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Citations for Becton, Dickinson and Company :
1551 - 1591 of 1591 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... followed by washing with PBS and fixation/permeabilization using the BD Cytofix/Cytoperm kit according to the manufacturer’s protocol (BD Biosciences, San Jose, California). Cells were then pelleted and resuspended in the Click-iT OP-puro reaction cocktail with AlexaFluor 488 picolyl azide (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Intracellular staining was performed after fixation and permeabilisation of the cells using FOXP3 Fixation/Permeabilisation Solution kit (BD Biosciences, Frankin Lakes, NJ, USA) using manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... 250,000 splenocytes were plated per well to perform an IFN-gamma ELISPOT per protocol using a Mouse IFN-gamma ELISPOT Kit (BD Biosciences; cat. #551083). Each sample was plated in triplicate with peptide or without peptide as a negative control ...
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bioRxiv - Immunology 2023Quote: ... Analysis of intracellular antigens was done by using the same fixation and permeabilization as was described under “Metabolic labeling of nascent proteins” or using BD Cytofix/Cytoperm fixation/permeabilization kit (BD Biosciences, cat#554714). Maximum of 3 x 106 cells per reaction ...
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bioRxiv - Pathology 2023Quote: ... The culture medium was sampled by adding 1-3 drops to microscope slides followed by Gram stain (BD BBLTM Gram Stain Kits; B12539) and imaging.
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bioRxiv - Biochemistry 2024Quote: ... Antibody-stained cells were washed in FACS staining buffer and resuspended in 20 µL 7-actinomycin D (7-AAD, BD Pharmigen flow kit) for 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: Wild type RPE1 cells or RanGAP1ΔNES clones 2 and 10 were grown on 10 cm plates to 40-70% confluence and pulsed with 10 µM BrdU (BD Pharmigen flow kit) for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining for flow cytometry analyses was performed according to the BD Pharmingen BrdU Flow Kit protocol (557891, BD Biosciences, San Jose, CA, USA) as previously used42 Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 37°C and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Cancer Biology 2022Quote: ... The co-cultures were incubated for 6 hours at 37°C and 5% CO2 prior to cell staining using the Cytofix/Cytoperm Fixation Kit (BD Biosciences, Cat# 554715/555028).
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Physiology 2021Quote: ... islets were dispersed and dead cells were labeled using the LIVE/DEAD Fixable Aqua (405 nm) Dead Cell Stain Kit (BD Biosciences, San Jose, CA). EdU detection ...
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bioRxiv - Immunology 2021Quote: ... T2 and its derived cell lines were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences, Becton, Dickinson and Company, Franklin Lakes, NJ). Washed cells were then resuspended in 1x PermWash buffer (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... IL-2 and IFNγ cytokine concentrations were analyzed by sandwich ELISA according to manufacturer’s kit protocol (BD Opteia human IL-2 and human IFNγ). In vivo cerebellum lysates were analyzed for cytokines similarly ...
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bioRxiv - Immunology 2021Quote: ... followed by a cell viability stain (either Live/Dead Fixable Aqua Dead Cell Stain Kit, 405 nm excitation, Invitrogen ref. no. L34966; or Fixable Viability Stain 700; BD Horizon Cat. No. 564997). For tumor cell analysis ...
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bioRxiv - Immunology 2021Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlugTM, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Cell Biology 2022Quote: ... and their viability was tested using the annexin-PI apoptosis detection kit (MBL, USA) followed by flow cytometry analysis (BD Biosciences FACS Canto II).
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bioRxiv - Pathology 2021Quote: ... The cells were stained with fluorescein isothiocyanate (FITC)-conjugated annexin V and propidium iodide (PI) using the Annexin V-FITC & PI Apoptosis Detection Kit (, BD 货号 Biosciences, USA).
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bioRxiv - Immunology 2020Quote: We labeled each group of NALT cells separately with the Sample-Tags from the BD Mouse Immune Single-Cell Multiplexing Kit (BD Biosciences, San Jose, CA), described in “Single Cell Labeling with the BD Single-Cell Multiplexing Kits” protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Immunology 2021Quote: ... T cells were stained for surface antigens prior to fixation and subsequently processed for intracellular cytokine staining following BD Cytofix/Cytoperm™ kit (BD Biosciences cat 554714) instructions.
