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Citations for Becton, Dickinson and Company :
1351 - 1400 of 1658 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... for 16 hours followed by stimulation with a peptide pool of a broad range of HLA-subtypes and infectious agents (CEFX Ultra SuperStim Pool; JPT) (1 µg/ml) and 1 μg/ml mouse-anti-human CD28 antibody (#556620; BD Biosciences), or IL-2 (20 IU/ml ...
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bioRxiv - Genomics 2024Quote: ... day 7 cells were transferred to fresh culture medium with IL-2 and stimulated again with peptides and 1ug/mL of anti-human CD28 antibody (BD Biosciences) for another 7 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then spun down and resuspended in 100LμL of buffer containing fluorescently conjugated antibodies for one hour (5LμL of each antibody per sample): APC-Cy7 Mouse Anti- Human CD4 (1:20 dilution; BD Biosciences, #557871), APC-Cy7 Mouse IgG1 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed 2x with 1% FBS in PBS and incubated in 1% FBS in PBS with APC-conjugated mouse anti-human CD68 (BD Biosciences) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... bone marrow aspirates were performed every 6 weeks post-transplant and human chimerism was tracked by flow cytometry analysis (using a BD LSR Fortessa analyzer or BD FACSymphony) and analyzed with the following ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: mouse anti-human CD3 conjugated to BUV737 (clone UCHT1, BD, 612750), mouse anti-human CD45 conjugated to FITC (clone HI30 ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL, BD Biosciences, Franklin Lakes, NJ, USA). A 1:1,000 dilution of anti-mouse IgG Alexa-647 (stock 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: Specificity of neoantigen-reactive CD8+ T-cells and TCR-transduced T-cells was assessed by human IFNγ (BD Biosciences Cat. #555142), IL-2 (BD Biosciences Cat ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor (TNF) in cell supernatants were measured using the Human Inflammatory Cytokine Cytometric Bead Array Kit (BD Biosciences). Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... Mouse and rat anti-human monoclonal antibodies (mAbs) used in this study included: IFNγ FITC (clone 25723.11, BD Biosciences, cat# 340449), TCRαβ Percp 710 (clone IP26 ...
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bioRxiv - Cancer Biology 2022Quote: The fraction of CD44+/CD24− cells was determined by trypsinizing and resuspending cells in FACS buffer at 10×106 cells/mL followed by incubation with antibodies against human CD44 (APC-conjugated, Clone G44-26, 1:5, BD Biosciences) and CD24 (PE-Cy7-conjugated ...
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bioRxiv - Cancer Biology 2022Quote: ... and engraftment of human cells in the bone marrow and spleen was measured with flow cytometry (CD45-PE-Cy7 antibody, BD Biosciences). Single-cell suspensions were prepared from the bone marrow ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of CD4+ T cells was carried out by negative selection using the Human CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences). All patient samples were obtained with informed consent for research use ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary AML cells were harvested and stained with human CD45 and cells were analyzed by a high-throughput automated plate reader (BD LSRFortessa). Dead cells were excluded using the Near-IR Live/Dead marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Human and Non-human primate samples were processed similarly: Blood was collected in 8 mL sodium citrate-containing CPT tubes (BD Biosciences) and separated into plasma and peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2023Quote: ... BM aspirate samples from heathy donors using a Ficoll density gradient and stained with the following anti-human antibodies: IgD-Brilliant Violet 480 (Cat. No. 566138; BD Biosciences), CD3-BUV737 (Cat ...
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bioRxiv - Immunology 2023Quote: ... Cytokine concentrations in the supernatants of DC cultures were measured using a BD human inflammatory cytometric bead array kit (BD #551811) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were then harvested and incubated in a blocking buffer containing 10%FBS and 1μg/mL Human Fc block (BD Biosciences, USA) for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were activated for 3 days with 1 μg/mL/million cells of purified NA/LE mouse anti-human CD3 and CD28 (BD Biosciences) and infected with HIVBaL at 0.01 ng/106 cells ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... and stained for 30 min at 4 °C with the following antibody mixture: PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Fixed cells were stained for 30 min at 4° C with PerCP-Cy5.5 mouse anti-human CD195 (CCR5, BD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... IL-2 levels in the co-culture supernatant were quantified using ELISA (BD OptiEIA Human IL-2 ELISA kit, BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Cells were labelled with unique sample identifier tags (Sample Tag 1-12) using the BD Human Single-Cell Sample Multiplexing Kit (633781/BD Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... ∼1.0 × 106 cells treated with or without 200 ng/ml dox were stained with APC Mouse Anti-Human IgM (BD 551062) diluted 1:100 in FACS Buffer (1× PBS with 2% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... was determined using flow cytometry after incubation with a PE-conjugated anti-human CD25 antibody (cat. 555432, BD Biosciences, BD PharMingen).
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bioRxiv - Bioengineering 2024Quote: ... was determined using flow cytometry after incubation with a PE-conjugated anti-human CD25 antibody (cat. 555432, BD Biosciences, BD PharMingen).
