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Citations for Becton, Dickinson and Company :
1501 - 1550 of 7150 citations for 6 Bromo 1 2 cyanoethyl 2 4 diethylamino phenyl benz cd indolium chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) plus 1 µg/ml ionomycin for 4 h at 37°C in the presence of GolgiPlug/GolgiStop (BD Pharmigen). Cells were stained for surface molecules as described above ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2×106 cells were incubated for 15 min at +4°C with blocking buffer (1xPBS, 1% BSA, 2mM EDTA) containing 5mg/ml α-CD16/CD32 (2.4G2, BD Biosciences). For surface staining ...
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Approximately 0.5-1 × 106 cells were stained for 15-30 minutes at 4°C in 96-well U-bottom plates (BD Biosciences) with directly conjugated antibodies (Table S8) ...
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bioRxiv - Cell Biology 2022Quote: Freshly dissected LW whole mount preparations from 6 wild-type and 4 Lrp2-/267 mice were incubated with rat anti-CD24 conjugated with Phycoerythrin (PE; 1:100; BD Pharmingen) in Neurobasal medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... cultured splenocytes were resuspended in 40 μL FACS buffer containing fluorochrome-conjugated antibodies and incubated 1 hr at 4 °C followed by cell fixation using Cytofix/Cytoperm buffer (BD Bioscience) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD8 APC (1:100, catalog no. 553035; BD Biosciences); rat anti-CD45 PE (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation was overnight at 4 degrees C with the following antibodies at 1:100 dilution in PBS + 3% BSA: CD24-FITC (clone ML5, BD Bioscience), CD271-APC (clone C40-1457 ...
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bioRxiv - Genetics 2023Quote: ... and CD43-B cells were cultured at a density of 5×105 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). Cultured cells were maintained daily at a density of 0.5-1×106 cells per ml ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated at 4°C for 30 min with APC-conjugated rat anti-mouse CD31 (1:200, BD Pharmigen, 551262). DAPI (5 mg/ml ...
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bioRxiv - Immunology 2023Quote: 50 × 103 effector cells were cultured alone or together with 5 × 103 tumour cells for 4 h at a 10:1 ratio in the presence of anti-CD107a-PE mAb (clone: H4A3; BD Biosciences) and monensin (3 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed two times with 150 µL PBS (centrifuged 5 min at 400 × g at 4 °C) and resuspended in 50 µL Fc-block (1:50, Purified Rat Anti-Mouse CD16/CD32, BD, #553142) diluted in FACS buffer (1x PBS/2% FBS/2mM ...
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bioRxiv - Cell Biology 2023Quote: ... E8.5 embryos were fixed with 4% paraformaldehyde (PFA) in PBS at 4°C for 3 h and incubated with FITC-conjugated anti-EEA1 (1:100, BD Bioscience) or Cy3-labeled anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2023Quote: Both WT and CD13KO mice of both genders were intraperitoneally injected with 1 mL 4% Brewers Thioglycollate (TG) solution (BD; 211716). After 48 hours mice were then euthanized via CO2 and cervical dislocation and injected with 10 mL cold PBS into the peritoneal cavity to collect peritoneal macrophages ...
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bioRxiv - Immunology 2023Quote: ... Effector cells were incubated with K562 and Nalm6 variants for 4 hours at a ratio of 5:1 with addition of Brefeldin A (GolgiPlug, BD Biosciences). Next ...
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bioRxiv - Bioengineering 2023Quote: ... The cell suspension was incubated with the following antibodies for 30 minutes at 4 °C: CD106-PE (VCAM-1, 555647, BD Biosciences), CD62E-FITC (E-Selectin ...
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bioRxiv - Cell Biology 2020Quote: ... FITC-conjugated Lineage cocktail 4 (562722, BD, clones RPA-2.10 ...
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bioRxiv - Cell Biology 2021Quote: ... A 4-laser LSR Fortessa (BD Biosciences) flow cytometer was used to collect data ...
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bioRxiv - Immunology 2020Quote: ... CD4 FITC (BD Pharmingen, clone RM4-4); CD8a PE (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... IL-4 (11B11) (both from BD Biosciences); T-bet (4B10 ...
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bioRxiv - Immunology 2020Quote: ... at 4°C: hCD45-BUV805 (HI30, BD), hCD3-PECyn7 (SK7 ...
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bioRxiv - Microbiology 2023Quote: ... and IL-4 (clone 11B11, BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... and media was tested by ELISA according to the manufacturer’s instructions (BD OptEIA Human IL-10 ELISA set #3555157 and BD OptEIA Human IL-1B ELISA set 2 #557953).
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bioRxiv - Cancer Biology 2019Quote: ... for 6 hours at 37 °C in the presence of Golgistop (monensin, BD) or Golgiplug (brefeldin ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated in 6-well plates (BD Falcon®, BD, ON, Canada). Upon treatments ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated in 6-well plates (BD Falcon®, BD, ON, Canada). Upon treatments ...
