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Citations for Becton, Dickinson and Company :
1501 - 1550 of 1691 citations for Recombinant Human CD80 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Flow cytometric analysis was performed by Green Fluorescent Protein (GFP) on FACSAria Flow Cytometer (BD Biosciences). FACS data was analyzed with FlowJo software (TreeStar Inc.).
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bioRxiv - Immunology 2021Quote: ... Protein transport inhibitor cocktail and Mouse TNF-α (560478) ELISA kit were procured from BD (USA). The following ELISA kits were brought from R&D ...
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bioRxiv - Immunology 2022Quote: ... Binding to P-selectin was measured by incubating with P-selectin IgG Fusion protein (BD Bioscience), washed and detected with fluorochrome conjugated secondary goat anti-human antibodies (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2023Quote: ... protein transport inhibitors 2μl/mL GolgiPlug (Brefeldin A) and 1.3μl/mL GolgiStop (Monensin) (BD, Biosciences, USA) were added to the tubes for 8 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... and stained with the antibodies against the following mouse proteins: SiglecF (clone E50-2440; BD Pharmingen), Ly6G (clone 1A8 ...
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bioRxiv - Neuroscience 2023Quote: ... protein transport inhibitors 2μl/mL GolgiPlug (Brefeldin A) and 1.3μl/mL GolgiStop (Monensin) (BD, Biosciences, USA) was added to tubes and further incubated for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... astrocytes (1×106) were incubated with protein transport inhibitor containing Brefeldin A (555029 Golgiplug, BD Biosciences) and Monensin (554724 Golgistop ...
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bioRxiv - Microbiology 2023Quote: ... The expression level of the surface S proteins was detected by FACS Canto II (BD Biosciences) and analyzed by FlowJo software v10.7.1 (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES [pH7.4]) with 25% high protein Matrigel (354248; BD Biosciences). PDX single cell suspensions were obtained by mechanically dissociated (12-141-363 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells expressing high levels of fluorescent proteins were sorted using flow cytometry (BD Influx cell sorter).
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bioRxiv - Cancer Biology 2021Quote: Cells from the cell line U3065 obtained from the Human Glioma Cell Culture (HGCC) resource [35] were suspended in stem cell medium and plated on 384 well plates (BD Falcon Optilux TC #353962) coated in laminin ...
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bioRxiv - Cancer Biology 2020Quote: ... Enumeration of human CD45+ cells in spleen and bone marrow was performed by flow cytometry after staining with BV605-conjugated human-specific CD45 antibody (clone HI30, BD Biosciences, San Jose, CA) using a BD LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions at 5 × 107 cells per ml were stained with a cocktail of fluorochrome conjugated human antibodies (BD Biosciences, San Jose, CA), anti-human IgG APC ...
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bioRxiv - Bioengineering 2020Quote: ... Coated beads were then incubated with anti-human CD63 (eBioscience, San Diego, CA) and analysed with a BD AccuriTM C6 (BD Biosciences, San Jose, CA).
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C with a cocktail of mouse anti-human antibodies: CD19 Alexa 700 (HIB19, BD Biosciences, San Jose, CA), CD21 BV421 (B-ly4 ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated at 1×106 cells/ml with 10 μg/mL soluble Leishmania antigen (SLA) plus 1 μg/mL purified NA/LE anti-human CD28 (clone CD28.2, BD Biosciences, San Jose, CA, USA) at 37°C / 5% CO2 for 15 h and subsequently incubated in the presence of brefeldin A (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Biochemistry 2020Quote: ... and TNF in the supernatants was determined by using Cytometric Bead Array (CBA) - Human Inflammatory Cytokines Kit (551811, BD Biosciences, San Jose, CA, USA), that allows the determination of the indicated human cytokines simultaneously ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole blood was collected from human volunteer donors with informed consent in 8 to 10 BD Vacutainer CPT 8 ml tubes (BD Biosciences, San Jose, CA). The blood samples were mixed immediately prior to centrifugation by gently inverting tubes 8-10 times ...
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bioRxiv - Immunology 2021Quote: ... harvested and stained for flow cytometry as described (37) using antibodies to human surface antigens: CD4-APCH7 (SK3, BD Biosciences Franklin Lakes, NJ, USA), CD8-PerCP (SK1 ...
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bioRxiv - Immunology 2023Quote: ... a total of 50 ml of whole human blood was collected by a trained phlebotomist from healthy donors in BD Vacutainer® EDTA tubes (BD Vacutainer®, USA). This was previously approved by the RVC’s Ethics and Welfare Committee (URN 2019 1916-3).
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bioRxiv - Molecular Biology 2023Quote: ... Evaluation of proliferation in tissues using antibodies against Ki67 or BrdU (anti-human Ki67 and anti- BrdU monoclonal antibodies; Thermo-Fisher Scientific and BD BioSciences, USA, respectively) was performed as previously described {GarciaMelendrez:GKcY05su } ...
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bioRxiv - Cancer Biology 2023Quote: Ba/F3 cells expressing human MPL protein that were either cultured normally or treated with drugs were harvested and stained for surface MPL using PE-conjugated anti-human MPL antibody (BD Pharmingen 562159,1:50 dilution) in staining buffer for 30 min at 4°C.
