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Citations for Becton, Dickinson and Company :
1701 - 1750 of 1884 citations for Recombinant Human Aldehyde Dehydrogenase 3 Family MemberA1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed 3 times in PBS and analyzed by flow cytometry using a Symphony A5 cytometer (BD Biosciences). Data was analyzed with FlowJo software and graphed on GraphPad Prism 9 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then washed at least twice with FACS buffer (0.3% BSA + 0.02% Sodium Azide) followed by analysis (Bei et al, 2010; Theron et al, 2010) on BDverse (BD biosciences).
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bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
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bioRxiv - Genetics 2022Quote: The sorted cells were collected in 36 (3 samples × 12 bins) 5-mL polystyrene round-bottom centrifuge tubes (BD Falcon), each of which contained 500 μL of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and resuspended in PBS + 3% FCS for cell sorting on the BD FACS Aria Fusion (BD Biosciences) at the NIHR Cambridge BRC Cell Phenotyping hub ...
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bioRxiv - Immunology 2023Quote: ... and 3 μg/ml anti-IL-4 in the presence of plate bound anti-CD3ε (1 μg/ml) (BD Biosciences) and anti-CD28 (3 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Neuroscience 2023Quote: ... we created a dural eyelet by inserting a short guide tube (3-4 mm length of 27G hypodermic needle, BD) 1-2 mm into the dura and then carefully threading the probe through the eyelet under magnification ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellets from each isolate were then resuspended in 1 ml of T-R minimal medium containing no carbon source and injected into the IsoCaRB system simultaneously using a 3 ml syringe (BD Biosciences #309657 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (3 × 105) in 300 mL of serum-free medium were seeded into FluoroBlock TM Cell Culture inserts (BD Falcon). Serum free media with 100 ng/mL LOX ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following primary antibodies were used in this study: anti-active Caspase-3 (rabbit, 1:500 dilution, BD Pharmingen 559565); anti-phospho histone H3 (pH3 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed twice with R10 to remove any remaining viral particles and sorted for mCherry-positivity on a FACSAria 3 cell sorter (BD). The heterogeneous population was then assayed for successful editing by sequencing the genomic DNA flanking the Cas9 cut site ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco, BD Bioscience ...
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bioRxiv - Developmental Biology 2024Quote: ... Residual buds were dissected down to Stage 3-4 in water using a needle (BD Micro-FineTM IV Insulin Syringes). Inflorescences were moved to fresh media plates and transferred to the growth chamber for at least 24 hours for recovery from dissection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After 48h cultures were diluted and plated on Nutrient Agar plates (5 g/L peptone BD difco, BD Bioscience; 3 g/L yeast extract BD difco ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed with BD Cytofix Fixation Buffer and were stained with PE Rabbit Anti-Active Caspase-3 (BD Biosciences) in BD Perm/Wash Buffer (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were decorated with the following primary antibodies diluted in a 3% BSA solution for 1h30min at room temperature: mouse monoclonal anti-TIM23 (BD Transduction Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies used for the flow cytometry analysis of the cell surface markers were: anti-human CD11b (740965, BD OptiBuild™, 1:100), anti-human CD14 (11-0149-42 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were incubated with a fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD31 antibody (1:50; BD Pharmingen, San Diego, CA, USA) for 30 min at room temperature followed by 3 washes with phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended in sorting buffer (2% FBS and 1% Penicillin-Streptomycin in DMEM) and blocked with human FcR blocking reagent (Cat#564220; BD; used 1:50) for 10 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and the purity of monocytes was evaluated by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Antibody concentrations were as follows: alveolar macrophage membranes were stained using CD206 PE-CF594 (Mouse Anti-Human CD206, BD Biosciences; catalogue number: 564063) at a concentration of 4µL in a 50μL final staining volume per section ...
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bioRxiv - Cell Biology 2019Quote: The following antibodies were used for fluorescence microscopy: mouse IgG2B anti-human DC-SIGN at 2 μg/ml (DCN46, BD Biosciences, Breda, Netherlands), mouse IgG1 anti-human AZN-D1 at 2 μg/ml (Geijtenbeek et al. ...
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bioRxiv - Immunology 2021Quote: NK cells and cancer cells were cocultured at 10:1 ratio and supplemented with 20ul of PE mouse anti-human CD107a antibody (BD Pharmingen™, #555801) in a total volume of 220ul in a 96 well plate at 37C incubator for 90 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated for 45 min with the indicated antibodies: AlexaFluor® 647 anti-human CD107a antibody (diluted at 1/100, clone H4A3; BD Pharmingen TM), anti-human Perforin (10µg/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in 50 µL Perm/Wash buffer containing a cocktail of anti-human antibodies including IFNγ BV480 (BD, 566100, clone B27), TNFα BV605 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Human platelets in the obtained blood were identified using APC-labeled polyclonal rabbit anti-human (h) CD41 and/or CD42b (BD Pharmingen, 1:100) and fluorescein isothiocyanate (FITC)-labeled polyclonal rabbit anti-mouse (m ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 μL per 1x10e6 cells) with human IgG-Fc block (1:100) followed by the incubation with streptavidin-magnetic bead particles (BD Biosciences, Ref#557812) at 50 μL per 1x10e6 cells ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Bioengineering 2024Quote: ... and stained at room temperature for 1 hour using a PE labeled mouse anti-IDO1 antibody (12-9477-42, Thermo, Waltham, MA) or a BV786 labeled mouse anti-human HLA-DR antibody (564041, BD, Franklin Lakes, NJ) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Infected cells grew for 3 days and then infection efficiency was measured using flow cytometry to measure EGFP (BD Accuri C6); the dose that gave rise to 5 - 15% EGFP+ cells was selected for each LSR for further experiments ...
