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Citations for Becton, Dickinson and Company :
1751 - 1800 of 2116 citations for 2 Amino 4 bromobenzyl alcohol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... E.coli strain Rosetta™ 2(DE3)pLysS bearing pET21d-FGF10 was grown in 2x YT medium (BD Biosciences, Cat#244020) with Carbenicillin (50µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Collagenase activity was quenched by dilution with FACS buffer (PBS, 0.5% FBS, 2 mM EDTA) and digestion products were filtered through 70 µm nylon filters (BD Biosciences). Cells were again washed with FACS buffer and immediately processed for immunostaining first by blocking Fc receptor binding with rat anti-mouse CD16/CD32 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Flow cytometry profiles of the HSPC compartment (Extended Data Fig.2, Fig.9) were analysed using FlowJo software (version 10.1, BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 2 ml of FACS buffer and fixed by adding 3.5 ml of fixation/permeabilization buffer (BD Biosciences) and rocking overnight at 4C ...
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bioRxiv - Pathology 2021Quote: ... Whole blood samples were then lysed and fixed in 2 ml of FACS Lysing Solution (BD Biosciences, San Jose, CA) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained with antibody (Supplementary Table. 2) and LSK cells were isolated through cell sorting using FACSAria (BD). Sorted LSK cells were cultured in StemSpan SFEM serum free media (stemcell technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Pre-coated coverslips were printed by stamping (0.2 mm spot diameter and 0.5 mm spot interspace) with a cell-attachment mixture of rat tail collagen (BD Biosciences), poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... After staining for 45 min at 4°C cells were washed and taken up in 200 µl PBS+2%FBS and 1/1000 7AAD before being run on a LSR Fortessa (BD). Lineage out-put was analysed in FlowJo ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Biochemistry 2022Quote: ... washed and resuspended in FACS buffer (2% FBS in 1X PBS) and filtered through filter-topped flow tubes (BD falcon) using a luer-lock syringe at 2×106 cells/ml ...
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bioRxiv - Microbiology 2020Quote: ... cells were resuspended and 2-43 binding to cell-surface S trimer was quantified on LSRII flow cytometer (BD Biosciences). The mean fluorescence intensity of APC in GFP-positive cells was analyzed using FlowJo and the relative binding of 2-43 to S trimer in the presence of competitors was calculated as the percentage of the mean fluorescence intensity compared to that of the competitor-free controls.
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bioRxiv - Immunology 2020Quote: ... Then cells were fixed with 2% paraformaldehyde for 1 h prior to analyzing on an LSRII flow cytometer (BD Biosciences). For cell sorting ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer (PBS with 2% Heat-inactivated FCS) and fixed in 1x BD cellFIX (BD Biosciences). Samples were acquired on the LSRFortessa (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... the cells washed and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb used in this study were obtained from BD Biosciences and Biolegend ...
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bioRxiv - Immunology 2020Quote: OT-I cells were expanded for 5 days in the presence of IL-2 after transduction and sorted on Thy-1.1 surface expression on a BD FACSAria Fusion cell sorter (BD Biosciences) directly into RLT Plus lysis buffer (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... at a concentration of 1 x 106 cells/mL with RPMI plus 2 μL/mL Leukocyte Activation Cocktail with GolgiPlug (BD) and incubated for 4 hours at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... cells were pelleted and then resuspended in 2 mL of 0.5% BSA in D-PBS for cytometer-based sorting (FACSAria IIIu cell sorter, BD Biosciences). An aliquot of 200 µL stained cells was saved as input for analysis ...
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bioRxiv - Immunology 2021Quote: ... followed by FcR blockage with mouse serum (2%) and FcR blocking anti-mouse CD16/32 antibodies (clone 2.4G2, BD Biosciences). Next ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice in PBS with 0.5% BSA and 2 mM EDTA and run on LSR II flow cytometer (BD Biosciences). Data were analyzed using FlowJo software ...
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bioRxiv - Bioengineering 2021Quote: ... All transformants were checked for the petite phenotype by growth on YEP-glycerol (1% w/v Bacto-yeast extract-BD Biosciences 212750, 2% w/v Bacto-peptone-BD Biosciences 211677 ...
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bioRxiv - Immunology 2021Quote: Cells resuspended in cold staining buffer (2% FCS in PBS) were blocked with mouse Fc Block (BD Biosciences, catalogue# 553141) for 10 minutes at 4°C and stained with a panel of antibodies (Supplementary Table 5) ...
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bioRxiv - Immunology 2021Quote: ... tibias and pelvis were harvested by crushing in PBS containing 2% of FBS and erythrocytes were lysed using a hypotonic buffer from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 concentration were measured on non-diluted or 10-fold diluted supernatants using Human cytometric assay from BD according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the invading cancer cells were fixed with 100% cold methanol and stained with 0.05 mg/mL 4,6-diamidino-2-phenylindole (DAPI; BD Biosciences, 564907). The invading cells in each chamber were counted under a fluorescence microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... single cell suspensions were made (1.5 x 104 cells per ml) in the appropriate medium supplemented with 2% Growth Factor Reduced Matrigel (GFRM; BD Biosciences). 150μl of this mix was plated per well in a 96 well ImageLock plate (Essen Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and the concentration of IL-2 in culture supernatants was determined by ELISA according to the manufacturer’s instructions (BD Biosciences).
