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Citations for Becton, Dickinson and Company :
1601 - 1650 of 2116 citations for 2 Amino 4 bromobenzyl alcohol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... subtilis strains were grown in 2×SGG [16 g/L nutrient broth (BD Difco, Franklin Lakes, NJ, USA), 1% glycerol ...
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bioRxiv - Immunology 2024Quote: ... Serum total IgE concentration was determined from a standard curve of mouse IgE (clone C38–2; BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... CD31 (PECAM-1) antibody (clone MEC 13.3) and to CD102 (ICAM-2) antibody (clone 3C4) were from BD Pharmingen ...
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bioRxiv - Genomics 2020Quote: ... TFA4 strain was cultivated in nutrient Zobell medium (4 g L−1 peptone and 1g L−1 yeast extract Becton, Dickinson and Company, Sparks ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then left at 4°C overnight in a blocking solution containing the primary antibody: mouse E-Cadherin (BD Biosciences, 1:1000) and mouse N-Cadherin (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were allowed to recover for 2 days in 6-well plates filled with 4 ml medium per well before being FACS sorted for mCherry-expression on a BD FACS Aria III (BD Biosciences, Heidelberg, Germany). For subsequent limiting dilution cloning ...
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bioRxiv - Immunology 2021Quote: Control and SC-macrophages were fixed with 4% PFA for 10 mins and permeabilized with 1x perm wash buffer (BD cat#51-2091KZ) for 30 mins at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were incubated overnight at 4 °C with primary mouse monoclonal anti-alpha synuclein antibody (Cat no. 610787; BD Biosciences; 1:200) and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no ...
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bioRxiv - Immunology 2022Quote: ... were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878, respectively), and were exposed to various stimuli (e.g ...
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bioRxiv - Microbiology 2021Quote: ... a 10 µl inoculating loop was used to streak cecal slurry (cecal contents in BPW) onto Xylose Lysine Tergitol-4 agar (BD Difco, Sparks, MD). supplemented with 4 ppm tetracycline for trial 1 and BGS supplemented 32 ppm ampicillin (amp ...
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bioRxiv - Immunology 2020Quote: ... Pellets were then stained with a panel of antibodies for 30 minutes (4°C) followed by washes in PBS before analysis by flow cytometry (BD, Franklin Lakes, NJ).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated in the FC wash buffer (2h at 4 °C) containing FITC-coupled anti-CD44 antibody (1 to 80 BD Pharmingen Cat.No. 555478) or its isotype control (FITC Mouse IgG2b ...
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bioRxiv - Neuroscience 2020Quote: Brains from paraformaldehyde-perfused 12 month-old mice were sectioned and 30 µm free-floating sections were incubated overnight at 4°C with primary antibodies (anti-αsyn, BD Transduction, 1:700), anti-DNAJC5 (Millipore ...
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bioRxiv - Immunology 2020Quote: ... Cells were then spun down at 500 × g for 5 min at 4°C and resuspended with the following antibody-conjugated fluorophores for 20 minutes at room temperature: anti-CD3-BV421 (BD, 1F4, 1:100), anti-CD4-PE-Cy7 (BD ...
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bioRxiv - Systems Biology 2021Quote: ... HeLa and RAW 264.7 cells were washed by chilled PBS and sorted on the nanoPOTS chips (4 × 12, 1.2 mm diameter per well) using the Influx II cell sorter (BD Biosciences, San Jose, CA) as described previously 14 ...
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bioRxiv - Immunology 2020Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... overnight and then stimulated with 1 nM N4 in the presence of vehicle or Compound 182 (1 μM) for 4 h at 37°C in complete T cell media supplemented with BD GolgiPlug™ and BD GolgiStop™ (BD Biosciences). Cells were then stained with fluorophore-conjugated antibodies to cell surface CD8 and intracellular IFNγ ...
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bioRxiv - Immunology 2023Quote: pLNs were isolated from 6-week old female NOD mice and fixed for 14h at 4°C in BD Cytoperm/Cytofix (BD Bioscience, Cat#: 554722) diluted 1/4 in PBS ...
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bioRxiv - Microbiology 2022Quote: ... the individual co-cultures were 10-fold serially diluted with 1X anaerobic phosphate buffer saline (PBS) and plated on Xylose Lysine Tergitol 4 (XLT4) agar (BD Difco, Houston, TX). The plates were incubated aerobically at 37°C for 24 hours followed by plating on XLT4 agar and colony forming units (CFU ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2023Quote: Cells were first labelled with Fc block for 10 minutes at 4°C and then the following antibodies added: CD45-PE (BD, 30-F.11), Siglec F-APC (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated at 4°C for 15 min with Fc Block (Rat anti-mouse CD16 and CD32; BD Pharmingen – Cat.553141) and 30 min with the antibody mixes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667, 1:100 in 1X BD Perm/wash solution) or anti-BST2 (Proteintech #13560-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was washed with 3-4 ml washing buffer which was fresh PBSB 1% and then was filtered through a 70-µm cell strainer (BD Bioscience, NY, USA). Then using a long tube ...
