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Citations for Becton, Dickinson and Company :
1851 - 1900 of 8463 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Cell Biology 2022Quote: ... filtered with a 3 μm filter and sorted for YFP expression into a 96 well plate (5 and 10 events; 48 wells each) (FACSARIA III, BD Biosciences). Individual plaques were screened by PCR and all modified loci were sequenced to confirm correct modification ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were transferred to a 50 ml tube and 9 ml LB (10 g/l Peptone (OXOID, LP0037), 10 g/l NaCl (ACROS, 207790010) and 5 g/l Yeast Extract (BD, 211929)) were added with 50 μg/l kanamycin (ACROS ...
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bioRxiv - Immunology 2022Quote: ... overlapping peptides (10µg/mL) or media alone for 60 hours at 37 °C and 5% CO2 with proliferation dye (Violet Proliferation Dye, BD Biosciences). Peptides were generated in 15mer format with 8 amino acid overlaps for the ID91 fusion antigen ...
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bioRxiv - Genomics 2022Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The cells were then blocked with 5% BSA for 20 minutes before being stained with antibodies against CD19-PE (BD-555413), CD3-BV510 (BD-564713) ...
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bioRxiv - Immunology 2022Quote: ... cells were washed 2x with FACs buffer at 1000xg for 5 minutes at room temperature and analyzed on a LSR II (BD Biosciences) at the Flow Cytometry Shared Resource at the University of Arizona ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 5-6 days and CFSE dilution of T cells was determined on a BD Canto II (BD Bioscience) and analyzed by FlowJo (v10.1 ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were analyzed for proliferation at day 5 via Cell trace dye (eBioscience) and inhibitory receptor expression (LAG3 and PD1) on a Fortessa II (BD Bioscience) and data analyses were performed via FlowJo (Tree Star).
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells (5 × 104 cells per well) were plated in 24-well culture plate and the top chamber (24-well insert; BD Bioscience) were seeded with MCF7 (2 × 104 cells ...
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bioRxiv - Biochemistry 2020Quote: ... to stain for CD69 receptor using 5 µL (100 µg/mL) of PreCP-Cy ™ 5.5 mouse anti-human CD69 (BD Biosciences). Staining was done for 45 mins on ice then washed one time with 2 mL RPMI-1640 medium and reconstituted in 250 µL RPMI-1640 medium ...
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bioRxiv - Bioengineering 2021Quote: Cells were plated at 5-15K the day prior to transfection in a 96-well plate coated with poly-D-lysine (BD Biocoat). HEK293FT and HepG2 cells were transfected with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Spleen cells of immunized mice were incubated with 50 μM of each peptide for 5 hours at 37°C in the presence of brefeldin A (GolgiPlug, BD Biosciences), and were stained with FITC-conjugated anti-mouse CD8 mAb (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Non-adherent cells transduced with PMG based vectors were typically sorted for top 5-20% ZS-green expressing cells once on a FACSAria III (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: HeLa cells were trypsinized and resuspended in serum-free media supplemented with 5 mM EDTA prior to measurement on an Accuri C6 plus cytometer equipped with 488 and 640 nm lasers (BD Biosciences). Untransfected cells were gated out during analysis.
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bioRxiv - Immunology 2019Quote: ... 7H9 containing 0.1% SDS in the required plate and incubating for 5 min and plating on 7H11 agar plates supplemented with OADC (BD Difco). The plates were incubated at 37°C to allow bacterial growth ...
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bioRxiv - Microbiology 2020Quote: ... pigrum strains were cultivated from frozen stocks on BBL Columbia Colistin-Nalidixic Acid (CNA) agar with 5% sheep blood (BD Diagnostics) for 2 days ...
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bioRxiv - Immunology 2021Quote: ... lamina propria cells were stimulated for 5 h with PMA (50 ng/ml) and ionomycin (500 ng/ml) in the presence of BD Golgi plug (BD Biosciences) followed by fixation and permeabilized using the BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Immunology 2019Quote: ... for 5 min at room temperature before acquisition and sorting of the cells using a BD FACSARIA III (BD BioSciences, USA). Single antibody staining was prepared in parallel to assess fluorescence spillover ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 5 μg/mL puromycin and RFP+ cells were collected using fluorescence-activated cell sorting (FACS) on a FACSAria (BD Biosciences). RFP+ cells were serially diluted in a 96-well plate to generate single cell clones ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were plated at a density of 1.5×106 cells/well on 18mm coverslips (Neuvitro 18-GG-PDL) precoated with high concentration growth factor-reduced Matrigel (BDBiosciences, 354263) and cultured at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... A minimum of 5 000 – 10 000 cells was analyzed per sample for YFP expression on a LSRII flow cytometer (BD Biosciences). Data was analyzed using Flowing Software (Version 2.5).
