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Citations for Becton, Dickinson and Company :
1751 - 1800 of 8232 citations for Goat Anti Human IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... one million cells were transferred in 100 μl of FACS buffer supplemented with 0.5μM Y27632 and then added 2 μl Fc Block (BD Pharmingen, 553141) per sample followed by 10 min incubation on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in PBS + 3% FCS for cell sorting on the BD FACS Aria Fusion (BD Biosciences) at the NIHR Cambridge BRC Cell Phenotyping hub ...
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bioRxiv - Systems Biology 2023Quote: All flow cytometry raw data were saved as .fcs files and processed by the FlowJo software (version 10, BD Biosciences) for further analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... The binding of IGSF3-Fc to the cell surface was detected as the APC fluorescence intensity using FACSVerse (BD Biosciences).
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bioRxiv - Genetics 2023Quote: ... 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution: FITC-conjugated lineage cocktail with antibodies against CD4 (clone H129.19, BD Pharmingen), CD3e (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-2 and IFNγ cytokine concentrations were analyzed by sandwich ELISA according to manufacturer’s kit protocol (BD Opteia human IL-2 and human IFNγ). In vivo cerebellum lysates were analyzed for cytokines similarly ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were treated with human FcR blocking serum (BD Bisociences), fixed in 4% formaldehyde (Sigma ...
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bioRxiv - Immunology 2022Quote: Human peripheral blood was collected in EDTA tubes (BD Vacutainer) from healthy volunteers enrolled in University of Maryland Institutional Review Board (IRB ...
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bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest ...
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bioRxiv - Microbiology 2021Quote: Recombinant human TNF-α (Cat. # 554618) was purchased from BD. Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... and remaining cells were stained for human CD45 (BD 557748). Percent hCD45+ cells in the blood was determined with flow cytometry.
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bioRxiv - Cell Biology 2022Quote: Cryo-preserved primary human hepatocytes (PHH) from single donors (BD Gentest ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used on human experiments were: BCAM (566661, BD), KRT5 (ab52635 ...
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bioRxiv - Neuroscience 2022Quote: ... human BDNF (10 ng/ml, 248-BD, R&D Systems) and human NT-3 (10 ng/ml ...
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bioRxiv - Physiology 2023Quote: ... We purchased human recombinant TNFα (10 ng/ml) from BD Biosciences (San Jose ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies for human cell analysis: EpCAM-FITC (BD Biosciences, #347197), PD-L1-PE (eBiosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with human recombinant epidermal growth factor (EGF; BD Biosciences), human recombinant basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2023Quote: Human blood samples were collected in EDTA tubes (BD Biosciences). Peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Neuroscience 2023Quote: ... and Mm x Human MSH2 (1:300 BD Biosciences, #556349) or Mm x Human MSH3 (1:300 BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... or Mm x Human MSH3 (1:300 BD Biosciences, #611390) by incubating overnight at +4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or human MCP-1/CCL2 Flex Set (558287; BD Biosciences). Three hundred events per analyte from the live gate were collected on a BD FACSAria instrument (Becton Dickinson) ...
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bioRxiv - Immunology 2023Quote: ... while human cells were incubated with CD20 (2H7, BD Biosciences), CD19 (SJ25C1 ...
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bioRxiv - Physiology 2021Quote: ... goat polyclonal antibody and CD56-PE (clone B159, BD Pharmigen) monoclonal antibody in PBS 2% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were resuspended in 500 m 2% FBS in HBSS and incubated on ice in the dark with 1 l Fc block (BD Biosciences) for 3 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then resuspended in 200 uL FACS buffer and transferred to 96 well U bottom plates for staining with Fc block (BD Biosciences), zombie fixable viability stain for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... Fc receptors were blocked as described above and cells were stained with CD3e FITC (1:200, clone 145-2C11, BD Pharmingen) or CD3e PE-Cy7 (1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated for 15 minutes at room temperature in the dark in 50uL PBS with Fc Block (1/100 dilution, BD, 564220) and Zombie UV dye (1 in 500 dilution ...
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bioRxiv - Immunology 2021Quote: ... Cells were aliquoted to a count of 1 million cells each and incubated on ice with Fc Block (BD, Table S1) at a 1:20 dilution ...
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bioRxiv - Immunology 2020Quote: ... For flow cytometry analysis first Fc receptors were blocked using α-CD16/CD32 antibody (clone 2.4G2, BD Biosciences, Bedford, MA, USA). The cells were subsequently washed and stained for following surface markers with directly conjugated antibodies against CD19/B220 (PerCP Cy5.5 or eFluor 450) ...
