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Citations for Becton, Dickinson and Company :
1301 - 1350 of 6939 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were stained for surface markers (when required) then fixed/permeabilized by using PE Active Caspase-3 Apoptosis Kit (BD Bioscience, 550914) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Mammary fat pad injections were performed in the 4th pre-cleared mammary fat pad of 3 weeks old female mice in 50% matrigel (BD Biosciences, Germany). Breast or prostate cancer-origin of xenografts were verified by a pathologist.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2022Quote: ... PBMC from the 3-time point from each patient were labelled using Single Cell Labelling with the BD Single-Cell Multiplexing Kit (BD Biosciences, #633781) and 52 different BD AbSeq Ab-Oligos reagents following the manufacturer’s protocol (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in 500 μl PBS with 3 % FBS and then detected by flow cytometry (BD Biosciences, San Diego, CA, USA).
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Immunology 2020Quote: ... and 3-Iodo-L-tyrosine-treated B cells were labeled by using the Annexin V Fitc Apoptosis detection kit (BD, Heidelberg, Germany) according to manufacturer`s instructions ...
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bioRxiv - Immunology 2022Quote: T cells were isolated as described above from the intestine of 3 individual samples and PLZF+ CD4+ TCR-β+ T cells were sorted on proxy surface markers using FACSAria2 SORP (BD Biosciences) as previously described (14) ...
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bioRxiv - Molecular Biology 2022Quote: ... The dermis was incubated in the enzyme mix from the whole skin dissociation kit (Milteney Biotec) for 3 hours according to the manufacturer’s instructions and further processed by Medicon tissue disruptor (BD Biosciences, Stockholm, Sweden). The dermal cell suspension was incubated with CD90 Microbeads ...
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bioRxiv - Cell Biology 2022Quote: ... maintained for 20 min on ice and then homogenized with syringe every 5 min (BD Microlance™ 3 Needle 0,5 × 25 mm). Nuclear lysate supernatants were collected at 1000 rpm for 10 min at 4°C 43.
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was carried out with the following antibodies diluted in BD Perm/Wash buffer: Active-caspase-3-GranzymeB-PE (C92-605, BD, cat #561011), Active-caspase-3-BV650 (C92-605 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were stained for 30 min on ice with 5 μl of biotin-labeled anti–cleaved caspase-3 monoclonal antibody (BD Biosciences, #550821). The cells were washed in Perm/Wash buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... N2a-ssGT cells were imaged for GFP fluorescence using CytationTM 3 Cell Imaging Multi-Mode Reader (BioTekTM) and flow cytometry analysis by Accuri® C6 flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2024Quote: ... and HIV-1 NL4-3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Microbiology 2024Quote: ... as well as HIV-1 NL4.3-GFP-I virus by plating 1.5 × 106 cells per well of a 6-well flat-bottom plate (BD Bioscience, X, Y) and adding 250 mU RT (equivalent to 45 ng p24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on a 3 laser Cytek Aurora Cytometer (Cytek Biosciences, Fremont, CA) and the data was analyzed using FlowJo software (BD Biosciences, RRID:SCR_008520). All listed antibodies for flow cytometry are from Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was weighed then homogenized for 3 minutes in a bullet blender and serum was added to a serum separator tube (BD Microtainer: 365967) before being spun down and diluted 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Unspecific binding of antibodies was blocked by incubating the samples with 3% rat serum in BD staining buffer (BD Biosciences, Heidelberg, Germany) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and VC3ai cleavage by caspase-3/7 was assessed based on fluorescence emission at 530 nm using a BD LSR Fortessa™ Cell Analyzer (BD Biosciences).
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bioRxiv - Immunology 2024Quote: ... cells were suspended in 0.5 mL PBS+3% FBS and stained for flow cytometry analysis by adding anti-CD16/CD32 antibody (BD Pharmingen; clone 2.4G2) ,anti- CD117 (Biolegend clone 2B8) ...
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bioRxiv - Neuroscience 2024Quote: ... Tracheal intubation was performed after sevoflurane exposure (5%, 3 minutes) using a 20-gauge intravenous catheter (REF 382434; BD Angiocath Plus, Singapore). To ensure proper positioning of the catheter so as to avoid one- lung ventilation and gas leakage during ventilation ...
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bioRxiv - Cancer Biology 2024Quote: ... and CAPAN-1 cells diluted in 200 μl of a 1:1 mixture of Matrigel (BD Biosciences) and PBS (Corning) ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained at 4°C for 15 minutes with an APC-anti-Human-CD62L (clone DREG-56, BD Pharmingen), a BV650-anti-Human-CD11b (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... single cell suspensions were stimulated with OVA peptide (SIINFEKL) 2µM 37°C for 4 hrs in the presence of Golgi Stop (monensin, BD Biosciences). Upon extracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... then cells were collected and 60 μg of sonicated chromatin was incubated overnight at 4°C with 20 μl of the anti-BrdU antibody (347580 Pure BD). Then ...
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bioRxiv - Neuroscience 2022Quote: ... and the following antibody mixture in PBS at 4°C for 30 minutes: BV421 Rat Anti-CD11b (BD Biosciences 562605), BV510 Hamster Anti-Mouse TCR β Chain (BD Biosciences 563221) ...