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bioRxiv - Immunology 2022Quote: ... and tissue-resident alveolar macrophages (TRAMs) were flow-sorted using a BD FACSaria III 5-laser cell sorter and 100 μm nozzle (BD Biosciences). Of note ...
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bioRxiv - Immunology 2020Quote: ... and 2 mM KH2PO4)-FBS 5% for 15 min and then stained for surface expression with fluorophore-marked antibodies against SLAMF1 (BD, 559572), CD31 (BD ...
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bioRxiv - Microbiology 2020Quote: ... The resulting bacterial suspension was serially diluted and plated on BD Group A Selective Strep Agar with 5% Sheep Blood (BD Diagnostics) or THY agar plates with appropriate antibiotic selection ...
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... CD8+EGFP-Tconv and CD8+EGFP+ Treg cells in blood and spleen as well as the expression levels of Stat-5 phosphorylation (clone pY694, BD Biosciences) and CD25 (clone OX39 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
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bioRxiv - Immunology 2021Quote: ... All samples were acquired with a FACS Canto II or 5 laser Fortessa with BD FACS Diva software and analysed with FlowJo software (versions 9 and 10; BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Genomics 2022Quote: ... and then dounce homogenized (10 loose strokes and 5 tight strokes) and filtered through 35-40μm cell strainers (BD Biosciences, 352235). Nuclei were pelleted (600g ...
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bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
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bioRxiv - Bioengineering 2022Quote: ... The specificity of CD8+ T cells towards the peptide was previously assessed by co-culture of 5·104 T cells with pulsed tumor cell lines in the presence of Golgi Plug (BD Biosciences) in 96-well plate at 1:1 E:T cell ratio.
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bioRxiv - Microbiology 2023Quote: Cells from BALF were centrifuged at 300 x g for 5 minutes and incubated in 500μl Pharm Lyse Buffer (BD Biosciences 555899) for 3 minutes at room temperature to lyse red blood cells ...
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bioRxiv - Cancer Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and non-neuronal (NeuN-) nuclei were sorted into tubes pre-coated with 5%BSA using a BD-FACSAria flow cytometer (BD Biosciences) equipped with a 70μm nozzle (Figure S6) ...
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bioRxiv - Immunology 2023Quote: ... P14 T cells (Figure 5) were FACS-sorted from the spleen or LNs of chronically infected mice using a FACSAria III cell sorter (BD Biosciences). To multiplex different samples for scRNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Phycoerythrin (PE) or peridinin chlorophyll protein -cyanine 5.5 (PerCP-Cy5.5)-conjugated CD3 (BD Biosciences Cat#5 61808, BD Biosciences Cat# 55163); Pacific Blue-conjugated (PB) ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were washed twice with HBSS-5%FBS and once with PBS and analysed by flow cytometry (BD FACScalibur, BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... The cells were cultured at 37°C and 5% CO2 for 72 hours and then the dye dilution was analyzed using a FACSVerse flow cytometer (BD Biosciences), and data were analyzed using FlowJo software (Tree Star).
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bioRxiv - Bioengineering 2023Quote: ... and then cells were re-suspended in 150 μL of PBS with 5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression.
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bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
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bioRxiv - Immunology 2023Quote: ... Cells were acquired on a Cytek Aurora spectral flow cytometer using Cytek SpectroFlo software (Cytek; the HD BM samples) or a LSR Fortessa X20 (special order research product with 5 lasers; BD Biosciences) and analyzed using FlowJo software (v10.8.1 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
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bioRxiv - Systems Biology 2023Quote: ... A PDMS chamber was put on the mold and a solution of collagen rat tail I (5 mg/ml, BD Biosciences) was injected and allowed to polymerize at 37 °C for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... while 100 μg was subjected to chromatin immunoprecipitation (ChIP) using 5 μg of mouse anti-ASCL1 (Mash1) antibody (BD Biosciences, 556604) or 5 μg rabbit ant-OLIG2 (Olig2 ...