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Citations for Becton, Dickinson and Company :
1751 - 1800 of 8463 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were washed with 1XPBS-4%FBS before incubation for 20 min on ice at 5•107 cells/ml with 500 ng/ml Fc block (2.4G2, BD Pharmingen). Cells were then incubated for 1 hour on ice with AF700 conjugated CD45.2 monoclonal antibody (104 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted for 5 min at 650 g and resuspended in PBS to be immediately analyzed using a Becton Dickinson (BD) LSRFortessa ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Cell Biology 2023Quote: ... and stained in 100 µL of FACS buffer (HBSS with 0.1% BSA w/v, 5 mM EDTA) containing mouse antibodies anti-CD45 (BD Sciences), anti-CD11b (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... Experimental starters were prepared from frozen master stocks plated on 5% Columbia blood agar plates (Biomerieux ref no.43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50mM HEPES (TH+H ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then washed using FACS Wash Buffer containing 1x D-PBS + 0.5% FBS + 2mM EDTA and permeabilized at room temperature for 20 minutes using 100µL of Perm/Wash I (BD # 557885), diluted 1:10 with 1x D-PBS ...
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bioRxiv - Cell Biology 2023Quote: ... AML cells were incubated at 37°C (in 5% CO2 and air) in 96-well round-bottom plates (BD Biosciences) at 5 to 10 × 103 cells per well containing various concentrations of the Bispecific T cell engager (generated from published sequences using methods similar to those reported previously60 ...
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bioRxiv - Genetics 2023Quote: ... and the pellet was resuspended in 300 µL PBS with 5% FBS for flow cytometry using a CantoII (speed high; BD).
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bioRxiv - Immunology 2023Quote: ... were co-cultivated with 5×105 IE1-CTLL cells for 5h at 37°C in the presence of brefeldin A (BD GolgiPlug ...
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bioRxiv - Immunology 2023Quote: ... Sorting was conducted at room temperature and sorted cells were collected in 5 ml round-bottom polystyrene tubes (BD Biosciences) containing 500 μl of complete medium (RPMI 1640 supplemented with 10% FBS and 1% penicillin/streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of the stain cocktail (1x Brillant Stain Buffer [BD Bioscience] and either 5 μL per sample of Alexa Flour 647 Anti-Total Stat1 [N-Terminus] [BD Biosciences] and 5 μL per sample of PE anti-STAT1 Phospho [Tyr701] [BioLegend] ...
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bioRxiv - Microbiology 2023Quote: ... Experimental starters were made from master glycerol stocks struck on 5% Columbia blood agar plates (Biomerieux Ref# 43041) and grown overnight at 37°C with 5% CO2 prior to outgrowth in Todd-Hewitt (BD) broth supplemented with 50 mM HEPES (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Pools of ciliate cells (5 – 50 cells) were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter ...
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bioRxiv - Immunology 2023Quote: ... detection antibody (IFNγ, BD Biosciences #51-1818KA; IL-17A, Invitrogen #88-7371-88; and IL-5, BD Biosciences #51-1806KZ) was diluted in ELISpot diluent (eBiosciences ...
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bioRxiv - Bioengineering 2023Quote: ... A tissue suspension was constituted by adding 5 mL of Tumor & Tissue Dissociation Regent (TTDR, BD Bioscience, San Jose, CA), and then incubating the mixture at 37 °C for 30 min with frequent agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes containing the transferred proteins were rinsed and blocked in 0.1% Tween-20 in Tris-buffered saline (TBST) containing 5% nonfat milk (BD Biosciences) at RT for 1 h ...
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bioRxiv - Genomics 2023Quote: The following antibodies were used for Immunoblot analysis (Extended Data Table 5): Purified Mouse Anti-PTEN (BD Bioscience, Cat# 559600), Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2023Quote: ... high-binding 96-well plates were coated with 5 μg/ml anti-CD3 (Clone UCHT1, BD Pharmingen™ #555329, USA) in PBS (ThermoFisher Gibco™ #21600044 ...
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bioRxiv - Physiology 2024Quote: ... Cells were then pelleted at 500 x g for 3 min at 4°C and resuspended in 25 μL of 5 mg/mL FcBlock (rat anti-mouse CD16/32, clone 2.4G2, BD Biosciences) diluted in FACS Buffer and incubated on ice for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 % donkey serum in PBST containing 0.2% Triton X-100) and incubated with α-EPS8 antibody produced in mouse (BD Transduction Laboratories™ ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice (900 x g for 5 minutes at room temperature) and then 50µL of surface antibody cocktail diluted in Brilliant Violet buffer (BD Biosciences) added and incubated 20 minutes at room temperature in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... collected by centrifugation at 300 g for 5 min and passed through a 35-µm cell strainer filter (BD Biosciences). To assess viability ...
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bioRxiv - Microbiology 2024Quote: Sequential subcultures of all phenotypic variants were carried out on various agar plates (including Columbia agar + 5% sheep blood, MacConkey agar from BD, and CHROMID® CPS® Elite agar from bioMérieux ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells (1.5 x 106 cells) first underwent Fc receptor blockade with rat anti-mouse FcψRIII/II receptor (CD16/32; BD Biosciences). After blocking for 5 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... 5×104 alive GAFs or glioma cells were incubated with PE Mouse anti-Human CD105 (2µg/mL, BD Biosciences #560839) and APC Mouse anti-Human CD90 (2µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Tregs were then sorted on a BD FACS Aria Fusion based on viability dyenegative CD4+ (BV605, clone RM4-5, BD) GFP-FOXP3+ ...