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bioRxiv - Genomics 2023Quote: ... and half of the remaining chromatin was rotated overnight at 4°C with 4.5 µg of anti-p53 (BD Biosciences #554293, Lot #9046678). The other half of the remaining chromatin was also rotated overnight as a no-antibody control ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were blocked for 5 min at 4 °C with rat anti-mouse CD16/CD32 (#553142, Mouse BD Fc Block, BD Biosciences) in FACS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-human CD19 (Becton, Dickinson and Company, BD ...
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bioRxiv - Immunology 2023Quote: ... Note these experiments were carried out using early access kits from BD Genomics before the implementation of commercially-available single-cell protein/RNA assays (e.g. Feature Barcoding, 10x Genomics; BD Abseq, BD Genomics, Supplemental Table 4), and researchers are recommended to use those newer solutions for any follow-up studies as the techniques and reagents have been refined ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were washed with PBS and stained for 20min at 4°C with anti-EphB2 APC conjugated antibody (BD Biosciences, Heidelberg, Germany). Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated around the rim of the well of a 96-well plate in a 4:3 mixture of Matrigel and MammoCult medium (BD Bioscience CB-40324). The cell mixture was then incubated at 37°C with 5% CO2 for 15 min to solidify the gel ...
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bioRxiv - Immunology 2022Quote: ... mice were euthanized and tumors were quickly harvested and fixed for 14-16 hr at 4&C in BD Cytofix/Cytoperm (BD Bioscience, Cat #: 554722) diluted 1:4 in PBS ...
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bioRxiv - Immunology 2022Quote: ... In-between incubation steps, cells were washed (300 x g, 10 min, 4°C) with 40 mL of BD Cell Wash (BD Biosciences, Allschwil, Switzerland). In order to block Fc receptors ...
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bioRxiv - Microbiology 2022Quote: ... 98 mL of fresh MRS medium is then inoculated with 2 mL of the overnight culture and incubated at 37°C without shaking to an OD of 0.5-0.6 (ca. 4-5 hours) in a GasPak anaerobic jar (BD GasPak™ EZ container systems). Cells are harvested a first time by centrifugation at 4,000 g for 5 min ...
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bioRxiv - Systems Biology 2022Quote: Single cell suspensions were plated around the rim of the well in the 3:4 mixture of Mammocult and Matrigel (BD Bioscience CB-40324). We seed 5’000 cells/wells for mini-rings in white 96-well plates (Corning #3603 ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the samples were fixated and permeabilized for 20 min and 60 min at 4 °C in the dark using BD Cytofix/Cytoperm™ solution and BD Perm/Wash™ solution (BD Biosciences), respectively ...
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bioRxiv - Developmental Biology 2023Quote: A total of 4 ml of blood were withdrawn and transferred to BD Vacutainer™ Hemogard closure plastic K2-EDTA tubes (BD 367525, BD). Tubes were inverted 10 times to ensure blood and EDTA were well mixed and kept at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... TCR-engineered T cells containing 5×104 transgenic TCR (mTRBC)+ cells were co-cultured with 5×104 peptide-pulsed K562 cells for 4 h at 37°C in cRPMI and in the presence of 1 μL/mL GolgiPlug™ (BD Biosciences, 555029). As a positive control ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310 ...
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bioRxiv - Bioengineering 2024Quote: ... 1:20 BD Pharmingen™ Alexa Fluor® 488 Mouse anti-SSEA-4 Clone MC813-70 (RUO) (BD Biosciences, Franklin Lakes, NJ, USA) 2 ml/well solution ...
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bioRxiv - Bioengineering 2024Quote: ... for 60 min at room temperature and then stained with anti-human/mouse phospho-STAT3-AF647 (clone 4/P-STAT3, BD Biosciences, 1:100) or anti-human/mouse phospho-SMAD2/3-AF647 (clone 072-670 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell samples were stained with the following antibodies in cell staining buffer for 40 min at 4 °C: anti-mouse-CD3e-FITC (553061, BD Biosciences, 1:200), anti-mouse-CD4-PercpCy5.5 (550954 ...
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bioRxiv - Immunology 2021Quote: ... 558121), CD11c (clone 3.9, 748288), CD3 (clone SP34-2, 557757) and ITGB7 (clone FIB504, 555945) were purchased from BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Primary human bronchial epithelial cells (Passage 1 or 2) were expanded on flasks coated with collagen I (BD Biocoat) in bronchial epithelial growth medium (BEGM) ...
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bioRxiv - Bioengineering 2022Quote: Cells were fixed in 2% PFA for 15 minutes and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with either conformation specific BAK antibody (Clone# G317-2, 1:100) (Cat# 556382, BD Pharmingen) followed by phycoerythrin (PE)-conjugated anti-mouse antibody (1:200 ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed/permeabilized using BD FACS lysing solution and permeabilizing solution 2 according to manufacturer’s instructions (BD Biosciences) followed by intracellular staining with Ki-67-PE and t-bet-BV711 (Biolegend) ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences, Custom Bulk 624040, PerCP-Cy5.5; BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
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bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular staining was performed using rat anti-mouse IFN-γ-PECy7 (clone XMG1-2, 1:300, BD cat#557649), rat anti-mouse IL-2-PE (clone JES6-5H4 ...