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Citations for Becton, Dickinson and Company :
1151 - 1200 of 1691 citations for Recombinant Human CD80 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: To measure secreted cytokine and chemokine levels a human IL-1β enzyme linked immunosorbent assay (ELISA) kit (BD, #557953), human IL-6 ELISA kit (R&D Systems ...
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bioRxiv - Microbiology 2019Quote: ... 0.02% NaN3 in PBS) and stained with a FITC-conjugated anti-human HLA-DR mAb (Clone Tü36; BD Biosciences). This antibody specifically binds to a monomorphic epitope of the HLA-DR αβ complex and not the isolated α or β chains or the HLA-DP and -DQ isotypes41 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcγ receptor; BD Biosciences) before staining for V450-human CD45 (BD Biosciences # 560368) ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated in PBS-0.5% BSA containing mouse anti-human Ki-67 (Alexa Fluor 647, clone B56, #558615, BD Pharmingen) for 30 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The cells were resuspended in FACS buffer (HBSS/ 1% FBS) then treated with Fc block (anti-mouse clone 2.4G2 and anti-human clone 3G8) purchased from BD and stained with specific antibodies ...
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bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
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bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatant was collected after incubation and assayed for cytokines using the human Th1/Th2/Th17 CBA kit (BD # 560484). IL-15 superagonist was quantified using the human IL-15/IL-15R alpha complex DuoSet ELISA (R&D Systems DY6924) ...
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bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
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bioRxiv - Cell Biology 2021Quote: ... and the following anti-human antibodies were used for surface staining: CD3-BV786 (clone SP34-2, catalog 563800, BD), CD56-BV605 (clone HCD56 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells from mouse tissues were incubated with nonspecific binding blocker (anti-mouse/human CD16/CD32 Fcy receptor, BD Biosciences) before staining for APC-human CD33 (Biolegend #303408) ...
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bioRxiv - Cancer Biology 2020Quote: ... 3×103 cells were resuspended in 30 μl PBS + 0.1% BSA and incubated with APC mouse anti-human CD44 (cat.no 559942, BD Bioscience) and FITC mouse anti-human CD24 (cat.no 555427 ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells (PBMCs) and plasma were isolated from human subject blood samples using CPT tubes (BD, 362753), and PBMCs were frozen in the liquid nitrogen vapor phase until further use ...
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bioRxiv - Microbiology 2022Quote: ... 1-4 days old CH1-2hSaW41 neonates were injected into the liver with 10-30 000 human cord blood CD34+ cells in 30μl of phosphate buffered saline (PBS) using a 30-gauge needle (BD) as previously described ...
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bioRxiv - Cancer Biology 2022Quote: ... Experiments involving isolation of human haematopoietic stem and progenitor cells (HSPCs) included single colour stained controls (CompBeads, BD Biosciences) and Fluorescence Minus One controls (FMOs) ...
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bioRxiv - Cell Biology 2020Quote: Human iPSCs were cultured in mTeSR®1 medium (STEMCELL) on growth factor-reduced Matrigel™ Matrix (BD Biosciences)-coated plates at 37°C in a 5% CO2 incubator ...
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bioRxiv - Neuroscience 2021Quote: ... which is an abundant splice isoform in human brain26 was engineered in the mammalian expression vector pIRES2-EGFP (BD Biosciences-Clontech ...
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bioRxiv - Systems Biology 2022Quote: ... 1 × 10^6 Cells were stained with PE anti-human CD11b antibodies (20 μL per test, BD Pharmingen, USA) and APC-Cy7 anti-human CD14 antibodies (5 μL per test ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in PBS 0.1% BSA 0.01% NaN3 containing the monoclonal antibody PE mouse anti-human CD54 (BD Pharmingen) or its isotype control at a concentration of 1/500 ...
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bioRxiv - Biochemistry 2019Quote: ... IL-6 levels in the supernatant were quantified using respective human enzyme-linked immunosorbent assay (ELISA) kits (BD OptEIA) as per the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... Bone marrow (BM) was collected 16 weeks post-transplantation and stained with human CD45-PE (clone HI30, BD Pharmingen). Animals were housed and handled in accordance with the guidelines set by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources ...
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bioRxiv - Immunology 2019Quote: Concentrations of human IL-1β in culture supernatants were measured by ELISA kit (BD Biosciences, San Jose, CA, USA). The mouse IL-1β ELISA Kit was purchased from R&D.
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bioRxiv - Cancer Biology 2019Quote: ... cells were blocked with anti-human FcR block (MACS miltenyi Biotec) and anti-mouse CD16/CD32 block (BD Biosciences). For cell sorting the MoFlo XDP or MoFLo Astrios (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: Murine organs and human LNs (1 cm3 or smaller in size) were fixed with BD CytoFix/CytoPerm (BD Biosciences) diluted in PBS (1:4 ...
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bioRxiv - Immunology 2020Quote: Fluorophore or biotin-conjugated antibodies with specificity to human cell surface antigens and cytokines were purchased mainly from BD Biosciences or Thermo Fisher Scientific and in some occasions from other suppliers as indicated below ...
