Labshake search
Citations for Becton, Dickinson and Company :
1651 - 1700 of 2278 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... the pLCOs in suspension were centrifuged at 500 × g for 5 minutes at 4 °C and then resuspended in cold growth factor-reduced Matrigel (BD Biosciences). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... digested lung cells were resuspended in 400 μl of buffer with 5 μl of anti-CD31-FITC (BD Biosciences, CA, USA), 5 μl of anti-CD45-APC (BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
-
bioRxiv - Cancer Biology 2024Quote: ... at 9:00 am 5 mL of fasted blood from the antecubital vein was collected into an EDTA Vacutainer tube (BD, 366643), then centrifuged at 800 x g for 10 min at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Cas9-BFP+ cells were sorted for top 5% of the brightest GFP signal simultaneously on two BD FACSAria II Cell Sorters (BD Biosciences) equipped with blue 488 nm and violet 405 nm lasers ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then cultured for 2 hours at 37°C in 5% CO2 incubator and followed by the addition of protein secretion inhibitor cocktail containing brefeldin A and monensin (GolgiPlug, BD, 555029) for another 4-hours ...
-
bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
-
bioRxiv - Immunology 2022Quote: ... were re-stimulated in vitro for 5h at 37°C 5% CO2 in cRPMI supplemented with GolgiStop (1/250; BD bioscience), GolgiPlug (1/500 ...
-
bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
-
bioRxiv - Genomics 2022Quote: ... and then dounce homogenized (10 loose strokes and 5 tight strokes) and filtered through 35-40μm cell strainers (BD Biosciences, 352235). Nuclei were pelleted (600g ...
-
bioRxiv - Immunology 2022Quote: ... at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029) for the last 5 h of the incubation ...
-
bioRxiv - Microbiology 2022Quote: ... 50 μL of DEs were diluted into 500 μL of FACS diluent buffer (PBS + 1% Tween-20) in a 5 mL 12 × 75 mm round bottom FACS tube (BD Biosciences). After standard autocalibration on the Sony SH800 ...
-
bioRxiv - Bioengineering 2023Quote: ... and then cells were re-suspended in 150 μL of PBS with 5% FBS and analyzed on a BD FACS Canto2 flow cytometer (BD Biosciences) for PE-Cy7 expression.
-
bioRxiv - Cell Biology 2022Quote: ... Samples were stimulated with the indicated agonists and labelled with a PS detecting antibody (CyTM 5 Annexin V, #559934, BD Biosciences) for 15 min at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... The following primary antibodies were used: Anti-CD166 mouse monoclonal antibody conjugated with PE (phycoerythrin) at 5 uL (BD Biosciences #559263), anti-LRIG1 sheep polyclonal unconjugated antibody at 2.5 ug (Invitrogen #PA5-47928) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were acquired on a Cytek Aurora spectral flow cytometer using Cytek SpectroFlo software (Cytek; the HD BM samples) or a LSR Fortessa X20 (special order research product with 5 lasers; BD Biosciences) and analyzed using FlowJo software (v10.8.1 ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were discarded and cells resuspended in FACS buffer and tetramer-binding B cells were individually sorted using a 5-laser (355nm, 405nm, 488nm, 561nm, 640nm) FACS Aria (BD Biosciences) into empty 96-well low profile PCR plates (Labcon) ...
-
bioRxiv - Immunology 2024Quote: ... Data was acquired using a Cytek Aurora 5-laser flow cytometer or ImageStream cytometer (CytekBio) and subsequently analyzed FlowJo V10 (BD Biosciences).
-
bioRxiv - Immunology 2024Quote: ... Cells were spun down at 300 g for 5 min and supernatant was collected for analysis by mouse inflammatory cytokine bead array (CBA, BD Biosciences). Cells were resuspended and stained for CD4 and analyzed for proliferation based on CFSE decrease (Fig SI 3).
-
bioRxiv - Immunology 2024Quote: ... 50 μl of PBS-BSA containing 2.5 μl of CD11c APC antibody (clone S-HCL-3) or 5 μl of CD11c BV421 antibody (clone B-ly6 RUO) (BD Biosciences) were added to each tube ...
-
bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 20 minutes at 4°C in FACS buffer containing 10% normal goat serum ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then cultured at a density of 5-20k live B cells/well in 96-well Microtest U-bottom plates (BD Falcon)for 8 days with 100 ng/mL anti-human CD40 antibody (clone G28.5 ...
-
bioRxiv - Immunology 2024Quote: ... cultured pMacs in 24-well plates were incubated in primary anti-MHC-II monoclonal antibody (mAB; BD Biosciences, see Table 5) at a final concentration of 5μg/mL for 1 hour at 37°C in FACS buffer to allow for the antibody-binding and subsequent uptake of MHC-II complexes ...
