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Citations for Becton, Dickinson and Company :
1801 - 1850 of 8232 citations for Goat Anti Human IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Multiscreen ninety-six well plates were coated overnight with 100 μl per well of 5 μg/ml anti-human interferon-γ (IFN-γ) (B27; Becton, Dickinson and Company, Franklin Lakes, NJ) in endotoxin-free Dulbecco’s-PBS (D-PBS) ...
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bioRxiv - Microbiology 2020Quote: Human lymphocyte characterization was performed on an LSR II (BD Biosciences). Lungs from BLT mice were minced using scissors and incubated in medium containing Liberase (0.02 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Human recombinant growth factors PDGF and EGF were purchased from BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Human CD19-APC and CD69-APC antibodies were obtained from BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Intracellular staining was carried out using Human FoxP3 Buffer (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Human umbilical vein endothelial cells (HUVEC; BD Bio-science, cat # 354151) were seeded at passage 4 in a T75 flask with a density of 10.000 cells/cm2 ...
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bioRxiv - Immunology 2021Quote: ... using a human IL-1β enhanced-sensitivity flex set (BD Biosciences). Undiluted cell supernatant was stained according to the manufacturer’s instructions and run on a BD FACS Canto II ...
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bioRxiv - Cell Biology 2021Quote: ... Human PSC lines were maintained on 1:120 Matrigel™ (BD) in phosphate-buffered saline (Thermo Fisher Scientific)–coated plates and cultured in StemMACS iPS-Brew XF (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Human IL-1β enzyme linked immunosorbent assay (ELISA) kit (BD, #557953) and human IL-18 ELISA kit (R&D ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic stem cells were grown on ESC-qualified Matrigel (BD) in human ES media consisting of KO-DMEM (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Human IL-1β enzyme linked immunosorbent assay (ELISA) kit (BD, #557953) was used according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Human KCNQ2 cDNA (GenBank accession number NM_172108) in pIRES2-EGFP (BD Biosciences-Clontech ...
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bioRxiv - Molecular Biology 2024Quote: Human blood fresh citrate-anticoagulated blood (105 mM Na3-citrate, BD-Vacutainer® ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in buffer solution (PBS + 2% FBS + 2mM EDTA) and blocked with recombinant Fc protein (1:100 dilution, BD Biosciences, cat#553142). Cells were then incubated with the primary antibody solution (1:100 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with 1x Permeabilization buffer (eBioscience) and incubated for 15 min in FACS buffer (PBS, 2% FCS) before adding the primary MASH-1 antibody (BD Biosciences, 556604), 1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with Fc block (eBiosciences 16-0161-81) for 5 min then incubated with antibodies for CD45-APC (BD Biosciences 561018) and CD11b-BV421 (BD Biosciences 562605 ...
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bioRxiv - Cancer Biology 2021Quote: ... Reactions were terminated by addition of DMEM containing 8% FCS and cell suspensions were dispersed through a 70 μm cell strainer (BD Falcon, 352350). All single-cell suspensions were treated with RBC lysis buffer to remove red blood cells ...
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bioRxiv - Genetics 2022Quote: ... and ICAM1-Fc (a gift from Professor Alister Craig, Liverpool School of Tropical Medicine) spotted on bacteriological petri dishes (BD Falcon 351007) at a concentration of 50μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed twice with FC buffer by centrifugation (1000 rpm/5minutes) and incubated with streptavidin−PE conjugate (BD bioscience, 349023) for 30 minutes at 4ºC ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in 100 μL of PBS containing 5% FBS and blocked with 2.5 μg of Fc-blocking solution (BD Bioscience, Cat #564220) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent was washed twice, incubated with DR (Bio-Legend, 424101, 1:1000) and CD16/CD32 Fc block (BD Biosciences, 553141, 1:200) at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD, Franklin Lakes, NJ, USA, cat.: 562440), anti-human CD20 (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: Human microglia cell line was plated in T150 flasks (BD Falcon 355001) at 20 million/flask in 25mL complete microglia media and placed in 37C 5% CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with α-human CD8-FITC (G42-8, BD Pharmingen), CD69-BV605 (FN50 ...