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bioRxiv - Immunology 2020Quote: ... Cells were seeded (30,000-50,000/wells) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Pathology 2021Quote: ... and the resulting single-cell suspension was labeled with Phycoerythrin (PE)-conjugated mouse anti-human CD146 antibody (BD Biosciences) or CD31 antibody (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability and purity of the neutrophils were determined by anti-human CD66b PE (clone G10F5; BD Biosciences, 561650) and anti-human CD16 FITC (clone 3G8 ...
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bioRxiv - Genomics 2021Quote: Roughly 80 ml of human blood was drawn to and kept in spray-coated EDTA tubes (BD Vacutainer #366643) at 4°C ...
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bioRxiv - Genomics 2021Quote: Flow cytometry of iPSC-derived MSCs was performed using the BD Biosciences Human MSC Analysis Kit (BD Biosciences 562245), in combination with the Zombie Violet Fixable Viability Kit (BioLegend 423113) ...
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bioRxiv - Cancer Biology 2021Quote: ... dissociated tumor cells were Fc receptor blocked by incubating 1*106 cells/50-100 µl of staining buffer with 2.5 µg of Human Fc Block (Cat # 564220; BD) for 10 minutes.
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in staining buffer containing: 1:100 dilution V450 mouse anti-human CD31 (BD Biosciences, Mississauga, ON, Canada), 1:10 dilution PE mouse anti-human CD34 (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with PE-conjugated mouse monoclonal anti-human IGF-1R antibody (BD Biosciences, Franklin Lakes, NJ) or PE-conjugated isotype-matched control mouse antibody (IgG1k ...
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bioRxiv - Microbiology 2022Quote: ... human serum was isolated from venous blood of the same donors taken into serum separation blood collection tubes (BD Vacutainer ...
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bioRxiv - Cancer Biology 2023Quote: ... to block MHC-I and 30 μg/mL mouse anti-human HLA-DR/DP/DQ (clone Tu39, BD Biosciences) to block MHC-II for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Th2 cells were re-activated using 1μg/mL αCD3 (Purified NA/LE Mouse anti-Human CD3, 555329, BD Pharmingen) and 1μg/mL αCD28 (Purified NA/LE Mouse anti-Human CD28 ...
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bioRxiv - Neuroscience 2022Quote: iPSC lines were cultured on plates coated with human-qualified Matrigel (BD Biosciences; dilution 1:40 in DMEM-F12) in mTeSR (StemCell Technologies ...
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bioRxiv - Biophysics 2024Quote: ... Interferon gamma production was assessed by intracellular staining using mouse anti-human IFN-g FITC (BD Bioscience, clone 25723.11) and BD Cytofix/Cytoperm (BD Bioscience ...
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bioRxiv - Genomics 2024Quote: Human induced pluripotent stem cells (iPSCs) were maintained in mTeSR medium on plates pre-treated with Matrigel from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded (30,000-50,000/well) into wells pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) along with soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1:250 mouse anti-human CD15 clone HI98 [anti-Lewis x] (BD Biosciences, catalog no. 555400BD) or mouse-anti-human blood group Lewis a clone 7LE (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the following antibodies: anti-human CD45-APC (clone HI30, #555485, BD Bioscience, Franklin Lakes, NJ, USA) (hCD45 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cryopreserved human hepatocytes (Lot BD195: 2-year-old, Hispanic female, 1 × 106 hepatocytes, BD Biosciences, Ann Arbor, MI, USA) were transplanted under anesthesia into 2-to 4-week-old hemizygous cDNA-uPA/SCID mice through the spleen ...
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bioRxiv - Immunology 2023Quote: ... 100,000 PBMC per well were seeded in complete media (Advanced RPMI with 10% human serum and 1% L-Glutamine) supplemented with 1µg/mL of anti-CD28 antibody (Clone #37.51, BD Biosciences). The L598 compound was added to the cultures every two days at 2 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against human CD49e/Integrin α5 Chain (1:1000 for western blotting; BD Pharmingen; catalogue number 555614); mouse monoclonal antibody against human Integrin alpha-5 (1:100 for immunofluorescence ...
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bioRxiv - Biophysics 2023Quote: Monoclonal mouse anti-human CD44 (Clone G44-26) and monoclonal mouse anti-CD209 (Clone DCN46) were obtained from BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... GPIIIa, Luc.H11-FITC, #M031-1 or Integrin ⍺ 5/CD49e, Tap.A12-FITC and anti-human CD61-PE, #555754, BD Biosciences) and incubated for 15 min at RT in the dark ...
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bioRxiv - Biophysics 2024Quote: ... to a final concentration of 0.4 mg ml-1 for DHFR or 0.1 mg ml-1 for human fibronectin (BD Biosciences), with a total volume of 100 µl in a 96-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... the levels of secreted cytokines were quantified using the BD OptEIA™ Human IL-6 ELISA kit (BD Biosciences). Cells were trypsinized and counted for normalization of cytokine levels per cell.