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Citations for Becton, Dickinson and Company :
1351 - 1400 of 7150 citations for 6 Bromo 1 2 cyanoethyl 2 4 diethylamino phenyl benz cd indolium chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... digested cells were resuspended in FACS buffer (2% FBS–PBS) and analyzed by a FACS Calibur flow cytometer (Becton, Dickinson and Company, USA). Compensation and analysis of results were performed using Flow Jo software.
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed for 10 min in 2% paraformaldehyde and were analyzed using a FACS CANTO II (BD Biosciences, Franklin Lakes, NJ, USA). For lentiviral transduction efficiency assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... of both preparations was determined by titration onto target cell lines and quantification of the percentage of BFP+ cells 2–3 days post-transduction by flow cytometry (BD Biosciences LSR II).
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bioRxiv - Immunology 2023Quote: ... Naïve T cells were resuspended in complete medium (KOHJIN BIO DMEM-H (16003016) + 10% FBS) and activated in the presence of 2 μg/mL of anti-CD3 (BD Pharmingen, REF: 553058) and 2 μg/mL of anti-CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 2x in FACS buffer (5% FBS, 2 mM EDTA in PBS) and resuspended with anti-CD16/CD32 Mouse BD FC Block (BD Biosciences, Cat. 553142) in FACS buffer at a concentration of 10 ug/ml for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... the internal space of the chambers in which biofilms were incubated measured 5,000 μm × 500 μm × 70 μm (LxWxH). Segments of inlet tubing (Cole Parmer PTFE #30, cat. # 06417–11) attached to 27Gx1/2 needles (BD Precision, cat. # 305109) on 1 milliliter syringes (Brandzig ...
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bioRxiv - Microbiology 2023Quote: ... We then washed the cells and stained with FACS antibodies (anti-IL-2 FITC [BD Biosciences, 562040], anti-TNF PE [BD Biosciences, 554419], anti-IFN-γ [BD Biosciences, 557735], and anti-CD107a [BD Biosciences, 560647]) by 20 minutes of incubation at room temperature ...
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bioRxiv - Immunology 2022Quote: ... CHO cells expressing CTLA-4 WT or CTLA-4 Del36 were surface CTLA-4 stained on ice with anti-CTLA4-PE (BD Biosciences, clone BNI3) for 60 min ...
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bioRxiv - Immunology 2021Quote: ... or for 4 h with 20 ng/ml recombinant mouse IL-4 (BD Pharmigen). Cells were then washed twice with isotonic buffer composed of 147 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Potential suppressive T cell populations were obtained from spleens of infected C57BL/6 mice at day 6 post-infection and sorted using a FACS Aria II (BD Bioscience, Franklin Lakes, New Jersey). 5×104 naïve target CD4+ or CD8+ T cells were seeded in complete RPMI 1640 (RPMI 1640 + 5 % FCS + 2 mM L-glutamine + 10 mM HEPES + 50 μg/mL Gentamicin ...
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bioRxiv - Cancer Biology 2021Quote: Activated OTI T-cells were combined with MC38-OVA tumor cells at a 1:4 effector:target ratio and cultured together with 0.6μL/mL GolgiStop (BD Biosciences) for 5 hours ...
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bioRxiv - Microbiology 2022Quote: ... Strains were grown and maintained at 32 °C on Casitone-yeast extract (CYE) (1% casitone, 0.5% yeast extract, 10 mM MOPS [pH 7.5], 4 mM MgSO4) 1.5% agar (BD Difco) plates or in CYE liquid medium at 32 °C on a rotary shaker at 220 rpm.
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bioRxiv - Biochemistry 2022Quote: ... The retinas were then incubated overnight at 4℃ with a monoclonal VE-cadherin antibody (1:100, BD Biosciences) and probed for 2 h with an FITC-conjugated goat anti-rat antibody (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for immunoblot were as follows: AURKA (1:1000; mouse mAb clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C [1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Staining was performed overnight at 4°C with anti-Pax6-A488 (1:200, BD Biosciences, Cat. N: 561664), anti-Eomes-PE (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies for immunoblot were as follows: AURKA mouse mAb (1:1000; Clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies for immunoblot were as follows: AURKA mouse mAb (1:1000; Clone 4/IAK1, BD Transduction Laboratories), phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mice were intraperitoneally injected with 1 ml of 4% Brewer’s modified thioglycolate medium Brewer Modified (BD company, USA). Four days later ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was incubated overnight at 4°C with mouse anti-LR11 (1:500, #612633, BD Transduction Laboratories) for SORLA detection ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4°C with primary antibodies: rat anti-CD11b (1/100, 550282, BD-Biosciences), rabbit anti-SARS-CoV nucleoprotein (1/500 ...
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bioRxiv - Immunology 2019Quote: ... Cells were stained with fluorescently labeled antibodies (1:200) (Table 4) and sorted on FACSAria Ilu (BD Biosciences), FACSAria Fusion (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then diluted 1:4 in annexin binding buffer and analyzed in a flow cytometer (BD-LSRII). Cell populations were gated using forward scatter/side scatter ...