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bioRxiv - Cell Biology 2020Quote: ... Percentage of platelets in BALF (n=3-6) was quantified on a BD FACSVerse flow cytometer (BD Biosciences, San Jose, USA) and analysed with FlowJo v10 software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... 1–3 ×106 PBMC were stained with a saturating concentration of the following antibodies: CD3 AF700 (clone SP34-2, BD Biosciences), CD4 eFluor450 (clone OKT4 ...
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bioRxiv - Microbiology 2022Quote: ... BW25113ΔompT (JW0554) and BW25113ΔeptA (JW5730) (Supplementary Table 1) were routinely grown aerobically in Difco Antibiotic Medium 3 (BD, Franklin Lakes, USA) at 37 °C degrees without agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in cold phosphate-buffered saline (PBS) containing 3% FBS and stored at 4°C until analysis using a LSRII flow cytometer (BD Biosciences). FlowJo software v10 (Treestar ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were either stained with antibody dissolved in PBS containing 3 % albumin (surface markers) or permeabilized with Perm/Wash buffer (BD Biosciences) according to manufacturer’s instruction and stained with the respective antibody (mix ...
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bioRxiv - Immunology 2020Quote: ... then cultured in complete RPMI media (3×106 cells/mL) plated on anti-CD3 (145-2C11; BD Pharmingen, San Diego, CA) antibody-coated tissue culture plates ...
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bioRxiv - Immunology 2019Quote: ... stained with cocktails of fluorochrome–conjugated antibodies (Supplemental Table 3), and acquired on LSR II (Cohort 1) or CANTO II (TT0, T6M) flow cytometers using FACS DIVA software (BD Biosciences). All flow cytometry data were analyzed with FlowJo (TreeStar Software ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Biochemistry 2019Quote: ... and four patients with cardiac comorbidities including severe congestive cardiac failure (1 male, 3 female, 29-59 years old) was drawn into ACD-A tubes (BD Vacutainer) and plasma collected by twice centrifugation at 800 g for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: SUIT2 cells (4 × 106) and A549 cells (3 × 106) were dispersed in 75 μL DMEM and 75 μL Matrigel (356230, BD Biosciences) and injected subcutaneously into nude mice ...
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bioRxiv - Pathology 2019Quote: ... were added in triplicate for each experimental group to the 96-well plates pre-coated with 3 μg/ml anti-CD3 antibody (clone HIT3a, BD Biosciences) for 3-5 h at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 1 mL plastic syringes (BD PlastipakTM; sterile needles, 25G x 1” – NR. 18, 0.5 mm x 25 mm, BD MicrolanceTM 3), and portex tubing PE (Scientific Laboratory Supplies ...
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bioRxiv - Bioengineering 2020Quote: ... cells were then suspended in alginate precursor solution ‘A’ at 5 x 106 cells/mL (final concentration 2.5 x 106 cells/mL) and loaded into 1 or 3 mL syringes (BD luer lock). Following previous protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed with PBS containing 5 mM EDTA and 3% FBS (FACS Buffer) and then blocked with 1% anti-mouse CD16/CD32 (BD biosciences), 5% normal rat serum and 5% normal mouse serum in FACS buffer for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell clusters were washed 3 times with ice-cold HBSS containing 5% FBS and filtered through 100-μm cell strainer (BD Biosciences). The cell suspension containing acini was carefully layered on top of HBSS containing 30% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... A 1.0 mL aliquot consisting of approximately 108 cells was inoculated in a 100 mL 3% tryptic soy broth (TSB, BD-Difco) in Erlenmeyer flasks ...
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bioRxiv - Microbiology 2020Quote: ... AU) of the infected cells 1 and 3 hours after infection by flow cytometry using a FACS Aria III (BD Biosciences). The intensity of GFP fluorescence corresponds to the amount of intracellular bacteria ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining using antibodies detecting Active Caspase-3 (C92-605) and BCL2L11 (Y36) was performed using BD Cytofix/Cytoperm (BD Biosciences) or eBioscience FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 20µL were transferred into 180µL of 10mM Tris-1mM EDTA (for DiOC2(3) staining) or PBS (for DiSC3(5) staining) and analyzed by flow cytometry using LSR Fortessa (BD Bioscience) operated by FACS Diva software (BD Bioscience) ...