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bioRxiv - Neuroscience 2021Quote: ... 20 μl of each sample was analysed in duplicates using the BD™ CBA Human Soluble Protein Master Kit (#556264; BD Biosciences, CA, USA) alongside the BD™ CBA Human Flex Sets for interleukin 1ß (IL-1ß ...
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bioRxiv - Physiology 2022Quote: ... Then final cDNA library was generated from double strand full length cDNA by random priming amplification with BD Rhapsody cDNA Kit (BD Biosciences, Cat. No. 633773) and BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: ... Following surface staining cells were fixed and permeabilized using a BD Cytofix/ Cytoperm Fixation/ Permeabilization Solution kit (Cat# 554714 BD Biosciences, Franklin Lakes NJ) followed by intracellular staining with anti-IFNγ ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Neuroscience 2024Quote: ... PCR amplification of the fragments and their subsequent ligation was performed using the In-Fusion cloning kit and online tools (BD Clontech, Takara Bio, USA), or they were synthesized by GenScript (Piscataway ...
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bioRxiv - Microbiology 2024Quote: Trophozoite apoptosis and reactive oxygen species (ROS) were assessed using a PE Annexin V apoptosis detection kit I (BD Biosciences, San Jose, CA, USA) and a ROS assay kit (Dojindo Molecular Technologies ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cell counts were determined by flow cytometry using the TruCount kit in a FACSCanto II instrument (BD Bioscience, San Jose, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... analysis of sub-G0/G1 DNA content as previously described (23) and by measurement of active caspase-3 by flow cytometry using a commercial kit (BD Biosciences, Franklin Lakes, NJ).
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bioRxiv - Immunology 2024Quote: ... Sample viability was confirmed using the fixable viability dye as per manufacturer recommendation (LIVE/DEAD Aqua Cell stain kit, Thermofischer Scientific) and measured using flow cytometry (BD LSR II, BD Biosciences). The median cell count of thawed PBMCs is (4 million cells per participant) ...
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bioRxiv - Immunology 2021Quote: ... or PMA + ionomycin in the presence of brefeldin A for 4 h and stained using the Cytofix/Cytoperm Kit (BD Bioscience, Franklin Lakes, NJ, USA). Foxp3-positive cells and apoptotic cells were detected using the BD Foxp3 Staining Kit (BD Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of the cells was measured post 48 hours of drug treatment using annexin-V-7AAD viability kit (BD Pharmingen, San Diego, CA, USA) as per the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Splenocytes taken from allo-transplanted recipients were stimulated with anti-CD3/anti-CD28 (2.5ug/ml) for 6 hours in the presence of Golgiplug (BD Cytofix/Cytoperm Plus kit cat#555028) (1:1000) ...
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bioRxiv - Microbiology 2022Quote: ... PM-WCPV-S and PM-CVHSA-S respectively) in the presence of Brefeldin A (0.65 µL/mL, GolgiPlug™, BD Cytofix/Cytoperm Kit, Cat. 555028), co-stimulatory antibodies anti-CD28 (BD Biosciences Cat ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 and IL-10 levels in cell culture supernatants were determined using the OptEIA™ mouse IL-10 ELISA kit (BD Biosciences, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and tumour necrosis factor α concentrations were quantified in lung homogenates using an ELISA kit (BD Opt EIA Mouse IL-6 ELISA Sets, #555240; BD Biosciences Franklin Lakes, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... were treated with DNAse (150μg/ml) and then incubated with an anti-BrdU antibody (APC BrdU Flow kit, BD; dilution 1:100 in 1X Perm/Wash buffer) for 1 hour in ice.
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bioRxiv - Immunology 2024Quote: IL-17A and IL-10 in supernatant were quantified simultaneously using a BD™ Mouse Cytokine Cytometric Bead Array (CBA) kit and BD™ CBA FCAP array software (BD Biosciences, San Jose, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: DST for first line anti-tuberculosis drugs was performed on the BD BACTEC™ MGIT™ 960 automated mycobacterial detection system with the BD BBL™ MGIT™ AST SIRE kit (Becton, Dickinson and Company, NJ). All isolates were tested at the following critical concentrations as described by the manufacturer and the Clinical and Laboratory Standards Institute (CLSI ...