staining on a LSRFortessa (BD Biosciences, 4 lasers, 18 parameters) and analyzed using FlowJo software (version 9.7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were immediately split into 3 fractions: 2 ml were transferred to heparin and EDTA tubes respectively (both BD, Franklin Lakes, New Jersey). Heparin samples were then centrifuged (1000x g ...
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bioRxiv - Cancer Biology 2019Quote: ... A pure polyclonal population of dCas9-KRAB expressing cells was generated by 2 rounds of fluorescence activated cell sorting (FACS) gated on the top half of BFP positive cells (BD FACS Aria II). The performance of A375_i in knocking down endogenous genes was evaluated by individually targeting 3 control genes (ST3GAL4 ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... approximately 200 μL cells were added into the flow tube containing 2 mL PBS and tested by the flow cytometry (BD, New Jersey, USA). Three independent assays were performed.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed with Hank’s buffered saline solution and resuspended in 2mM EDTA/ 2% BSA/ PBS buffer containing [2µg/mL] propidium iodide (PI) (BD Biosciences, Cat. No. 556364) and [1µM] Calcein violet (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Female C57BL/6J mice aged 8 – 12 weeks were injected intraperitoneally with 2 ml each of 3% sterile thioglycollate (BD Biosciences, catalog # 211716) medium ...
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bioRxiv - Cell Biology 2022Quote: ... 10 min at room temperature) and the supernatants were collected to measure the levels of IL-2 by ELISA using the OptEIA™ kit (BD Biosciences, USA), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2.
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bioRxiv - Immunology 2022Quote: ... cells counted under the microscope and stained in FACS buffer (PBS, 2% FCS, 2mM EDTA) with purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, BD Catalog #553141) and the specific antibody cocktail ...
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bioRxiv - Immunology 2022Quote: ... Spleens were disrupted using a syringe in 2% FBS/PBS and passed through a 40 μm nylon cell strainer (BD, catalog no. 352340) to obtain a single-cell suspension ...
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bioRxiv - Pathology 2023Quote: ... Cells were diluted in basal medium containing 2% dextran-coated charcoal-stripped serum (DCC) (HyClone Laboratories, Logan, UT) and plated into culture dishes (BD Falcon, Bedford, MA). Cells were cultured for 24 hours in an incubator at 37°C with saturating humidity and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... All 5 samples were then pooled and stained for surface markers in 70μl of the antibody cocktail (Supplementary Table 2) in BD Horizon Brilliant Stain Buffer Plus (BD Biosciences, San Jose, CA) for 30 minutes at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...