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with PBS/1% BSA for 30 min at 4 °C for blocking unspecific binding and washed with PBS prior to the incubation with PE-conjugated anti-human CD69 antibody (cat. 555531, BD Biosciences, BD PharMingen) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed with the kit washing buffer and stained with the intracellular mix containing the following anti-human antibodies: Ki67 (BUV395, 1/160, BD Biosciences) and Foxp3 (AF488 ...
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bioRxiv - Immunology 2024Quote: ... with regular verification of the depletion step by staining the incubated cells with an anti-human IgG fluorescent antibody and running these cells on the LSRII flow cytometer (BD Biosciences) (Figure 1A) ...
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bioRxiv - Immunology 2024Quote: ... Cells were further stained intracellularly with antibodies against anti-human CD3 (APC-Cy7, clone: SP34-2, BD, cat# 557757, 1:200), anti-human IFNγ (AF700 ...
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bioRxiv - Immunology 2024Quote: ... was used to isolate the neutrophil population (verified by flow cytometry using an APC-cy7 antibody specific for human CD16 (clone 3G8, BD Biosciences) and a PE antibody for human CD66b (clone C10F5 ...
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bioRxiv - Immunology 2024Quote: ... and each tube was stained with 5 μL of APC-Cy7 Mouse Anti-Human CD31 (PECAM-1) antibody (clone WM59, BD Biosciences) and 5 μL of APC anti-human CD144 (VE-Cadherin ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue and cell slides were then blocked with 1% BSA and 10% normal goat serum in PBS at room temperature for 1 hour followed by overnight incubation at 4°C with primary antibodies (1:50 dilution) against human HIF1α (cat # 610958, BD Biosciences), rat HIF1α (cat # NB100-105 ...
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bioRxiv - Microbiology 2024Quote: ... the indicated cytokine cytometric beads and PE-conjugated detection antibodies from the BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit (BD Bioscience) were mixed with LPS treated THP-1 supernatants according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... CD81/CD274 expression profiles were measured 5 days post-infection of dual MS2/PP7 sgRNAs following staining with antibodies against both targets (APC Mouse Anti-Human CD81 antibody (Cat# 561958, BD Bioscience) and Alexa Fluor 488 Mouse Anti-Human CD274 antibody (Cat# 53-5983-42 ...
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bioRxiv - Cancer Biology 2024Quote: ... detecting the ratio of human CD45 (labelled with PE) to mouse CD45 (labelled with APC-Cy7) on a LSRFortessa instrument (BD Biosciences). Upon detection of engraftment oral administration of 0.033% or 0.1% VTP50469 (supplemented rodent special diet ...
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bioRxiv - Cell Biology 2024Quote: Human samples were incubated in the dark at room temperature with 1.2 µL of an equal mix of BD Pharmingen™ PE Mouse Anti-Human CD62P (BD Biosciences) and BD™ FITC Mouse Anti-Human PAC-1 (#340507 ...
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bioRxiv - Immunology 2020Quote: ... and proteins surface expression assessed by Accuri C6 Plus flow cytometry (BD Bioscience, Oxford, UK). Cytokines and chemokines were measured in the cell media supernatant by ELISA (Peprotech) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells expressing fluorescence proteins were collected uisng a FACS Aria III flow cytometer (BD Biosciences), and used for downstream experiments.
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bioRxiv - Neuroscience 2021Quote: ... C-terminal-binding protein 2 / RIBEYE ribbon synapse maker (Schmitz et al., 2000) (CtBP2, BD Bioscience ...
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bioRxiv - Immunology 2020Quote: Surface marker proteins and intracellular cytokine responses were quantified via flow cytometry (LSRII, BD Biosciences) and analyzed using FlowJo software version 10.5.3 (TreeStar Inc. ...
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bioRxiv - Immunology 2021Quote: ... Cell surface expression of adhesion molecule protein was measured by multiparameter flow cytometry (BD Fortessa). Supernatants were retained and secreted chemokines were measured by ELISA (IP-10 ...
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bioRxiv - Immunology 2020Quote: ... Expression of proteins was measured using FACS Canto II HTS (BD Biosciences, Bedford, MA, USA) and analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2020Quote: ... HA proteins were tetramerized through sequential addition of streptavidin-BV650 or streptavidin-BV785 (BD biosciences), with HA in excess to streptavidin ...
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bioRxiv - Microbiology 2021Quote: ... Expression levels of surface S proteins were analyzed using a FACS Canto II (BD Biosciences). RL activity was normalized to the mean fluorescence intensity (MFI ...
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bioRxiv - Plant Biology 2021Quote: WRKY proteins used for this experiment were fused into pGBT9 vector (containing GAL4 DNA-BD) and transformed into yeast strain Y190 ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...
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bioRxiv - Immunology 2021Quote: ... protein transport inhibitor (BD Golgi Plug™ containing Brefeldin A, 1 µg/mL and BD Golgi Stop™ containing monensin ...