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bioRxiv - Immunology 2024Quote: ... blocked with 2% BSA in phosphate buffer pH=7.4 for 30min at RT and incubated with mouse anti-human LAMP1 (BD Biosciences, Franklin Lakes, NJ, USA) for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: 25 μL protein G Dynabeads were coated with 2 μg of anti-BrdU antibody (#555627, BD Biosciences) for 10 min at room temperature and then blocked for 30 min at room temperature in blocking buffer (0.1% PVP ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for detection of the respective proteins: mouse α-Cdc27 (BD Biosciences, #610455), mouse α-cyclin B2 (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™) for 12 hours ...
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bioRxiv - Immunology 2020Quote: ... followed by fluorochrome-conjugated antibodies for cell surface proteins before fixation and permeabilization (BD Cytofix/Cytoperm Kit). Antibody staining for intracellular cytokines was then performed in permeabilization buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1.5 mg of protein was immunoprecipitated with 2 µg of SRPK1 or SRPK2 antibodies (BD Biosciences). Immunoprecipitated containing beads were then washed with lysis buffer supplemented with 500 mM NaCl and half sample was resuspended in loading buffer ...
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bioRxiv - Immunology 2022Quote: ... and levels of protein expression were analyzed by flow cytometry using a FACS Canto II (BD Biosciences) followed by analysis using FlowJo software (Tree Star).
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bioRxiv - Plant Biology 2020Quote: ... Yeast cells co-expressing proteins fused to the Gal4 activation domain (AD) and DNA binding domain (BD) were selected on medium lacking the amino acids leucine (L ...
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bioRxiv - Immunology 2021Quote: ... Intracellular cytokine staining was performed for macrophage inflammatory protein 1β (MIP-1β; D21-1351, FITC, BD Biosciences) interferon-γ (IFN-γ ...
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bioRxiv - Molecular Biology 2021Quote: ... 750 μgs of protein were incubated with 25 μL of TALON Metal Affinity Bead suspension (BD Biosciences) in a 500 μL final volume for 30 min at 4°C with slow rotation ...
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bioRxiv - Immunology 2020Quote: ... cells were stained for surface proteins if required and then fixed in Cytofix fixation buffer (BD Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% penicillin–streptomycin and 10 mM HEPES (pH 7.4) with 25% high-protein Matrigel (354248; BD Biosciences)).
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bioRxiv - Genetics 2019Quote: ... Constructs encoding full-length or fragments of bait proteins fused to a DNA binding domain (GAL4-BD) were used to screen human oligo-dT primed and bovine random hexamer primed retinal cDNA libraries ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
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bioRxiv - Immunology 2022Quote: ... The following mAbs were used: anti-CD3ε peridinin chlorophyll protein (PerCP)-Cy5.5 (clone 145-2C11, BD Biosciences), anti-CD8α BUV805 (clone 53-6.7 ...
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bioRxiv - Physiology 2023Quote: ... livers protein lysates were incubated with fluorescent substrate Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) at 37°C and the reaction was monitored for at least 4 h to track changes in fluorescence (λ excitation of 390 nm and λ emission of 510 nm) ...
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bioRxiv - Microbiology 2023Quote: ... leading to a fusion protein with a GAL4 activation domain (AD) or GAL4 DNA-binding domain (BD) either at the N-or C-terminus ...
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bioRxiv - Immunology 2023Quote: ... protein transport inhibitor was prepared according to the manufacturer’s guidelines (RRID:AB_2869014, BD Science, San Jose, CA, USA) and added to all samples ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then stained for 30min at 4°C with the following antibodies: monoclonal anti-human CD33 V450 (WM53; BD Biosciences, San Jose, CA, USA); HLA-ABC FITC (W6/32 ...
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bioRxiv - Immunology 2019Quote: Cytokine levels in clinical samples were assessed using the following commercially available kits: i) human Th1/Th2/Th17 Cytokine Kit (BD Bioscience, San Jose, CA, USA) based on the principle of cytometric bead array (CBA ...
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bioRxiv - Biochemistry 2020Quote: ... an affinity-purified mouse mAb that recognizes an epitope in the C-terminal NAD binding and catalytic domain of human PARP1 (clone 7D3-6, BD Biosciences #556493; 1:500 dilution); anti-cc-PARP1 ...
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bioRxiv - Immunology 2020Quote: ... for macrophages and pDC’s were identified by co-staining of PE conjugated mouse anti-human CD123 (BD Biosciences, USA, 1:20, 37°C for 2h) and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the cell suspensions purity was higher than 90% as assessed by fluorescent staining with PhycoErytherin (PE)-anti-human CD14 antibody (BD Biosciences, San Diego, CA, USA) using a Floid Cell Imaging Station (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... IgA and IgM antibodies were quantified in the CVL specimens using the human immunoglobulin cytokine bead array (CBA) flex set assay (BD Biosciences, San Jose, CA, USA). The Ig CBA assay was performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The following mouse and human monoclonal antibodies were utilized for virion capture assays: anti-PSGL-1 and anti-mouse IgG (BD Biosciences, Cat# 556053 and 557273) and anti-gp120s (clones 2G12 and PG9 acquired from the NIH ARP ...
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bioRxiv - Cancer Biology 2023Quote: ... to isolate GFP+ and GFP-populations from primary tumors after DAPI staining for live cells and from mice metastatic organs after labeling with human CD298-PE (BD, Cat#749741, clone P-3E10) and DAPI for human melanoma and live cells ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed twice with PBS as described above and the cell pellet was resuspended into 25 µl of FACS buffer (1% FBS in PBS) containing human FC block (BD Bioscience, # 564219; 1:200 dilution) and either 200 nM MLi-2 (synthesised by Natalia Shpiro ...