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bioRxiv - Immunology 2021Quote: ... for 4 h at 37°C in the presence of both Brefeldin A (GolgiPlug) and Monensin (GolgiStop, both BD Biosciences). As GolgiPlug and GolgiStop were used together ...
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bioRxiv - Immunology 2020Quote: ... In experiments testing double recombinants SARS-CoV2 vectors IL-2 antibody was not included and PE-CF594 anti-mouse IL-4 (clone 11B11, 562450, BD) and BV421 rat anti mouse IL-10 (clone JES5-16E3 ...
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bioRxiv - Immunology 2021Quote: ... followed by 200 μL FACS buffer before resuspending the cells in FACS buffer for acquisition in a high-throughput sampler on a 4-laser LSR Fortessa (BD), at a flow rate of 1 μL/s.
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bioRxiv - Immunology 2021Quote: ... cells from tumors were cultured ex-vivo from tumors for 4 hours in the presence of Golgi Plug (BD Biosciences) prior to cell surface and intracellular staining.
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bioRxiv - Immunology 2020Quote: ... for 2 h and 4 h respectively at 37°C in 5% CO2 and exposed to brefeldin A (BD Biosciences) as described in previous studies ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cells were washed again and re-suspended in permeabilization buffer with the following fluorescently-tagged antibodies targeting intracellular T-lymphocyte proteins for 30 min: IL-4 BV 421 (BD Biosciences clone 11B11 ...
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bioRxiv - Genomics 2021Quote: ... stimulated cells were stored at 4°C until staining with combinations of fluorochrome-conjugated monoclonal antibodies including: anti-CD3 (SP34-2: Alexa700; BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed twice (PBS) and fixed with 500 μl of 4% PFA and analysed by flow cytometry (BD Bioscience). A total of 10,000 events were acquired and data were analysed using FlowJo software version 10.6.2.
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with the following antibodies for 30 min at 4°C: CD38 (Cat#342371; RRID: AB_400453, BD Biosciences), lineage cocktail (CD3/14/16/19/20/56 ...
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bioRxiv - Cancer Biology 2022Quote: Wells with the highest proportion of target barcodes were revived into a 6 well plate and grown for 4 days before being single-cell sorted by BD FACSAria II into 96 well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary or conjugated antibodies were incubated with the cells overnight at 4°C in Perm/Wash buffer (554714, BD Biosciences) containing 4% FBS and secondary antibodies for 45 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation for 25 min at 4° C with the following mouse specific antibodies to delineate subpopulations: CD24-PE (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... The cells (2-4 x 105 cells per well of a 96-well plate) were added to the ELISpot plate containing an immobilized primary antibody to either IFN-g or IL-4 (BD Cat# 551881 and BD Cat# 551878 ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... and stained with the following cocktail for 30 minutes at 4°C in the dark to enable delineation of mature mononuclear phagocytes: Anti-CD45 PE-Cy7 (BD), anti-HLA-DR eFluor 450 (eBioscience) ...
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bioRxiv - Immunology 2022Quote: ... Antibodies to intracellular proteins were added to samples for 20 minutes at 4°C in the dark and then washed with PermWash (BD). Flow cytometry analysis was performed using a LSRFortessa flow cytometer (BD ...
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bioRxiv - Biophysics 2020Quote: ... The samples were kept in the dark at 4°C until analyzed using a LSR II flow cytometer (BD Biosciences). Hoechst 33342 and Pyronin Y staining were measured with a UV (350 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... generated by hanging drops culturing of ESCs (1,000 cells per drop) were collected after 4 days and embedded in growth-factor-restricted Matrigel (MTG; BD Biosciences); 50µl MTG drops (containing around 20 EBs ...
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bioRxiv - Cell Biology 2020Quote: ... intranuclear staining was performed at 4 °C overnight in the dark using the FITC anti–human Ki-67 antibody (BD) diluted in Perm/Wash ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... and labeling at 4°C for 15 minutes with the following specific antibodies: rat APC.Cy7 anti-mouse CD45 (BD Pharmingen) 1:100 ...
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bioRxiv - Immunology 2022Quote: ... then fixed in 2% paraformaldehyde and stored at 4°C until acquisition on a FACSAria Fusion III flow cytometer (BD). Flow cytometry data was analysed with FlowJo version 9.9.6 (Tree Star).
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bioRxiv - Immunology 2022Quote: ... Fluorescent bead uptake was measured in 4% paraformaldehyde (PFA) fixed cells by flow cytometry on a BD LSRII (BD Biosciences) and analyzed by FlowJo 10.3 ...
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bioRxiv - Immunology 2022Quote: ... were used to stain the cells in flow cytometry staining (FACS) buffer for 30 min at 4 °C before being washed and analyzed by BD FACS Array software™ on a BD FACS Array flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with antibodies against surface antigens for 20 minutes at 4°C and fixed with Stabilising Fixative (BD) prior to acquisition (52) ...
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bioRxiv - Immunology 2020Quote: ... BV421 conjugated streptavidin was added for 20 minutes at 4 °C prior to fixation and permeabilisation (BD Fix perm kit). Anti IFN-γ-FITC (clone CC302 ...