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bioRxiv - Pathology 2019Quote: ... the heart sections were incubated overnight at 4°C with anti-CD41 antibody (1:100, 553897; BD Pharmingen), anti-alpha smooth muscle actin antibody (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... cecal slurry was saved at a 4:1 ratio in Luria Bertani (LB) broth (BD Difco, MD, USA) containing 30% glycerol at –80°C ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCS were stained with PerCP-Cy5.5 mouse anti-Oct3/4 (1:20, #51-9006267, BD Pharmingen, USA), PE mouse anti-human Pax-6 (1:20 ...
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bioRxiv - Physiology 2022Quote: ... sections were fixed in 4% paraformaldehyde for 10 min and then stained with CD31 (1:500, BD Pharmingen), myosin heavy chain [1:5 ...
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bioRxiv - Microbiology 2023Quote: ... The animals (12-14 weeks post-engraftment) were treated with 1 ml of 4% thioglycolate (Brewer’s medium; BD) via intraperitoneal (IP ...
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bioRxiv - Immunology 2023Quote: ... see Supplementary Table 1) for 30 min at 4°C and washed twice in TF washing buffer (BD) and once in SB.
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated overnight at 4°C with anti-CD9 (1:1000, BD Pharmingen, San Jose, CA, USA), anti-CD63 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... All samples were incubated at 4°C with rat anti-mouse CD326 (EpCAM, BD Pharmingen, 552370, 1:1,000) and 10 μg/ml Hoechst 33342 (Molecular Probes/Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Cells or purified ApoBDs were lysed at 4°C using ice-cold 1× cell lysis buffer (BD Biosciences). Protein concentration of the ApoBD lysates was determined using Pierce™ bicinchoninic acid assay (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... They were incubated overnight at 4 °C with mouse anti-BrdU antibody (1:500 dilution, BD Biosciences, 347580) under non-denaturing conditions followed by anti-mouse secondary antibody (1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... CTLA-4 PECy5 (BNI3, BD), and LAG-3 PE (polyclonal ...
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bioRxiv - Immunology 2020Quote: ... brewer modified (4%; BD Biosciences), and recovered 4 d later by peritoneal lavage with 5 ml phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... and OCT-4 (BD Biosciences) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (11B11; BD Biosciences), IFNγ (XMG1.2 ...
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bioRxiv - Neuroscience 2021Quote: ... Stimulated cells were stained with fluorochrome-conjugated anti-MCP-1 and anti-IL-6 Abs using the Cytofix/Cytoperm kit (BD Biosciences, San Jose, CA) to determine MCP-1 and IL-6 production ...
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bioRxiv - Developmental Biology 2021Quote: Mouse ESCs were pre-differentiated to an epiblast-like state as previously described [2] by seeding fibronectin-coated dishes (BD Biosciences cat. no. 356008) at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat ...
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bioRxiv - Plant Biology 2019Quote: ... Chromosomal DNA was then stained by 4’,6-diamidine-2’-phenylindole (DAPI) at final concentration 2 μg/ml and the chromosome suspensions were analysed by FACSAria SORP II flow sorter (BD Biosciences, San Jose, USA) at rates of 1000-2000 particles/sec ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS 2% FBS and analyzed by flow cytometry using a FACSCanto and Novocyte flow cytometer (BD Biosciences and Acea Biosciences). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Developmental Biology 2020Quote: Cryopreserved cells were thawed at room temperature and washed twice with 10 ml of FACS buffer (PBS and 2% FBS) and filtered through a 100 µM filter (BD BioSciences, cat no: 352360). Cell concentration was determined by hemocytometer ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 2% formaldehyde solution (Tousimis, Rockville MD) and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience, San Jose, CA). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
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bioRxiv - Biochemistry 2022Quote: ... either single GFP+ cells (to obtain clones 1 and 2) or 250 GFP+ cells (to obtain the pool) were sorted per well into a 96 well plate (BD FACSAria Fusion Cell Sorter). The two clones and the pool were expanded and the decreased ArgBP2 expression was confirmed by immunoblotting.
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bioRxiv - Immunology 2019Quote: ... The cells were then washed twice in media and resuspended in 2% paraformaldehyde until they were run on a BD LSRII Flow Cytometer (BD BioSciences, San Jose, CA). Flow data were analyzed using Flowjo version 10.5.3.
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bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed using 2% paraformaldehyde (PFA) solution for 30 minutes and permeabilization was performed using a 1X BD perm/wash solution (BD Biosciences, USA; cat#554723) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were co-cultured in R10 medium alone or in presence of K562 cells for 2 hours in the presence of anti-human CD107a (FITC or Brilliant Violet 421, H4A3, BD Biosciences, San Jose, Calif.).
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the cocktail for surface staining: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2, Cat#557757, BD Pharmingen, San Jose, CA), CD4-PE-CF594 (clone L200 ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs were assessed to determine the frequency of circulating T follicular helper (Tfh) using a panel which included the following antibodies: CD3 (BD Biosciences; clone SP34-2), CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: All mycobacterial strains (Supplementary File 2 – Key Reagents) were grown in liquid culture containing Difco™ Middlebrook 7H9 Broth (BD Biosciences, San Jose, CA) and supplemented with 0.2 % (v/v ...