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Citations for Becton, Dickinson and Company :
1501 - 1550 of 8232 citations for Goat Anti Human IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The membranes were then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk for 2 h ...
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bioRxiv - Immunology 2022Quote: ... and then incubated with human mAbs (at a concentration of 1 µg/ml) and mouse anti-Hisx6 antibody (1 µg/ml, BD Biosciences) in PBS-T 5% dry milk in each channel for 2 h ...
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bioRxiv - Immunology 2022Quote: ... whereas 100 µl PBS+/+ containing 20 ng/ml recombinant IL-1β supplemented with 100 µg/ml PTX3 and/or 100 µg/ml mouse anti-human CD62P (clone AK-4, BD Bioscience) was added to the apical side (upper chamber) ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was aspirated and the cell pellet was resuspended in 200uL of FACS buffer (PBS, 0.5% BSA, 2mM EDTA, 20mM Glucose) with 1:50 PE Rat Anti-Human CD49f antibody (BD Biosciences; 555736) and incubated on ice for 20 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells with SARS-CoV-2 peptides and positive control were co-stimulated with CD28/CD49d at a final concentration of 5 μg/ml (anti-human CD28/CD49d Purified Clone L293 L25 BD Biosciences). Basal IFN-γ production by PBMCs was included as background control in the absence of stimulation and co-stimulation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... activation of T-cells was measured by flow cytometry using the anti-human CD4 (FITC)/CD69 (PE)/CD3 (PerCP) antibody staining solution (BD Biosciences) in combination with anti CD25 (APC ...
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bioRxiv - Pathology 2021Quote: Cells were fixed in 2% PFA for 15 minutes at room temperature and incubated with mouse anti-human VE-Cadherin (BD Sciences) at 4°C overnight as previously described 36 ...
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bioRxiv - Immunology 2021Quote: Cells were co-cultured in R10 medium alone or in presence of K562 cells for 6 hours in the presence of anti-human CD107a (H4A3, FITC, BD Biosciences). Golgi plug and Golgi stop (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or with no target cells (or an E:T of 1:0) and cultured overnight before being staining with allophycocyanin (APC)-conjugated anti human-CD69 antibody (BD Biosciences #555533). Flow cytometry was performed using a BD- Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Immunology 2021Quote: ... To this end 1×104 targets per well and 2×104 effector cells per well were each preincubated for 2 h in 10 μg/ml anti-human CD178 antibodies (NOK-1 and NOK-2, BD Biosciences) or 5 μg/ml anti-human TRAIL antibody (RIK-2 ...
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bioRxiv - Genomics 2020Quote: Immune cells for bulk mRNA sequencing were incubated with Fc Block for 20 minutes and then stained with one of six panels of directly conjugated antibodies for 30 minutes: anti-human CD16 (BD 558122), CD123 (BD 560826) ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IFN-γ ELISA set (Cat no. – 555138) and anti-human IL-10 ELISA set (Cat no. – 555157) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Immunology 2022Quote: ... Human CD34+ stem cell purity was monitored by anti-human CD34 FITC (clone:581) and 7-AAD viability dye using LSR Fortessa X-20 flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2022Quote: FACS-sorted Tregs were plated at 50,000 cells/well in round bottom 96 well plates pre-coated with anti-human CD3 (2 μg/ml, clone UCHT1, BD Biosciences) and soluble anti-human CD28 (1 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... PECAM was stained 1:200 with the live labeling antibody Alexa Fluor® 647 Mouse Anti-Human CD31 (BD Pharmingen™), 2 min prior to imaging ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently hLSECs were purified via FACS by labelling with mouse anti-human CD31-FITC conjugated antibody (clone WM-59, BD Biosciences). Two independent preparations of transplanted iEC were used for single cell RNAseq ...
