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Citations for Becton, Dickinson and Company :
1451 - 1500 of 1884 citations for Recombinant Human Aldehyde Dehydrogenase 3 Family MemberA1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 3% horse serum and mouse anti-BrdU primary antibody (1:200, BD Biosciences; 347580, San Jose, CA, USA). Visualization of BrdU+ cells was performed with Alexa 488-conjugated donkey anti-mouse secondary antibody (1:250 ...
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bioRxiv - Biochemistry 2019Quote: ... The plates were washed 3 times using PBST and then developed using an ELISA developing kit (BD Biosciences). The optical densities at 450 nm were read using an ELISA plate reader (Molecular Devices).
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bioRxiv - Immunology 2021Quote: ... 3 weeks) and single clones were sorted into 96-well plates using an IMI5L cell sorter (BD Biosciences). Clones were grown under selection for 3–4 wk and tested for KO by DNA sequencing and immunostaining and flow cytometry ...
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bioRxiv - Plant Biology 2020Quote: ... Laminar flow of buffer was buffer was verified with 0.1 % SPADNS and the chamber was treated with 0.05 % HPMC before IEF-FFE amphylites (pH 3-9; BD) were applied to inlets 2-6 of the chamber at a flow rate of 60 ml per hour ...
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bioRxiv - Microbiology 2020Quote: ... Bax and Bcl-2) and Apoptosis Sampler Kit II (for caspase-2 and caspase-3) were from BD Transduction Laboratories (Franklin Lakes ...
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bioRxiv - Microbiology 2019Quote: ... BMECs were seeded on the apical side of fibronectin-coated inserts (BD Falcon, 24-well, 3 µm pores). Concurrently ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to the column were eluted with 3 ml Elution buffer (BD buffer with 100 mM imidazole) and aliquoted in 300 μl fractions ...
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bioRxiv - Microbiology 2022Quote: ... Proteins bound to the column were eluted with 3 ml elution buffer (BD buffer with 100 mM imidazole) and aliquoted in 300 μl fractions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then transferred onto a nitrocellulose membrane which was then blocked in 3% skimmed milk (BD Difco). Membranes were then subjected to immunoblotting ...
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bioRxiv - Cancer Biology 2021Quote: ... GFP-expressing PC-3 cells were sorted by FACS Aria III (BD Life Sciences, Franklin Lakes, NJ, USA) and maintained with complete medium ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 36 cm above the arena an infrared-sensitive Chameleon 3 camera (CM3-U3-13S2C-CS-BD, FLIR, USA) was used to record fly movements ...
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bioRxiv - Immunology 2021Quote: We have used 3 BD FACSAria™ Fusion cell sorters (Fusion) and a FACSSymphony A5 (Symphony) from BD Biosciences ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed with PBS and incubated with the active caspase-3 antibody (1:500 dilution, BD Biosciences) in a 50/50 (v/v ...
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bioRxiv - Immunology 2021Quote: ... Wells were extensively washed again and developed with 3-amino-9-ethyl-carbazole (AEC) substrate set (BD Biosciences). After drying ...
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bioRxiv - Biochemistry 2020Quote: ... The sections were incubated with anti-mouse Mac-3 antibody (1:1000, BD Biosciences, Franklin Lakes, NJ, USA) and a color reaction was subsequently developed using 3.3’-diaminobenzidine (Bright-DAB ...
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bioRxiv - Immunology 2021Quote: bEnd.3 cells were cultured to 80% confluence then seeded onto transwells (8 µm pore size; BD Biosciences) and grown for 5 d until they reached polarisation ...
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bioRxiv - Cancer Biology 2021Quote: PCa cells (3 × 105) in serum-free medium were seeded into FluoroBlock TM Cell Culture insert (BD Falcon). The lower chamber contained conditioned media (CM ...
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bioRxiv - Cell Biology 2022Quote: Cells were harvested by centrifugation at 1600 rpm for 3 minutes and fixed in Cytofix Fixation Buffer (BD) for 10 minutes at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... for 3 hours at 37°C in the presence of GolgiStop (monensin, according to manufacturer’s recommendation, BD Biosciences). Cells were washed and labelled with fixable viability dye (LIVE/DEAD eFlour780 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were subsequently stained with the rabbit anti-active caspase-3 antibody (clone C92-605) (BD Biosciences, USA) in the dark ...
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bioRxiv - Immunology 2023Quote: ... Left lung lobes were homogenized in 3 mL MilliQ water containing PANTA™ Antibiotic Mixture (BD, cat.no. #245114) using GentleMACS M-tubes (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples (24 samples; 8 samples per macrophage polarization state) were cleaned (Supplementary Figure 3; FlowJo, version 10.7.1, BD), and live single cells were imported into OMIQ (Omiq.ai ...
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bioRxiv - Developmental Biology 2022Quote: ... washed and then incubated with 1:700 dilution of Rabbit Activated Caspase 3 (BD Biosciences, Franklin Lakes, NJ) in block solution overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Prokaryotic cells and virus-like particles (VLPs) from site #1 – #3 were measured on a flow cytometer (BD Accuri C6 ...