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bioRxiv - Immunology 2019Quote: ... 10 ml PBS with 10 % FCS (buffer A) was added and the suspension was passed through a 100 µm cell strainer (BD Biosciences). The resulting single cell suspension was pelleted and resuspended in 1 ml ACK buffer (0.15 M NH4Cl ...
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bioRxiv - Immunology 2020Quote: ... CR3022 IgGs and recombinant hACE2-Fc at their respective EC80 concentrations through flow cytometric analysis on the BD FACS Canto II (BD Biosciences). Binding signal was normalized and calculated as a percentage of the binding signal to reference WT RBD-SD1 ...
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bioRxiv - Immunology 2019Quote: ... and CD3+CD4+CD25− cell population (Tconv) were directly sorted into tubes with 500 μL FCS on a FACSAria™ III (BD). For RNA sequencing ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 4% heat-inactivated FCS containing FACS buffer and data were acquired by a Symphony Flow Cytometer (BD Biosciences). Flow cytometry and t-SNE analyses were performed with FlowJo software (FlowJo10.5.3) ...
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bioRxiv - Immunology 2020Quote: Cells isolated from the organs indicated in figure 4 and figure S9 were incubated with an Fc blocking agent (BD Pharminogen) for 10min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The bacterial pellet was resuspended in 1X PBS + 0.25% BSA + 5% NGS and stained with Fc Block (BD Biosciences, clone 2.4G2), followed by SYTO BC (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed twice with PBS containing 2% FBS and were blocked with CD16/32 Fc block (Cat. 553142, BD Bioscience) before the analysis ...
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bioRxiv - Immunology 2020Quote: ... Up to 5×106 cells were washed in cold FACS wash (PBS supplemented with 2% FCS and 2mM EDTA) prior to being stained with a surface receptor antibody cocktail containing FcBlock (BD, 2.4G2) and LIVE/DEAD fixable blue dead cell stain (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: For 3T3 NIH fibroblasts cells infected with C/EBPα-ER-IRES-hCD4 (WT and T35A) were incubated with 0.1µg per 1 million cells of both Fc block and hCD4-Biotin antibody (BD Pharmingen, Cat#555347) for 10 and 20 minutes respectively ...
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bioRxiv - Immunology 2022Quote: ... and were resuspended in Fc stain buffer prior to performing flow cytometry analysis on a LSR Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software (version 10.7.1) ...
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bioRxiv - Immunology 2022Quote: ... For quantification of the different developmental steps in T cell maturation the cells were blocked with murine Fc-block (BD Biosciences) and stained with either CD45-APC/Cy7 (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 parasites were collected and incubated with VSG2WT antisera (1:4000) or VSG11WT (1:1000) together with Fc block (1:200, BD Pharmingen) in cold HMI-9 without FBS for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... CD45—CD38—CD235a—CD71— non-hematopoietic cells were sorted in DMEM supplemented with 10% FCS using a FACSAria III (BD Biosciences) and BD FACSDiva version 5.0 (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: Unfractionated BM cells were counted on the XP-300-Hematology Analyzer (Sysmex Corporation) and then Fc-blocked either with purified CD16/32 (BD Biosciences), or stained with CD16/32 antibody conjugated to a fluorophore ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were pre-incubated for 15 minutes in a staining solution (PBS+/+, 2% FBS) containing 1 μg/mL Fc block (BD Biosciences), followed by an incubation with the antibodies for 45 minutes (Table S1) ...
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bioRxiv - Immunology 2023Quote: ... at 1:600 antibody to cell suspension volume ratio in 30×106 cell/ml HBH with Fc block for 15-30 min on ice and then sorted with a BD FACS Aria III (BD Biosciences) with assistance from the University of Washington Pathology Flow Cytometry Core Facility ...
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bioRxiv - Biophysics 2022Quote: ... The FITC ERF values were assigned to both 2 μm and 6 μm beads using a specific lot of FITC-FC Bead (BD Biosciences) as a calibrator with known ERF value ...
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bioRxiv - Cancer Biology 2023Quote: ... and 7AAD-CD45+CD34-CD117-CD33- non-myeloid (lymphoid) fraction were sorted in DMEM supplemented with 10% FCS using a FACSAria III (BD Biosciences) and BD FACSDiva version 5.0.
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bioRxiv - Biochemistry 2023Quote: ... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
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bioRxiv - Immunology 2023Quote: ... Cells were acquired in BD LSR-II flow cytometer using FACS diva software and FCS files were analyzed by the Flowjo v10.5.0 software (BD Biosciences, CA, USA). For absolute counting ...