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bioRxiv - Immunology 2023Quote: ... BM aspirate samples from heathy donors using a Ficoll density gradient and stained with the following anti-human antibodies: IgD-Brilliant Violet 480 (Cat. No. 566138; BD Biosciences), CD3-BUV737 (Cat ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for 20 min a 4°C with the appropriate combinations of fluorochrome-conjugated anti-human antibodies for the identification of CD8 T cells and sub-populations: CD3 BV480 (clone UCHT1, BD Biosciences), CD8 BV421 (clone RPA-T8 ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: mouse anti-human CD3 conjugated to BUV737 (clone UCHT1, BD, 612750), mouse anti-human CD45 conjugated to FITC (clone HI30 ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed twice in PBS + 2% FBS and stained with mouse anti-human CD45 Pe-Cy5 (BD Bioscience, clone HI30) and mouse anti-human CD107a Alexa Fluor 647 for 30 minutes at 4°C in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... for IgG classes or IgE (BD Pharmingen, #553413) or with Alternaria extract (0.1 μg/well) ...
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bioRxiv - Immunology 2022Quote: ... IgG (BV786, BD Horizon, #564230, Clone G18-145) and IgM (PE ...
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bioRxiv - Neuroscience 2020Quote: ... Mac-3 (rat IgG, BD Biosciences; RRID: AB_394780), Iba1 (rabbit ...
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bioRxiv - Immunology 2022Quote: ... IgG Alexa 700 (clone G18-145, BD Biosciences), CD3 APC-Cy7 (clone SP34-2 ...
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bioRxiv - Immunology 2022Quote: ... IgG PE-Cy5 (clone G18-145; BD Biosciences), CD20 BV570 (clone 2H7 ...
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bioRxiv - Cancer Biology 2019Quote: ... or Alexa-Fluor 647 IgG κ isotype (BD Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... IgG Alexa 700 (clone G18-145, BD Biosciences), CD3 APC-Cy7 (clone SP34.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... using IgG beads (BD Biosciences, catalog number:552843) as negative control.
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bioRxiv - Immunology 2021Quote: ... IgG Alexa 700 (clone G18-145, BD Biosciences), CD3 APC-Cy7 (clone SP34.2 ...
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bioRxiv - Genetics 2022Quote: ... IgG (clone G18-145) (BD Biosciences, cat#: 561296), and IgM (clone G20-127 ...
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bioRxiv - Immunology 2023Quote: ... IgG Alexa 700 (clone G18-145, BD Biosciences), CD3 APC-Cy7 (clone SP34-2 ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... and rabbit IgG isotype (BD Biosciences, Cat.# 550875) were used ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Cancer Biology 2022Quote: ... were blocked for overnight in 5% goat serum 0.3% Triton in PBS and incubated with 10 µL/mL Alexa 647-conjugated anti-BrdU antibody (BD biosciences, 560209) or anti-cleaved Caspase 3 (Cell Signaling ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were then consecutively stained with FCRLs monoclonal rat antibody (Butovsky et al., 2014) (30 min), goat anti-rat APC antibody (Biogegend, # 405407) (20 min) and Cd11b PeCy7 antibody (BD, #553142) (20 min ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were washed with PBS-T and incubated with the Alexa-fluor 647 cleaved PARP primary and Alexa-fluor 488 goat anti-rabbit secondary antibodies (1:500 dilution, BD Bioscience) overnight at room temperature ...
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bioRxiv - Immunology 2023Quote: ... solution for 20 minutes followed by 20 minutes of antibody cross linking on ice with 2 µg/mL goat-anti mouse solution (BD, #53998). T cells were then activated via an incubation period at 37 °C in FACS buffer (PBS with 2% FBS and 2 mM EDTA ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated overnight with hamster anti-CD3ε antibody diluted in 1% goat serum/0.3% Triton-X100/TBS (BD Biosciences, 550277). The next day samples were washed in TBS and incubated at room temperature in goat anti-hamster 647 (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... the individual antibodies used for staining were mixed with one drop of negative control beads and anti-mouse IgG beads (BD Biosciences, CA, USA). For the dead cell stain ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then blocked with CD16/CD32 (1:500; Fc Block™; BD Biosciences, RRID: AB_394656) for 5 min and incubated for 30 min with optimal concentrations of fluorochrome-conjugated antibodies (Table S2 ...
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bioRxiv - Cancer Biology 2019Quote: Single cell suspensions were incubated on ice with mouse Fc receptor block (BD Biosciences, 1:200) followed by antigen-specific antibodies in FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with Fc block (1:500 dilution, Cat. # 553142, clone 2.4G2, BD Biosciences) diluted in FACS buffer for 5 minutes to block nonspecific binding of antibodies to cellular Fc receptors ...