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Citations for Becton, Dickinson and Company :
1401 - 1450 of 1591 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... GMPGFP+ and GMPGFP- or clonal HoxA9 cells were sorted directly in the fixation buffer from the Fixation/Permeabilization kit (BD Biosciences). Cells were then washed with PBS supplemented with 3% BSA followed by the Click-iT reaction as described above ...
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bioRxiv - Cell Biology 2020Quote: ... intracellular staining with anti-FoxP3-FITC antibodies (clone: FJK-16s; Thermo Fischer Scientific) was performed with the BD Cytofix/Cytoperm Kit (BD Biosciences) according to the manufacture instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... treated with 75µM cisplatin and stained with Annexin V conjugated to Alexa Fluor 488 dye and propidium iodide (PI) using Dead Cell Apoptosis Kit (BD Biosciences) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... fixable viability dye was included with the surface staining antibodies (20 minutes, RT) and intracellular staining used the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... then cell culture supernatant were harvested and analysed using a BD Cytometric Bead Array (CBA) Human Soluble Protein Master Buffer Kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Concentrations of TNF-α and IFN-γ in sera on day 21 after allogeneic BMT were measured with specific anti-mouse ELISA kits from BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IFN-γ in the serum of experimental monkeys were detected with the Non-Human Primate (NHP) Th1/Th2 Cytokine Kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The enzyme activity was stopped with fetal bovine serum (FBS) followed by the fixation and staining with the APC BrDU Flow Kit – Part A (552598, BD Pharmingen) according to the manufacturer’s recommendation with some exceptions ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed after treatment with Fixation/Permeabilization solution and in the presence of Perm/Wash buffer (Cytofix/ Cytoperm kit, BD Biosciences) using anti-mouse IFN-γ FITC ...
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bioRxiv - Immunology 2022Quote: ... The Foxp3 staining kit was used for intracellular transcription factor staining and the Cytoperm/Cytofix Plus staining kit was used for cytokine staining (BD Biosciences). Data was analyzed using FlowJo (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with 500 μL permeabilization buffer (from kit described above) and stained with 150 μL permeabilization buffer containing 1:400 Ki67-Alexa-Fluor antibody (561126, BD Biosciences) for 30 minutes at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... and permeabilized using BD Cytofix/Cytoperm kit before incubation with BV605 anti-human IFN-gamma (B27 clone) and BUV395 anti-human TNF-alfa (MAB11 clone, BD Biosciences) in permeabilization buffer at 4°C for 30 min in the dark ...
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bioRxiv - Cancer Biology 2020Quote: Cells were double stained with propidium iodide (PI) and Annexin V by using FITC Annexin V apoptosis detection kit (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-4 and IL-10 were quantified using the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine kit (BD Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Total CD4+ T cells were isolated by negative magnetic selection using a CD4 T cell isolation kit (Stemcell) and CD4+CD25hiCD127lo-neg Treg cells were sorted on a FACS Aria (BD Biosciences). Treg cells were cultured in RPMI 1640 medium supplemented with 5% Human serum ...
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bioRxiv - Genetics 2021Quote: ... at least 2x106 iPSC-PPC cells were fixed and permeabilized using the Fixation/Permeabilization Solution Kit with BD GolgiStopTM (BD Biosciences) following manufacturer recommendations ...
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bioRxiv - Bioengineering 2021Quote: ... for assays which required non-transcription factor internal staining (cytokines alone) fixation and permeabilization was performed using the Cytofix/Cytoperm kit (BD Biosciences) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences, 1:100), -Sca-1 (E13-161.7 ...
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bioRxiv - Pathology 2021Quote: ... CD11b) and Sca1+/c-Kit+ cells in lineage negative cells determined via flow cytometry on a BD LSRII instrument (BD Biosciences). Data was analyzed on FlowJo software (Treestar) ...
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bioRxiv - Immunology 2021Quote: ... 4 and 6 hours and cytokines detected by intracellular cytokine staining (see Table S2 for antibodies list) using Fixation/Permeabilization Solution Kit (BD Biosciences), unstimulated cells were used as a control.
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bioRxiv - Immunology 2020Quote: ... stimulation of single cell suspensions in the presence of GolgiPlug for 4 hours prior to intracellular staining using BD Cytofix/Cytoperm kit (BD Biosciences). Viability was determined using Fixable Viability Dye eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... either cell supernatants or cell extracts were used for cytokine estimation with specific kits- TNFα (cat no: 88-7346-77, eBioscience) and IL1β (cat no: 557953, BD Biosciences) ELISA kits according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... Spleen B cells were analyzed for BrdU incorporation (by anti-BrdU mAb staining) and DNA contents (by 7–AAD staining after cells were permeabilized) using the APC BrdU Flow kit® (BD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and intracellular staining was performed after the fixation and permeabilization of the cells (intracellular staining kit from ThermoFisher or BD Biosciences). Analysis of cells collected from in vivo experiments were performed following Fc block (2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... We only used iTreg cells for experiments if samples achieved at least 80% Foxp3 positive cells (00552300, Foxp3 Staining Kit, BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... Spike -specific T cells were measured by surface staining followed by fixation and permeabilization using BD fixation/permeabilization solution kit (BD Biosciences,) and intracellular cytokine staining (table S1) ...