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bioRxiv - Immunology 2023Quote: ... antibody mix 3 (Supplementary Table S4) with Sample Tag oligonucleotide-conjugated antibodies (Single-Cell Multiplexing Kit, BD Biosciences) was added to the cell suspension and left on ice for 15 minutes for staining ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by overnight incubation with the primary antibody (Purified Rabbit Anti-active caspase 3 (BD 559565) 1:200 in Block solution ...
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bioRxiv - Immunology 2023Quote: ... except that mAbs to pSTAT5 and GATA-3 were prepared in 1× BD Perm/Wash buffer (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed 3 times with 1 x Permeabilization buffer (eBioscience) and fixed using Cytofix fixation buffer (BD). The antibodies used are listed in Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then sorted for mCherry-positive cells (cutoff set at 99.9th percentile of the untransduced cell population’s mCherry signal) using a FACSAria 3 cell sorter (BD). We observed 4% of cells with a mCherry positive signal ...
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bioRxiv - Developmental Biology 2024Quote: ... Red blood cells were lysed twice on ice for 3 min with BD Pharm Lyse Buffer (BD Pharmingen). Identification of leukocyte subsets was performed by staining with antibodies ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with 0.5ug/ml purified NA/LE hamster anti-mouse-CD40 (clone HM40-3 BD Pharmingen), 10ug/ml affinity pure F(ab)2 fragment goat anti-mouse-IgM ...
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bioRxiv - Microbiology 2023Quote: ... 3 loopfuls of biomass grown at 30°C on Brain-Heart Infusion (BHI) agar (BD Biosciences, NJ, USA) were collected and mixed with a phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with 1 µg/ml Doxycycline before sorting for GFP-positive cells (Aria 3 Sorter; BD). For lentiCRISPR v2 system ...
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bioRxiv - Systems Biology 2020Quote: ... White cells were resuspended in PBS supplemented with 1% human serum and 2 mM EDTA and analyzed on an LSR Fortessa instrument (BD Biosciences).
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bioRxiv - Immunology 2021Quote: Supernatants were collected 4 or 24 h post-challenge and the levels of TNF and mature IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems) ...
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bioRxiv - Immunology 2021Quote: CD47 expression or cross-dressing on cells was determined by direct staining with BV786-conjugated anti human specific CD47 mAb B6H12 (abbreviated anti-hCD47; BD Bioscience) or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus ...
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bioRxiv - Microbiology 2020Quote: Macrophages were infected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using ELISA kits for human IL-1β (BD Biosciences) and IL-18 (R&D Systems).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were re-suspended in 100 μl of FCSB and 1 μl of mouse anti-human CD10-PE (561002, BD Biosciences) and mouse anti-human CD92-AF647 (565316 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated and resuspended iTF-Microglia were blocked for 15 min with 1:20 Human FC Block (BD Biosciences; Cat. No. 564220) and then stained with 1:66 PE/Cy7 anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were added to the washed ELISA plate and incubated together with anti-human CD107a (BD, 1:40, clone: H4A3), brefeldin A (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... primary hMDMs were transfected in a 48-well plate as described above and harvested supernatants were assayed for cytokine levels using an ELISA kit for human IL-1β (BD Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... with anti-positive (CD90, CD105, CD44) and negative marker (CD34, CD11b, CD19, CD45, MHC class II) antibodies (Human MSC Analysis Kit, 562245, BD Biosciences), as described in detail in [42] ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell suspensions from APEL vascular differentiations were incubated with mouse anti-human CD31-APC (eBioscience, San Diego, CA) and CD146-PE (BD Biosciences) antibodies for 30 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... The HLA-DQ cell surface expression was performed by cytofluorimetric analysis using the FACSAria III and DIVA software with FITC mouse anti-human HLA-DQ antibody (BD Biosciences). The quantitation of specific transcripts was performed by qRT-PCR as described in [9] ...
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bioRxiv - Cancer Biology 2019Quote: ... cKIT and PDGFRA expression was assessed using mouse anti-human CD117 (PE-Cyanine7, clone 104D2, #25-1178-42, eBioscience) and CD140a (PE, clone αR1, #556002, BD Pharmingen) respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 h in 5% milk/TBS Tween-20 solution and incubated for 1 h with anti-TOP2β (1:5,000, mouse anti-human, Clone 40/Topo IIβ, RUO – 611492, BD Biosciences-US) or anti-TOP2α (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stained with a 1:50 dilution of phycoerythrin (PE)-conjugated mouse monoclonal antibody against human TRAIL (550516, BD Biosciences). For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770 ...
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bioRxiv - Bioengineering 2020Quote: ... Cytokine concentrations in the culture supernatant were measured using BD Cytometric Bead Array Human Th1/Th2 Cytokine Kit II (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Cytokines in the serum sample were measured using human Th1/Th2/Th17 cytometric bead array kit (BD Bioscience, San Jose, CA) according to the manufacture’s instruction ...