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bioRxiv - Genetics 2021Quote: ... in 200 μl of 2% FBS/PBS (v:v) and calculated using DIVA before sorting on a FACSAria fusion III cytometer (BD Biosciences). In order to maintain the ratios of MuSCs ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5mM EDTA) containing the indicated fluorophor-conjugated antibodies (CD138-APC [281-2] and B220-PE [RA3-6B2] purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2020Quote: One hundred microliters of whole blood from each of the cynomolgus macaques was stained with combinations of fluorescence-conjugated monoclonal antibodies: anti-CD3 (clone SP34-2, Alexa700; BD), anti-CD4 (clone L200 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by fixation in 2% PFA in PBS before subjecting them to flow cytometry using the LSR-II (BD Biosciences). Data was analyzed by FlowJo v10 and graphed by Prism 8 (GraphPad).
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bioRxiv - Immunology 2020Quote: ... USA) for 2 hrs followed by the addition of Brefeldin A (10 µg/ml) (BD Biosciences, San Diego, CA, USA) after which the cells were further incubated for 16 hrs ...
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bioRxiv - Immunology 2020Quote: ... T cells were 2-way sorted from PBMC pre-gated on CD4+ CD14− viable lymphocytes using a FACS Aria (BD) and the antibodies listed in supplementary material procured from Biolegend or BD ...
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bioRxiv - Immunology 2021Quote: ... and I53-50B components of the NP-scaffold (1.2 µg/ml of each peptide) in the presence of 1 µg/ml of anti-CD28 (clone CD28.2, BD Biosciences) and anti-CD49d (clone 9F10 ...
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bioRxiv - Microbiology 2021Quote: ... were routinely grown at 37 °C under anaerobic conditions in a Bactron III-2 anaerobic chamber (Shellab, USA) in BHIS medium: 3.7% weight vol−1 brain heart infusion broth (BD, USA) supplemented with 0.5% weight vol−1 yeast extract (BD ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed 2 times with flow buffer and incubated with BD Cytoperm™ Permeabilization Buffer Plus (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... rat anti-mouse IL-12p40/p70 (10 μg/mL, clone C17.8), rat anti-mouse IL-2 (10 μg/mL, clone S4B6) (all from BD Biosciences), were included in the cultures ...
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bioRxiv - Neuroscience 2020Quote: ... or formalin solution (2% in 1x PBS) in the plantar surface of the right hind paw using a standard 28G insulin syringe (BD) and video recorded from below for either 15 or 60 minutes respectively ...
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bioRxiv - Cell Biology 2022Quote: ... cultured PAECs were incubated with 2’,7’–dichlorofluorescin diacetate (DCFDA; 1μM) for 10 minutes at 37°C and then assessed by flow cytometry (BD LSRFortessa).
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bioRxiv - Microbiology 2022Quote: ... Live cells were measured every 2-3 days on the BD FACS Celesta Flow Cytometer (BD Biosciences, Franklin Lakes, NJ) using a known amount of spike-in AccuCount Blank particles (Spherotech ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells then were trypsinized and resuspended in PBS containing 2% FBS and dTomato-positive cells were sorted using a BD FACSAria III flow cytometer (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... The constructs were then co-transformed into yeast AH109 strain and plated on SD selective media and no detectable self-activation was observed (SD -His-Leu, + 2 mM 3-AT or SD -His-Trp, + 40 mM 3-AT (for OsSHR1-BD) or 20 mM 3-AT (for OsSHR2-BD)) ...
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bioRxiv - Cell Biology 2024Quote: Twenty µl of platelet post activation supernatants were incubated with 2.5 µl of phycoerythrin (PE)-conjugated mouse anti-human CD62P (clone AK-2, BD Biosciences), 2.5 µl of PE-conjugated CD61 (clone VI-PL2 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were blocked for 30 minutes in FACS buffer (2% FBS 1X PBS) containing Fc Block anti-CD16/32 (2.4G2, BD Biosciences) (1:100 dilution) ...
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bioRxiv - Immunology 2024Quote: ... and then stained for 30 min at 4°C in PBA buffer (PBS, 0.5% BSA, 2 mM EDTA) in the presence of Fc Block (1:50 dilution, BD-Biosciences), using the following antibody combination ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cytokine staining was performed with 2 x 106 cells after 3.5h of in vitro stimulation in Opti-MEM media supplemented with Golgiplug (BD Biosciences), 10 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: The titles and abstracts of 2 341 articles retrieved from the preliminary search were screened and cross-checked by two independent reviewers (ZL and BD) and discrepancies were resolved through discussion with a third researcher (DM) ...
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bioRxiv - Cancer Biology 2023Quote: ... Immune cells were resuspended in surface staining mix containing FACS buffer with 1/2 of final volume of Brilliant Stain Buffer (BD), 50 μg/ml anti-mouse CD16/CD32 (2.4G2 ...