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bioRxiv - Bioengineering 2020Quote: ... and ITS+ Premix (1%) and human EGF (20 ng/ml) from BD Biosciences.
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bioRxiv - Systems Biology 2021Quote: Gardnerella vaginalis isolate 11E4 were maintained on Human Bilayer Tween Agar (BD) plates and NYC III medium according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The human inflammatory cytometric bead array (CBA) kit was purchased from BD Biosciences (San Jose ...
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bioRxiv - Microbiology 2020Quote: ... gondii tachyzoites (RH strain) were maintained in hTERT human fibroblasts (BD Biosciences) using Dulbeco’s modified essential media (DMEM ...
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bioRxiv - Immunology 2020Quote: ... ELISA kits for detection of mouse IL-1β was from BioLegend and for mouse IL-12p70 and human IL-1β were from BD. Pertussis toxin ...
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bioRxiv - Immunology 2022Quote: ... The reagents used include Human IL-1β ELISA kit (BD Biosciences, 557953), anti-FLAG antibody (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... human PBMCs (3×105 cells/condition) were incubated with CD8 (BD, 562428), CD25 (BD ...
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bioRxiv - Neuroscience 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained on Matrigel (BD Biosciences) in complete mTeSR Plus (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2023Quote: Human blood was collected into a 3.2% sodium citrate tube (BD Vacutainer) and centrifugated (1,200 x g for 10 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... TNF-α and interferon (IFN)-α were quantitatively determined by BD™ CBA Human Th1/Th2 Cytokine Kit II (BD Biosciences, San Jose, CA) as described previously[18] ...
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bioRxiv - Bioengineering 2024Quote: ... and ITS + Premix (1%) and human EGF (20 ng/mL) from BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and ITS + Premix (1%) and human EGF (20 ng/mL) from BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with antibodies against human CD45 (clone HI30, BD Biosciences) and mouse CD45 (clone 2D1 ...
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bioRxiv - Immunology 2020Quote: ... The cells were then washed and resuspended in PBS supplemented with 2% FCS for FACS analysis (FACSCanto II; BD Bioscience, San Jose, USA). FACS data analysis was performed using BD FACSDivaTM Software 8 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: We permeabilized formalin-fixed glomerular and tubulointerstitial single cells with 0.25% Tween-20 in PEB buffer at room temperature for 30 minutes and blocked cells with Fc Block (clone 2.4G2, BD Biosciences, San Jose, CA) diluted in 0.25% Tween-20 on ice for 10 minutes ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Cells were incubated in the dark with 2 μl/1×106 cells of CD16/32 antibody (Fc block; BD Biosciences, Mississauga, ON, Canada) for 15 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... samples were stained with the relevant combination of surface and intracellular antibodies in combination with Fc blocking antibody CD16/CD32 (BD Pharmingen, Cat. #553141). BV421 anti-MHCII(IA/IE ...
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bioRxiv - Immunology 2022Quote: ... blood cells were stained in 50 µl 2% FCS RPMI medium with antibodies against CD11C (APC, clone HC3, BD biosciences, dilution 1/100), CD11b (PercPCy5.5 or Pacific Blue ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... cells were restimulated ex vivo for 4-5h at 37 °C in complete RPMI medium (RPMI supplemented with 2mM L-Glutamine and 10% fetal calf serum (FCS)) with GolgiPlug (BD, 1:1,000 dilution), phorbol 12-myristate 13-acetate (50ng/ml ...
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bioRxiv - Genomics 2021Quote: ... B cells were stained with IgG-PE-Cy7 antibody (clone G18-145, BD Biosciences), washed twice ...
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bioRxiv - Immunology 2020Quote: The following rat anti-mouse antibodies were used for leukocyte analysis within each tissue: purified CD16/CD32 Fc blocking antibody (clone 2.4G2; BD Pharmingen™, San Jose, CA), PerCP-Cy™5.5 Ly6G antibody (clone 1A8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1) after Fc blocking and analyzed using flow cytometry (BD FACS Celesta/Novocyte Advanteon). Cells were fixed and permeabilized using intracellular staining reagents (Intracellular Fixation & Permeabilization Buffer Set ...