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bioRxiv - Immunology 2024Quote: ... live EGFP+ or EGFPneg Tcf7+/EGFP P14 CD90.1+ TE (Zombie Aquaneg EGFP+ or EGFPneg CD8a+ CD90.1+) from helped or helpless spleens were sorted on a 5-laser FACS Aria II sorter (BD Biosciences). Small aliquots of sorted samples were checked for purity (on average >98%) ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 5 hours with a protein transport inhibitor containing brefeldin A (BD Biosciences, Franklin Lakes, NJ, USA), washed and fixed for 10 minutes at 4°C with fixation/permeabilization solution (BD Cytofix/Cytoperm kit ...
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bioRxiv - Immunology 2024Quote: ... Mouse peripheral blood was collected via submandibular bleed using Goldenrod 5 mm animal lancets (MEDIPoint) into K2EDTA microtainer tubes (BD). For all specimen types ...
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bioRxiv - Microbiology 2024Quote: ... A single isolated colony was then inoculated in 5 ml of BHI broth (BD, cat. no. 237500, Franklin Lakes, NJ) in a 15 ml conical tube (VWR ...
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bioRxiv - Neuroscience 2020Quote: ... one section out of ten was incubated with BrdU antibodies (BrdU, 1/1,000, CldU, 1/500, Accurate Chemical; IdU, 1/500, BD Bioscience). Bound antibodies were visualized with Cy3-goat anti-rat antibodies (1/1,000 ...
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bioRxiv - Immunology 2020Quote: ... RORγt (AFKJS-9; PE, dil. 1:100) (eBioscience); CD25 (PC61; PerCP, dil. 1:400), CD103 (M290; PE, dil. 1:150) (BD Pharmingen); CD11b (M1/70 ...
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bioRxiv - Immunology 2019Quote: ... after which cells were stained with Fixable Viability Staining e780 (1:1000) and CD11B (M1/70) antibodies: CD11B-BV510 (Day 1 cells, 1:300, BD Biosciences), CD11B-Percp-Cy5.5 (Day 3 cells ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 parasites were collected and incubated with VSG2WT antisera (1:4000) or VSG11WT (1:1000) together with Fc block (1:200, BD Pharmingen) in cold HMI-9 without FBS for 10 min at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The cOBBs were resuspended in 1:1 v/v ECM gel to cOBBs and transferred to a 1 mL disposable syringe (BD Biosciences). The cOBBs were centrifuged at 100g for 3 min and the supernatant removed ...
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bioRxiv - Cancer Biology 2024Quote: ... bilateral subcutaneous dorsal flank tumors were established by injecting 1 × 106 cells suspended in a 1:1 mixture of serum-free medium and Matrigel (BD Biosciences). Once tumors reached an average volume of 100 mm3 ...
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bioRxiv - Bioengineering 2024Quote: ... Viability dye was quenched by washing with FACS buffer (PBS + 2% FBS + 1 mM EDTA) before addition of surface staining antibodies in a 1:1 dilution of Brilliant Stain Buffer (BD 563794) in FACS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... and stained for 30 min on ice with surface antibodies at a 1:200 dilution in a 1:1 mixture of FACS buffer and Brilliant Stain Buffer (BD Biosciences). Cells were washed with FACS buffer and PBS and fixed with 2% paraformaldehyde on ice for 20 min ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... then fixed in 2% paraformaldehyde and stored at 4°C until acquisition on a FACSAria Fusion III flow cytometer (BD). Flow cytometry data was analysed with FlowJo version 9.9.6 (Tree Star).
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/mL of anti-mouse IgE (553413, clone R35-72, BD Pharmingen) in 0.1 M sodium carbonate buffer pH 9.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... with 4′,6-diamidino-2-phenylindole (DAPI) as a viability dye and the following antibodies: anti-CD31 PE (BD Biosciences), anti-CD45 APC (Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: Yeast strains were grown overnight in uracil-free minimal medium containing 3% glucose and 1% casamino acids (Cat #223050, Becton, Dickinson and Company, Franklin Lakes, NJ) and offered 10 μCi/ml [3H]myo-inositol (ART 0116A ...
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bioRxiv - Microbiology 2022Quote: ... oxysporum conidia were isolated after growth for 3-4 d in potato dextrose broth (PDB; Becton, Dickinson and Company, Le Pont de Claix, France) by filtering through two layers of Miracloth® (Merck Millipore ...
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bioRxiv - Synthetic Biology 2022Quote: ... all samples were mixed 1:1 with prewarmed CytoFix Fixation Buffer (BDBiosciences #554655) and incubated at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-MCM2 (Cell Signaling, 4007S; 1:1000; BD Biosciences, 610701; 1:1000), rabbit anti-MCM3 (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Pdcd6ip (AIP-1/Alix) (BD transduction lab 611620, 1:500); goat polyclonal anti-TSG101 (Santa Cruz sc-6037 ...
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bioRxiv - Microbiology 2020Quote: ... 250 µg mL−1 L-cycloserine and 1.5% weight vol−1 (BD, USA) (TCCFA plates) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-nestin (1:200 in vivo, 1:500 in vitro, BD Bioscience), chicken anti-vimentin (1:10,000 ...