-
bioRxiv - Microbiology 2024Quote: ... and its mutants were all grown at 37°C with 5% CO2 with either Todd-Hewitt medium (TH medium; BD Biosciences), chemically defined media (CDM) ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in 1X PBS with 1X protease/phosphatase inhibitor cocktail in and filtered through a 5 ml cell-strainer capped FACS tube (BD Falcon). Cell sorting was performed on BD Biosciences FACS Aria Fusion ...
-
bioRxiv - Immunology 2024Quote: ... and each tube was stained with 5 μL of APC-Cy7 Mouse Anti-Human CD31 (PECAM-1) antibody (clone WM59, BD Biosciences) and 5 μL of APC anti-human CD144 (VE-Cadherin ...
-
bioRxiv - Molecular Biology 2024Quote: ... were stimulated by incubation with 5 µg ml-1 anti-IgM F(ab’)₂ fragment antibody (115-006-020; Jackson Immuno Research) with or without 5 µg ml-1 anti-CD40 antibody (553787, BD Pharmingen), with 100 ng/ml MPL-A (Invivogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... Xenografts were established by subcutaneous injections of PC-9 cells expressing ERBB4 variants (5 x 106 cells in 50% Matrigel matrix (#354234, BD Biosciences)) in the right flank of each mouse ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were analysed on the BD LSR Fortessa X-20 or BD FACSymphony or sorted on the BD FACSAria Fusion 5 or BD FACSAria Fusion 3 (BD Biosciences) at the Peter MacCallum Cancer Centre flow cytometry core facility ...
-
Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... NK cells were transfected with 1-2μg EGFP mRNA-LNP per 106 cells on day +7 in upright culture flask at 37°C in a humidified incubator with 5% of CO2 and then harvested for flowcytometry analysis with BD FACSLyric system (BD Biosciences). Optimal transfection conditions were determined using EGFP mRNA-LNP expression results ...
-
bioRxiv - Cancer Biology 2024Quote: ... and monocyte block for 5 minutes prior to staining with the 20-antibody cocktail (Supplemental Methods) in Brilliant Stain Buffer Plus (BD Biosciences) and FACS buffer (PBS ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... was taken after 5 hours (day 0) and strain ratios were immediately measured using a flow cytometer (HTS, Fortessa, BD Bioscience). Cultures were then propagated with 1:1000 dilutions into fresh medium every 24 hours for other 2 days ...
-
bioRxiv - Immunology 2021Quote: ... 558121), CD11c (clone 3.9, 748288), CD3 (clone SP34-2, 557757) and ITGB7 (clone FIB504, 555945) were purchased from BD Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: Primary human bronchial epithelial cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
-
bioRxiv - Bioengineering 2022Quote: Cells were fixed in 2% PFA for 15 minutes and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with either conformation specific BAK antibody (Clone# G317-2, 1:100) (Cat# 556382, BD Pharmingen) followed by phycoerythrin (PE)-conjugated anti-mouse antibody (1:200 ...
-
bioRxiv - Immunology 2020Quote: ... cells were fixed/permeabilized using BD FACS lysing solution and permeabilizing solution 2 according to manufacturer’s instructions (BD Biosciences) followed by intracellular staining with Ki-67-PE and t-bet-BV711 (Biolegend) ...
-
bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
-
bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
-
bioRxiv - Microbiology 2021Quote: ... Intracellular staining was performed using rat anti-mouse IFN-γ-PECy7 (clone XMG1-2, 1:300, BD cat#557649), rat anti-mouse IL-2-PE (clone JES6-5H4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CWR22Rv1 xenografts were established in the flanks of mice by injecting ~2 million cells in 50% matrigel (BD Biosciences). When tumors reached approximately 200mm3 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in blocking buffer (PBS with 2% FBS) containing an anti-BrdU monoclonal antibody (BD Biosciences) (1:100 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were sorted at a concentration of 2 million cells per mL using FACS- Aria-III (BD Biosciences). Forward and side scatter parameters were adjusted so as to eliminate cell clumps and debris ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in 2% fetal-bovine serum in PBS and analyzed using the LSRFortessa flow cytometer (BD Biosciences). A total of 20,000 events were collected for each sample ...
-
bioRxiv - Immunology 2022Quote: ... All experimental steps were performed in a 50/50 mixture of PBS + 2% FBS and Brilliant Buffer (BD Bioscience). Antigen probes for Spike ...
-
bioRxiv - Developmental Biology 2022Quote: PI-annexin V apoptosis assay was performed at differentiation week 2 and week 4 in normal and HGPS iPSCs-keratinocytes induction according to the manufacturer’s instruction (BD). In brief ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2×105 cells were washed with PBS1X and resuspended in 100 µL of Annexin-V binding buffer (BD Biosciences). 1 µL of Annexin-V-APC (Cat# 550474) ...
-
bioRxiv - Immunology 2020Quote: ... T cells and DC were stained with anti-syndecan-1 (rat IgG2Aκ monoclonal antibody, clone 281-2, BD Biosciences) or isotype control (rat IgG2Aκ ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2% FBS) and analyzed for green and red fluorescence by flow cytometry using a BD FACSymphony instrument (BD-Biosciences).