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bioRxiv - Immunology 2020Quote: Cytokine concentration in the wounds was determined by flow cytometry using the kit CBA Mouse Inflammation (BD Biosciences, San Diego, CA), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were harvested 48 hours after the stimulation and cytokines were quantitated by the BDTM CBA Mouse Th1/Th2 Cytokine kit (BD Biosciences) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated and immunolabeled with anti-CD4 and anti-Foxp3 antibodies and BrdU incorporation was analyzed by a BrdU Flow kit per the manufacturer’s protocol (BD Pharmingen, 552598) (15).
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bioRxiv - Neuroscience 2022Quote: ... were quantitatively determined in spinal cord homogenates by flow cytometry analysis using the mouse Cytometric Bead Array (CBA) Th1/Th2/Th17 kit and specific mouse Flex set systems (BD biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and tumor necrosis factor (TNF) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). Readings were done on an AttuneTM Nxt Flow Cytometer.
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bioRxiv - Immunology 2023Quote: ... After an additional 3 days BDC2.5 T cell proliferation was assessed by flow cytometry and supernatants were collected for analysis with the mouse Th1/Th2/Th17 Cytokine Kit (BD Biosciences) and FCAP Array Software v ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were further analyzed for TNFα and/or IFNγ production using respectively BD OptEIA Human TNFα and IFNγ ELISA kits (BD Biosciences). Flat-bottom 96-well plates (COSTAR ...
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bioRxiv - Cancer Biology 2023Quote: ... The staining procedures were performed by the use of a Cell Cycle and Apoptosis Analysis Kit (Yeasen) and the cell cycle were detected by flow cytometry analysis (BD FACSCanto).
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bioRxiv - Neuroscience 2023Quote: Cells were directly sorted into 1.5 ml tubes containing 100 µl of extraction buffer (XB, Arcturus PicoPure RNA isolation kit) with a FACSAria III SORP (BD Biosciences) flow cytometer equipped with a 90 µm-diameter nozzle ...
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bioRxiv - Cell Biology 2023Quote: ... MPP1-4 from WT and mutant mice were permeabilized and fixed according to the manufacturer’s instructions with the FOXP3 permeabilization kit (Foxp3/Transcription Factor Staining Buffer Set) and then labeled with Ki-67 Ab (clone B56, mouse IgG1; BD Biosciences) and DAPI before flow cytometric analysis ...
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bioRxiv - Immunology 2023Quote: ... Medium was exchanged after 48 h of incubation and after 72 h cells were washed and permeabilized/fixed using Cytofix/Cytoperm kit (BD Biosciences). Cells were stained with VL-4 hybridoma cell line supernatant containing LCMV NP-specific antibodies for 60 min at RT ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The distribution of cell populations throughout the cell cycle and the fraction of cells capable of incorporating BrdU were determined using the BrdU Flow Kit (BD Biosciences). After treatments cells were incubated with BrdU (10 ⎧M ...
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bioRxiv - Neuroscience 2022Quote: ... and tumor necrosis factor alpha (TNFα) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). A LEGENDplex™ HSC Myeloid Panel kit (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were recovered and the amounts of IL-6 were analyzed by cytokine-specific ELISA kits (BD Biosciences, Mountain View, CA).
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bioRxiv - Neuroscience 2024Quote: The rate of apoptosis induced by microRNA-29a-5p mimics and inhibitors transfection in astrocytes after OGD/R was determined using Annexin V/PI kit (BD Biosciences). Briefly ...
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bioRxiv - Genomics 2024Quote: ... at least 2 x 106 cells were fixed and permeabilized using the Fixation/Permeabilized Solution Kit with BD GolgiStop TM (BD Biosciences) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Cell apoptosis was measured by co-staining cells with Annexin V and 7-AAD using the PE Annexin V Apoptosis Detection Kit I (BD Biosciences), according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with ice-cold FACS buffer (DPBS supplemented with 2% FBS and 2 mM EDTA) and subjected to fixation and staining with Cell Fixation and Permeabilization Kits (BD Biosciences). Incubation with antibodies was performed for 1h at RT ...
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bioRxiv - Immunology 2024Quote: ... and CD45.2+ peripheral blood leukocytes were FACS-sorted and labeled using the BD Rhapsody Single-Cell Multiplexing CTT Kit (BD Biosciences, 626545). On average ...
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bioRxiv - Cell Biology 2023Quote: ... Post incubation cells were processed for apoptosis using flow cytometry according to the protocol described in FITC Annexin-V apoptosis detection kit (BD Pharmingen).
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bioRxiv - Genetics 2023Quote: ... 1.106 cells were incubated 20 min at 37°C in 100 μL solution BD cytofix/ cytoperm (BD PharmingenTM BrdU Flow Kit, 552598, BD Bioscience), washed with PBS and re-suspended in 700 µl of staining buffer (PBS with 3% SVF) ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... The final cDNA library was generated from double strand full length cDNA by random priming amplification with the BD Rhapsody cDNA Kit (633773, BD Biosciences) and the BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (633774 ...
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bioRxiv - Immunology 2023Quote: ... and either resuspended in PBS and directly analyzed or fixated using the BD Cytofix/Cytoperm fixation and permeabilization kit (BD Bioscience) according to the manufacturer’s protocol ...