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bioRxiv - Pathology 2022Quote: ... Samples were acquired on an LSR II cytometer running FACSDiva 6 software (BD). Data were analyzed with FlowJo 10 (Tree Star).
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bioRxiv - Cell Biology 2021Quote: ... and grown in 6-well plates coated with collagen type IV (BD Bioscience). This protocol typically produces primary endothelial cell cultures that are approximately 99% pure (determined by immunocytochemistry with an anti-platelet endothelial cellular adhesion molecule 1 [anti-PECAM-1] antibody ...
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bioRxiv - Immunology 2020Quote: ... ELISA to determine IL-6 was performed using an ELISA kit from BD biosciences according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Splenocytes were incubated with Trp2 peptide (10−6 M) and Golgiplug (BD Biosciences) for 4–6 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Detection antibodies conjugated with biotin were anti-mouse-IL-6 (554402, BD Pharmingen), anti-mouse TNF-α (13-7349-81 ...
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bioRxiv - Molecular Biology 2022Quote: ... coated with 6 μg growth factor reduced Matrigel (BD Biosciences, Bedford, MA, USA). MKN45 cells (1.5×105 ...
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bioRxiv - Genomics 2023Quote: ... 6 mM MgSO4 and used Bacto™ Agar (Becton, Dickinson and Company; 29).
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bioRxiv - Immunology 2023Quote: ... and incubated with 20μL of the MultitestTM 6-color TBNK reagent (BD Biosciences) containing CD3 ...
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bioRxiv - Immunology 2023Quote: ... 5% CO2 for 6 hours in the presence of Golgi Plug (BD Bioscience).
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bioRxiv - Immunology 2024Quote: ... and incubated with 20µL of the MultitestTM 6-color TBNK reagent (BD Biosciences) containing CD3 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were fixed in 4% paraformaldehyde for 45 minutes at 4 °C then washed with 1xPerm/Wash (BD, 554723). For intracellular staining in tumor and spleen samples ...
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bioRxiv - Immunology 2021Quote: For TCR stimulation naïve and primed CD8+ T cells were incubated for 20 min at 4°C with biotinylated anti-CD3ε mAb (5 and 1 μg/mL, BD Biosciences #553060). Cells were washed and stimulated for the indicated time by adding streptavidin (20 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... The resulting solutions were diluted (10-1 - 10-4) and 10 µl were seeded in plates containing potato dextrose agar (PDA) (BD-Difco®) and Sabouraud agar (SBA ...
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bioRxiv - Synthetic Biology 2023Quote: ... A third of each cell sample was stained for 30 mins at 4°C with 1:100-dilutions of APC-conjugated anti-C-Kit/CD117 (BD Biosciences; 550412) and PE-Cy7-conjugated anti-CXCR-4/CD184 (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... labelled with cell hashing antibodies and isolated by FACS (all steps at 4°C): Cell suspensions were incubated with Fc-block (1:50, BD Pharmingen 553142) for 10min and subsequently incubated with mouse CD45 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: Trypsinized or MACS sorted cells were incubated at 4°C for 20 min with APC-conjugated anti-CD56 antibodies (1:40, BD Pharmingen #555518) or isotype control (1:40 ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: rabbit anti-AP-4 epsilon 1:400 for Western blots (612019; BD Transduction Labs); rabbit anti-ATG9A 1:200 for immunofluorescence and 1:1000 for Western blots (ab108338 ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with the following conjugated antibodies (1/100 dilution in PBS) for 15 minutes at 4°C: CD44 (PE, BD Biosciences 555479), EpCAM (FITC ...
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bioRxiv - Developmental Biology 2019Quote: ... containing 10 μM Y27632 (Wako) on 6-well plates coated with Matrigel (BD Biosciences). The cells were plated at 4.0 × 105 cells ...
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bioRxiv - Pathology 2019Quote: ... CD127 (HIL-7R-M21) and HLA-DR (G46-6) antibodies were purchased from BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Levels of human IL-6 were determined by enzyme-linked immunosorbent assays (BD Biosciences) as per the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... Myogenic differentiation was initiated 6 hrs after seeding cells onto Matrigel (BD Life Sciences) coated cell culture dishes (30,000 cells/cm2 ...
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bioRxiv - Immunology 2020Quote: HyIL-6 (Fischer et al., 1997) cloned into the pAcGP67-A vector (BD Biosciences) in frame with an N-terminal gp67 signal sequence and a C-terminal hexahistine tag was produced using the baculoviral expression system ...
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bioRxiv - Developmental Biology 2020Quote: Nucleated bone marrow cells were plated into tissue culture 6 well plates (BD Falcon) at a density of <105 cells/cm2 ...